Characterization of Eugenol Extracted from Lemongrass (Cymbopogon citratus (DC.) Stapf) for Food Packaging Materials

2012 ◽  
Vol 506 ◽  
pp. 603-606 ◽  
Author(s):  
Natchanok Petchsoongsakul ◽  
Chiravoot Pechyen
Keyword(s):  
Steady State ◽  
Free Radical ◽  
Food Packaging ◽  
Raw Materials ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Property ◽  
Butylated Hydroxytoluene ◽  
Cymbopogon Citratus ◽  
Antioxidant Agent

The goal of this research was to study a prospect of applying Lemongrass (Cymbopogon citratus (DC.) Stapf) extracted having antioxidant property (i.e. eugenol) to replace commercial antioxidant agent such as butylated hydroxytoluene (BHT) in packaging raw materials. The extracted eugenol was characterized using Gas chromatography (GC). A free radical scavenging activities of eugenol and BHT were also investigated using 2, 2-dipheny l-1-picryhydrazyl (DPPH) assay. It reveals that a concentration of eugenol resulting in a 50% inhibition of the free radical, IC50, (0.11 mg/ml) is lower than the IC50 value of BHT (0.14 mg/ml) indicating better radical scavenging activity. In its radical form, DPPH shows an absorbance maximum at 515 nm which disappears upon reduction by an antiradical compound. BHT, a synthetic antioxidant, slowly reacts with DPPH reaching a steady state within 5 hr. The kinetic (R2) is estimated to be 0.9283 at 25 °C. Eugenol rapid reacts with DPPH reaching a steady state within 2 h. The kinetic (R2) is estimated to be 0.9946 at 25 °C, our results conrm that eugenol can we used instead of BHT.

scholarly journals Lipid Lowering Activity and Free Radical Scavenging Effect of Cinnamomum tamala (Fam: Lauraceae)

1970 ◽  
Vol 1 (4) ◽  
pp. 93-96
Author(s):  
R Al-Mamun ◽  
A Hamid ◽  
MK Islam ◽  
JA Chowdhury ◽  
ATM Zafrul Azam
Keyword(s):  
Free Radical ◽  
Lipid Profile ◽  
Total Cholesterol ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Property ◽  
Free Radical Scavenging ◽  
Lipid Lowering ◽  
Increased Risk ◽  
Lowering Activity

Lipid profile is a blood test that measures the amount of lipids, or fats, in your blood. The lipids measured are usually total cholesterol, HDL-C, LDL-C and triglycerides. When levels of these lipids are abnormal, there is an increased risk of heart attack and stroke. In our study, we use methanol crude extract for lipid lowering activity on rabbit and found that lipid profile was reduced by 14.0, 1.0, 4.0 and 15.0 mg/dl for total cholesterol, HDL-C, LDL-C and triglyceride respectively after using the plant extract (dose 500mg/rabbit for 10 days); where atorvastatin (0.005mg/rabbit) was used as standard lipid lowering agent. We also focused on the antioxidant property of crude methanol extract. Here we also carried out free radical-scavenging activity study and found the IC50 value for C. tamala is 6.00 μg/ml where the standard antioxidant (ascorbic acid) gave the value of 3.21μg/ml. DOI: http://dx.doi.org/10.3329/ijns.v1i4.9735 IJNS 2011 1(4): 93-96

Antimicrobial and Antioxidant Properties of Fruits of Capsicum annuum var. cerasiforme (Mill.) Irish and Heracleum nepalense D. Don

2017 ◽  
Vol 9 (02) ◽  
Author(s):  
Koirala Pramila ◽  
Singh Bimala
Keyword(s):  
Escherichia Coli ◽  
Free Radical ◽  
Capsicum Annuum ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Property ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
The Family

