Study on Optimization of the Culture Conditions for Four Rhodopseudomonas spp.

2011 ◽  
Vol 393-395 ◽  
pp. 976-979
Author(s):  
Cheng Bin Xu ◽  
Xue Kai Sun ◽  
Yao Yao Li ◽  
Yan Gang Wang ◽  
Xue Lian Meng
Keyword(s):  
Wastewater Treatment ◽  
Orthogonal Experiment ◽  
Photosynthetic Bacterium ◽  
Culture Conditions ◽  
Qingdao Coast

Rhodopseudomonas sp. could be used in wastewater treatment and aquiculture, extensively. Four photosynthetic bacterium Rhodopseudomonas spp., QDS2, QDS3, QDS4 and QDS9, were isolated and screened from the bottom sludge of Qingdao coast. In order to get a mass of PSB, we successfully optimized the culture conditions of four strains by orthogonal experiment and obtained the best culture conditions. The research could provide the bases for the applying of high-effective PSB (Rhodopseudomonas sp).

The Best Culture Conditions of Rhodopseudomonas spp. by Orthogonal Experiment

2013 ◽  
Vol 800 ◽  
pp. 58-61
Author(s):  
Cheng Bin Xu ◽  
Yao Yao Li ◽  
Xue Lian Meng ◽  
Xue Kai Sun
Keyword(s):  
Orthogonal Experiment ◽  
Photosynthetic Bacterium ◽  
Culture Conditions ◽  
Qingdao Coast

Four photosynthetic bacteriumRhodopseudomonas spp., QDS2, QDS3, QDS4and QDS9, were isolated and screened from the bottom sludge of Qingdao coast. In order to get a mass of PSB, we successfully optimized the culture conditions of four strains by orthogonal experiment and obtained the best culture conditions.

scholarly journals Pigment Formations and Culture Conditions of Photosynthetic Bacterium, Rhodopseudomonas capsulatus

1974 ◽  
Vol 48 (2) ◽  
pp. 97-104 ◽  
Author(s):  
Osamu HIRAYAMA ◽  
Eiji ANDO ◽  
Katsumasa WAMORI ◽  
Namiko HARA
Keyword(s):  
Photosynthetic Bacterium ◽  
Culture Conditions

Study on Radioactive Organic Wastewater Treatment by Fenton Reagent

2014 ◽  
Vol 685 ◽  
pp. 457-462 ◽  
Author(s):  
Ying Hong Xiang ◽  
Yang Yang Zhong ◽  
Chun Chen
Keyword(s):  
Wastewater Treatment ◽  
Ph Value ◽  
Orthogonal Experiment ◽  
Influential Factors ◽  
Cod Removal ◽  
Influential Factor ◽  
Fenton Reagent ◽  
Radical Oxidation ◽  
Radioactive Wastewater ◽  
Organic Wastewater

The radioactive organics in wastewater were removed by Fenton reagent. The optimum conditions determined by single-factor test and orthogonal experiment are as following: [H2O2] =1.0mol·L-1, [Fe2+] =6.00mmol·L-1 and 30min at pH=4.0. Under these conditions, the COD removal efficiency was 81%. The dosage of Fe2+ was the most influential factor on organic wastewater COD removal by Fenton and the following influential factors were the reaction time, H2O2 dosage and pH value. In the process of radioactive wastewater treatment by Fenton, the effect of molten iron complexes flocculation was bigger than hydroxyl radical oxidation on COD removal.

scholarly journals Microalgae cultivation in wastewater effluent from tilapia culture pond for enhanced bioethanol production

2021 ◽  
Author(s):  
Prakash Bhuyar ◽  
Marlen Trejo ◽  
Natthawud Dussadee ◽  
Yuwalee Unpaprom ◽  
Rameshprabu Ramaraj ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Culture Conditions ◽  
Bioethanol Production ◽  
Nitrogen And Phosphorus ◽  
Treatment Processes ◽  
Aerobic Wastewater Treatment ◽  
Inorganic Substances ◽  
A Cell ◽  
Wastewater Treatment Effluent ◽  
Wastewater Generation

Abstract The large number of wastewaters are generated because of the various production processes. Vegetable and fish processing can be considered an important industry for wastewater generation. The essential method for completing this waste is to digest the organic matter using anaerobic digestion followed by aerobic wastewater treatment processes; however, wastewater from tilapia culture pond retains considerable quantities of inorganic substances, particularly nutrients like nitrogen and phosphorus. The optimal conditions for cultivating Chlorella vulgaris from wastewater treatment effluent from tilapia culture pond were investigated in this study. The appropriate conditions were found to be 10% initial stock suspension, 20 cm depth, and 12 days of culture conditions. C. vulgaris had an optical density of 0.649, a cell density of 17.68 × 105 cells/mL, and biomass of 0.376 ± 94.21 mg/L after cultivation. Discharged wastewater from the fishpond was utilized for the improved growth of microalgae and obtained biomass was used for bioethanol production. This study verified that fishpond wastewater is the best source of nutrients for algal mass production and biofuel applications.

