A Novel Method for Evaluating Compatibility of Hepatocytes with some Kinds of Biomaterials

2005 ◽  
Vol 288-289 ◽  
pp. 511-514
Author(s):  
Yingxin Xu ◽  
Wen Jie Wang ◽  
Yong Zhang ◽  
Xu Hua Song ◽  
Li Li ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Total Protein ◽  
Culture Medium ◽  
New Method ◽  
Morphological Observation ◽  
Hepatic Tissue ◽  
Feasible Method ◽  
Biochemical Analyzer ◽  
Novel Method ◽  
Automatic Biochemical Analyzer

To evaluate the biocompatibility of biomedical materials for hepatic tissue engineering, another new method was introduced to observe hepatocytes functions. In this experiment, hepatocytes were seeded onto four kinds of membranes of PLLA, PLGA (90:10), PLGA (75:25), and Chitosan cross-linked with collagen. The culture mediums were collected at 21 day after seeding, and then albumin (Alb), Urea (UN), glucose (Glu), total protein (TP), and triglyceride (TG) in the supernatant were detected by automatic biochemical analyzer. Results showed that hepatocytes on film of Chitosan cross-linked with collagen exhibited the highest level of TP and TG. These results were highly corresponding with the results of morphological observation. This data indicated that analyzing TP and TG in culture medium by automatic biochemical analyzer might be applied to evaluate hepatocytes biocompatibility on materials as a convenient and feasible method.

111 BIOCHEMICAL COMPOSITION OF FOLLICULAR FLUID IN RELATION TO THE STIMULUS TO INDUCE OVULATION IN ALPACAS (VICUGNA PACOS)

2017 ◽  
Vol 29 (1) ◽  
pp. 164 ◽  
Author(s):  
W. Huanca ◽  
A. Castro ◽  
N. Gomez ◽  
A. Cordero
Keyword(s):  
Follicular Fluid ◽  
Total Protein ◽  
Biochemical Composition ◽  
Ovarian Follicle ◽  
Follicular Development ◽  
Array Transducer ◽  
Vicugna Pacos ◽  
Continue Research ◽  
Biochemical Analyzer ◽  
Automatic Biochemical Analyzer

Alpacas, like other camelids, are induced ovulators. A study was designed to determine the effect of the ovulation-inducing stimulus on the biochemical composition of follicular fluid. Adult female alpacas (n = 18) were examined daily for 3 days by transrectal ultrasonography using a 5-MHz linear-array transducer (Aloka SSD-500, Tokyo, Japan). When the largest growing ovarian follicle was ≥7 mm, alpacas were given 1.0 mL of seminal plasma intramuscularly (SP, n = 9) or 40 µg of busereline acetate intramuscularly (GnRH, n = 9). A transvaginal transducer with an attached needle guide (Aloka UST-945BP-5) was used for collection of follicular fluid 22 h post-induction. Follicular contents were then centrifuged at 800 × g for 20 min to separate the fluid from the cells. The follicular fluid was collected and stored at –20°C until analysis with a semi-automatic biochemical analyzer (SINOWA, China). The results were glucose 49.17 and 47.95 (mg/dL; P > 0.05), total protein 1.85 and 1.15 (g/dL; P < 0.05), albumin 1.11 and 1.13 (g/dL; P > 0.05), triglycerides 3.94 and 3.16 (mg/dL; P > 0.05), cholesterol 39.01 and 42.5 (mg/dL; P > 0.05), phosphatase 32.68 and 21.36 (IU/L; P < 0.05), alanine aminotransferase 3.66 and 5.07 (IU/L; P > 0.05), and lactate dehydrogenase 42.17 and 27.27 (IU/L; P > 0.05) for SP or GnRH treatments, respectively. Results suggest the need to continue research to explain the effect of possible differences in total protein, cholesterol, and phosphatase on oocyte-expressed genes and follicular development. Research was supported by the project no. 405-PNICP-PIAP-2014-UNMSM.

scholarly journals Degradable hydrogels derived from PEG‐diacrylamide for hepatic tissue engineering

