Study on Compositional Change of Berchemia lineata from Different Harvest Times
To study content of quercetin in Zhuang folk medicine Berchemia lineata in the different harvest times and to explore the laws of dynamic changes of quercetin content. Methods: determine 10 batches of Berchemia lineata in the HPLC-DAD method. Chromatographic conditions: Agilent XDB C18(150 mm×4.6 mm, 5 μm), the column temperature of 25 °C; mobile phase of methanol (A) - acetonitrile (B) -0.05% phosphoric acid solution (C), gradient elution; volume flow of 1 mL/min, detection wavelength of 254 nm. Result: sample injection volume of quercetin in the range of 0.69 μg ~ 4.83 μg has a good linear relationship with peak area, the average recovery is 99.8%, RSD: 1.27% (n = 6). From the overall analysis, content of quercetin in Berchemia lineata harvested in January is higher than that in other months. Conclusion: January should be the best month for harvest of Berchemia lineata.