The fruit of Capsicum annuum var. cerasiforme belonging to the Family Solanaceae is a widely used spice in Sikkim, a North Eastern state of India. The fruit of Capsicum annuum var. cerasiforme locally called ‘Dalle Khorsani’ is a valuable cash crop of Sikkim and also possess medicinal properties. Heracleum nepalense D. Don locally called ‘Chimphing’ belongsto the Family Umbillifereae and is extensively used in folk medicine. The present study evaluated the antimicrobial property of fruits of Capsicum annuum var. cerasiforme (Mill.) Irish and Heracleum nepalense D. Don using agar well diffusion method against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus vulgaris, Staphylococcus aureus, Bacillus cereus and Bacillus subtilis. The antioxidant property of methanol extracts of fruits of these test plants was also investigated. The antioxidant property was evaluated by 2, 2-diphenyl-1-picryl hydrazyl (DPPH) free radical scavenging activity assay and Ferric reducing power assay. The total phenolic content was determined by using Gallic acid as a standard. The methanol extract of both the plants inhibited the growth of mostly Gram positive bacteria and Escherichia coli among Gram negative bacteria. However the acetone extract of the selected plants did not exhibit antimicrobial activity. The methanol extracts of these plants exhibited significant (p>0.05) concentration dependent increase in DPPH free radical scavenging activity. The extract of Capsicum annuum var. cerasiforme (Mill.) Irish also exhibited significantly higher (p>0.05) ferric reducing ability thereby showing higher antioxidant activity. The phytochemical analyses of the plant extracts revealed the presence of phenol, tannin, flavonoid, alkaloid, steroid and saponin. The results of the present study indicate that Capsicum annuum var. cerasiforme (Mill.) Irish and Heracleum nepalense D. Don can be a potential source of antimicrobial and antioxidant agents and can be explored further for its therapeutic use.

Study on the Antioxidant Activities of Gel Containing Tagetes erecta (L.)

2013 ◽  
Vol 747 ◽  
pp. 741-744 ◽  
Author(s):  
Tunyaluk Limsuwan ◽  
Prapaporn Boonme ◽  
Thanaporn Amnuaikit
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Common Garden ◽  
Butylated Hydroxytoluene ◽  
Gelling Agent ◽  
Tagetes Erecta ◽  
Radical Scavenging Properties

Tagetes erecta Linn. (Asteraceae), commonly known as marigold, is a common garden plant in Thailand. It was reported that antioxidant, antimycotic, and analgesic activities were found in Tagetes erecta. In this study, the alcoholic crude extracts of flowers of Tagetes erecta were evaluated its free radical scavenging properties by using 2,2diphenyl-1-picrylhydrazyl (DPPH) free radical. Butylated hydroxytoluene (BHT) was used as standard antioxidant. Tagetes erecta extract possessed the potency of DPPH radical scavenging activity (EC50 6.56±1.46 μg/ml) more than BHT (EC50 11.06±1.75 μg/ml). Later, it was prepared in gel formulation at concentration of 0.1 % w/w Tagetes erecta extract which Carbopol Ultrez 21 or Sepigel 305 was used as a gelling agent. Propylene glycol, Uniphen P-23, triethanolamine, and distilled water were used as ingredients. The physicochemical property was evaluated in terms of physical appearances, pH, viscosity, and antioxidant activity. All formulations possessed good antioxidant activity. In addition, it has good physical appearances, and pH ranged of 4 to 6. However, the viscosity depended on a polymer type and concentration of polymer.

scholarly journals Ilex khasiana - The silent holly species of Aquifoliaceae on its pharmacognostical importance as a free radical scavenger and antibacterial agent

2020 ◽  
Vol 11 (2) ◽  
pp. 2438-2444
Author(s):  
Charles Lalnunfela ◽  
Lalremsanga H.T. ◽  
Lalhriatpuii T.C. ◽  
Lalthanpuii P.B. ◽  
Kholhring Lalchhandama
Keyword(s):  
Free Radical ◽  
Medicinal Plant ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Northeast India ◽  
Free Radical Scavenger ◽  
Butylated Hydroxytoluene ◽  
Radical Scavenger ◽  
Endangered Medicinal Plant ◽  
Rare And Endangered

