scholarly journals Rapid mass propagation of endangered valuable medicinal plant Salacia chinensis L. and GC-MS/LC-MS analysis of active compounds produced in callus and leaf extracts

2021 ◽  
Vol 6 (2) ◽  
pp. 1
Author(s):  
S. G. Kamat ◽  
R. Vasudeva ◽  
C. G. Patil
Keyword(s):  
Medicinal Plant ◽  
Mass Propagation ◽  
Leaf Extracts ◽  
Active Compounds ◽  
Ms Analysis ◽  
Rapid Mass

Gc-ms analysis of active compounds of cinnamon leaf extracts (cinnamomum burmanni blume)

2017 ◽  
Vol 8 (2) ◽  
Author(s):  
ANAK AGUNG KETUT DARMADI ◽  
DEWA NGURAH SUPRAPTA ◽  
I GEDE RAI MAYA TEMAJA ◽  
I MADE DIRA SWANTARA
Keyword(s):  
Leaf Extracts ◽  
Active Compounds ◽  
Ms Analysis

In-vitro bactericidal activity of a novel plant source Plumeria pudica against some human and fish pathogenic bacteria

2020 ◽  
Vol 17 ◽  
Author(s):  
Shubhaisi Das ◽  
Sunanda Burman ◽  
Goutam Chandra
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Positive Control ◽  
Leaf Extracts ◽  
Ms Analysis ◽  
Ft Ir ◽  
Aldehyde Groups

Background: The only remedy for up surging problem of antibiotic resistance is the discovery of antibacterial agents of natural origin. Objective: The present study was aimed at finding antibacterial potential of crude and solvent extracts of mature leaves of Plumeria pudica. Methods: Antibacterial activity of three different solvent extracts were evaluated in four human and four fish pathogenic bacteria by measuring the zone of inhibition and determining Minimum Inhibitory Concentration and Minimum Bactericidal Concentration values. Standard antibiotics were used as positive control. Preliminary phytochemical screening of most effective extract i.e., ethyl acetate extract, Fourier Transform Infra Red analysis and GC-MS analysis of the Thin Layer Chromatographic (TLC) fraction of ethyl acetate extract were done meticulously. All experiments were done thrice and analyzed statistically. Results: Crude leaf extracts and solvent extracts caused good inhibition of bacterial growth in all selected bacteria. Ethyl acetate extract showed highest inhibition zones in all tested strains with maximum inhibition (19.50±0.29 mm) in Escherichia coli (MTCC 739). MBC/MIC of the extracts indicated that all three solvent extracts were bactericidal. Preliminary phytochemical tests revealed the presence of tannins, steroids and alkaloids and FT-IR analysis revealed presence of many functional groups namely alcoholic, amide, amine salt and aldehyde groups. From the GC-MS analysis of TLC fraction of ethyl acetate extract five different bioactive compounds e.g., 2,4-ditert –butylphenyl 5-hydroxypentanoate, Oxalic acid; allyl nonyl ester, 7,9-Ditert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione, Dibutyl phthalate and 2,3,5,8-tetramethyl-decane were identified. Conclusion: Leaf extracts of P. pudica contain bioactive compounds that can be used as broad spectrum bactericidal agent.

LC-DAD-ESI-MS/MS analysis, cytotoxic and antiproliferative effect of chlorogenic acid derivates rich extract from Nerium oleander L. pink flowers

2021 ◽  
Author(s):  
Siham Ayouaz ◽  
Sheila Cristina Oliveira-Alves ◽  
Ana Teresa Serra ◽  
Khalef LEFSIH ◽  
Madani Samah ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Chlorogenic Acid ◽  
Antiproliferative Effect ◽  
Nerium Oleander ◽  
Phenolic Composition ◽  
Esi Ms ◽  
Ms Analysis

Nerium oleander L, is a medicinal plant widely used for pharmaceutical purposes. In this work the pink flowers of this plant were characterized in terms of phenolic composition by LC-DAD-ESI-MS/MS...

scholarly journals GC-MS ANALYSIS OF PHYTOCHEMICAL CONSTITUENTS IN THE PETROLEUM ETHER LEAF EXTRACTS OF MILLETTIA PEGUENSIS