2015 ◽  
Vol 103 (10) ◽  
pp. 3331-3338 ◽  
Author(s):  
Kelly R. Stevens ◽  
Jordan S. Miller ◽  
Brandon L. Blakely ◽  
Christopher S. Chen ◽  
Sangeeta N. Bhatia
Keyword(s):  
Tissue Engineering ◽  
Hepatic Tissue

Hepatotoxic effects of chloroform extract of Artemisia macivera Linn in rats following intraperitoneal administration of different subchronic doses

2010 ◽  
Vol 30 (1) ◽  
pp. 25-33 ◽  
Author(s):  
SE Atawodi ◽  
AC Ene ◽  
DA Ameh
Keyword(s):  
Alkaline Phosphatase ◽  
Total Protein ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Intraperitoneal Administration ◽  
Chloroform Extract ◽  
Hepatic Tissue ◽  
Albino Rats ◽  
High Dose ◽  
Hepatocellular Injury

The possible hepatotoxic effects of chloroform extract of Artemisia maciverae was evaluated biochemically and histologically using male Swiss albino rats, randomly assigned into four groups of 24 animals each. The groups (control, 50, 100 and 200 mg/kg body weight) were treated for 60 days and then monitored for another 30 days before sacrifice. Alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, bilirubin (total and direct), total protein and albumin were assessed colorimetrically, while tissue specimens were subjected to histological examination following standard hematoxyline-eosin staining techniques. After 1 week of treatment, the extract caused statistically significant elevation in levels of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and bilirubin (total and direct), while there was significant (p < 0.05) decrease in the levels of serum total protein and albumin at the onset of treatment when compared with the control. These abnormalities in the levels of serum biochemical parameters were spontaneously corrected within 2 weeks of treatment. Similarly, histological assessment showed severe hepatic tissue injuries after 1 week, but these organs recovered spontaneously by the second week of treatment. The results indicate that long-term exposure to therapeutic doses of chloroform extract of A maciverae is relatively safe, but high dose exposure may result in hepatocellular injury.

scholarly journals Acanthamoeba-Campylobacter Coculture as a Novel Method for Enrichment of Campylobacter Species

2007 ◽  
Vol 73 (21) ◽  
pp. 6864-6869 ◽  
Author(s):  
Diana Axelsson-Olsson ◽  
Patrik Ellstr�m ◽  
Jonas Waldenstr�m ◽  
Paul D. Haemig ◽  
Lars Brudin ◽  
...  
Keyword(s):  
Culture Medium ◽  
Bacterial Species ◽  
Oxygen Quenching ◽  
Free Living ◽  
Promising Tool ◽  
Low Concentrations ◽  
Novel Method ◽  
Selective Antibiotics ◽  
Different Sources ◽  
Bacterial Concentrations

ABSTRACT In this study, we present a novel method to isolate and enrich low concentrations of Campylobacter pathogens. This method, Acanthamoeba-Campylobacter coculture (ACC), is based on the intracellular survival and multiplication of Campylobacter species in the free-living protozoan Acanthamoeba polyphaga. Four of the Campylobacter species relevant to humans and livestock, Campylobacter jejuni, C. coli, C. lari, and C. hyointestinalis, were effectively enriched by the coculture method, with growth rates comparable to those observed in other Campylobacter enrichment media. Studying six strains of C. jejuni isolated from different sources, we found that all of the strains could be enriched from an inoculum of fewer than 10 bacteria. The sensitivity of the ACC method was not negatively affected by the use of Campylobacter-selective antibiotics in the culture medium, but these were effective in suppressing the growth of seven different bacterial species added at a concentration of 104 CFU/ml of each species as deliberate contamination. The ACC method has advantages over other enrichment methods as it is not dependent on a microaerobic milieu and does not require the use of blood or other oxygen-quenching agents. Our study found the ACC method to be a promising tool for the enrichment of Campylobacter species, particularly from water samples with low bacterial concentrations.

