Optimization of Extraction Process of Water-Soluble Polysaccharides from Porphyra by Response Surface Methodology

2013 ◽  
Vol 864-867 ◽  
pp. 526-530
Author(s):  
Ying Chang Li ◽  
Ya Li Wang ◽  
Zuo Wei Li
Keyword(s):  
Response Surface Methodology ◽  
Sulfuric Acid ◽  
Response Surface ◽  
Industrial Process ◽  
Extraction Process ◽  
Water Soluble ◽  
Hot Water ◽  
Social Benefits ◽  
Process Conditions ◽  
Extraction Temperature

Polysaccharide from Porphyra is a kind of important biological active substance. Therefore，it is significant to effectively develop and utilize the polysaccharide from porphyra and improve the economic and social benefits of porphyra. In this paper, polysaccharide was extracted by the method of hot water immersed extraction and ethanol precipitation. Extraction process of water-soluble polysaccharides from Porphyra was optimized by response surface methodology. The content of polysaccharide was determined by sulfuric acid to phenol method. The results show that the extraction time is 4.4 h, extraction temperature is 88°Cand the ratio of material to liquid is1:42(g/mL). Polysaccharide is precipitated with 5 folds volume of ethanol for 6 h. The proportion of the extraction of the porphyra polysaccharide under this industrial process conditions is 10.012%.

scholarly journals Optimization of aqueous extraction conditions for bioactive compounds from fresh Pouzolzia zeylanica plant using response surface methodology

2020 ◽  
Vol 19 (03) ◽  
pp. 65-74
Author(s):  
Tan D. Nguyen
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Extraction Process ◽  
Hot Water ◽  
Soluble Solids ◽  
Extraction Temperature ◽  
Total Soluble Solids ◽  
Dependent Variables ◽  
Solids Content ◽  
Experimental Values

Response surface methodology was applied to optimize the extraction of phenolic compounds from fresh Pouzolzia zeylanica plant using hot water as a solvent. A central composite design (CCD) in form (23+star) was used to investigate the effects of two independent variables, namely, extraction temperature (70 to 90oC) and extraction time (20 to 40 min). The dependent variables were the content of anthocyanin, flavonoid, polyphenol, tannin and total soluble solids of extracted solution. A second-order polynomial model was used for predicting the response. The results showed that the optimal extraction process was obtained at 84.4oC for 31.7 min. The experimental values agreed with predicted within a 95% confidence interval. Consequently, the contents of anthocyanin, flavonoid, polyphenol and tannin were 38.66 mgCE/100 g, 3.01 mgQE/g, 5.17 mgGAE/g, 4.07 mgTAE/g fresh weight, and total soluble solids content was 0.73%, respectively.

scholarly journals Homogenate Extraction of Crocins from Saffron Optimized by Response Surface Methodology

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Yingpeng Tong ◽  
Yu Jiang ◽  
Dan Guo ◽  
Yongqiu Yan ◽  
Shiping Jiang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Extraction Time ◽  
Process Conditions ◽  
Extraction Temperature ◽  
Single Factor ◽  
Extraction Voltage ◽  
Homogenate Extraction

Saffron, which has many kinds of biological activities, has been widely used in medicine, cosmetics, food, and other fields of health promotion industries. Crocins are the main component of saffron (Crocus sativus L.). At present, most of the extraction methods for crocins require long time or special instruments to complete the process and some of them are not suitable for industrial production at present. In this article, homogenate extraction technology which is a convenient and efficient method was developed for crocins extraction from saffron. Firstly, the influences of extraction voltage, extraction time, ethanol concentration, and temperature on crocins yield were studied by single factor experiments; and then response surface methodology (RSM) was used to optimize levels of four variables based on the result of single factor experiments. Results showed that the optimum extraction process conditions for crocins were as follows: extraction voltage, 110 V; ethanol concentration, 70%; extraction temperature, 57°C; and extraction time, 40 s. Based on these conditions, the extraction yield of crocins can reach 22.76% which is higher than ultrasonic extraction method. Therefore, homogenate extraction is an effective way to extract crocins from saffron with higher extraction yield and shorter extraction time.

Optimization of aqueous extraction of orevactaene and flavanoid pigments produced by Epicoccum nigrum

2019 ◽  
Vol 48 (4) ◽  
pp. 301-308 ◽  
Author(s):  
Sawinder Kaur ◽  
Paramjit S. Panesar ◽  
Sushma Gurumayum ◽  
Prasad Rasane ◽  
Vikas Kumar
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Maximum Yield ◽  
Polynomial Equation ◽  
Extraction Process ◽  
Aqueous Extraction ◽  
Extraction Temperature ◽  
Content Type ◽  
Epicoccum Nigrum ◽  
Broken Rice

