Research of Laser Induced Fluorescence Detection Techniques Based on Microfluidic Devices

2014 ◽  
Vol 989-994 ◽  
pp. 2761-2763
Author(s):  
Yong Qiang Cheng ◽  
Cui Lian Guo ◽  
Bin Zhao ◽  
Li Yang
Keyword(s):  
Detection Limit ◽  
Fluorescence Detection ◽  
Recent Progress ◽  
Biochemical Analysis ◽  
Detection Method ◽  
Microfluidic Devices ◽  
Laser Induced Fluorescence ◽  
Detection Techniques ◽  
Laser Induced Fluorescence Detection ◽  
Optical Structure

Laser induced fluorescence (LIF) detection technique is one of the most sensitive detection method in micro total analytical systems (μTAS) as its good monochromaticity, strong collimation and high optical density. It is widely used in biochemical analysis systems and the detection limit could reach 10-9-10-12mol/L. Due to the important role in μTAS, different optical structure arrangements of LIF detection techniques were continuously developed. We reviewed the recent progress of the optical structure arrangement and the applications of the LIF detection techniques.

scholarly journals Single-photon laser-induced fluorescence detection of nitric oxide at sub-parts-per-trillion mixing ratios

2020 ◽  
Vol 13 (5) ◽  
pp. 2425-2439 ◽  
Author(s):  
Andrew W. Rollins ◽  
Pamela S. Rickly ◽  
Ru-Shan Gao ◽  
Thomas B. Ryerson ◽  
Steven S. Brown ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Detection Limit ◽  
Fluorescence Detection ◽  
Narrow Band ◽  
Single Photon ◽  
Spectral Feature ◽  
Laser Induced Fluorescence ◽  
Mixing Ratios ◽  
Laser Induced Fluorescence Detection ◽  
Direct Measurements

Abstract. We describe a newly developed single-photon laser-induced fluorescence sensor for measurements of nitric oxide (NO) in the atmosphere. Rapid tuning of a narrow-band laser on and off of a rotationally resolved NO spectral feature near 215 nm and detection of the red-shifted fluorescence provides for interference-free direct measurements of NO with a detection limit of 1 part per trillion by volume (pptv) for 1 s of integration, or 0.3 pptv for 10 s of integration. Uncertainty in the sensitivity of the instrument is typically ±6–9 %, with no known interferences. Uncertainty in the zero of the detector is shown to be <0.2 pptv. The instrument was deployed on the NASA DC-8 aircraft during the NASA/NOAA FIREX-AQ experiment (Fire Influence on Regional to Global Environments Experiment – Air Quality) during July–September 2019 and provided more than 140 h of NO measurements over 22 flights, demonstrating the ability of this instrument to operate routinely and autonomously. Comparisons with a seasoned chemiluminescence sensor during FIREX-AQ in a variety of chemical environments provides validation and confidence in the accuracy of this technique.

scholarly journals Analysis of neurotransmitter catecholamines and related amines in human urine and serum by chromatography and capillary electrophoresis with 1, 3, 5, 7-tetramethyl-8-(N-hydroxysuccinimidyl propionic ester)-difluoro-boradiaza-s-indacene

2021 ◽  
Author(s):  
Liwei Cao ◽  
Lizhen Wu ◽  
Hailan Zhong ◽  
Hao Wu ◽  
Siyun Zhang ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Capillary Electrophoresis ◽  
Liquid Chromatography ◽  
Fluorescence Detection ◽  
High Performance ◽  
Detection Method ◽  
Laser Induced Fluorescence ◽  
Limits Of Detection ◽  
Laser Induced Fluorescence Detection ◽  
Derivatization Reaction

