scholarly journals Effects of Bt maize on the reproduction and development of the freshwater snail Bulinus tropicus

2012 ◽  
Vol 31 (1) ◽  
Author(s):  
K. Minnaar ◽  
H. Bouwman
Keyword(s):  
Growth Rate ◽  
Fresh Water ◽  
Agricultural Land ◽  
Genetically Modified ◽  
Genetically Modified Crops ◽  
Freshwater Snail ◽  
Bt Maize

This study was conducted to investigate the effects of genetically modified crops onaquatic environments around agricultural land. Fresh water snails indicated effects duringembryonic development, and on the growth rate of survivors.

scholarly journals Optimisation of qualitative and semi-quantitative detection of genetically modified crops by PCR

2009 ◽  
Vol 57 (5) ◽  
pp. 313-318
Author(s):  
Tomáš Vyhnánek ◽  
Pavel Hanáček
Keyword(s):  
Quantitative Pcr ◽  
Genetically Modified ◽  
Genetically Modified Crops ◽  
Quantitative Detection ◽  
Isogenic Line ◽  
Maize Genome ◽  
Bt Maize ◽  
Qualitative Detection ◽  
Maize Mon810 ◽  
Semi Quantitative Pcr

For qualitative and semi-quantitative detection of genetically modified crops we selected the detection of the frequently used promoter 35S CaMV. To optimise the method we used two commercially available genotypes of maize from the company Monsanto (USA), i.e. the transgenic hybrid Bt-maize line MON810 and a genetically non-modified control (isogenic line to MON810). We tested the pri­mers and PCR programmes described by Greiner et al. (2005) and Hernandéz et al. (2005). When applying PCR methods of detection of Bt-maize the first step was to optimise the protocol for the detection of the maize genome and detection of the specific sites of genetically modified MON810 maize. For detection of the maize genome we selected the primers IVR1-F and IVR1-R (invertase gene) which verify the presence of the maize genome by a 226 bp product. For qualitative detection of the insert of Bt-maize MON810 the primer pairs VW01/VW03 (Greiner et al., 2005) and BT03/BT04 (Hernandéz et al., 2005) were used to detect the 35S CaMV promoter. Products of the size 178 bp and 280 bp, respectively, verify its presence. Based on the results of qualitative PCR we selected the primers VW01/VW03 for semi-quantitative detection of the amount of DNA of Bt-maize. For semi-quantitative PCR we have chosen sampling of the amplification product in the 30th cycle of the PCR reaction. In the genetically unmodified control a detection limit of 1% of admixture of Bt-maize was determined when using semi-quantitative PCR. The same primers as for semi-quantitative PCR were also used for multiplex PCR but with half the concentration of primers for standard PCR. This protocol however will have to be further optimised. The presented results introduce PCR methods for qualitative and semi-quantitative detection of DNA of the genetically modified Bt-maize MON810 which can also be used for other GM crops containing the 35S CaMV promoter. It could be suitable to use these methods for the qualitative detection and/or for screening analyses of the detection of successfulness of transformation experiments.

Greening Gulf Landscapes

2018 ◽  
pp. 99-120 ◽  
Author(s):  
Ali El-Keblawy
Keyword(s):  
Water Resources ◽  
Fresh Water ◽  
Abiotic Stresses ◽  
Genetically Modified ◽  
Genetically Modified Crops ◽  
Agricultural Lands ◽  
Natural Habitats ◽  
Tolerant Plants ◽  
Local Environments ◽  
Gulf States

Gulf states have paid too much effort toward the greening of cities and too little to fundamentally changing the way they see agricultural lands and deteriorated natural habitats. Most of the greening programs prioritise techniques and interventions, such as introducing exotic plants, that are not adapted to the local environments. The result is that greening projects, especially those in deserts, have failed and proven unsustainable. This chapter discuss what the alternative -maintaining sustainable green desert landscapes- might look like. Proposed innovations include using native trees, instead of exotics, and introducing genetically modified crops designed to tolerate the abiotic stresses and salt tolerant plants (halophytes) to reduce the pressure on fresh water resources

scholarly journals Monarch butterfly and milkweed declines substantially predate the use of genetically modified crops

2019 ◽  
Vol 116 (8) ◽  
pp. 3006-3011 ◽  
Author(s):  
J. H. Boyle ◽  
H. J. Dalgleish ◽  
J. R. Puzey
Keyword(s):  
Genetically Modified ◽  
Danaus Plexippus ◽  
Genetically Modified Crops ◽  
Monarch Butterfly ◽  
The United States ◽  
Gm Crops ◽  
Herbicide Resistant ◽  
The Past ◽  
Scientific Attention ◽  
Herbicide Use

Monarch butterfly (Danaus plexippus) decline over the past 25 years has received considerable public and scientific attention, in large part because its decline, and that of its milkweed (Asclepias spp.) host plant, have been linked to genetically modified (GM) crops and associated herbicide use. Here, we use museum and herbaria specimens to extend our knowledge of the dynamics of both monarchs and milkweeds in the United States to more than a century, from 1900 to 2016. We show that both monarchs and milkweeds increased during the early 20th century and that recent declines are actually part of a much longer-term decline in both monarchs and milkweed beginning around 1950. Herbicide-resistant crops, therefore, are clearly not the only culprit and, likely, not even the primary culprit: Not only did monarch and milkweed declines begin decades before GM crops were introduced, but other variables, particularly a decline in the number of farms, predict common milkweed trends more strongly over the period studied here.

Environment and its Forms of Knowledge: The Regulation of Genetically Modified Crops in India

2021 ◽  
Vol 37 (2) ◽  
pp. 167-183
Author(s):  
Aniket Aga
Keyword(s):  
Power Relations ◽  
Genetically Modified ◽  
Genetically Modified Crops ◽  
Gm Crops ◽  
Regulatory Regime ◽  
Democratic Participation ◽  
Environment Protection ◽  
Policy Decisions ◽  
Inherent Ambiguity ◽  
Forms Of Knowledge

A fierce controversy over genetically modified (GM) crops has been raging in India for over two decades. Analyzing India’s regulatory regime for GM crops, this article focuses on the modes through which state bureaucracies know the environment. It argues that two epistemologies - scientific and legal-administrative – underpin environment protection. By unraveling the course of regulatory disputes, I demonstrate that bureaucracies are not just hierarchically divided but are also segmented by horizontal, functional specializations. There is thus an inherent ambiguity lodged between environment as a technical discourse and as statecraft. This ambiguity both fosters and constrains democratic participation in policy decisions and can even partially disrupt power relations in unanticipated ways.

Sign in / Sign up

Export Citation Format

Share Document