Biocompatible Fibroin Blended Films with Recombinant Human-like Collagen for Hepatic Tissue Engineering

2006 ◽  
Vol 21 (1) ◽  
pp. 23-37 ◽  
Author(s):  
K. Hu ◽  
Q. Lv ◽  
F. Z. Cui ◽  
Q. L. Feng ◽  
X. D. Kong ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Hepatic Tissue

scholarly journals Strategic Team AI Path Plans: Probabilistic Pathfinding

2008 ◽  
Vol 2008 ◽  
pp. 1-6 ◽  
Author(s):  
Tng C. H. John ◽  
Edmond C. Prakash ◽  
Narendra S. Chaudhari
Keyword(s):  
Genetic Algorithm ◽  
Genetic Algorithms ◽  
Computer Games ◽  
Fitness Function ◽  
New Method ◽  
High Quality ◽  
Plan Generation ◽  
Novel Method ◽  
Games And Simulations

This paper proposes a novel method to generate strategic team AI pathfinding plans for computer games and simulations using probabilistic pathfinding. This method is inspired by genetic algorithms (Russell and Norvig, 2002), in that, a fitness function is used to test the quality of the path plans. The method generates high-quality path plans by eliminating the low-quality ones. The path plans are generated by probabilistic pathfinding, and the elimination is done by a fitness test of the path plans. This path plan generation method has the ability to generate variation or different high-quality paths, which is desired for games to increase replay values. This work is an extension of our earlier work on team AI: probabilistic pathfinding (John et al., 2006). We explore ways to combine probabilistic pathfinding and genetic algorithm to create a new method to generate strategic team AI pathfinding plans.

Liver Support Through Hepatic Tissue Engineering

1993 ◽  
pp. 92-107 ◽  
Author(s):  
Mehmet Toner ◽  
Ronald G. Tompkins ◽  
Martin L. Yarmush
Keyword(s):  
Tissue Engineering ◽  
Hepatic Tissue ◽  
Liver Support

Hepatic Tissue Engineering for Adjunct and Temporary Liver Support

2006 ◽  
pp. 59-1-59-19
Author(s):  
Fran√ßois Berthiaume ◽  
Arno W.Tilles ◽  
Martin L.Yarmush ◽  
Mehmet Toner ◽  
Christina Chan
Keyword(s):  
Tissue Engineering ◽  
Hepatic Tissue ◽  
Liver Support

scholarly journals Disjoint combinations profiling (DCP): a new method for the prediction of antibody CDR conformation from sequence

2014 ◽  
Author(s):  
Dimitris Nikoloudis ◽  
Jim E. Pitts ◽  
José W. Saldanha
Keyword(s):  
Protein Engineering ◽  
Prediction Accuracy ◽  
Variable Region ◽  
New Method ◽  
Engineering Applications ◽  
Variable Regions ◽  
Blind Prediction ◽  
Novel Method ◽  
Complementarity Determining Regions ◽  
Sequence Rules

The accurate prediction of the conformation of Complementarity-Determining Regions (CDRs) is important in modelling antibodies for protein engineering applications. Specifically, the Canonical paradigm has proved successful in predicting the CDR conformation in antibody variable regions. It relies on canonical templates which detail allowed residues at key positions in the variable region framework or in the CDR itself for 5 of the 6 CDRs. While no templates have as yet been defined for the hypervariable CDR-H3, instead, reliable sequence rules have been devised for predicting the base of the CDR-H3 loop. Here a new method termed Disjoint Combinations Profiling (DCP) is presented, which contributes a considerable advance in the prediction of CDR conformations. This novel method is explained and compared with canonical templates and sequence rules in a 3-way blind prediction. DCP achieved 93% accuracy over 951 blind predictions and showed an improvement in cumulative accuracy compared to predictions with canonical templates or sequence-rules. In addition to its overall improvement in prediction accuracy, it is suggested that DCP is open to better implementations in the future and that it can improve as more antibody structures are deposited in the databank. In contrast, it is argued that canonical templates and sequence rules may have reached their peak.

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