Optimization of Fermentation Medium and Culture Conditions of Hydrocarbon-Degrading Bacterium Brevibacillus panacihumi W25

2014 ◽  
Vol 1010-1012 ◽  
pp. 69-72
Author(s):  
Xin Xin Wang ◽  
Chen Li ◽  
Li Bin Zhao ◽  
Liang Wu ◽  
Ping Zhou ◽  
...  
Keyword(s):  
Contaminated Soils ◽  
Polynomial Regression ◽  
Ph Value ◽  
Fermentation Medium ◽  
Culture Conditions ◽  
Culture Temperature ◽  
Degrading Bacterium ◽  
Initial Ph ◽  
Optimization Of Fermentation ◽  
First Time

Members belonging to the genus Brevibacillus had potentials in the bioremediation of hydrocarbon-contaminated soils. However, little is known about the fermentation medium and culture conditions of Brevibacillus panacihumi. The effect of concentrations of glucose and (NH4)2SO4, initial pH value and culture temperature on the biomass of B. panacihumi W25 was measured with single factor experiments, which was a hydrocarbon-degrading bacterium. Polynomial regression analysis showed optimal concentration of glucose and (NH4)2SO4, initial pH value and culture temperature were 45.2 g/L, 0.6 g/L, pH 7.3 and 30.3°C, respectively. The optimization of fermentation of B. panacihumi was reported for the first time.

Study on Optimization of Fermentation Condition for Nitrilase-Producer Escherichia Coli BL21 (DE3)/pET-Nit

2011 ◽  
Vol 175-176 ◽  
pp. 192-196 ◽  
Author(s):  
Li Li Feng ◽  
Jian Fei Zhang ◽  
Hui Luo ◽  
Zheng Li ◽  
Hong Jie Zhang
Keyword(s):  
Escherichia Coli ◽  
Recombinant Strain ◽  
Culture Medium ◽  
Culture Conditions ◽  
Fermentation Condition ◽  
Hydrogen Phosphate ◽  
Strain Bl21 ◽  
Initial Ph ◽  
Potassium Hydrogen ◽  
Optimization Of Fermentation

The paper concentrated on the optimization of the recombinant strain BL21 (DE3)-PE7-Nit. The component of culture medium and the culture conditions were optimized. The optimized medium was: yeast extract 10 g/l, L-glutamate sodium 8 g/l, MgSO4.7H2O 0.7 g/l, Isopropyl-β-D-thiogalactopyranoside 0.3 mmol/L, potassium hydrogen phosphate 0.5 g / L, phosphate Potassium 0.5 g / L and the culture condition was: initial pH 7.0, inoculum 2%. The result showed that the activity of nitrilase prepared with these conditions increased by 130.37 % through optimization.

Research on the Characteristics and Culture Conditions of Saccharomyces boulardii

2011 ◽  
Vol 343-344 ◽  
pp. 594-598
Author(s):  
Li Ping Du ◽  
Rui Xue Hao ◽  
Dong Guang Xiao ◽  
Li Li Guo ◽  
Wei Dong Gai
Keyword(s):  
Ph Value ◽  
Acid Tolerance ◽  
Saccharomyces Boulardii ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Flask Culture ◽  
Culture Temperature ◽  
Highly Active ◽  
Physiological And Biochemical Characteristics ◽  
Thermal Death

The morphological, physiological and biochemical characteristics of Saccharomyces boulardii were studied for the preparation of highly active yeast. The optimal condition of the flask culture including inoculum size, culture temperature, pH value, were investigated separately. The results showed that S.boulardii was asporogenous and had better heat tolerance and acid tolerance growing well at 37°C and pH 2.0. We also found that thermal death temperature was 55~56°C and S.boulardii is well ethanol-tolerant, for the highest concentration of ethanol that S.boulardii can tolerate was 20%. The optimal culture conditions in a flask culture was YEPD medium with 50ml/250ml medium volume, natural pH, 5% inoculum size, 32°C culture temperature, 160 r/min shaker speed, and 24 hours cultivation period. Under these conditions, suspension was diluted 10 times and the optical density at 600 nm was up to 0.85.