Bioflocculant production by Haloplanus vescus and its application in acid brilliant scarlet yellow/red removal

2015 ◽  
Vol 73 (4) ◽  
pp. 707-715 ◽  
Author(s):  
Chun-Ying Zhong ◽  
Hong-Gao Chen ◽  
Gang Cao ◽  
Jun Wang ◽  
Jian-Gang Zhou
Keyword(s):  
Wastewater Treatment ◽  
Contact Time ◽  
Oxygen Demand ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Dye Wastewater ◽  
Salt Tolerant ◽  
Initial Ph ◽  
Flocculating Rate ◽  
Optimized Conditions

A novel bioflocculant MBF057 produced by a salt-tolerant Haloplanus vescus HW0579 was investigated in this study. The effects of culture conditions such as initial pH, inoculum size, and chemical oxygen demand (COD) of K-acid wastewater on MBF0579 production were studied. The result showed that 8.09 g/L purified MBF0579 was extracted with the following optimized conditions: 780 mg/L COD of K-acid wastewater as carbon source, inoculum size 12.5%, and initial pH 7.0. The biopolymer contained 78.6% polysaccharides and 21.1% proteins. The highest flocculating rate of 81.86 and 95.07% for the COD and chroma of acid brilliant scarlet gelb rot (yellow/red, GR) dye wastewater were achieved at a dosage of 150 mg/L, pH 2.0 and contact time 100 min. Overall, these findings indicate bioflocculation offers an effective alternative method of decreasing acid brilliant scarlet GR during dye wastewater treatment.

scholarly journals Novel Carotenoid-Based Biosensor for Simple Visual Detection of Arsenite: Characterization and Preliminary Evaluation for Environmental Application

2008 ◽  
Vol 74 (21) ◽  
pp. 6730-6738 ◽  
Author(s):  
Kazuyuki Yoshida ◽  
Koichi Inoue ◽  
Yuko Takahashi ◽  
Shunsaku Ueda ◽  
Katsuhiro Isoda ◽  
...  
Keyword(s):  
Color Change ◽  
Rhodopseudomonas Palustris ◽  
Photosynthetic Bacterium ◽  
Culture Conditions ◽  
Preliminary Evaluation ◽  
Dependent Manner ◽  
Environmental Application ◽  
Reverse Transcription Pcr ◽  
Groundwater Samples ◽  
Hue Angle

ABSTRACT A novel whole-cell arsenite biosensor was developed using the photosynthetic bacterium Rhodopseudomonas palustris no. 7 and characterized. A sensor plasmid containing the operator-promoter region of the ars operon and arsR gene from Escherichia coli and the crtI gene from R. palustris no. 7 was introduced into a blue-green mutant with crtI deleted, R. palustris no. 711. The biosensor changed color in response to arsenite, and the change was obvious to the naked eye after 24 h without further manipulation. Real-time reverse transcription-PCR showed that the crtI mRNA was induced 3-fold at 3 h and 2.5-fold at 6 h after addition of 50 μg/liter arsenite compared with the no-arsenite control, and consistent with this, the relative levels of lycopene and rhodopin also increased compared with the control. Colorimetric analysis of the bacteria showed that the hue angle had clearly shifted from green-yellow toward red in an arsenic dose-dependent manner at 24 h after arsenite addition. This obvious shift occurred irrespective of the culture conditions before arsenite was added, indicating that the color change of the biosensor is stable in water samples containing various concentrations of dissolved oxygen. Finally, assays using samples prepared in various types of mineral water indicated that this biosensor could be used to screen groundwater samples for the presence of arsenite in a variety of locations, even where electricity is not available.

scholarly journals Marine Actinobacteria Bioflocculant: A Storehouse of Unique Biotechnological Resources for Wastewater Treatment and Other Applications

