In Vitro Dissolution Behavior of Custom Made CaP Scaffolds for Bone Tissue Engineering

The degradation rate of custom made calcium phosphate scaffolds, designed for bone tissue engineering applications, influences the healing process of critical size bone defects. An optimal degradation rate exists at which the neo-formed bone replaces the CaP (calcium phosphate) scaffold [1]. Consequently investigating the complex degradation behavior (dissolution, reprecipitation, osteoclast activity) of custom made CaP structures gains interest. In this work different in vitro dissolution experiments were performed to study the degradation behavior of 4 by composition different calcium phosphates. Ideally these experiments should have a predictive power regarding the in vivo degradation behavior. In vitro dissolution tests still lack standardization. Therefore this study focuses on the influence of two dissolution constraints: (i) the material’s macrostructure (porous - dense), (ii) the regenerated fluid flow (bath shaking - perfusion). From 4 different CaP compositions porous structures and as a reference dense disks were produced, using the same starting powder and heat treatment. To compare the different dissolution tests, all data was normalized to the CaP surface area. Results show that besides the structural appearances of the CaP structures, also the design of the dissolution test influences the in vitro dissolution behavior. Moreover there is a need to take the morphology of the dissolved material into account. The CaP perfusion tests show dissolution dynamics that resemble the in vivo reality more closely than the shaking bath experiments.

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In Vitro Dissolution Behavior of Custom Made CaP Scaffolds for Bone Tissue Engineering

Bioceramics 20 - Key Engineering Materials ◽

10.4028/0-87849-457-x.7 ◽

2007 ◽

pp. 7-10

Author(s):

Saartje Impens ◽

Roosmarijn Schelstraete ◽

Steven Mullens ◽

Ivo Thijs ◽

Jan Luyten ◽

...

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

In Vitro Dissolution ◽

Dissolution Behavior ◽

Custom Made

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Long-Acting Risperidone Dual Control System: Preparation, Characterization and Evaluation In Vitro and In Vivo

Pharmaceutics ◽

10.3390/pharmaceutics13081210 ◽

2021 ◽

Vol 13 (8) ◽

pp. 1210

Author(s):

Xieguo Yan ◽

Shiqiang Wang ◽

Kaoxiang Sun

Keyword(s):

Drug Loading ◽

Entrapment Efficiency ◽

In Vitro Dissolution ◽

Dissolution Behavior ◽

In Vivo Evaluation ◽

Long Term Treatment ◽

Long Acting

Schizophrenia, a psychiatric disorder, requires long-term treatment; however, large fluctuations in blood drug concentration increase the risk of adverse reactions. We prepared a long-term risperidone (RIS) implantation system that can stabilize RIS release and established in-vitro and in-vivo evaluation systems. Cumulative release, drug loading, and entrapment efficiency were used as evaluation indicators to evaluate the effects of different pore formers, polymer ratios, porogen concentrations, and oil–water ratios on a RIS implant (RIS-IM). We also built a mathematical model to identify the optimized formulation by stepwise regression. We also assessed the crystalline changes, residual solvents, solubility and stability after sterilization, in-vivo polymer degradation, pharmacokinetics, and tissue inflammation in the case of the optimized formulation. The surface of the optimized RIS microspheres was small and hollow with 134.4 ± 3.5 µm particle size, 1.60 SPAN, 46.7% ± 2.3% implant drug loading, and 93.4% entrapment efficiency. The in-vitro dissolution behavior of RIS-IM had zero-order kinetics and stable blood concentration; no lag time was released for over three months. Furthermore, the RIS-IM was not only non-irritating to tissues but also had good biocompatibility and product stability. Long-acting RIS-IMs with microspheres and film coatings can provide a new avenue for treating schizophrenia.

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In Vitro Dissolution Behavior of Biphasic Tricalcium Phosphate Composite Powders Composed of α-Tricalcium Phosphate and β-Tricalcium Phosphate

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.368-372.1206 ◽

2008 ◽

Vol 368-372 ◽

pp. 1206-1208 ◽

Cited By ~ 11

Author(s):

Yan Bao Li ◽

Dong Xu Li ◽

Wen Jian Weng

Keyword(s):

Heat Treatment ◽

Calcium Phosphate ◽

Tricalcium Phosphate ◽

Amorphous Calcium Phosphate ◽

Ph Value ◽

In Vitro Dissolution ◽

Dissolution Behavior ◽

Buffer Solution ◽

Composite Powders

Biphasic tricalcium phosphate (BTCP) powders composed of α-tricalcium phosphate (α-TCP) and β-tricalcium phosphate (β-TCP) were prepared using amorphous calcium phosphate (ACP) precursor after heat treatment at 800oC. The in vitro dissolution behavior of the powders was examined after soaked in 0.1M NaAc-HAc buffer solution for different times. It was revealed that the Ca2+ and PO4 3- concentration, and pH value of the BTCP-soaked solution are higher than those of the α-TCP- and β-TCP-soaked solutions. The dissolution behavior of BTCP powders was explained. The specific dissolution behavior of BTCP powders can widen the biodegradation range of calcium phosphate family.

