scholarly journals Cellular Interactions of Synovial Fluid γδ T Cells in Juvenile Idiopathic Arthritis

2012 ◽  
Vol 188 (9) ◽  
pp. 4349-4359 ◽  
Author(s):  
Anna Bendersky ◽  
Victoria Marcu-Malina ◽  
Yackov Berkun ◽  
Maya Gerstein ◽  
Meital Nagar ◽  
...  
Keyword(s):  
T Cells ◽  
Juvenile Idiopathic Arthritis ◽  
Synovial Fluid ◽  
Γδ T Cells ◽  
Cellular Interactions

scholarly journals Evidence of the transient nature of the Th17 phenotype of CD4+CD161+ T cells in the synovial fluid of patients with juvenile idiopathic arthritis

2011 ◽  
Vol 63 (8) ◽  
pp. 2504-2515 ◽  
Author(s):  
Lorenzo Cosmi ◽  
Rolando Cimaz ◽  
Laura Maggi ◽  
Veronica Santarlasci ◽  
Manuela Capone ◽  
...  
Keyword(s):  
T Cells ◽  
Juvenile Idiopathic Arthritis ◽  
Synovial Fluid ◽  
Transient Nature

scholarly journals High Expression of Fas Ligand by Synovial Fluid-Derived γδ T Cells in Lyme Arthritis

2003 ◽  
Vol 170 (5) ◽  
pp. 2702-2710 ◽  
Author(s):  
Karen Roessner ◽  
Julie Wolfe ◽  
Cuixia Shi ◽  
Leonard H. Sigal ◽  
Sally Huber ◽  
...  
Keyword(s):  
T Cells ◽  
Synovial Fluid ◽  
Fas Ligand ◽  
Γδ T Cells ◽  
Lyme Arthritis ◽  
High Expression

Sa.24. Induction, Expansion and Characterisation of T Cells with a Regulatory Phenotype from Synovial Fluid of Patients with Juvenile Idiopathic Arthritis

2008 ◽  
Vol 127 ◽  
pp. S88
Author(s):  
Markus Haug ◽  
Nicole Bieder ◽  
Nikolay Tzaribachev ◽  
Toni Hospach ◽  
Joachim Peitz ◽  
...  
Keyword(s):  
T Cells ◽  
Juvenile Idiopathic Arthritis ◽  
Synovial Fluid ◽  
Regulatory Phenotype

scholarly journals Innate Lymphoid Cells and T Cells Contribute to the Interleukin‐17A Signature Detected in the Synovial Fluid of Patients With Juvenile Idiopathic Arthritis

2019 ◽  
Vol 71 (3) ◽  
pp. 460-467 ◽  
Author(s):  
Elizabeth C. Rosser ◽  
Hannah Lom ◽  
David Bending ◽  
Chantal L. Duurland ◽  
Mona Bajaj‐Elliott ◽  
...  
Keyword(s):  
T Cells ◽  
Juvenile Idiopathic Arthritis ◽  
Synovial Fluid ◽  
Innate Lymphoid Cells ◽  
Lymphoid Cells ◽  
Interleukin 17A

scholarly journals Th17 Transcription Factor RORC2 Is Inversely Correlated with FOXP3 Expression in the Joints of Children with Juvenile Idiopathic Arthritis

2009 ◽  
Vol 36 (9) ◽  
pp. 2017-2024 ◽  
Author(s):  
BIAGIO OLIVITO ◽  
GABRIELE SIMONINI ◽  
SARA CIULLINI ◽  
MARIA MORIONDO ◽  
LETIZIA BETTI ◽  
...  
Keyword(s):  
T Cells ◽  
Transcription Factor ◽  
Juvenile Idiopathic Arthritis ◽  
Synovial Fluid ◽  
Inverse Relationship ◽  
Treg Cells ◽  
Interleukin 17 ◽  
Mrna Levels ◽  
Foxp3 Mrna ◽  
Transcript Levels

