Interaction between Hydroxyapatite and Collagen
To study the interaction between hydroxyapatite (HAP) and collagen in bone, we researched the phenomenon of collagen biomineralization and self-assembly in viro by uv-vis spectra and circular dichroism (CD) spectra. The materials prepared by self-assembly collagen and collagen-HAP showed layer structures. And the product prepared by collagen-HAP had better and more compact appearance. The decrease of speed of collagen self-assembly was caused by calcium ion or strontium ion added. The trough of CD spectra moved down in calcium-containing solution and moved up when forming precipitation of calcium phosphate from the solution. It indicated that the effect of collagen self-assembly was caused by calcium ions, strontium ions etc. in the solution. The IR spectrum proved that a coordinate bond formed between calcium ion and amide groups on collagen.