Purpose The extraction of bioactive compounds such as pigments from natural sources, using different solvents, is a vital downstream process. The present study aims to investigate the effect of different variables, namely, extraction temperature, mass of fermented rice and time on the extraction process of orevactaene and flavanoid pigment from Epicoccum nigrum fermented broken rice. Design/methodology/approach Central composite rotatable design under response surface methodology was used for deducing optimized conditions. The pigments were extracted under conditions of extraction temperature (40-70°C), mass of fermented rice (0.5-1.5 g) and time (30-90 min), using water as the extraction media. The experimental data obtained were studied by analysis of variance. Data were fitted to a second-order polynomial equation using multiple regression analysis. Findings The optimum conditions generated by the software for aqueous extraction process, i.e. extraction temperature of 55.7°C, 0.79 g of fermented matter and extraction time of 56.6 min, resulted in a pigment yield of 52.7AU/g orevactaene and 77.2 AU/g flavanoid. Research limitations/implications The developed polynomial empirical model for the optimal recovery of the orevactaene and flavanoid pigments could be used for further studies in prediction of yield under specified variable conditions. Practical implications The response surface methodology helped in optimizng the conditions for the eco-friendly low-cost aqueous extarction process for orevactaene and flavanoid pigments, produced by Epicoccum nigrum during solid state fermentation of broken rice. This optimization can provide the basis for scaling up for industrial extraction process. Originality/value This paper focuses on optimizing the extraction conditions to get the maximum yield of orevactaene and flavanoid pigments, using water as the extracting media. No literature is available on the optimization of the extraction process of Epicoccum nigrum pigments, to the best of the authors’ knowledge.

Optimization of Extraction Process of Lycopene from Watermelon (Citrullus lanatus) by Response Surface Methodology

2011 ◽  
Vol 140 ◽  
pp. 385-393 ◽  
Author(s):  
Xian Feng Shi ◽  
Yuan Xu ◽  
Yu Hua Li ◽  
Hong Xia Zeng ◽  
Yu Hong Sun
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Citrullus Lanatus ◽  
Extraction Process ◽  
Quadratic Equation ◽  
Raw Material ◽  
Extraction Time ◽  
Solvent Ratio ◽  
Extraction Temperature ◽  
Extraction Solvent

The extraction processing of lycopene from watermelon was investigated in this paper. Based on single factor experiments, response surface methodology (RSM) was adopted to study the key parameters such as number of extraction, extraction temperature, extraction time in order to increase extraction rate of lycopene. A second order quadratic equation was established and the applicability of model and interaction involved factors on predicting the lycopene extracting content was verified. The results indicated that the optimum extraction conditions were hexane (containing 2% dichloromethane) as extraction solvent, ratio of solvent to raw material of 3:1 (mL/g), number of extraction of 2, extraction time of 1.9 h and extraction temperature of 29.8°C. Under these conditions the extracting content of lycopene was 14.71±0.22 mg/kg, which is well in agreement with value predicted by the model.

scholarly journals Optimization of process conditions for microwave-assisted flax water retting by response surface methodology and evaluation of its fiber properties

BioResources ◽  
2020 ◽  
Vol 15 (3) ◽  
pp. 5859-5870
Author(s):  
Dan Zhao ◽  
Hairui Ji ◽  
Renpeng Du ◽  
Qi Wang ◽  
Wenxiang Ping ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Tensile Properties ◽  
Response Surface ◽  
Process Parameters ◽  
Microwave Power ◽  
Hot Water ◽  
Optimum Process ◽  
Process Conditions ◽  
Microwave Assisted ◽  
Microwave Assistance

Microwave-assistance was used to increase the degumming efficiency in flax water retting. Different pre-soaking times, microwave times, and microwave power were investigated in this study. The relationships between degumming rate and process parameters were established via response surface methodology (RSM). The optimum process parameters were a pre-soaking time of 25.5 h, a microwave time of 18.5 min, and a microwave power setting of 570 W. Under these optimal conditions, the degumming rate was 83.85% ± 1.13%, which was 1.33 times higher than that of natural hot water retting (P < 0.05). Moreover, the tensile properties and color of the resulting fibers showed that they had tensile properties similar to those of the natural hot water retting fibers. However, the color values for the natural hot water retting fibers were higher than those of the fibers treated with microwave-assisted flax water retting.

scholarly journals Optimization of Baicalin, Wogonoside, and Chlorogenic Acid Water Extraction Process from the Roots of Scutellariae Radix and Lonicerae japonicae Flos Using Response Surface Methodology (RSM)

Processes ◽  
2019 ◽  
Vol 7 (11) ◽  
pp. 854
Author(s):  
Jichang Li ◽  
Rui Wang ◽  
Zunlai Sheng ◽  
Zhiyong Wu ◽  
Chunli Chen ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Chlorogenic Acid ◽  
Response Surface ◽  
Extraction Process ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Single Factor ◽  
Solid Ratio ◽  
Scutellariae Radix ◽  
Optimized Conditions