Ilex khasiana is a rare and endangered medicinal plant endemic to northeast India. Remaining largely unexplored,it is purported to have a range of medicinal values and may soon join the medicinal plant hall of fame.Extracts of the leaves were prepared using solvents of increasing polarity, namelypetroleum ether (IKP), chloroform (IKC) and methanol (IKM).The preliminary phytochemical screening indicated the presence of alkaloids, phytosterols, triterpenoids, saponins, reducing sugars, glycosides and carbohydrates. Free radical-scavenging activity was assessed using2,2-diphenyl-1-picrylhydrazyl(DPPH). IKC exhibited the highest activity with an IC50 of 17.22 ± 1.87, followed by IKM with an IC50 of 26.93 ± 5.14 and IKP with an IC50 of 37.16 ± 5.11. Butylated hydroxytoluene (BHT)was used as the standard DPPH scavenger and showed an IC50 of 8.31 ±0.72. IKM and IKC also showed positive antibacterial activity againstGram-negative bacteria such asEscherichia coli, Pseudomonas aeruginosa, andKlebsiella pneumoniae, and a Gram-positive bacteriaBacillus subtilis. IKP did not indicate any inhibition against the selected bacteria. Our findings substantiate the basis for further investigations on the medicinal potentials of the plant.  

scholarly journals Antimicrobial and antioxidant property of Curcuma longa Linn.

2019 ◽  
Vol 8 (11) ◽  
pp. 2383 ◽  
Author(s):  
Intan Athirah Muhamed ◽  
Wan Amir Nizam Wan Ahmad ◽  
Nurul Syahida Ramli ◽  
Noraini A. Ghafar
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Curcuma Longa ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Antioxidant Property ◽  
Butylated Hydroxytoluene ◽  
Dpph Radical ◽  
Dpph Radical Scavenging

Background: Curcuma longa Linn. (Zingiberaceae family) plant or turmeric is known to possess pharmacological properties at relatively nontoxic doses. The study was carried out with the aim to evaluate the antimicrobial and antioxidant activities of C. longa L.Methods: Ethanolic rhizome extract was applied against Staphylococcus aureus and Salmonella typhi using disc and well diffusion methods. Antioxidant of C. longa L. was determined by measuring 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity.Results: The ethanolic extract of rhizomes showed inhibitory activity against Gram-positive S. aureus bacteria, whereas the Gram-negative S. typhi bacteria were resistant toward the ethanol extract. Zone of inhibitions against S. aureus were 7 mm and 11 mm by disc and well method, respectively. For antioxidant activity, DPPH radical scavenging activity of ethanolic extract of C. longa L. was set against butylated hydroxytoluene (BHT). It was shown that the value of half maximal inhibitory concentration (IC50) of rhizome extract was higher than standard control. The dose response curve was utilized to determine the half maximal IC50 value for each sample. The IC50 of rhizome extract is 21.25 µg/ml, while the value IC50 for BHT is 12.42 µg/ml.Conclusions: The finding suggested turmeric rhizome-extract as a good candidate in the search for a natural antimicrobial and antioxidant agent.

scholarly journals Pharmacognostical standardization, phytochemical analysis, and antioxidant activity of Musa balbisiana Colla fruits

2020 ◽  
Vol 11 (4) ◽  
pp. 7920-7931
Author(s):  
Ly Hai Trieu ◽  
Le My Huyen ◽  
Lam Bich Thao ◽  
Le Duc Thanh ◽  
Pham Thanh Huyen ◽  
...  
Keyword(s):  
Crude Extract ◽  
Raw Materials ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dry Weight ◽  
Antioxidant Property ◽  
Phytochemical Analysis ◽  
Musa Balbisiana