Objective.To investigate the relationship between interleukin 17 (IL-17) producing T cells (Th17) and CD4+CD25+FOXP3+ regulatory T cells (Tregs) in blood and synovial fluid (SF) of patients with juvenile idiopathic arthritis (JIA).Methods.Sixty-five children with JIA (18 males and 47 females, median age 6.2 yrs; 45 with oligoarticular and 20 with polyarticular course) and 75 age- and sex-matched healthy controls were studied. Flow cytometry was used to analyze the forkhead box P3 (FOXP3)-positive Treg cells in peripheral blood (PB) and synovial fluid mononuclear cells (SFMC). FOXP3 and retinoic-acid related orphan receptor C isoform 2 (RORC2) messenger RNA (mRNA) were assessed by real-time polymerase chain reaction analysis. Cytokines (IL-17 and Th1/Th2 related cytokines) were measured in culture supernatants of 11 paired PBMC and SFMC activated with PMA and ionomycin.Results.FOXP3+ T cells and FOXP3 mRNA amounts were significantly lower in PB of children with JIA as compared with controls (p = 0.0002 and p = 0.001, respectively) and a higher percentage of Treg cells with concomitant higher level of FOXP3 transcript levels were observed in SF when compared with their PB counterparts (both p < 0.0001). SF CD4+FOXP3+ T cells were characterized by higher amounts of FOXP3 protein per cell when compared with peripheral CD4+FOXP3+ T cells, as revealed by the difference in FOXP3 median fluorescence intensity (median ± SD, arbitrary units, 54 ± 22.6 vs 19.5 ± 4.2; p < 0.001). RORC2 transcript levels were higher in JIA joints when compared with matched PB samples (median fold increase 3.9, p < 0.0001) but negatively correlated with FOXP3 mRNA levels (r = −0.623, p = 0.04). Stimulated SFMC displayed an impaired ability to produce IL-17 when compared with PBMC and, interestingly, an inverse relationship between IL-17 levels and the percentage of CD4+CD25+FOXP3+ SF T cells (r = −0.510, p = 0.047) was seen.Conclusion.We demonstrated for the first time an increased synovial expression of the transcription factor of Th17, RORC2, in JIA, and its inverse relationship with FOXP3 mRNA. These results extend research on “Th17” and Tregs in JIA.

scholarly journals Increased autophagy contributes to the inflammatory phenotype of juvenile idiopathic arthritis synovial fluid T cells

Rheumatology ◽  
2017 ◽  
Vol 56 (10) ◽  
pp. 1694-1699 ◽  
Author(s):  
Janneke G. C. Peeters ◽  
Nienke de Graeff ◽  
Martin Lotz ◽  
Salvatore Albani ◽  
Sytze de Roock ◽  
...  
Keyword(s):  
T Cells ◽  
Juvenile Idiopathic Arthritis ◽  
Synovial Fluid ◽  
Inflammatory Phenotype

scholarly journals Specialized subsets of innate-like T cells and dendritic cells protect from lethal pneumococcal infection in the lung

2021 ◽  
Author(s):  
Mallory Paynich Murray ◽  
Catherine M. Crosby ◽  
Paola Marcovecchio ◽  
Nadine Hartmann ◽  
Shilpi Chandra ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
T Cell Activation ◽  
Cell Activation ◽  
Pneumococcal Infection ◽  
Γδ T Cells ◽  
Cellular Interactions ◽  
Gm Csf ◽  
Mait Cells ◽  
Barrier Tissues

Innate-like T cells, including invariant natural killer T (iNKT) cells, mucosal-associated invariant T (MAIT) cells and γδ T cells, are present in various barrier tissues, including the lung. They carry out protective responses during infections, but the mechanisms for protection are not completely understood. Here, we investigated their roles during pulmonary infection with Streptococcus pneumoniae. Following infection, innate-like T cells rapidly increased in lung tissue, in part through recruitment, but TCR activation and cytokine production occurred mostly in IL-17-producing NKT17 and γδ T cells. NKT17 cells were preferentially located outside the vasculature prior to infection, as were CD103+ dendritic cells (cDC1), which were important both for antigen presentation to NKT17 cells and γδ T cell activation. Whereas IL-17A-producing γδ T cells also were numerous, GM-CSF was exclusive to NKT17 cells and contributed to iNKT cell-mediated protection. These studies demonstrate how particular cellular interactions and responses of functional subsets of innate-like T cells contribute to protection from pathogenic lung infection.

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