scholarly journals High Performance Liquid Chromatography (HPLC) Analysis, Antioxidant, Antiaggregation of Mangosteen Peel Extract (Garcinia mangostana L.)

2014 ◽  
pp. 458-466
Author(s):  
Wahyu Widowati ◽  
◽  
Lusiana Darsono ◽  
Jo Suherman ◽  
Yelliantty Yelliantty ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Hplc Analysis ◽  
Garcinia Mangostana ◽  
Mangosteen Peel ◽  
Peel Extract

HPLC Analysis of Oxytetracycline Residues in Honey

1986 ◽  
Vol 49 (5) ◽  
pp. 383-388 ◽  
Author(s):  
PETER SPORNS ◽  
SUET KWAN ◽  
LAWRENCE A. ROTH
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Aqueous Solutions ◽  
High Performance ◽  
Hplc Analysis ◽  
Extraction Procedure ◽  
Hplc Method ◽  
Limits Of Detection ◽  
Ph Values ◽  
Double Extraction

Oxytetracycline (OTC), also known commercially as Terramycin, was determined to be more stable in honey than in buffered aqueous solutions at similar pH values and temperatures. A rapid high performance liquid chromatography (HPLC) method was developed to detect and quantitate OTC using a 1:1 dilution (wt/wt) of honey samples in water. Using 355 nm as the wavelength of detection, amounts as low as 0.5 μg/ml could be detected in the above solution. The limits of detection were lowered considerably by a double extraction procedure.

ANTHOCYANIN, AGLYCONE AND AGLYCONE-SUGAR CONTENT OF FRUITS OF TEMPERATE NORTH AMERICAN SPECIES OF Gaylussacia (ERICACEAE)

1988 ◽  
Vol 68 (1) ◽  
pp. 247-253 ◽  
Author(s):  
J. R. BALLINGTON ◽  
W. E. BALLINGER ◽  
E. P. MANESS
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
North American ◽  
High Performance ◽  
Hplc Analysis ◽  
Sugar Content ◽  
North American Species ◽  
The Other ◽  
Phylogenetic Implications

HPLC analysis of the true huckleberry species Gaylussacia baccata, G. dumosa, G. frondosa, G. mosieri, and G. ursina identified the 3-monoarabinosides, 3-monogalactosides, and 3-monoglucosides of cyanidin, delphinidin, malvidin, peonidin, and petunidin. Gaylussacia brachycera contained all anthocyanins, except peonidin-3-arabinoside. Gaylussacia brachycera differed from other species in percent delphinidin-3-arabinoside. It was higher than the other species in percent of the aglycone delphinidin and lower in cyanidin, and also higher in percent of the sugar arabinose. There were no detectable differences among the other species for anthocyanins, aglycones, or aglycone-sugars. The phylogenetic implications of the similarities among species of Gaylussacia and Vaccinium in anthocyanins, aglycones, and aglycone-sugars of the fruit were discussed.Key words: High-performance liquid chromatography, huckleberries, blueberries, chemotaxonomy, taxonomy, biosystematics

Effects of Baicalein on Prostanoid Generation from the Lung and Contractile Responses of the Trachea in Guinea Pig

1997 ◽  
Vol 25 (01) ◽  
pp. 37-50 ◽  
Author(s):  
Koji Miyamoto ◽  
Takeshi Katsuragi ◽  
Parhat Abdu ◽  
Tatsuo Furukawa
Keyword(s):  
High Performance Liquid Chromatography ◽  
Smooth Muscle ◽  
Liquid Chromatography ◽  
Guinea Pig ◽  
High Performance ◽  
Hplc Analysis ◽  
Antigen Challenge ◽  
Cyclooxygenase Inhibitor ◽  
Prostaglandin F ◽  
Stimulation Of

Effects of baicalein on release of slow reacting substance of anaphylaxis (SRS-A) or leukotriene (L T) from the sensitized guinea pig lung after antigen challenge and tonus of guinea pig tracheal muscles were studied. Baicalein inhibited release of SRS-A from sensitized guinea pig lung after antigen challenge. High-performance liquid chromatography (HPLC) analysis revealed that released SRS-A consisted of LTC 4 and D 4. Baicalein also reduced release of LTC 4 and D 4 from the sensitized lung after antigen challenge. Baicalein relaxed the isolated guinea pig tracheal smooth muscle contracted by LTD 4, carbachol or histamine. However, this compound produced a contraction when the tracheal muscle was contracted by prostaglandin F 2α ( PGF 2α). This contraction by baicalein was abolished by pretreatment with indomethacin, a cyclooxygenase inhibitor. Baicalein elicited a relaxation in the normal non-sensitized tracheal preparation but a contraction in the tissue isolated from actively sensitized guinea pig in 4 among 7 cases. Baicalein also produced a contraction in the trachea pretreated with phorbol dibutyrate and contracted by carbachol, which was eliminated after treatment with indomethacin. The results suggest that baicalein exerts action via, at least, two different mechanisms, the inhibition of releasing SRS-A (LTs) and direct relaxing effects on the trachea. Besides, baicalein seems to produce contraction under certain conditions, which may involve stimulation of the cyclooxygenase pathway.

