Optimization of Sargassum Extracts Preparation with Complex Enzyme by Response Surface Methodology

2012 ◽  
Vol 550-553 ◽  
pp. 1653-1658
Author(s):  
Shu Xing Liu ◽  
Shuang Zhang ◽  
Wei Na Jiao
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Sugar Content ◽  
Reducing Sugar ◽  
Enzyme Hydrolysis ◽  
Hydrolysis Time ◽  
Design Expert ◽  
Preparation Conditions ◽  
Hydrolysis Temperature ◽  
Good Repeatability

Use Design Expert software to establish a quadratic polynomial model between four factors and content of reducing sugar which is characterization for hydrolysis. The four factors are the amount of complex enzyme, the ratio of complex enzyme, hydrolysis temperature and hydrolysis time. Process of sargassum extracts preparation with complex enzyme is optimized by response surface methodology. The optimum preparation conditions were achieved as follows：amount of complex enzyme is 2.05%, ratio of complex enzyme is 1.98, hydrolysis temperature is 55°C, hydrolysis time is 64minutes, and the final reducing sugar content is 0.832% which is good repeatability.

Optimization of L-Arabinose Hydrolization from Corn Fibers Using Response Surface Methodology

2011 ◽  
Vol 396-398 ◽  
pp. 1662-1666
Author(s):  
Ze Sheng Zhang ◽  
Min Zheng ◽  
Hong Fei Zou
Keyword(s):  
Response Surface Methodology ◽  
Oxalic Acid ◽  
Response Surface ◽  
Reaction Temperature ◽  
Coefficient Of Determination ◽  
Hydrolysis Time ◽  
Design Expert ◽  
Independent Variables ◽  
Significant Regression ◽  
Corn Fibers

The main objective of this study was to optimize the hydrolyzing conditions of L-Arabinose from corn fibers. The response surface methodology was used for the optimization. Independent variables were the concentration of the oxalic acid (2-4%), the hydrolysis time (3.5-4.5h) ,the dosage of the oxalic acid(6-10ml/g,v/w)and the reaction temperature (80-100°C). Significant regression model describing the changes of L-Arabinose yield with respect to hydrolysis parameters were established with the coefficient of determination, R2= 0.9501.Data were analyzed by Design Expert 7.0 and regression analysis. The L-Arabinose yield ranged from 7.53% to 11.46%. The models had significant effects on L-Arabinose yield at P<0.05. Optimum the hydrolysis time, the concentration of the oxalic acid, the dosage of the oxalic acid and the reaction temperature were 3.86h, 3.97%, 6mL/g (v/w) and 100°C, respectively. This combination gave 11.46% L-Arabinose yield.

scholarly journals Optimization of Oyster (Crassostrea talienwhanensis) Protein Hydrolysates Using Response Surface Methodology

Molecules ◽  
2020 ◽  
Vol 25 (12) ◽  
pp. 2844 ◽  
Author(s):  
Xueqin Wang ◽  
Huahua Yu ◽  
Ronge Xing ◽  
Song Liu ◽  
Xiaolin Chen ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Enzyme Concentration ◽  
Degree Of Hydrolysis ◽  
Hydrolysis Time ◽  
Hydrolysis Temperature ◽  
Hydrolysis Conditions ◽  
Different Response

Oyster (Crassostrea talienwhanensis) protein was hydrolyzed by trypsin to produce peptides with different response values, and response surface methodology (RSM) was applied to optimize the hydrolysis conditions. The highest degree of hydrolysis (DH) of the oyster peptide (OP) was obtained at an enzyme concentration of 1593.2 U/g, a pH of 8.2, a hydrolysis temperature of 40.1 °C, a hydrolysis time of 6.0 h, and a water/material ratio of 8.2. The greatest hydroxyl-radical-scavenging activity of OP was obtained at an enzyme concentration of 1546.3 U/g, a pH of 9.0, a hydrolysis temperature of 50.2 °C, a hydrolysis time of 5.1 h, and a water/material ratio of 5.6. The largest branched-chain amino acid (BCAA) content of OP was obtained at an enzyme concentration of 1323.8 U/g, a pH of 8.3, a hydrolysis temperature of 41.7 °C, a hydrolysis time of 6.7 h, and a water/material ratio of 4.8. The three experimental values were significantly in agreement with the predicted values within the 95% confidence interval. Furthermore, ultrafiltration and chromatographic methods were used to purify the OP, and 13 peptides that were rich in Lys, Arg, His, and Thr were identified by LC-MS/MS. The results of this study offer different optimum hydrolysis conditions to produce target peptides from oyster protein by using RSM, and this study provide a theoretical basis for the high-value utilization of oyster protein.

scholarly journals Assessment of TiO2 Nanoconcentration and Twin Impingement Jet of Heat Transfer Enhancement—A Statistical Approach Using Response Surface Methodology

