scholarly journals Free Energy Contribution to Gas Chromatographic Separation of Petroselinate and Oleate Esters

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Chanida Sansa-ard ◽  
Kornkanok Aryusuk ◽  
Supathra Lilitchan ◽  
Kanit Krisnangkura
Keyword(s):  
Fatty Acids ◽  
Molecular Weight ◽  
Gas Chromatography ◽  
Driving Force ◽  
Capillary Column ◽  
Free Energies ◽  
Energy Contribution ◽  
Free Energy Contribution ◽  
Gas Chromatographic Separation ◽  
Different Parts

The ease of separation by gas chromatography between petroselinic and oleic acids depends on the alcohol moieties of their esters. The esters of higher molecular weight alcohols tend to be better separated on a 90%-biscyanopropyl-10%-cyanopropylphenyl polysiloxane capillary column (30 m × 0.25 mm i.d.). By analysis of free energies contribution from different parts of the molecules, it is tentatively concluded that the interaction between the double bond and the column stationary phase is interfered by the bulky alkyl group, and it is the major driving force for the separation of the two fatty acids.

scholarly journals Antioxidant, Antimicrobial Activities and Fatty Acid Components of Flower, Leaf, Stem and Seed of Hypericum scabrum

2011 ◽  
Vol 6 (11) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Ali Shafaghat
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Hexane Extracts ◽  
Hypericum Scabrum ◽  
Different Parts

The hexane extracts of flower, leaf, stem, and seed of Hypericum scabrum, which were collected from northwestern Iran, were obtained by extraction in a Soxhlet apparatus. The fatty acids were converted to methyl esters and determined by gas chromatography/flame ionization detector (GC/FID) and gas chromatography/mass spectrometry (GC/MS) systems. The hexane extract from the flower, leaf, stem, and seed contained 39.1%, 43.2%, 29.0%, and 37.6% of omega-3 fatty acids, respectively. The other main components of the flower extract were tetracosane (12.2%) and palmitic acid (9.3%), and that of the leaf extract was palmitic acid (7.4%). The stem and seed extracts contained bis(2-ethylhexyl)phthalate (18.7% and 35.7%), nonacosane (11.7% and 3.9%) and linoleic acid (6.5% and 6.9%) as major components. The hexane extracts of different parts from H. scabrum represent an important source of omega-3 fatty acids in several Hypericum species. The antioxidant activity of all hexane extracts was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. The results indicate that hexane extracts from different parts of H. scabrum possess considerable antioxidant activity. The highest radical scavenging activity was detected in seed, which had an IC50 = 165 μg/mL. The antimicrobial activity of the extracts of those samples were determined against seven Gram-positive and Gram-negative bacteria ( Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, S. epidermidis, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae), as well as three fungi ( Candida albicans, Saccharomyces cerevisiae, and Aspergillus niger). The bioassay showed that the oil exhibited moderate antimicrobial activity. This study reveals that the all parts of this plant are attractive sources of fatty acid components, especially the essential ones, as well as of effective natural antioxidants.

Determination of Fatty Acids of Lower Molecular Weight by Gas Chromatography

Nature ◽  
1960 ◽  
Vol 187 (4738) ◽  
pp. 689-689 ◽  
Author(s):  
MARIE L. VORBECK ◽  
LEONARD R. MATTICK ◽  
FRANK A. LEE ◽  
CARL S. PEDERSON
Keyword(s):  
Fatty Acids ◽  
Molecular Weight ◽  
Gas Chromatography ◽  
Lower Molecular Weight

scholarly journals Extraction and Characterization of Sugarcane Peel Wax

ISRN Agronomy ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Mangesh B. Inarkar ◽  
S. S. Lele
Keyword(s):  
Fatty Acids ◽  
Molecular Weight ◽  
Gas Chromatography ◽  
Fourier Transform ◽  
Dry Weight ◽  
Weight Basis ◽  
Ft Ir ◽  
Gas Chromatography Mass Spectroscopy ◽  
Layer Chromatography

Sugarcane peel is an agrowaste product and contains considerable amount of wax. This has a good technoeconomic potential. In view of this, the present study aims at extraction and characterization of wax from sugarcane peel. The yield of crude wax was 0.95% on dry weight basis. During Fourier transform-infrared spectroscopy (FT-IR) prominent peaks obtained at 2921.73 and 2851.64 (–CH), 1463.44 (–CH2), 1376.96 (–CH3), 1108.4 and 1170.16 (–C–O) 3395.60 (–OH), 1710.25 (–CHO), and 1736.63 (–COOH) indicate presence of alkanes, ketones, alcohols, aldehydes, and carboxylic acids, respectively. Alcohol and hydrocarbon fractions were also found by thin layer chromatography (TLC). Melting point of crude wax was observed to be 62.1°C. Molecular weight of wax was estimated to be 1706 Dalton. Composition of crude wax found using gas chromatography-mass spectroscopy (GC-MS) was alkanes (28.83%), ester (66.26%), fatty acids (4.58%), aldehyde (0.11%), and alcohol (0.22%).

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