Development of control material for hemoglobin analysis

2012 ◽  
Vol 50 (2) ◽  
Author(s):  
Sakorn Pornprasert ◽  
Kunyakan Kongthai ◽  
Jarurin Waneesorn ◽  
Kanokwan Jaiping ◽  
Kallayanee Treesuwan
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Hplc Analysis ◽  
Freeze Drying ◽  
Sucrose Solution ◽  
Control Material ◽  
Normal Individuals ◽  
Thalassemia Trait

AbstractThere is no certified control material for hemoglobin analysis which has the hemoglobin (Hb)AWashed and dialysed erythrocytes of normal individuals and patients with β-thalassemia trait, HbE trait, β-thalassemia/HbE disease, homozygous HbE were lysed in 5% sucrose solution. The lyophilized hemoglobin control materials were prepared by using a freeze-drying (lyophilization) method. The high performance liquid chromatography (HPLC) analysis of lyophilized hemoglobin was performed after storing at –20°C for 1, 15 and 30 days and for 3 months.The chromatograms of lyophilized hemoglobin control materials showed similar patterns and similar levels of HbA, HbAThe lyophilized hemoglobin could be developed and used as control materials for hemoglobin analysis.

scholarly journals Portrayal of Punica granatum L. peel extract through High Performance Liquid Chromatography and antimicrobial activity evaluation

2023 ◽  
Vol 83 ◽  
Author(s):  
M.S. Mahmood ◽  
A. Ashraf ◽  
S. Ali ◽  
A.B. Siddique ◽  
F. Asad ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Methanolic Extract ◽  
Punica Granatum ◽  
Diffusion Method ◽  
Staph Aureus ◽  
E Coli ◽  
Peel Extract

Abstract Increasing trend in antimicrobial resistance and failure of chemically synthesized antibiotics lead to discover alternative methods for the treatment of bacterial infections. Various medicinal plants are in use traditionally and their active compounds can be further applied for treatment of bacterial diseases. This study was designed to determine the antibacterial activity of Punica granatum (P. granatum L.) (pomegranate) peel extract against Enterobacteriaceae [Escherichia coli (E. coli), Salmonella Typhimurium (S. Typhimurium) and Shigella Dysenteriae (S. Dysenteriae)] and gram-positive bacterium [Staphylococcus aureus (Staph aureus)]. Methanolic extract of P. granatum L. peel was prepared by Soxhlet apparatus method. Total flavonoid and phenolic contents from the extract were determined by High Performance Liquid Chromatography (HPLC). The antibacterial activity of P. granatum L. peel extract was evaluated through agar well diffusion method. HPLC showed the range of phenolics (gallic acid, caffeic acid, benzoic acid, cinnamic acid) and flavonoid compounds. The chemical structures of flavonoid and phenolics found in the methanolic extract of P. granatum L. peel have been reported for the first time. The methanolic peel extract (50 ul) of yellow P. granatum L. showed 26, 10, 10 and 9mm zones of inhibition (ZOI) against S. aureus, S. Typhimurium, S. Dysenteriae and E. coli, respectively. The methanolic extract of red P. granatum L. (100 ul) showed 27, 8, 12 and 15 mm ZOI against Staph. aureus, S. Typhimurium, S. Dysenteriae and E. coli, respectively. Highest ZOI was observed against Staph. aureus. Many of the bacteria studied in the present work may cause serious gastrointestinal infections, which can lead to hemorrhagic diarrhea in children. These infections can be life-threatening to young children and the elderly. There is an incentive to find alternative control measures, such as plant and herbal extracts, especially in lesser-developed countries where traditional antibiotics may not be readily available.

Chlorination byproducts of 4,4′-methylene dianiline(MDA) and their mutagenicity

1994 ◽  
Vol 30 (10) ◽  
pp. 153-159 ◽  
Author(s):  
Y. Tsuchiya
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Thin Layer ◽  
High Performance ◽  
Hplc Analysis ◽  
Mutagenic Activity ◽  
Structural Formula ◽  
Reaction Products ◽  
Mutagenicity Test ◽  
Tlc Analysis

A mutagenicity study was carried out on 4,4′-methylene dianiline(MDA) an epoxy resin hardener and chlorinated MDA using an Ames Salmonella/microsome assay. As reaction products of MDA with chlorine in the water six spots were observed by thin layer chromatograph(TLC) and high performance liquid chromatography(HPLC) analysis revealed eighteen peaks on the HPLC chromatogram. MDA exhibited mutagenic activity for strain TA100 in the presence of S9 mix. In the mutagenicity test of chlorinated MDA corresponding to 7.8∼250 μg/plate, at 250 μg/plate the specimen showed mutagenic activity for strain TA98 without S9 mix, but more diluted specimens had no mutagenicity. At 31 and 250 μg/plate, the specimen showed weak mutagenic activity for the strain TA98 in the presence of S9 mix. The strain TA100 without S9 mix was killed on the culture plate with 250 μg/plate. However strong mutagenicity such as net revertant colonies 1270 per plate was obtained with the diluted specimen of 7.8 μg/plate. At 31 μg/plate, the specimen showed mutagenic activity for the strain TA100 with S9 mix. The mutagenicity test of five fractionated specimens from the TLC analysis, spot No. 1 and No. 2 showed no mutagenicity for strain TA98 and TA100 with and without S9 mix. The specimen of Spot No. 3 exhibited weak mutagenic activity for strain TA98 without S9 mix, the specimen of spot No. 4 at 125 μg/plate showed mutagenicity for strain TA98 and TA100 with and without S9 mix, and diluted samples showed high mutagenic activity for strain TA100 with and without S9 mix. Several newly identified mutagenic substances were found to be produced by the chlorination of MDA. GC/MS analysis of chlorinated MDA revealed eleven compounds on the chromatogram. Benzaldehyde, benzoquinones and chloroanilines were identified. However, the structural formula of the highly mutagenic substances could not be determined by GC/MS.

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