AbstractTwo sensitive and effective methods were developed for the detection of catecholamines and related biogenic amines (dopamine, epinephrine, norepinephrine, serotonin, levodopa and tyramine) using high performance liquid chromatography with fluorescence detection and capillary electrophoresis with laser-induced fluorescence detection. A BODIPY fluorescent dye, 1, 3, 5, 7-tetramethyl-8-(N-hydroxysuccinimidyl propionic ester)-difluoroboradiaza- s-indacene was used as pre-column derivatization reagent. The separation and derivatization conditions were optimized in detail. In high performance liquid chromatography with fluorescence detection method, the derivatization reaction was completed at 35 °C for 20 min. At the wavelength of λex/λem = 493 nm/513 nm, dopamine, epinephrine, norepinephrine, and levodopa derivatives achieved baseline separation within 15 min. The limits of detection (S/N = 3) were 1.0, 2.0, 5.0, and 0.5 nmol/L, respectively. In capillary electrophoresis with laser-induced fluorescence detection method, the derivatization reaction was completed at 25 °C for 20 min. Serotonin, tyramine and dopamine derivatives reached baseline separation within 10 min at the wavelength of λex = 473 nm. The limits of detection (S/N = 3) for serotonin, tyramine, and dopamine were 0.3, 0.02, and 0.2 nmol/L, respectively. The amino compounds in human serum and urine samples were detected successfully, and the recoveries were 93.3%–106.7% and 91.0%–103.1%, respectively.

scholarly journals Single-photon laser-induced fluorescence detection of nitric oxide at sub-parts per trillion mixing ratios

2020 ◽  
Author(s):  
Andrew W. Rollins ◽  
Pamela S. Rickly ◽  
Ru-Shan Gao ◽  
Thomas B. Ryerson ◽  
Steven S. Brown ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Detection Limit ◽  
Fluorescence Detection ◽  
Narrow Band ◽  
Single Photon ◽  
Spectral Feature ◽  
Laser Induced Fluorescence ◽  
Mixing Ratios ◽  
Laser Induced Fluorescence Detection ◽  
Direct Measurements

Abstract. We describe a newly developed single-photon laser-induced fluorescence sensor for measurements of nitric oxide (NO) in the atmosphere. Rapid tuning of a narrow-band laser on and off of a rotationally resolved NO spectral feature and detection of the red-shifted fluorescence provides for interference-free direct measurements of NO with a detection limit of 1 pptv for 1 second of integration, or 0.3 pptv for 10 seconds of integration. The instrument was deployed on the NASA DC-8 aircraft during the NASA FIREX-AQ experiment during July–September of 2019 and provided more than 140 hours of NO measurements over 22 flights, demonstrating the ability of this instrument to operate routinely and autonomously. Comparisons with a seasoned chemiluminescence sensor during FIREX-AQ in a variety of chemical environments provides validation and confidence of the accuracy of this technique.

Alcohol Determination by HPLC with Postcolumn Derivatization Based on the Thiosulfate-catalyzed Reaction of Alcohols and Cerium(IV) and Fluorescence Detection of Cerium(III)

2020 ◽  
Vol 16 ◽  
Author(s):  
Ikko Mikami ◽  
Eri Shibayama ◽  
Kengo Takagi
Keyword(s):  
Detection Limit ◽  
Fluorescence Detection ◽  
Reaction Rate ◽  
Detection Method ◽  
Fluorescence Detector ◽  
Reaction Solution ◽  
Optimum Detection ◽  
Postcolumn Derivatization ◽  
Sensitive Method

Background: Determination of a reducing substance based on the reaction between Ce(IV) and a reducing substance and fluorescence detection of Ce(III) generated has been reported as a selective and sensitive method. However, this method could not be applied to the determination of alcohol due to the low reaction rate of alcohol and Ce(IV). Objective: We found that thiosulfate catalytically enhanced reaction of alcohols (such as, methanol, ethanol, and propanol) and Ce(IV). Utilizing this effect, we developed a new method for the determination of alcohols. Results: In the presence of thiosulfate, an increase in fluorescence intensity was detected by injecting alcohol at concentrations of several millimolar, whereas it was not observed even at the concentration of 10% v/v (2 M for ethanol) in the absence of thiosulfate. The optimum detection conditions were determined to be 4.0 mM Ce(IV) sulfate and 0.50 mM thiosulfate, and the detection limit (S/N = 3) of ethanol under these conditions was 1 mM. In the calibration curves, changes in the slope were observed when the alcohol concentrations were approximately 10–25 mM. Using a thiosulfate solution containing ethanol as the reaction solution, a calibration curve without any change in slope was obtained, although the concentration of ethanol at the detection limit increased. The alcohols in the liquor and fuel were successfully analyzed using the proposed detection method as a postcolumn reaction. Conclusion: This new alcohol detection method using a versatile fluorescence detector can be applied to the postcolumn reaction of HPLC omitting need of time-consuming pretreatment processes.

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