scholarly journals Ovine fetuses from slaughterhouses: A useful source for neural cell primary cultures

2021 ◽  
Author(s):  
Vittorio Farina ◽  
Sergio Domenico Gadau ◽  
Gianluca Lepore ◽  
Marcella Carcupino ◽  
Marco Zedda
Keyword(s):  
In Vitro Model ◽  
Primary Cultures ◽  
Medical Procedures ◽  
Cell Type ◽  
Experimental Animals ◽  
Vitro Model ◽  
Set Up ◽  
Very High ◽  
Β Tubulin

A lot of evidence demonstrates that sheep could represent an experimental model to set up medical procedures in view of their application on humans. Sheep are chosen as models for human biomechanical studies because their skeleton has some similarities to humans. The aim of this work was to set up sheep primary cultures from ovine fetuses at different ages, from pregnant uteri retrieved at local abattoirs. Cell characterization showed that one cell population was immunopositive to GFAP and identifiable as astrocytes, whereas a second cell type was III β-tubulin-positive, and hence classified as neurons. At 60-day old fetus is suitable to obtain neurons, whereas in a 90-day old fetus the cell culture is predominantly characterized by glial cells. The procedure here proposed is inexpensive, in fact, collecting fetuses during sheep slaughtering is a cost-saving option, unlike common experimental animals such as mice, rats, rabbits, that require very high economical efforts. Finally, our protocol fully eliminates the need of animal killing, being living animals replaced by a validated in vitro model in agreement with the 3Rs statement.

scholarly journals Ovine fetuses from slaughterhouses: a useful source for neural cell primary cultures

2019 ◽  
Author(s):  
Vittorio Farina ◽  
Sergio Domenico Gadau ◽  
Gianluca Lepore ◽  
Marcella Carcupino ◽  
Marco Zedda
Keyword(s):  
In Vitro Model ◽  
Primary Cultures ◽  
Medical Procedures ◽  
Cell Type ◽  
Experimental Animals ◽  
Vitro Model ◽  
Set Up ◽  
Cortical Regions ◽  
Very High

AbstractA lot of evidence demonstrates that sheep could represent an experimental model to set up medical procedures in a view of their application on humans. Sheep are chosen as model for human biomechanical studies because their skeleton has some similarities with humans. Sheep are gyrencephalic so the cerebral cortex can show valuable signposts to identify particular cortical regions, unlike rats that are lissencephalic animals. The aim of this work was to set up sheep primary cultures from ovine fetuses at different ages, from pregnant uteri retrieved at local abattoirs. Cell characterization demonstrated that one cell population was immunopositive to GFAP and identifiable as astrocytes, whereas a second cell type was III β-tubulin-positive, and hence classified as neurons. A 60 day old fetus is suitable to obtain neurons, whereas in a 90 day old fetus the cell culture is predominantly characterized by glial cells. The procedure here proposed is inexpensive. Indeed, the fetuses casually found during sheep slaughtering have no cost, unlike the classical experimental animals, such as mice, rats, rabbits, that require very high economical efforts. Finally, our protocol fully eliminates the need of animal killing, being living animals replaced by a validated in vitro model in agreement with the 3Rs statement.

The Human Leukemic HL-60 Cell Line: An in Vitro Model for Cell Death Endpoint Identification

1996 ◽  
Vol 24 (4) ◽  
pp. 581-587
Author(s):  
Cristiana Zanetti ◽  
Arrnalaura Stammati ◽  
Orazio Sapora ◽  
Flavia Zucco
Keyword(s):  
Cell Death ◽  
Cell Line ◽  
In Vitro Model ◽  
Leukemia Cell ◽  
Promyelocytic Leukemia ◽  
Leukemia Cell Line ◽  
Cell Type ◽  
Vitro Model ◽  
Promyelocytic Leukemia Cell Line

The aim of this study was to investigate the endpoints related to cell death, either necrosis or apoptosis, induced by four chemicals in the promyelocytic leukemia cell line, HL-60. Cell morphology, DNA fragmentation, cytofluorimetric analysis and oxygen consumption were used to classify the type of cell death observed. In our analysis, we found that not all the selected parameters reproduced the differences observed in the cell death caused by the four chemicals tested. As cell death is a very complex phenomenon, several factors should be taken into account (cell type, exposure time and chemical concentration), if chemicals are to be classified according to differences in the mechanisms more directly involved in cell death.

scholarly journals An in vitro feasibility investigation considering primary human melanocytes for spray- grafting of freshly isolated autologous skin cells for burn treatment and a clinical case report

2019 ◽  
pp. 1-8
Author(s):  
Jörg C. Gerlach ◽  
C. Johnen ◽  
B. Hartmann ◽  
J. Plettig ◽  
K. Bräutigam ◽  
...  
Keyword(s):  
Cell Morphology ◽  
Skin Color ◽  
In Vitro Model ◽  
Primary Cultures ◽  
Cell Suspensions ◽  
Epidermal Keratinocytes ◽  
Cell Counts ◽  
Skin Coloration ◽  
Vitro Model