In this study, a simultaneous water extraction process for baicalin, wogonoside, and chlorogenic acid has been optimized. The effect of extraction temperature, extraction time, and liquid–solid ratio was scrutinized by single factor experiments and further analyzed by Box–Behnken design (BBD) approach using response surface methodology (RSM). The extraction yield of investigated compounds was determined by high performance liquid chromatography (HPLC). Single-factor experiments and response surface analysis results revealed that the optimized conditions are: Liquid to solid ratio 25:1 (mL/g), extraction temperature 93 °C, extraction time 2.4 h, and the extraction cycle two. Importantly, it has been noted that under the above conditions, concentrations of baicalin, wogonoside, and chlorogenic were 0.078, 0.031, and 0.013 mg/mL, respectively, and the overall desirability (OD) value was 0.76 which was higher than the non-optimized conditions and the deviation from the predicted OD value was only 2.44%. Conclusively, it has been suggested that the model was stable and feasible, and fit for extraction of baicalin, wogonoside, and chlorogenic acid from Scutellariae Radix and Lonicerae (L.) japonicae Flos.

Optimization of Extraction Process for Carotenoids from Tamarillo Fruits by Response Surface Methodology

2013 ◽  
Vol 781-784 ◽  
pp. 1749-1754 ◽  
Author(s):  
Qing Song Yang ◽  
Yan Zhao
Keyword(s):  
Response Surface Methodology ◽  
Theoretical Prediction ◽  
Response Surface ◽  
Extraction Process ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Health Food ◽  
Liquid Ratio ◽  
Solid Liquid

Using Box-Behnken center composite design of three variables with three levels, the optimal extraction process for carotenoids from tamarillo fruits were determined by response surface methodology. The optimal extraction process was obtained as followed: solid-liquid ratio of 1:19.02(g/mL), extraction temperature of 44°C, and extraction time of 33.68 min. Under these conditions, the extraction of carotenoids content was 5.755[mg/100, similar to the theoretical prediction of 5.885[mg/100. This study confirms the efficiency of RSM as a simple and effective method and the great potential of tamarillo fruits as a health food and a resource of carotenoids.

Optimization of Extraction Process by Response Surface Methodology of Polysaccharides from Acanthopanax obouatus Hoo

2012 ◽  
Vol 512-515 ◽  
pp. 2386-2389
Author(s):  
Xiao Rong Li ◽  
Peng Zhao
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Extraction Process ◽  
Raw Material ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Predicted Values

Polysaccharide from Acanthopanax obouatus Hoo(AHPs) was extracted with the hot water.Box-Behnken design(BBD) was employed to optimize extraction temperature; extraction time and ratio of water to raw material to obtain a high AHPs yield. The yield of polysaccharide was 4.68% based on the above mentioned conditions.Close agreement between experimetal and predicted values was found.

Optimization of Extraction Condition of Mycelium Polysaccharide from Tricholoma mongolium by Response Surface Methodology

2011 ◽  
Vol 361-363 ◽  
pp. 770-776
Author(s):  
Xiao Tong Wu ◽  
Qi Wei Ren ◽  
Jie Zhang ◽  
Ji Zhao ◽  
Su Ting Han ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Hot Water ◽  
The Other ◽  
Extraction Temperature ◽  
Single Factor ◽  
Extraction Condition ◽  
Hot Water Extraction ◽  
Solid Ratio ◽  
Optimum Extraction

In this paper, the hot water extraction conditions of mycelium polysaccharide ofTricholoma mongoliumwere investigated. By single-factor experiments, ethanol amount was determined at three times and the other factors (liquid–solid ratio, extraction temperature and time) were chosen to further optimize extraction conditions using response surface methodology (RSM). The Center Composite experimental results showed the optimum extraction conditions as follows: a temperature of 85.38°C, a liquid–solid ratio of 31.78 g/ mL and a extraction time of 2.18 h. Under these conditions, two extractions sufficiently reached the maximal the rate of mycelium polysaccharide 7.23%.

scholarly journals Kinetic Modelling of Anthocyanin Extraction from Grape (Vitis vinifera) using Response Surface Methodology

2019 ◽  
Vol 8 (11) ◽  
pp. 3015-3019
Keyword(s):  
Response Surface Methodology ◽  
Vitis Vinifera ◽  
Response Surface ◽  
Kinetic Modelling ◽  
Extraction Process ◽  
Grape Pomace ◽  
High Yield ◽  
Total Phenolic ◽  
Natural Food ◽  
Extraction Temperature

Anthocyanins are natural food colorants and functional compound, those substitute synthetic colours. One of the major source of Anthocyanin is Grapes, and by implementing suitable extraction process, it is possible to obtain high yield extract. In our study, we have optimized anthocyanin extraction from grape pomace (Vitis vinifera) by using response surface methodology. Owing to its simple, economical and efficient alternative to other extraction process, this method of extraction was found to be appropriate. Various parameter combinations were performed to determine the anthocyanin concentration such as extraction temperature, extraction time and pH and the process was optimized and validated. Various characterization of grapes and anthocyanin such as moisture, ash, total phenolic content, quantification of anthocyanin, HPLC and FTIR were also performed

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