Study on Technology of Isolation and Purification of Corn Germ Glutathione with Macroporous Resin

2011 ◽  
Vol 183-185 ◽  
pp. 306-309
Author(s):  
Li Ping Xu ◽  
Xin Wang ◽  
Peng Cheng Yao
Keyword(s):  
Flow Rate ◽  
Recovery Rate ◽  
Volume Ratio ◽  
Corn Germ ◽  
Analysis Time ◽  
Separation And Purification ◽  
Macroporous Resin ◽  
Isolation And Purification ◽  
Adsorption Time

Study on Macroporous resin purified glutathione of corn germ , determine the best conditions for separation and purification as follows: glutathione and resin volume ratio 4:1, flow rate 1 mL / min ,adsorption time 50 min, elution and resin volume ratio of 4:1, analysis time 120 min, recovery rate of glutathione78.95 %.

Dynamic Absorption and Desorption Research of HPD722 Macroporous Resin for Flavonoids in Thlaspi arvense Linn Extract

2012 ◽  
Vol 602-604 ◽  
pp. 648-655
Author(s):  
Li Sheng Wang ◽  
Rong Kai Ma ◽  
Mei Peng ◽  
Xu Liu ◽  
Hua Yang ◽  
...  
Keyword(s):  
Flow Rate ◽  
Mass Concentration ◽  
Ph Value ◽  
Volume Ratio ◽  
Sample Solution ◽  
Dynamic Adsorption ◽  
Macroporous Resin ◽  
Adsorption And Desorption ◽  
Thlaspi Arvense ◽  
The Impact

The impact of HPD722 macroporous resin on the dynamic adsorption and desorption of flavonoids in Thlaspi arvense Linn extract is studied. Obtained optimal conditions：the mass concentration of the sample solution is selected to be 4 mg•mL-1; while the flow rate of sampling is 1.30mL min-1; pH value is 4；50mL ethanol at a volume ratio of 70% is adopted as the desorption agent；the desorption flow rate reaches 3.47 mL•min-1. Under these conditions, the content of flavonoids increases from 5.09% to 21.91% upon purification by HPD722 macroporous resin.

Dynamic Absorption and Desorption Properties of HPD722 Macroporous Resin for Flavonoids in Emilia sonchifolia Extract

2013 ◽  
Vol 634-638 ◽  
pp. 939-949
Author(s):  
Li Sheng Wang ◽  
Wen Jia Liu ◽  
Yan Hua Zhang ◽  
Li Pang ◽  
Xuan Hai Li
Keyword(s):  
Flow Rate ◽  
Mass Concentration ◽  
Absorption Rate ◽  
Volume Ratio ◽  
Optimal Conditions ◽  
Macroporous Resin ◽  
Height Ratio ◽  
Resin Column ◽  
Sample Flow Rate ◽  
The Impact

The impact of HPD722 macroporous resin on the dynamic adsorption and desorption of flavonoids in Emilia Sonchifolia extract is studied. The diameter-height ratio of macroporous resin column is 1:10; the mass concentration of the sample solution is selected to be 50.00 mg•mL-1; while the sample flow rate is 3.00mL min-1; upon dynamic absorption for once, the absorption rate reaches 81.11%; with adoption of 50mL ethanol at a volume ratio of 60% as the desorption agent, the desorption flow rate comes to 3.00mL•min-1; and the desorption rate of flavonoids attains 85.26%. Under optimal conditions, the content of flavonoids increases from 6.05% to 32.65% upon purification by HPD722 macroporous resin.

scholarly journals Isolation and Purification of Fucoxanthin from Brown Seaweed Sargassum horneri Using Open ODS Column Chromatography and Ethanol Precipitation

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3777
Author(s):  
Yuemei Ye ◽  
Jingwen Sun ◽  
Liting Wang ◽  
Junwang Zhu ◽  
Wei Cui ◽  
...  
Keyword(s):  
Column Chromatography ◽  
Recovery Rate ◽  
Biological Activities ◽  
Brown Seaweed ◽  
Sargassum Horneri ◽  
Scale Production ◽  
Water Mixture ◽  
Isolation And Purification ◽  
Ethanol Precipitation ◽  
Water Solvent

As an abundant marine xanthophyll, fucoxanthin (FX) exhibits a broad range of biological activities. The preparation of high-purity FX is in great demand, however, most of the available methods require organic solvents which cannot meet the green chemistry standard. In the present study, a simple and efficient purification approach for the purification of FX from the brown seaweed Sargassum horneri was carried out. The FX-rich ethanol extract was isolated by octadecylsilyl (ODS) column chromatography using ethanol–water solvent as a gradient eluent. The overwhelming majority of FX was successfully eluted by the ethanol–water mixture (9:1, v/v), with a recovery rate of 95.36%. A parametric study was performed to optimize the aqueous ethanol precipitation process by investigating the effects on the purity and recovery of FX. Under the optimal conditions, the purity of FX was 91.07%, and the recovery rate was 74.98%. Collectively, the eco-friendly method was cost-efficient for the purification of FX. The developed method provides a potential approach for the large-scale production of fucoxanthin from the brown seaweed Sargassum horneri.