scholarly journals Isolation of Xylanase Producing Strains, Optimization of Fermentation Conditions and Research on Enzymatic Properties

2021 ◽  
Vol 12 (2) ◽  
pp. 1
Author(s):  
Ji Huilong ◽  
Gao Xin ◽  
WU Wenxuan ◽  
Ma Zhuang ◽  
Qing Qing
Keyword(s):  
Enzyme Activity ◽  
Specific Activity ◽  
Ph Value ◽  
Fermentation Broth ◽  
Sodium Dodecyl ◽  
Alkali Resistance ◽  
Initial Ph ◽  
Acid And Alkali Resistance ◽  
Optimal Reaction Temperature ◽  
Optimization Of Fermentation

In this study, we successfully isolated a strain of Aspergillus oryzae TR08, which produced xylanase secreted to the outside of the cell productively. The enzyme activity and specific activity in the fermentation broth of this strain reached peak values of 451 IU/mL and 1963 IU/mg after 156 h of fermentation. A single factor experiment was designed, and it was found that the strain was adjusted to the initial pH of the fermentation broth to 7.5 in a shaker at 180 rpm and 32 °C. After 156 h of fermentation, the enzyme activity reached a maximum of 1264 IU/mL. The optimal reaction temperature and pH value of the xylanase were 55 °C and 7.5, respectively, and it had excellent acid and alkali resistance and a wide pH activity range. The xylanase was increased the catalytic activity by 15% in 0.25 mM Fe3+, and the biological activity of the enzyme was not affected in the sodium dodecyl sulfate environment.

scholarly journals Optimization of Culture Conditions for Amoxicillin Degrading Bacteria Screened from Pig Manure

2020 ◽  
Vol 17 (6) ◽  
pp. 1973 ◽  
Author(s):  
Xuanjiang Yang ◽  
Panpan Guo ◽  
Miao Li ◽  
Hualong Li ◽  
Zelin Hu ◽  
...  
Keyword(s):  
Response Surface ◽  
Ph Value ◽  
Culture Conditions ◽  
Pig Manure ◽  
Bacterial Strains ◽  
Inoculum Level ◽  
Fermentation Conditions ◽  
Culture Time ◽  
Degrading Bacteria ◽  
Initial Ph

(1) Objective: The objective of this study was to screen amoxicillin (AMX)-degrading bacterial strains in pig manure and optimize the fermentation conditions for these strains to achieve high fermentation rate, which can provide an effective way for the practical application of bacterial strains as antibiotic-degrading bacterial in treating livestock waste for antibiotic residues. (2) Methods: Antibiotic susceptibility tests and high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) were employed to screen AMX-degrading bacterial strains in pig manure. The culture conditions were optimized for AMX-degrading bacterial strains using Plackeet–Burman design (PBD), the steepest ascent design, and the response surface methods, coupled with the Box–Behnken design (BBD). The effects of culture time, temperature, rotator (mixing) speed, inoculum level, and initial pH value on the growth of AMX-degrading strains were investigated. Experimental data obtained from BBD were utilized to generate a second-order polynomial regression model for evaluating the effects of the tested variables on the optical density at 600 nm (OD600) of culture solutions as the growth indicator for the screened AMX-degrading strains. (3) Results: The initial pH, culture time, and the inoculum level had significant effects on the OD600 value (growth) of the screened AMX-degrading strains. The initial pH value was found to be the most critical factor influencing the growth of bacteria. The optimized culture condition for the bacterial growth determined by the response surface methodology was: the initial pH of 6.9, culture time of 52 h, and inoculum level of 2%. The average OD value of 12 different fermentation conditions in the initial fermentation tests in this study was 1.72 and the optimization resulted in an OD value of 3.00. The verification experiment resulted in an OD value of 2.94, which confirmed the adequacy of the optimization model for the determining the optimal culture condition. (4) Conclusions: The growth of the screened strain of AMX-degrading bacteria could be optimized by changing the fermentation conditions. The optimization could be achieved by using the Box–Behnken response surface method and Plackett–Burman experimental design.