A skin cell-spray grafting technique that enables the on-site application of freshly isolated autologous single cell suspensions was already applied in many cases on caucasian patients with low skin coloration. Our project hypothesis is that these suspensions contain keratinocytes and vital melanocytes, that are of particular interest for the treatment of patients of darker skin color. To test this, we applied an in vitro model, wherein the feasibility of i) isolating and ii) spraying of freshly isolated autologous melanocyte-keratinocyte cell suspensions was investigated. Primary human epidermal keratinocytes (HEKs) and melanocytes (MCs) were isolated from skin biopsies (n=8). Biochemical parameter, cell counts, cell morphology, growth behavior and immunofluorescence results were compared in two groups using MC cultures and co-cultures of MCs with HEKs. Case information on using the method clinically with one patient is included. The sprayed mixed cell suspensions proliferated in all groups without measurable loss of viability, and MCs exhibited a regular cell morphology in monoculture up to passage 4°. The sprayed MCs and HEKs demonstrated in vitro glucose and lactate metabolism that was comparable to the pipetted controls. In co-culture, well distributed CK14+ HEKs and NKI/beteb+ MCs could be demonstrated, which interacted in the in vitro model. The ratio of HEKs : MCs in our primary cultures were microscopically counted (n=8 each) as mean +/- SD 1,211,000 (+/- 574,343) HEK : 99,625 (+/- 59,025) MC; i.e., a ratio of approx. 12 : 1. Using the isolation method clinically for a patient with dark skin coloration after suffering severe second-degree burns shows a satisfying re-pigmentation of the resulting wound post healing. Freshly isolated spray-on melanocyte/keratinocyte suspensions provide for a considerable amount of viable HEKs and MCs. Using MCs in spray-grafting suspensions could represent a promising approach for treating severe partial-thickness burns and innovative therapy developments that also aim to address cosmetic aspects.

scholarly journals Atlantic salmon cardiac primary cultures: An in vitro model to study viral host pathogen interactions and pathogenesis

PLoS ONE ◽  
2017 ◽  
Vol 12 (7) ◽  
pp. e0181058 ◽  
Author(s):  
Patricia A. Noguera ◽  
Bianka Grunow ◽  
Matthias Klinger ◽  
Katherine Lester ◽  
Bertrand Collet ◽  
...  
Keyword(s):  
Atlantic Salmon ◽  
In Vitro Model ◽  
Primary Cultures ◽  
Host Pathogen Interactions ◽  
Host Pathogen ◽  
Vitro Model

scholarly journals Developing an in vitro model of T cell type of large granular lymphocyte leukemia

2013 ◽  
Vol 37 (12) ◽  
pp. 1737-1743 ◽  
Author(s):  
Tong Ren ◽  
Jun Yang ◽  
Katie Broeg ◽  
Xin Liu ◽  
Thomas P. Loughran ◽  
...  
Keyword(s):  
T Cell ◽  
In Vitro Model ◽  
Large Granular Lymphocyte ◽  
Cell Type ◽  
Large Granular Lymphocyte Leukemia ◽  
Vitro Model

scholarly journals Permeability properties of a three-cell type in vitro model of blood-brain barrier

2005 ◽  
Vol 9 (2) ◽  
pp. 373-379 ◽  
Author(s):  
Gabriella Schiera ◽  
Sandra Sala ◽  
Alessia Gallo ◽  
Maria Pia Raffa ◽  
Giovanna Laura Pitarresi ◽  
...  
Keyword(s):  
Blood Brain Barrier ◽  
In Vitro Model ◽  
Brain Barrier ◽  
Cell Type ◽  
Blood Brain ◽  
Vitro Model

scholarly journals Development of a Custom Extracorporeal Circuit for Endovascular Resuscitation Research

2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Jonathan J Morrison ◽  
Hossam Abdou ◽  
Michael Richmond ◽  
Marta J Madurska ◽  
Noha Elansary
Keyword(s):  
Aortic Root ◽  
In Vitro Model ◽  
Swine Model ◽  
Peristaltic Pump ◽  
Extracorporeal Circuit ◽  
Extracorporeal Circuits ◽  
Vitro Model ◽  
Aortic Cannula ◽  
Set Up

Background: To demonstrate the utility and applicability of in vitro extracorporeal circuits in endovascular resuscitation research. Methods: The method for building an inexpensive in vitro extracorporeal circuit for endovascular resuscitation research is described. In this study, aortic cannulas and pump combinations were evaluated in the in vitro extracorporeal circuit. Then one aortic cannula and pump set up was evaluated in a post-mortem swine model. Flow data was collected and compared among groups. Results: The peristaltic pump generated the highest flow as compared to the other pump combinations at any given catheter size. The peristaltic pump combined with the 10 Fr cannula produced the highest flow overall at 2304 mL/min. This same combination produced a peak flow of 886 ml/min at the aortic root in the swine model. Conclusions: The flow generated in the swine model was less than half of that generated in the in vitro model. However, all flow was channeled through one outflow tract in the in vitro model whereas the swine aorta has several branches of outflow. As such, a 50% reduction in flow or greater is anticipated at the level of the aortic root. An in vitro extracorporeal circuit for endovascular research can be built for less than $10,000, with most of the materials being reusable, and can be used to generate representative data that may be anticipated in a swine model.  

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