Musa balbisiana (MB) fruits are widespread as an herbal medicine worldwide.  This study aimed to screen phytochemicals, standardize raw materials and crude extract, and evaluate antioxidant property of MB fruit extracts. Standardization was performed using purity content and chemical characterizations, total polyphenol (TPC) and flavonoid (TFC) contents to assess the quality. The phytochemicals were determined by chemical reactions. DPPH quenching, ABTS cation decolorization, and reducing power assays were selected to investigate antioxidant ability. Results indicated that physicochemical parameters for MB fruits were within prescribed Vietnamese Pharmacopoeia 5th limits.  Phytochemical analysis of MB fruits disclosed the presence of volatile oils, lipids, flavonoids, proanthocyanidins, saponins, and triterpenoid. The TPC was 3.92 ± 0.27 mg GAE/g d. w. (dry weight) and 162.64 ± 3.39 mg GAE/g d. w. for the raw materials and crude extract respectively while the TFC was 0.138 ± 0.002 mg QE/g d. w. and 1.222 ± 0.007 mg QE/g d. w. Furthermore, crude extract and some of its fractions have a high radical scavenging activity. These initial studies play a pivotal role in the procurement of raw materials and evaluation of the quality of the crude extract, and show antioxidant potential of MB fruits. 

Antioxidant and Free Radical Scavenging Activities of N-Modified Chitosans

2014 ◽  
Vol 1002 ◽  
pp. 91-98
Author(s):  
Qing Xu ◽  
Tian Zhong ◽  
Hui Li Li
Keyword(s):  
Free Radical ◽  
Nitrogen Atom ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Antioxidant Property ◽  
Free Radical Scavenging ◽  
Biomedical Science ◽  
Modified Chitosan ◽  
Free Radical Scavenging Activities

Recently, the search for effective natural antioxidants use in food and medicinal fields to replace synthetic ones has attracted the most attention. Chitosan, a natural nontoxic biopolymer, is known to possess antioxidant property, which is attributed that NH2 and OH attached in the pyranose ring react with unstable free radicals to form stable macromolecule radicals. It has been observed that the antioxidant activity of chitosan is closely related to the form of nitrogen atom in the molecules. This review focuses on the antioxidant and free radical scavenging activities of various nitrogen atom of chitosan, including N-acylated chitosans, Schiff bases of chitosan, N-alkyl chitosan, and N-quaternized chitosan. The free radical scavenging mechanisms are summarized and the effects on free radical scavenging activity of N-modified chitosan are discussed. This work may provide more insights into the antioxidant ability of N-modified chitosans and potentially enable them to be used as natural antioxidants for application in food, agriculture, cosmetic and biomedical science. Keywords: N-modified chitosan, Sythesis, Antioxidant, Free radical scavenging

scholarly journals Free radical scavenging activity of Maranta arundinacea assisted selenium nanoparticles

2020 ◽  
Vol 11 (3) ◽  
pp. 4214-4217
Author(s):  
Apurva Choudhary ◽  
Rajeshkumar S ◽  
Anita Roy ◽  
Lakshmi T
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Property ◽  
Free Radical Scavenging ◽  
Selenium Nanoparticles ◽  
Free Radical Scavenging Activity ◽  
Toxic Waste ◽  
Scavenging Activity ◽  
Arrow Root

Selenium nanoparticles have role in toxic waste removal and bioremediation. In a recent study, biosynthesis of many metal and metal oxide nanoparticles by different plant extracts was found to be stable even after wet heat sterilization process. In some studies, biosynthesis of silver, gold, and selenium nanoparticles using microorganisms and plant and parts. In this present study we have synthesized selenium nanoparticles using arrow root and analysed for its free radical scavenging activity. 20 mM of sodium selenite was prepared using 60 mL of distilled water and 40 mL of Arrowroot extract. It was kept in the shaker. Readings were taken for 3 days. Centrifugation was done at 8000 rpm. Pellet was collected and was used for antioxidants activity that is free radical scavenging activity. The antioxidant property of arrow root mediated selenium nanoparticles are comparatively higher than the standard antioxidant property. Calculations were done on the basis of reading that was obtained. This study proves that there is antioxidant activity that is free radical scavenging activity adopted by selenium nanoparticles extracted from arrow root.

Sign in / Sign up

Export Citation Format

Share Document