RESEARCH OF MICROBIOLOGICAL SYNTHESIS OF FATS IN BEET MOLASSES SOLUTIONS

2020 ◽  
Author(s):  
С.О. СЕМЕНИХИН ◽  
О.В. ФЕДОСЕЕВА ◽  
М.В. БАБАКИНА ◽  
Н.М. ДАИШЕВА
Keyword(s):  
Ph Value ◽  
Matter Content ◽  
Biologically Active ◽  
Dry Matter Content ◽  
Microbiological Synthesis ◽  
Vital Activity ◽  
Beet Molasses ◽  
Initial Ph ◽  
First Time ◽  
Active Substances

Проведены исследования обработки растворов свекловичной мелассы 4 штаммами микроорганизмов с целью обогащения растворов жирами. Выявлено влияние начального значения рН, а также оптимального содержания сухих веществ в растворах мелассы, обеспечивающие эффективную жизнедеятельность микроорганизмов с синтезом биологически активных веществ. Установлено, что штаммы R. glutinis Y-332, C. curvatus Y-2236 и U. isabellina F-526 в растворах мелассы с начальным значением рН 8,5 синтезировали жиры в количестве 0,015 0,017 и 0,007 г/г сахаров соответственно штамм R. toruloides Y-334 в растворе мелассы с начальным значением рН 7,0 синтезировал жиры в количестве 0,004 г/г сахаров. Впервые получены экспериментальные данные, позволившие оценить эффективность микробиологического синтеза жиров в растворах мелассы без дополнительного внесения биогенных веществ, а также без поддержания оптимальной рН среды 4 видов микроорганизмов. Обосновано, что штаммы R. glutinis Y-332 и C. curvatus Y-2236 являются наиболее перспективными для обогащения растворов свекловичной мелассы жирами. Research aimed at treatment of beet molasses solutions with 4 strains of microorganisms in order to enrich the solutions with fats has beed established. The influence of the initial pH value, as well as the optimal dry matter content in molasses solutions, which ensure the effective vital activity of microorganisms with the synthesis of biologically active substances, is revealed. It was established that the strains of R. glutinis Y-332, C. curvatus Y-2236 and U. isabellina F-526 in molasses solutions with an initial pH of 8,5 synthesize fats in an amount of 0,015 0,017 and 0,007 g/g of sugars, respectively and the strain of R. toruloides Y-334 in molasses solution with an initial pH value of 7,0 synthesizes fats in an amount of 0,004 g/g of sugars. For the first time, it was obtained the experimental data that made possible to evaluate effectiveness of microbiological synthesis of fats in molasses solutions without nutrient addition, as well as without maintaining the optimal pH of the environment for 4 types of microorganisms. It is proved that R. glutinis strain Y-332 and C. curvatus strain Y-2236 are the most promising strains for enriching beet molasses solutions with fats.

Optimization of Culture Conditions for Biosynthesis of Lipopeptide by Bacillus Subtilis with Starch

2013 ◽  
Vol 291-294 ◽  
pp. 225-229 ◽  
Author(s):  
Zong Ming Zheng ◽  
Ti Peng Wang ◽  
Qin Wu
Keyword(s):  
Bacillus Subtilis ◽  
Culture Conditions ◽  
Culture Temperature ◽  
Ph Optimum ◽  
Linear Effect ◽  
Initial Ph ◽  
Culture Cycle ◽  
Optimization Of Culture Conditions ◽  
Fermentation Parameters ◽  
Main Effects

Optimization of culture conditions for lipopeptide production of lipopeptide by Bacillus subtilis NEL-01 was carried out in shaker flask batch fermentations using composite central design of response surface methodology (RSM). A five-level three-factor central composite design was employed to determine the maximum lipopeptide production at optimum levels for culture temperature, initial pH and culture cycle. Culture temperature and culture cycle showed the significant linear main effects, while pH had no significant linear effect. The production was also significantly affected by quadratic effect of culture temperature and initial pH. Optimum fermentation parameters were predicted at temperature, initial pH, and culture cycle of 34.81 °C, 7.33g/l, 49.26 h, respectively. The prediction lipopeptide yield was 1879.56 mg/l. The subsequent experiments confirmed the prediction.