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Evaluation of different in vitro dissolution tests based on level A in vitro–in vivo correlations for fenofibrate self-emulsifying lipid-based formulations

European Journal of Pharmaceutics and Biopharmaceutics ◽

10.1016/j.ejpb.2016.10.030 ◽

2017 ◽

Vol 112 ◽

pp. 18-29 ◽

Cited By ~ 17

Author(s):

Aude Pestieau ◽

Sonia Lebrun ◽

Bernard Cahay ◽

Adeline Brouwers ◽

Bruno Streel ◽

...

Keyword(s):

In Vitro Dissolution ◽

Dissolution Tests

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Celecoxib confinement within mesoporous silicon for enhanced oral bioavailability

Open Material Sciences ◽

10.2478/mesbi-2013-0001 ◽

2014 ◽

Vol 1 (1) ◽

Cited By ~ 4

Author(s):

Feng Wang ◽

Timothy J. Barnes ◽

Clive A. Prestidge

Keyword(s):

Physicochemical Characteristics ◽

In Vitro Dissolution ◽

Absolute Bioavailability ◽

Dissolution Behavior ◽

Pharmacokinetic Parameters ◽

Mesoporous Silicon ◽

Silicon Matrix ◽

Oxidized Porous Silicon

AbstractWe investigate the physicochemical characteristics of celecoxib (CEL) entrapped within particles of an oxidized porous silicon matrix (pSiox); determine the oral dose response of CEL compared to pure drug and innovator formulation; develop in vivo-in vitro correlation (IVIVC). CEL was loaded into a pSiox matrix by solvent partitioning, with the physical state of the CEL characterized by FTIR, DSC, TGA and XRD, and correlated with in vitro dissolution behavior. Single dose pharmacokinetic parameters of orally dosed CEL were determined in fasted rats for aqueous suspensions of pure CEL, Celebrexr and CEL-pSiox microparticles. Physicochemical testing of CEL-pSiox formulation confirmed the entrapment of CEL within porous nanostructure in an amorphous or non-crystalline form. CEL-pSiox demonstrated superior pharmacokinetics compared with CEL particles or Celebrexr, i.e. increased absolute bioavailability (96.2% vs. 65.2% vs. 88.1%), increased C

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Preliminary Investigation of the Dissolution Behavior, Cytocompatibility, Effects of Fibrinogen Conformation and Platelet Adhesion for Radiopaque Embolic Particles

10.32920/14637957 ◽

2021 ◽

Author(s):

Sharon Kehoe ◽

Marie-Laurence Tremblay ◽

Aisling Coughlan ◽

Mark R. Towler ◽

Jan K. Rainey ◽

...

Keyword(s):

Platelet Adhesion ◽

Glass Structure ◽

Preliminary Investigation ◽

Arterial Embolization ◽

In Vitro Dissolution ◽

Dissolution Behavior ◽

Ion Release ◽

Initial Release

Experimental embolic particles based on a novel zinc-silicate glass system have been biologically evaluated for potential consideration in transcatheter arterial embolization procedures. In addition to controlling the cytotoxicity and haemocompatibility for such embolic particles, its glass structure may mediate specific responses via dissolution in the physiological environment. In a 120 h in-vitro dissolution study, ion release levels for silicon (Si4+), sodium (Na+), calcium (Ca2+), zinc (Zn2+), titanium (Ti4+), lanthanum (La3+), strontium (Sr2+), and magnesium (Mg2+), were found to range from 0.04 to 5.41 ppm, 0.27–2.28 ppm, 2.32–8.47 ppm, 0.16–0.20 ppm, 0.12–2.15 ppm, 0.16–0.49 ppm and 0.01–0.12 ppm, respectively for the series of glass compositions evaluated. Initial release of Zn2+ (1.93–10.40 ppm) was only evident after 120 h. All compositions showed levels of cell viabilities ranging from 61.31 ± 4.33% to 153.7 ± 1.25% at 25%–100% serial extract dilutions. The conformational state of fibrinogen, known to induce thrombi, indicated that no changes were induced with respect of the materials dissolution by-products. Furthermore, the best-in-class experimental composition showed equivalency to contour PVA in terms of inducing platelet adhesion. The data generated here provides requisite evidence to continue to in-vivo pre-clinical evaluation using the best-in-class experimental composition evaluated.

