Gill ATPase Activities and Microstructures of Juvenile Chinese Mitten Crab, Eriocheir sinensis, Exposed to Ambient Mercury

2011 ◽  
Vol 183-185 ◽  
pp. 1543-1548
Author(s):  
Yan Min Zhao ◽  
Xin Hua Wang ◽  
Yan Wen Qin ◽  
Bing Hui Zheng ◽  
Lei Zhang

Experiments were carried out to investigate the effects of ambient mercury (Hg2+) on gill Na+-K+-ATPase, Ca2+-Mg2+-ATPase activities, and gill microstructure of juvenile Chinese mitten crab, Eriocheir sinensis. Crabs were exposed to ambient 0.00, 0.01, 0.05, 0.10, 0.20 and 0.30 mg/l Hg2+, and sampled on the days of 1, 7, 14 and 30 respectively. The results showed that both ambient Hg2+ concentrations and exposure time had significant effects on both gill Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities of E. sinensis, which showed the tendency to decrease with ambient mercury concentration increasing and exposure time prolonging. While for crabs exposed to 0.01 mg/l Hg2+, no significant difference was observed when compared with the control. Significant interaction between ambient Hg2+ and exposure time on these two ATPases activities was also observed with the lowest values found in crabs exposed to 0.30 mg/l Hg2+ for 30 days. The anterior gill microstructure analysis of E. sinensis exposed to different ambient Hg2+ for 30 days showed that mercury could alter the gill microstructures of E. sinensis. However, there was no significantly structure change appeared in crabs exposed to 0.01 mg/l Hg2+. Combined, these results indicated that though ambient Hg2+ could lead to significant decrease of both gill Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities, and could destroy the gill microstructures, the crab of E. sinensis could acclimate to low Hg2+ contamination less than 0.01mg/l.

Crustaceana ◽  
2021 ◽  
Vol 94 (1) ◽  
pp. 97-114
Author(s):  
Xiaodong Jiang ◽  
Yuhong Yang ◽  
Yongxu Cheng ◽  
Xugan Wu

Abstract A 6-month feeding trial was conducted to evaluate the hepatopancreas colour, proximate composition, antioxidant capacity, pathogen resistance and growth performance of juvenile Chinese mitten crab Eriocheir sinensis H. Milne Edwards, 1853 fed either formulated diets (FD), traditional diets (TD) or a mixture feeding of traditional diets and frozen trash fish (TF). The results showed that: (1) The hepatopancreas redness (a*) in the FD treatment was significantly higher than that in the other two treatments (). (2) The significantly higher activities of alkaline phosphatase (AKP), acid phosphatase (ACP) and superoxide dismutase (SOD) were observed in the hepatopancreas in the FD treatment, while a significantly higher content of malondialdehyde (MDA) was observed in the haemolymph in the TF treatment (). (3) FD and TD treatment, respectively, had the lowest and the highest mortality after challenging with Aeromonas hydrophila. (4) Females in the FD treatment had a significantly shorter intermoult period in the indoor culture experiment (), no significant difference was observed in weight gain rate, specific growth rate, or survival. In conclusion, formulated diets could enhance the crabseed quality of E. sinensis compared to traditional diets or trash fish.


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