scholarly journals Quantum dots nanoparticle-based lateral flow assay for rapid detection of Mycobacterium species using anti-FprA antibodies

2012 ◽  
Vol 2 (1) ◽  
pp. 5 ◽  
Author(s):  
Fabio Cimaglia ◽  
Alessandro Aliverti ◽  
Maurizio Chiesa ◽  
Palmiro Poltronieri ◽  
Enrico De Lorenzis ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Quantum Dots ◽  
Monoclonal Antibodies ◽  
Rapid Detection ◽  
Detection System ◽  
Lateral Flow ◽  
Fluorescence Signal ◽  
Human Pathogens ◽  
Detection Zone ◽  
Diagnostic Investigation

A lateral flow (LF) device combined with quantum dots (QDs) technology was developed for rapid detection of a specific mycobacterial flavoprotein reductase (<em>fprA</em>). In order to develop the LF assay based on a double-antibody sandwich format, two monoclonal antibodies recognizing different epitopes located in separated <em>fprA</em> domains were identified. The first monoclonal antibody was immobilized onto the detection zone of a porous nitrocellulose membrane, whereas another monoclonal antibody was conjugated to QDs nanoparticles as a detection system. Using these monoclonal antibodies we recorded a good fluorescence signal, the intensity of which was directly proportional to the concentration of <em>fprA</em> protein. The use of antibodies conjugated with fluorescent semiconductor QDs via biotin-streptavidin bridge, allowed the detection of <em>fprA</em> protein at concentrations as low as 12.5 pg/μL in less than 10 min. The reported technology could be useful in the diagnostic investigation of <em>Mycobacterium tuberculosis</em> and other human pathogens in clinical specimens.

Rapid detection of Shiga toxin type II using lateral flow immunochromatography test strips of colorimetry and fluorimetry

The Analyst ◽  
2020 ◽  
Vol 145 (1) ◽  
pp. 76-82 ◽  
Author(s):  
Tian Lu ◽  
Kai-Di Zhu ◽  
Chao Huang ◽  
Tian Wen ◽  
Yong-Jun Jiao ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Gold Nanoparticles ◽  
Shiga Toxin ◽  
Rapid Detection ◽  
Lateral Flow ◽  
Cdte Quantum Dots ◽  
Type Ii ◽  
Immunochromatographic Test ◽  
Test Strips

Two types of lateral flow immunochromatographic test strips (LFITS) using gold nanoparticles and fluorescent CdTe quantum dots (QDs) as signal labels, respectively, were developed for Shiga toxin type II (STX2) assays.

Monoclonal antibody-based solid-phase immunoenzymometric assays for quantifying human immunoglobulin G and its subclasses in serum.

1985 ◽  
Vol 31 (12) ◽  
pp. 1940-1945 ◽  
Author(s):  
C Papadea ◽  
I J Check ◽  
C B Reimer
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Solid Phase ◽  
Detection System ◽  
Reference Intervals ◽  
Microtiter Plates ◽  
Myeloma Proteins ◽  
Human Proteins ◽  
Reference Preparation ◽  
Apparently Healthy

Abstract We developed quantitative immunoenzymometric assays for human IgG and its subclasses by using monoclonal antibodies, an avidin-biotin detection system and, as the calibrant, the U.S. National Reference Preparation for Specific Human Proteins. The assays are sensitive (detecting as little as 6 micrograms/L), precise (average inter-assay CV less than 11%), and vary linearly with concentrations over a five- to 10-fold range, depending on the monoclonal antibody. We evaluated 22 different monoclonal antibodies, many of which remained highly reactive when immobilized in wells of microtiter plates coated with bovine serum albumin-glutaraldehyde to "capture" total IgG or subclasses of IgG in the sample. We demonstrated the specificity of the most reactive antibodies by using a panel of 20 purified myeloma proteins. The sum of IgG subclass concentrations correlated well (r = 0.84, p less than 0.001) with the total IgG measured in sera from 63 apparently healthy adults (26 men, 37 women). We estimated 95 percentile reference intervals for the immunoglobulins in these subjects and determined the following mean percentage distributions of IgG subclasses: IgG1 49, IgG2 33, IgG3 9, and IgG4 7. The availability of these assays should facilitate studies of the clinical significance of the subclasses.