Study on the Optimization of Fermentation Conditions for Actinomycete F10

2011 ◽  
Vol 480-481 ◽  
pp. 148-152
Author(s):  
Lei Yang ◽  
Guo Ying Zhou ◽  
Guang Tao Song
Keyword(s):  
Antimicrobial Activity ◽  
Dry Weight ◽  
Fermentation Medium ◽  
Fermentation Time ◽  
Nutritional Factors ◽  
Fermentation Conditions ◽  
Antimicrobial Substances ◽  
Initial Ph ◽  
Optimization Of Fermentation ◽  
Fermentation Media

In order to improve the yield of antimicrobial substances extracted from actinomycetes F10 strains fermentation, antimicrobial activity and cell dry weight were taken as the main indicators, we studied the effects on F10 strain’s biological activity of the different fermentation media, different carbon, nitrogen and other nutrients factor, and the initial pH and other non-nutritional factors. The results showed that the optimum fermentation medium is: yeast extract 0.5%, glucose 1%, KH2PO4 0.1%, NaCl 0.1%, CaCO30.3%, pH natural. Strain F10 optimal fermentation conditions were: initial pH 7.0, fermentation time 168h.

scholarly journals Bioconversion of Single Cell Protein from Underutilised Seaweed Turbinaria sp. using Natural Palmyrah Toddy Yeast

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
S Shayanthavi ◽  
R Kapilan
Keyword(s):  
Single Cell ◽  
Crude Protein ◽  
Ph Value ◽  
Fermentation Medium ◽  
Single Cell Protein ◽  
Protein Yield ◽  
Lower Amount ◽  
Cell Protein ◽  
Fermentation Time ◽  
Initial Ph

This study was aimed to produce Single Cell Protein (SCP) from underutilied, abundantly found marine seaweed Turbinaria sp. using natural yeast from palmyrah (Borassus flabellifer) toddy. Turbinaria sp. were collected, sun-dried, ground to powder form and Turbinaria solution was made by mixing distilled water and this was used as a growth medium throughout the experiment without adding any supplements. The experiments were performed in a shaking incubator with the fermentation medium of Turbinaria sp. 100 g/L (10%) inoculated with 10 mL natural palmyrah toddy. Fermentation was carried out at 29o C for 72 h and the crude protein content was determined using Kjeldahl apparatus (38.46%). When the growth temperature was optimised at 35o C, crude protein yield obtaine was significantly increased by 1.36 times than the initial non-optimised condition with temperature 27oC. When fermentation time was optimised as 48 h (44.33%), crude protein yield was significantly increased by 1.14 times than the initial fermentation time (24 h-38.55%). When different combinations of Turbinaria sp. media and inoculum ratio (55:05, 50:10, 40:20, 30:30 and 20:40) were used as medium, crude protein yield was significantly increased by 1.44 times (50:10-43.72%) than the initial media inoculum ratio (55:05-30.21%). When initial pH of the media was changed (5.0, 6.0, 7.0, 8.0 and 9.0), significantly higher relative crude protein yield was obtained with the initial pH value 6.0 (Turbinaria medium-90.97%). Amino acid analysis revealed that the SCP produced from the Turbinaria medium had all the essential amino acids with comparatively higher amount of methionine (3.9%) and lower amount of threonine (0.2%). Vitamin B analysis revealed that SCP yield from Turbinaria medium contained thiamin (0.85 mg/100 g) and riboflavin (3.2 mg/100 g). After the optimisation of culture growing conditions and media composition, SCP production from the excessively grown, underutilised seaweed Turbinaria, was significantly increased by 1.13 times (from 38.46 to 43.72%) with palmyrah toddy mix. Key words: crude protein, palmyrah toddy mix, single cell protein (SCP), Turbinaria sp.

