Effect of the Pore Structure of an Apatite-Fiber Scaffold on the Differentiation of P19.CL6 Cells into Cardiomyocytes

2018 ◽  
Vol 782 ◽  
pp. 116-123
Author(s):  
Yuzuha Ichikawa ◽  
Kei Yasuda ◽  
Masahiro Takahara ◽  
Mamoru Aizawa ◽  
Nobuyuki Kanzawa

We previously reported that P19.CL6 cells can be cultured in porous hydroxyapatite ceramics prepared by firing green compacts consisting of apatite fibers and spherical carbon beads (150 μm in diameter). Cells cultured for 20 days in an apatite-fiber scaffold (AFS) proliferated and differentiated into cells expressing troponin T, a cardiomyocyte-specific gene, but the expression level was insufficient to support the functional maturation of cells required for biomedical device applications. In this study, we aimed to optimize the internal AFS environment for cardiomyocytes by mixing two sizes (150-and 20-μm) of carbon beads. P19.CL6 cells were cultured in AFS materials comprising different carbon ratios in the presence of alpha-MEM with (AFS+) or without (AFS-) dimethyl sulfoxide (DMSO), and cell growth and gene expression were assessed. We found that AFS(50, 1:1 ratio) is the most suitable scaffold for the proliferation and differentiation of P19.CL6 cells and the addition of DMSO to the culture medium is necessary for differentiation into cardiomyocytes. We also assessed the culture of P19.CL6 cells in AFS in a radial-flow bioreactor for several days.

2018 ◽  
Vol 768 ◽  
pp. 135-139 ◽  
Author(s):  
Xin Yi Zhao ◽  
Xiao Peng Li ◽  
Kang Zhao ◽  
Yu Fei Tang

The objectives of this study are to prepare a porous hydroxyapatite ceramic which has dentin tubule-like structure and determine its permeability. Slurry of hydroxyapatite powder, deionized water and a binder was poured into a ring which was placed on a freezing plate.The hydroxyapatite slurry was freezed in a certain rate (by controlling the temperature of the freeze plate at −15°C, −30°C and −45°C) for a certain period of time, then the freezed sample was freezing dried to remove the frozen vehicle, followed by being sintered at 1250 °C for 2 h. After that,the morphology of the cross section and longitudinal section of the sintered porous hydroxyapatite ceramic was observed by SEM and the hydraulic conductance of cross section discs of the sintered porous hydroxyapatite were determined using a self-made micro-flowing permeability tester. Results showed that the prepared hydroxyapatite ceramics having bottom-up unidirectional comblike tubule structure and the tubule diameters associated with the temperature of freezing plate.The ceramic discs prepared on the freezing plate of −45°C exhibited similarity to nature dentin tubule, with a diameter of 9.72±3.41mm and a hydraulic conductance of 0.16±0.09 ml×min-1×cm-2×cm×H2O-1.


Bioceramics ◽  
1997 ◽  
pp. 595-598
Author(s):  
K. Mishima ◽  
H. Ohgushi ◽  
T. Yoshikawa ◽  
H. Nakajima ◽  
E. Yamada ◽  
...  

Author(s):  
Chikako Ikeda ◽  
Mikihiro Ueki ◽  
Satoshi Nakamura ◽  
Takayuki Kobayashi ◽  
Kimihiro Yamashita

2013 ◽  
Vol 32 (5) ◽  
pp. 753-760 ◽  
Author(s):  
Masahiko MINAMI ◽  
Masaaki TAKECHI ◽  
Kouji OHTA ◽  
Akira OHTA ◽  
Yoshiaki NINOMIYA ◽  
...  

2013 ◽  
Vol 587 ◽  
pp. 39-42 ◽  
Author(s):  
Yoshiyuki Yokogawa ◽  
S. Ito ◽  
Y. Yamato ◽  
I. Kishida

The preparation of mesoporous silica coated hydroxyapatite (HAp) ceramics is reported. The adsorption of proteins onto hydroxyapatite (HAp) is important in a variety of oral or osseous biological events. HAp has been used as bone filler, and the protein adsorption onto HAp materials is of importance for the bond formation. HAp has been also used as the material for column packing of liquid chromatography to separate and refine proteins due to the adsorptive capabilities with respect to the HAp. Mesoporous silica (MPS) materials have been extensively studied as carriers of bio-molecules due to their potential practical applications in medical materials. Pore diameters of 1.5 30 nm of the periodic mesoporous materials are close to the diameters of target molecules and enclosure of the protein in a well-defined space may help to prevent denaturation. Previously, we reported the MPS coating on the porous hydroxyapatite (HAp) ceramics obtained by dip-coating method. Protein adsorption on the porous HAp with the MPS coatings was evaluated using UV-VIS spectrometry, and the quantity of BSA adsorbed onto the HAp ceramics with the MPS coatings is around 1.5 times high compared to that on porous HAp ceramics. In this study, the vapor-phase synthesis of MPS film on HAP ceramics was carried out to improve the protein adsorptive property of HAp ceramics. The quantity of adsorbed protein onto the HAp ceramic disc with MPS coatings is around 5 times high compared to that on HAp ceramic disc.


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