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Regenerating Craniofacial Dental Defects With Calcium Phosphate Cement Scaffolds: Current Status and Innovative Scope Review

Frontiers in Dental Medicine ◽

10.3389/fdmed.2021.743065 ◽

2021 ◽

Vol 2 ◽

Author(s):

Rashed A. Alsahafi ◽

Heba Ahmed Mitwalli ◽

Abdulrahman A. Balhaddad ◽

Michael D. Weir ◽

Hockin H. K. Xu ◽

...

Keyword(s):

Tissue Engineering ◽

Calcium Phosphate ◽

Bone Regeneration ◽

Calcium Phosphate Cement ◽

Current Status ◽

Craniofacial Bone ◽

Calcium Phosphate Cements ◽

And Performance

The management and treatment of dental and craniofacial injuries have continued to evolve throughout the last several decades. Limitations with autograft, allograft, and synthetics created the need for more advanced approaches in tissue engineering. Calcium phosphate cements (CPC) are frequently used to repair bone defects. Since their discovery in the 1980s, extensive research has been conducted to improve their properties, and emerging evidence supports their increased application in bone tissue engineering. This review focuses on the up-to-date performance of calcium phosphate cement (CPC) scaffolds and upcoming promising dental and craniofacial bone regeneration strategies. First, we summarized the barriers encountered in CPC scaffold development. Second, we compiled the most up to date in vitro and in vivo literature. Then, we conducted a systematic search of scientific articles in MEDLINE and EMBASE to screen the related studies. Lastly, we revealed the current developments to effectively design CPC scaffolds and track the enhanced viability and therapeutic efficacy to overcome the current limitations and upcoming perspectives. Finally, we presented a timely and opportune review article focusing on the significant potential of CPC scaffolds for dental and craniofacial bone regeneration, which will be discussed thoroughly. CPC offers multiple capabilities that may be considered toward the oral defects, expecting a future outlook in nanotechnology design and performance.

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Preliminary Investigation of the Dissolution Behavior, Cytocompatibility, Effects of Fibrinogen Conformation and Platelet Adhesion for Radiopaque Embolic Particles

10.32920/14637957.v1 ◽

2021 ◽

Author(s):

Sharon Kehoe ◽

Marie-Laurence Tremblay ◽

Aisling Coughlan ◽

Mark R. Towler ◽

Jan K. Rainey ◽

...

Keyword(s):

Platelet Adhesion ◽

Glass Structure ◽

Preliminary Investigation ◽

Arterial Embolization ◽

In Vitro Dissolution ◽

Dissolution Behavior ◽

Ion Release ◽

Initial Release

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A STELLA simulation model for in vitro dissolution testing of respirable size particles

Scientific Reports ◽

10.1038/s41598-019-55164-0 ◽

2019 ◽

Vol 9 (1) ◽

Author(s):

Basanth Babu Eedara ◽

Ian G. Tucker ◽

Shyamal C. Das

Keyword(s):

Simulation Model ◽

In Vitro Dissolution ◽

Membrane Thickness ◽

Dissolution Testing ◽

Custom Made ◽

Quality Control Tool ◽

Modeling Software ◽

Fast Dissolution

AbstractIn vitro dissolution testing is a useful quality control tool to discriminate the formulations and to approximate the in vivo drug release profiles. A dissolution apparatus has been custom-made for dissolution testing of dry powder formulations in a small volume of stationary medium (25 μL spread over 4.91 cm2 area i.e. ~50 μm thick). To understand the system and predict the key parameters which influence the dissolution of respirable size particles, a simulation model was constructed using STELLA modeling software. Using this model, the permeation (dissolution followed by diffusion through the membrane) of two anti-tubercular drugs of differing solubilities, moxifloxacin (17.68 ± 0.85 mg mL−1) and ethionamide (0.46 ± 0.02 mg mL−1), from the respirable size particles and their diffusion from a solution were simulated. The simulated permeation profiles of moxifloxacin from solution and respirable size particles were similar, indicating fast dissolution of the particles. However, the simulated permeation profile of ethionamide from respirable size particles showed slower permeation compared to the solution indicating the slow dissolution of the respirable size particles of ethionamide. The sensitivity analysis suggested that increased mucus volume and membrane thickness decreased the permeation of drug. While this model was useful in predicting and distinguishing the dissolution behaviours of respirable size moxifloxacin and ethionamide, further improvement could be made using appropriate initial parameter values obtained by experiments.

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