2017 ◽  
Vol 8 (9) ◽  
pp. 144-150 ◽  
Author(s):  
Manikandan G ◽  
Vimala Rani A ◽  
Divya C ◽  
Ramasubbu R
Keyword(s):  
Petroleum Ether ◽  
Leaf Extracts ◽  
Ms Analysis ◽  
Phytochemical Constituents

scholarly journals LARVICIDAL ACTIVITY OF LANTANA INDICA AND VITEX NEGUNDO ON CULEX QUINQUEFASCIATUS

2018 ◽  
Vol 11 (5) ◽  
pp. 414
Author(s):  
Rathnasagar K ◽  
Thiyagaraj Anand
Keyword(s):  
Larvicidal Activity ◽  
Culex Quinquefasciatus ◽  
Binding Protein ◽  
Inhibitory Effect ◽  
Negative Control ◽  
Vitex Negundo ◽  
Leaf Extracts ◽  
Active Compounds ◽  
Sterol Binding

Objectives: The activity of two different leaf extracts of Lantana indica and Vitex negundo is tested against the 3rd and 4th instar Culex quinquefasciatus larvae to evaluate the potency of the extracts as a larvicide and to find an ecologically sustainable alternative to chemical insecticides. A bioinformatics screening approach was performed to evaluate the in vivo results.Methods: The obtained larvae’s from nearby water sources were tested with N, N-diethyl-meta-toluamide (DEET) as the positive control which is the commercial chemical mosquito repellent and the solvents used for the respective plant extracts act as the negative control. Petroleum ether (PE), ethyl acetate (EA) and an aqueous (AQ) extract were prepared for both L. indica and V. negundo extracts, and its larvicidal activity was tested. A docking based approach was used to study the inhibitory effect of known active compounds from L. indica and V. negundo against acetylcholine esterase (AChE) and sterol binding protein as targets.Results: On comparing the results between three plants extract for its larvicidal activity, the EA extract of V. negundo and L. indica is found to be potent with a low LC50 value. Further, the docking studies between active compounds of L. indica and V. negundo with AChE and Sterol binding protein as targets showed that the compound tangeritin-1 had a good docking score compared to DEET and could be a natural alternative for larvicidal activity in the mosquito.Conclusion: Individual activity of tangeritin-1 could be further studied with mosquito mortality studies and molecular simulations and develop tangeritin-1 as a potential larvicidal compound for commercial use.

RAPID MASS PROPAGATION THROUGH MULTIPLE SHOOT INDUCTION FROM RHIZOME OF CHINESE CYMBIDIUMS

2010 ◽  
pp. 213-217 ◽  
Author(s):  
H.-F. Chiang ◽  
J.-R. Lin ◽  
C.-Y. Kao ◽  
K.-S. Liu ◽  
Y. Chu
Keyword(s):  
Multiple Shoot ◽  
Shoot Induction ◽  
Mass Propagation ◽  
Multiple Shoot Induction ◽  
Rapid Mass

scholarly journals GC-MS Analysis and Antimicrobial Activities of Ethanol Alkaloid Leaf Extracts of Delonix elata.L

2021 ◽  
Vol 68 (2) ◽  
Author(s):  
D. Muthuselvam ◽  
Kathick Kathick
Keyword(s):  
Present Finding ◽  
Cell Formation ◽  
Multidrug Resistant ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Leaf Extracts ◽  
Ms Analysis ◽  
Escherchia Coli ◽  
Physiological Abnormalities

Delonix elata L., belongs to family Fabaceae used by the traditional various medicinal practices to cure jaundice, skin disease, heart disease, cancer cell formation, physiological abnormalities, heptoprotective, bronchial and rheumatic problems. The present study was screen the antimicrobial and phytochemical activity of alkaloid leaf extracts. This extracts was assessed on multidrug resistant clinical isolated from both gram positive, gram negative and antifungal strains including Bacillus subtilis, Staphylococcus aureus, Escherchia coli, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger. The zone of inhibition was determined by Agar well diffusion method with various concentration. GC- MS analysis was performed to identify major bioactive compounds present in the extracts. The GC – MS studies shown the present of 25 compound were identified in the leaf extract composition. The antimicrobial analysis revealed that C. albicans showed a highest zone of inhibition 25mm at 100 mg/ml of extracts. Present finding suggest that D. elata as plant pharmaceutical and pharmacological importance.

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