scholarly journals Flow fields associated with in situ mineral leaching

1994 ◽  
Vol 36 (2) ◽  
pp. 133-151 ◽  
Author(s):  
Lawrence K. Forbes ◽  
Anthony M. Watts ◽  
Graeme A. Chandler
Keyword(s):  
Simple Model ◽  
Free Boundary ◽  
Numerical Method ◽  
Flow Rate ◽  
Recovery Rate ◽  
Flow Fields ◽  
Vertical Line ◽  
Rate Limiting ◽  
Mineral Leaching

AbstractA simple model for underground mineral leaching is considered, in which liquor is injected into the rock at one point and retrieved from the rock by being pumped out at another point. In its passage through the rock, the liquor dissolves some of the ore of interest, and this is therefore recovered in solution. When the injection and recovery points lie on a vertical line, the region of wetted rock forms an axi-symmetric plume, the surface of which is a free boundary. We present an accurate numerical method for the solution of the problem, and obtain estimates for the maximum possible recovery rate of the liquor, as a fraction of the injected flow rate. Limiting cases are discussed, and other geometries for fluid recovery are considered.

Research and Application of Multi-Slug and Equi-Fluidity Oil Displacement Mechanism

2015 ◽  
Vol 733 ◽  
pp. 43-46
Author(s):  
Jiang Min Zhao ◽  
Tian Ge Li
Keyword(s):  
Pressure Gradient ◽  
Flow Rate ◽  
Oil Recovery ◽  
Recovery Rate ◽  
Actual Situation ◽  
Displacement Efficiency ◽  
Displacement Method ◽  
Oil Displacement ◽  
Oil Displacement Efficiency ◽  
Swept Volume

In this paper, several aspects of the improvement of the oil recovery were analyzed theoretically based on the mechanism that equi-fluidity enhances the pressure gradient. These aspects include the increase of the flow rate and the recovery rate, of the swept volume, and of the oil displacement efficiency. Also, based on the actual situation, the author designed the oil displacement method with gathered energy equi-fluidity, realizing the expectation of enhancing oil recovery with multi-slug and equi-fluidity oil displacement method.

Hemoglobin A1C by Hplc with the Pharmacia Mono S HR 5/N Cation-Exchange Column: Influence of Sample Protein Load on Optimal Chromatographic Conditions

1992 ◽  
Vol 38 (8) ◽  
pp. 1488-1490 ◽  
Author(s):  
J C Philcox ◽  
M R Haywood ◽  
A M Rofe
Keyword(s):  
Cation Exchange ◽  
Flow Rate ◽  
Chromatographic Separation ◽  
Hemoglobin A1c ◽  
Analysis Time ◽  
Exchange Column ◽  
Cation Exchange Column ◽  
Protein Load

Abstract The Pharmacia Mono S HR 5/5 column has been optimized for hemoglobin A1c analysis by HPLC by using a much smaller column load, decreased buffer flow rate, and a steeper gradient than was used in previously described methods. Superior chromatographic separation, shorter analysis time, and a greatly extended column life have resulted.

Effects of Water Chemistry on the Leach Resistance of Synroc C

1983 ◽  
Vol 26 ◽  
Author(s):  
J. L. Woolfrey ◽  
D. M. Levins
Keyword(s):  
Water Chemistry ◽  
Flow Rate ◽  
Volume Ratio ◽  
Chemical Durability ◽  
Borosilicate Glasses ◽  
Solution Composition ◽  
Leach Rate ◽  
Saturation Effects ◽  
Ph Range ◽  
Temperature Surface

ABSTRACTThe chemical durability of SYNROC C has been measured as a function of repository conditions, namely, temperature, surface/volume ratio, groundwater pH and composition. The leach rate of SYNROC C is less dependent on temperature than borosilicate glasses; activation energies range from 15-25 kJ mol-1 compared to 33-74 kJ mol-1 for glass. The rate of release from SYNROC C is very low and independent of solution composition for the solutions studied. There is little variation in leach rate over the pH range 4-9, but at pH 2 the rate is up to 10 times higher. SYNROC C leach rate is independent of flow rate or surface/volume ratio for the mobile waste elements because of the unimportance of solution saturation effects owing to the low concentration of dissolved solids. The differential leach rate decreases rapidly with time because, after initial leaching from grain boundaries and metastable minor phases, the highly insoluble matrix protects the more leachable elements from further attack.

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