2020 ◽  
Vol 10 (21) ◽  
pp. 7671
Author(s):  
Oluyemi Olatunji Awolusi ◽  
Adedeji Nelson Ademakinwa ◽  
Abidemi Ojo ◽  
Mariana Erasmus ◽  
Faizal Bux ◽  
...  
Keyword(s):  
Neural Network ◽  
Artificial Neural Network ◽  
Wastewater Treatment ◽  
Response Surface ◽  
Culture Conditions ◽  
Response Surface Model ◽  
Surface Model ◽  
Marine Actinobacteria ◽  
Marine Habitats ◽  
Anti Cancer

The bioactive compounds produced by actinobacteria have played a major role in antimicrobials, bioremediation, biofuels, enzymes, and anti-cancer activities. Biodegradable microbial flocculants have been produced by bacteria, algae, and fungi. Microbial bioflocculants have also attracted biotechnology importance over chemical flocculants as a result of degradability and environmentally friendly attributes they possess. Though, freshwater actinobacteria flocculants have been explored in bioflocculation. Yet, there is a paucity of information on the application of actinobacteria flocculants isolated from the marine environment. Similarly, marine habitats that supported the biodiversity of actinobacteria strains in the field of biotechnology have been underexplored in bioflocculation. Hence, this review reiterates the need to optimize culture conditions and other parameters that affect bioflocculant production by using a response surface model or artificial neural network.

Effect of Anode Size, Membrane and Membrane Size on Power Generation and Wastewater Treatment in Microbial Fuel Cells

2012 ◽  
Vol 164 ◽  
pp. 506-510
Author(s):  
Guang Yu Zhou ◽  
Yuichiro Yoshino ◽  
Takahiro Yamashita ◽  
Takahiro Sato ◽  
Yasunori Kawagoshi
Keyword(s):  
Wastewater Treatment ◽  
Fuel Cells ◽  
Microbial Fuel Cells ◽  
Removal Rate ◽  
Orthogonal Experiment ◽  
Coulombic Efficiency ◽  
Abiotic Factors ◽  
Anaerobic Sludge ◽  
Toc Removal ◽  
The Impact

Using wastewater as substrate, taking anaerobic sludge as inoculant, microbial fuel cells (MFCs) have emerged in recent years, which can generate electricity and accomplish wastewater treatment simultaneously. Based on the evaluation indexes of output voltage, coulombic efficiency, power density and TOC removal rate, three abiotic factors, anode size, membrane and membrane size, affecting MFC performance were investigated with an orthogonal experiment (L4 (23)). The results show that the impact order of factors through analyzing the value “R” was “anode size > membrane > membrane size”. The optimal set with these three factors for the performance of MFCs was big size anode, Naf-117 and big size membrane. Meanwhile, the high TOC removal rate (more than 90%) and high acetates consumed rate (100%) show the MFCs have strong ability of wastewater treatment. Cation exchange membrane Yumi-28 has compared ability of wastewater treatment and energy-production potential in MFC work.

scholarly journals Isolation, Identification, and Optimization of Culture Conditions of a Bioflocculant-Producing BacteriumBacillus megateriumSP1 and Its Application in Aquaculture Wastewater Treatment

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Liang Luo ◽  
Zhigang Zhao ◽  
Xiaoli Huang ◽  
Xue Du ◽  
Chang’an Wang ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Bacillus Megaterium ◽  
Oxygen Demand ◽  
Nitrogen Sources ◽  
Pond Water ◽  
Ammonia Nitrogen ◽  
Culture Conditions ◽  
Sequencing Analysis ◽  
Rdna Sequencing ◽  
Aquaculture Wastewater

A bioflocculant-producing bacterium,Bacillus megateriumSP1, was isolated from biofloc in pond water and identified by using both 16S rDNA sequencing analysis and a Biolog GEN III MicroStation System. The optimal carbon and nitrogen sources forBacillus megateriumSP1 were 20 g L−1of glucose and 0.5 g L−1of beef extract at 30°C and pH 7. The bioflocculant produced by strain SP1 under optimal culture conditions was applied into aquaculture wastewater treatment. The removal rates of chemical oxygen demand (COD), total ammonia nitrogen (TAN), and suspended solids (SS) in aquaculture wastewater reached 64, 63.61, and 83.8%, respectively. The volume of biofloc (FV) increased from 4.93 to 25.97 mL L−1. The addition ofBacillus megateriumSP1 in aquaculture wastewater could effectively improve aquaculture water quality, promote the formation of biofloc, and then form an efficient and healthy aquaculture model based on biofloc technology.

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