Self-paired monoclonal antibody lateral flow immunoassay strip for rapid detection of Acidovorax avenae subsp. citrulli

The abuse of dapsone (DDS) in food animals could result in its accumulation in humans through the food chain thus endangering human health. A sensitive monoclonal antibody (mAb) against DDS...

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An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

SLAS TECHNOLOGY Translating Life Sciences Innovation ◽

10.1177/2472630320981935 ◽

2021 ◽

pp. 247263032098193

Author(s):

Cheng Liu ◽

Shuiqin Fang ◽

Yachen Tian ◽

Youxue Wu ◽

Meijiao Wu ◽

...

Keyword(s):

Escherichia Coli ◽

Rapid Detection ◽

Foodborne Pathogen ◽

Test Strip ◽

Lateral Flow ◽

Detection Sensitivity ◽

Lateral Flow Immunoassay ◽

Escherichia Coli O157 ◽

Aggregation Induced Emission ◽

E Coli

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.

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A paper-based competitive lateral flow immunoassay for multi β-agonist residues by using a single monoclonal antibody labelled with red fluorescent nanoparticles

Microchimica Acta ◽

10.1007/s00604-018-2730-9 ◽

2018 ◽

Vol 185 (3) ◽

Cited By ~ 14

Author(s):

Ruiguo Wang ◽

Wei Zhang ◽

Peilong Wang ◽

Xiaoou Su

Keyword(s):

Monoclonal Antibody ◽

Lateral Flow ◽

Lateral Flow Immunoassay ◽

Fluorescent Nanoparticles

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Development of a Prototype Lateral Flow Immunoassay for Rapid Detection of Staphylococcal Protein A in Positive Blood Culture Samples

Diagnostics ◽

10.3390/diagnostics10100794 ◽

2020 ◽

Vol 10 (10) ◽

pp. 794

Author(s):

Arpasiri Srisrattakarn ◽

Patcharaporn Tippayawat ◽

Aroonwadee Chanawong ◽

Ratree Tavichakorntrakool ◽

Jureerut Daduang ◽

...

Keyword(s):

Blood Culture ◽

Positive Blood Culture ◽

Rapid Detection ◽

Protein A ◽

Lateral Flow ◽

Lateral Flow Immunoassay ◽

Staphylococcal Protein A ◽

Highly Sensitive ◽

Sophisticated Equipment ◽

Higher Sensitivity

Bloodstream infection (BSI) is a major cause of mortality in hospitalized patients worldwide. Staphylococcus aureus is one of the most common pathogens found in BSI. The conventional workflow is time consuming. Therefore, we developed a lateral flow immunoassay (LFIA) for rapid detection of S. aureus-protein A in positive blood culture samples. A total of 90 clinical isolates including 58 S. aureus and 32 non-S. aureus were spiked in simulated blood samples. The antigens were extracted by a simple boiling method and diluted before being tested using the developed LFIA strips. The results were readable by naked eye within 15 min. The sensitivity of the developed LFIA was 87.9% (51/58) and the specificity was 93.8% (30/32). When bacterial colonies were used in the test, the LFIA provided higher sensitivity and specificity (94.8% and 100%, respectively). The detection limit of the LFIA was 107 CFU/mL. Initial evaluation of the LFIA in 20 positive blood culture bottles from hospitals showed 95% agreement with the routine methods. The LFIA is a rapid, simple and highly sensitive method. No sophisticated equipment is required. It has potential for routine detection particularly in low resource settings, contributing an early diagnosis that facilitates effective treatment and reduces disease progression.

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Quantum dots nanoparticle-based lateral flow assay for rapid detection of Mycobacterium species using anti-FprA antibodies

Nanotechnology Development ◽

10.4081/nd.2012.e5 ◽

2012 ◽

Vol 2 (1) ◽

pp. 5 ◽

Cited By ~ 7

Author(s):

Fabio Cimaglia ◽

Alessandro Aliverti ◽

Maurizio Chiesa ◽

Palmiro Poltronieri ◽

Enrico De Lorenzis ◽

...

Keyword(s):

Monoclonal Antibody ◽

Quantum Dots ◽

Monoclonal Antibodies ◽

Rapid Detection ◽

Detection System ◽

Lateral Flow ◽

Fluorescence Signal ◽

Human Pathogens ◽

Detection Zone ◽

Diagnostic Investigation

A lateral flow (LF) device combined with quantum dots (QDs) technology was developed for rapid detection of a specific mycobacterial flavoprotein reductase (fprA). In order to develop the LF assay based on a double-antibody sandwich format, two monoclonal antibodies recognizing different epitopes located in separated fprA domains were identified. The first monoclonal antibody was immobilized onto the detection zone of a porous nitrocellulose membrane, whereas another monoclonal antibody was conjugated to QDs nanoparticles as a detection system. Using these monoclonal antibodies we recorded a good fluorescence signal, the intensity of which was directly proportional to the concentration of fprA protein. The use of antibodies conjugated with fluorescent semiconductor QDs via biotin-streptavidin bridge, allowed the detection of fprA protein at concentrations as low as 12.5 pg/μL in less than 10 min. The reported technology could be useful in the diagnostic investigation of Mycobacterium tuberculosis and other human pathogens in clinical specimens.

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