Self-paired monoclonal antibody lateral flow immunoassay strip for rapid detection of Acidovorax avenae subsp. citrulli

2016 ◽  
Vol 408 (22) ◽  
pp. 6071-6078 ◽  
Author(s):  
Haijuan Zeng ◽  
Wenbo Guo ◽  
Beibei Liang ◽  
Jianwu Li ◽  
Xuzhao Zhai ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Rapid Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay

scholarly journals Development of a fluorescent immunochromatographic assay based on quantum dots for the detection of fleroxacin

RSC Advances ◽  
2021 ◽  
Vol 11 (36) ◽  
pp. 22005-22013
Author(s):  
Qingbao Yang ◽  
Yanhua Qi ◽  
Jingming Zhou ◽  
Yumei Chen ◽  
Chao Liang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Quantum Dots ◽  
Fluorescent Probe ◽  
Rapid Detection ◽  
Detection Method ◽  
Water Soluble ◽  
Fluorescent Detection ◽  
Immunochromatographic Assay

(1) Water-soluble CdSe/ZnS QDs and an anti-FLE monoclonal antibody were used to prepare a fluorescent probe. (2) Primary rapid detection of FLE residues with visual fluorescent detection method.

scholarly journals A new fluorescent detection system for identifying variant hemoglobins after gel electrophoresis using immunobinding with monoclonal antibodies.

1986 ◽  
Vol 34 (5) ◽  
pp. 585-591 ◽  
Author(s):  
R E Smith
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Gel Electrophoresis ◽  
Detection System ◽  
Great Sensitivity ◽  
Fluorescent Detection ◽  
Fluorogenic Substrate ◽  
Allelic Variants ◽  
Resolution System ◽  
Entire Procedure

This paper describes a low-resolution system for identifying variant hemoglobins with great sensitivity and specificity. After electrophoresis of the hemoglobin sample in a gel, fixation is used to entrap the hemoglobin. The gel is dried, incubated with a monoclonal antibody against the desired hemoglobin, then incubated with a second antibody against the first antibody which is conjugated with the enzyme beta-d-galactosidase. An enzyme overlay membrane containing a fluorogenic substrate is then placed on the gel surface, incubated, and removed, yielding an immunofluorescent print. The entire procedure takes only two hours, and by virtue of fluorescent detection gives sharper band resolution and greater sensitivity than conventional dye methods. The system clearly distinguishes SS sickle-cell hemoglobin from heterozygous and "S-like" hemoglobins. The technique therefore holds promise as a powerful probe for allelic variants.

Development of Enzyme linked Immunosorbent Assay and Lateral Flow Immunoassay for the Rapid Detection of Dapsone in Foods

2021 ◽  
Author(s):  
Chuanlai Xu ◽  
xin guo ◽  
lu lin ◽  
Shanshan Song ◽  
aihong wu ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Human Health ◽  
Immunosorbent Assay ◽  
Food Chain ◽  
Rapid Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Food Animals

The abuse of dapsone (DDS) in food animals could result in its accumulation in humans through the food chain thus endangering human health. A sensitive monoclonal antibody (mAb) against DDS...

scholarly journals Nucleic Acid-Based Lateral Flow Biosensor for Salmonella Typhi and Salmonella Paratyphi: A Detection in Stool Samples of Suspected Carriers

Diagnostics ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 700
Author(s):  
Zulkiply Nor Amalina ◽  
Muhammad Fazli Khalid ◽  
Sjafri Faizul Rahman ◽  
Muhamad Nuramin Ahmad ◽  
Mohamad Ahmad Najib ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Rapid Detection ◽  
Detection System ◽  
Flow Analysis ◽  
Fluorescein Isothiocyanate ◽  
Salmonella Typhi ◽  
Lateral Flow ◽  
Surveillance Program ◽  
Biotin Binding ◽  
Stool Samples

A multiplex rapid detection system, based on a PCR-lateral flow biosensor (mPCR-LFB) was developed to identify Salmonella Typhi and Salmonella Paratyphi A from suspected carriers. The lower detection limit for S. Typhi and S. Paratyphi A was 0.16 and 0.08 ng DNA equivalent to 10 and 102 CFU/mL, respectively. Lateral flow biosensor was used for visual detection of mPCR amplicons (stgA, SPAint, ompC, internal amplification control) by labeling forward primers with fluorescein-isothiocyanate (FITC), Texas Red, dinitrophenol (DNP) and digoxigenin (DIG) and reverse primers with biotin. Binding of streptavidin-colloidal gold conjugate with the amplicons resulted in formation of a red color dots on the strip after 15–20 min of sample exposure. The nucleic acid lateral flow analysis of the mPCR-LFB was better in sensitivity and more rapid than the conventional agarose gel electrophoresis. Moreover, the mPCR-LFB showed 100% sensitivity and specificity when evaluated with stools spiked with 100 isolates of Salmonella genus and other bacteria. A prospective cohort study on stool samples of 1176 food handlers in outbreak areas (suspected carriers) resulted in 23 (2%) positive for S. Typhi. The developed assay has potential to be used for rapid detection of typhoid carriers in surveillance program.

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