Energies ◽  
2021 ◽  
Vol 14 (3) ◽  
pp. 595
Author(s):  
Mahir Faris Abdullah ◽  
Rozli Zulkifli ◽  
Hazim Moria ◽  
Asmaa Soheil Najm ◽  
Zambri Harun ◽  
...  
Keyword(s):  
Heat Transfer ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Heat Transfer Rate ◽  
Transfer Rate ◽  
Tio2 Nanoparticle ◽  
Experimental Results ◽  
Improvement Rate ◽  
Design Expert ◽  
Impingement Jet

Impinging jets are considered to be a well-known technique that offers high local heat transfer rates. No correlation could be established in the literature between the significant parameters and the Nusselt number, and investigation of the interactions between the correlated factors has not been conducted before. An experimental analysis based on the twin impingement jet mechanism was achieved to study the heat transfer rate pertaining to the surface plate. In the current paper, four influential parameters were studied: the spacing between nozzles, velocity, concentration of Nano solution coating and nozzle-plate distance, which are considered to be effective parameters for the thermal conductivity and the heat transfer coefficient of TiO2 nanoparticle, an X-ray diffraction (XRD) and field emission scanning electron microscopy (FESEM) analysis were done, which highlighted the structure and showed that the nanosolution coated the surface homogenously. Moreover, a comparison was done for the experimental results with that of the predicted responses generated by the Design Expert software, Version 7 User’s Guide, USA. A response surface methodology (RSM) was employed to improve a mathematical model by accounting for a D-optimal design. In addition, the analysis of variance (ANOVA) was employed for testing the significance of the models. The maximum Nu of 91.47, where H = S = 1 cm; Reynolds number of 17,000, and TiO2 nanoparticle concentration of 0.5% M. The highest improvement rate in Nusselt was about 26%, achieved with TiO2 Nanoparticle, when S = 3 cm, H = 6 cm and TiO2 nanoparticle = 0.5 M. Furthermore, based on the statistical analysis, the expected values were found to be in satisfactory agreement with that of the empirical data, which was conducted by accounting for the proposed models’ excellent predictability. Multivariate approaches are very useful for researchers, as well as for applications in industrial processes, as they lead to increased efficiency and reduced costs, so the presented results of this work could encourage the overall uses of multivariate methods in these fields. Hypotheses: A comparison was done for the predicted responses generated by the Design Expert software with the experimental results and then studied to verify the following hypotheses: ► Preparation of three concentrations of TiO2 nanosolution was done and studied. ► The heat transfer rate could be increased by surface coating with TiO2 nanoparticle. ► The heat transfer could be improved by the impingement jet technique with suitable adjustments.

Preparation of Activated Carbon from Formic Acid Hydrolysis Residue by Chemical Activation of ZnCl2

2013 ◽  
Vol 860-863 ◽  
pp. 527-533
Author(s):  
Zhen Wu ◽  
Yong Sun ◽  
Lei Hu ◽  
Ning Xu ◽  
Ben Lin Dai
Keyword(s):  
Response Surface Methodology ◽  
Activated Carbon ◽  
Formic Acid ◽  
Acid Hydrolysis ◽  
Wheat Straw ◽  
Response Surface ◽  
Reaction Temperature ◽  
Preparation Conditions ◽  
Formic Acid Hydrolysis ◽  
Hydrolysis Residue

Utilization of wheat straw for bio-based chemicals production is a research focus. In this work, experiments were conducted to study the preparation conditions of activated carbon from formic acid hydrolysis residue of wheat straw applying response surface methodology. The effects of activation reaction temperature, retention time and activator quantity on the decolorizing capacity of activated carbon were dealt with in this paper. Optimal preparation conditions were abtained by response surface methodology as followed: the content of ZnCl2 solution was 14.2%, reaction temperature was 798°C and retained time was 30 mins with a decolorizing capacity of 15.8 mL methylene blue. Results indicated that the technology was available.

Optimization of Surface Grinding Process Parameters for Minimum Surface Roughness in AISI 1080 Using Response Surface Methodology

2014 ◽  
Vol 984-985 ◽  
pp. 118-123 ◽  
Author(s):  
S. Periyasamy ◽  
M. Aravind ◽  
D. Vivek ◽  
K.S. Amirthagadeswaran
Keyword(s):  
Surface Roughness ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Process Parameters ◽  
Surface Grinding ◽  
Steel Plates ◽  
Medium Effect ◽  
Depth Of Cut ◽  
Design Expert ◽  
Minimum Surface

In this study, the response surface methodology was used to optimize the process parameters of constant speed horizontal spindle surface grinding. The experiments were conducted based on the design expert software. The surface roughness characteristics were investigated in AISI 1080 steel plates using A60V5V grinding wheels. The optimum parameters for minimum surface roughness were found using Design Expert software. The parameters for a particular surface roughness value can also be determined using the results of this experiment. This results shows that feed has a greater effect on surface roughness and feed has medium effect on surface roughness. While dressing depth of cut has a very minimal effect on surface roughness.

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