scholarly journals The optimization of fermentation conditions for producing cellulase of Bacillus amyloliquefaciens and its application to goose feed

2017 ◽  
Vol 4 (10) ◽  
pp. 171012 ◽  
Author(s):  
Miao Ye ◽  
Linghong Sun ◽  
Ru Yang ◽  
Zaigui Wang ◽  
KeZong Qi
Keyword(s):  
Incubation Time ◽  
Bacillus Amyloliquefaciens ◽  
Egg Production ◽  
Fertilization Rate ◽  
Culture Conditions ◽  
Liquid Volume ◽  
Hatching Rate ◽  
Culture Temperature ◽  
Egg Weight ◽  
Initial Ph

The proper culture conditions for producing cellulase of Bacillus amyloliquefaciens S1, isolated from the cecum of goose was optimized by single-factor experiment combined with orthogonal test. The properties of the cellulase were investigated by DNS method. The appropriate doses of B. amyloliquefaciens S1 were obtained by adding them to goose feed. It indicated that the suitable culture conditions of producing cellulase were the culture temperature of 37°C, the initial pH of 7.0, the incubation time of 72 h and the loaded liquid volume of 75 ml per 250 ml. The effects of each factor on producing cellulase by B. amyloliquefaciens S1 were as follows: initial pH > incubation time = culture temperature > loaded liquid volume. The optimum reaction temperature and pH were 50°C and 7.0, respectively. This enzyme is a kind of neutral cellulase that possesses resistance to heat and acidity. It showed high activity to absorbent cotton, soya bean meal and filter paper. By adding different doses of B. amyloliquefaciens S1 to the goose feed, it was found that the egg production, average egg weight, fertilization rate and the hatching rate were promoted both in experiment 1 (1.5 g kg −1 ) and experiment 2 (3 g kg −1 ). Also the difference of egg production, fertilization rate and hatching rate between experiment 1 and control group was obvious ( p  < 0.05), and the average egg weight was significantly increased in experiment 2 ( p  < 0.05).

Biodegradation of Swainsonine by Stenotrophomonas maltophilia Strain YLZZ-2 and its Isolation and Identification

2010 ◽  
Vol 178 ◽  
pp. 59-64
Author(s):  
Xing Hua Zhao ◽  
Xin He ◽  
Jian Hua Wang
Keyword(s):  
Stenotrophomonas Maltophilia ◽  
Sole Carbon Source ◽  
Degradation Rate ◽  
Ph Value ◽  
Culture Conditions ◽  
Isolation And Identification ◽  
16S Rdna Sequence ◽  
Degrading Bacteria ◽  
Initial Ph ◽  
Optimal Ph

Eight swainsonine (SW)-degrading bacteria were isolated from the soil where locoweed was buried for 6 months and one of the strains (YLZZ-2) was selected for further study. Based on morphology, physiologic tests, 16S rDNA sequence, and phylogenetic characteristics, the strain showed the greatest similarity to members of the order Stenotrophomonap and the closest to members of the Stenotrophomonas maltophilia group. The ability of the strain to degrade SW, as sole carbon source, was investigated under different culture conditions. The preferential temperature and initial pH value for the strain were 25～35 °C and 6.0～9.0, respectively. The optimal temperature for the strain was 30 °C and the optimal pH value was 7.0. There was positive correlation between degradation rate and inoculation amount. The growth of stain YLZZ-2 and degradation rate were fast, and YLZZ-2 could completely degradate 400 mg/L swainsoine within 24 h. There was a linear relationship between the growth of stain YLZZ-2 and degradation of swainsonine. These results highlight the potential of this bacterium to be used in detoxifying of SW in livestock consuming locoweed.

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