Antimicrobial susceptibility pattern of vancomycin resistant enterococci to newer antimicrobial agents

The in vitro activities of 16 antimicrobial agents against 86 strains of Corynebacterium striatum were evaluated by microdilution using cation-adjusted Mueller-Hinton broth. MICs at which 90% of strains were inhibited were 0.06 microgram/ml for teicoplanin, 1 microgram/ml for vancomycin, 0.03 to 8 micrograms/ml for beta-lactams, 8 micrograms/ml for sparfloxacin, 16 micrograms/ml for ciprofloxacin, 16/304 micrograms/ml for co-trimoxazole (trimethoprim-sulfamethoxazole), 64 micrograms/ml for tetracycline, 128 micrograms/ml for gentamicin, and > 128 micrograms/ml for amikacin, erythromycin, and rifampin.

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Prevalence and antimicrobial susceptibility pattern of methicillin-resistant, vancomycin-resistant, and Panton-Valentine leukocidin positive Staphylococcus aureus in a tertiary care hospital Dhaka, Bangladesh

Tzu Chi Medical Journal ◽

10.1016/j.tcmj.2014.12.001 ◽

2015 ◽

Vol 27 (1) ◽

pp. 10-14 ◽

Cited By ~ 5

Author(s):

Tashmin Afroz Binte Islam ◽

S.M. Shamsuzzaman

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Susceptibility ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Care Hospital ◽

Susceptibility Pattern ◽

Antimicrobial Susceptibility Pattern ◽

Vancomycin Resistant ◽

Panton Valentine Leukocidin ◽

Valentine Leukocidin

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Plaquelike Clearings Induced by Antimicrobial Agents on Lawns of Pseudomonas aeruginosa

Infection and Immunity ◽

10.1128/iai.1.2.190-194.1970 ◽

1970 ◽

Vol 1 (2) ◽

pp. 190-194

Author(s):

Marian W. Wolfe ◽

Daniel Amsterdam

Keyword(s):

Pseudomonas Aeruginosa ◽

Antimicrobial Susceptibility ◽

Antimicrobial Agents ◽

Biochemical Properties ◽

Plaque Formation ◽

Previous Exposure ◽

Susceptibility Pattern ◽

Antimicrobial Susceptibility Pattern ◽

The Relationship

Plaques similar in appearance to those induced by phage were observed adjacent to chloramphenicol and tetracycline discs on Pseudomonas aeruginosa lawns used for the determination of antibiotic susceptibility. Thirteen strains were selected for study, 10 of which exhibited the plaquing phenomenon. The ability to form plaques induced by tetracycline was not related to any of the biochemical properties of the strains studied, their overall antimicrobial susceptibility pattern, or their pathological source. Some pseudomonad strains were capable of pyocin production; however, the relationship between plaque formation and pyocin production was not apparent. Supernatant fluids of resuspended plaque contents of eight strains originally demonstrating clearings could induce plaques on sensitive indicator lawns only when collected from tetracycline-induced plaque areas; supernatant fluids of the same strains could not produce clearings without previous exposure to the drug. Of the eight supernatant fluids capable of plaque induction, three were active on their homologous indicator lawns. In a subsequent survey of 95 P. aeruginosa strains, it was found that 28 isolates exhibited plaques. Of these, 17 were associated with tetracycline, 7 were associated with chloramphenicol, 3 were associated with triple sulfa; and 1 was associated with nalidixic acid.

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Antimicrobial Resistance Genes in Porcine Pasteurella multocida Are Not Associated with Its Antimicrobial Susceptibility Pattern

Antibiotics ◽

10.3390/antibiotics9090614 ◽

2020 ◽

Vol 9 (9) ◽

pp. 614

Author(s):

Máximo Petrocchi-Rilo ◽

César-B. Gutiérrez-Martín ◽

Esther Pérez-Fernández ◽

Anna Vilaró ◽

Lorenzo Fraile ◽

...

Keyword(s):

Resistance Genes ◽

Antimicrobial Susceptibility ◽

Pasteurella Multocida ◽

Antimicrobial Agents ◽

Tetracycline Resistance ◽

Susceptibility Pattern ◽

Antimicrobial Susceptibility Pattern ◽

Tetracycline Resistance Genes ◽

Antimicrobial Resistance Genes ◽

Western Spain

Forty-eight Pasteurella multocida isolates were recovered from porcine pneumonic lungs collected from farms in “Castilla y León” (north-western Spain) in 2017–2019. These isolates were characterized for their minimal inhibition concentrations to twelve antimicrobial agents and for the appearance of eight resistance genes: tetA, tetB, blaROB1, blaTEM, ermA, ermC, mphE and msrE. Relevant resistance percentages were shown against tetracyclines (52.1% for doxycycline, 68.7% for oxytetracycline), sulphamethoxazole/trimethoprim (43.7%) and tiamulin (25.0%), thus suggesting that P. multocida isolates were mostly susceptible to amoxicillin, ceftiofur, enrofloxacin, florfenicol, marbofloxacin and macrolides. Overall, 29.2% of isolates were resistant to more than two antimicrobials. The tetracycline resistance genes (tetA and tetB) were detected in 22.9% of the isolates, but none were positive to both simultaneously; blaROB1 and blaTEM genes were found in one third of isolates but both genes were detected simultaneously in only one isolate. The ermC gene was observed in 41.7% of isolates, a percentage that decreased to 22.9% for msrE; finally, ermA was harbored by 16.7% and mphE was not found in any of them. Six clusters were established based on hierarchical clustering analysis on antimicrobial susceptibility for the twelve antimicrobials. Generally, it was unable to foresee the antimicrobial susceptibility pattern for each family and the association of each particular isolate inside the clusters established from the presence or absence of the resistance genes analyzed.

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Antimicrobial Resistance Genes in Porcine Pasteurella Multocida are not Associated with its Antimicrobial Susceptibility Pattern

10.20944/preprints202009.0089.v1 ◽

2020 ◽

Author(s):

Máximo Patrocchi-Rilo ◽

César-B. Gutiérrez-Martín ◽

Esther Pérez-Fernández ◽

Anna Vilaró ◽

Lorenzo Fraile ◽

...

Keyword(s):

Resistance Genes ◽

Antimicrobial Susceptibility ◽

Clustering Analysis ◽

Pasteurella Multocida ◽

Antimicrobial Agents ◽

Tetracycline Resistance ◽

Susceptibility Pattern ◽

Antimicrobial Susceptibility Pattern ◽

Tetracycline Resistance Genes ◽

Antimicrobial Resistance Genes

Forty-eight Pasteurella multocida isolates were recovered from porcine pneumonic lungs collected in Norwestern Spain (2017- 2019). These isolates were characterized for their minimal inhibition concentrations to twelve antimicrobial agents and for the appearance of eight resistance genes: tetA, tetB, blaROB1, blaTEM, ermA, ermC, mphE and msrE. Relevant resistance percentages were shown to teracyclines, sulphamethoxazole/trimethoprim and tiamulin, thus suggesting that P. multocida isolates were mostly susceptible to amoxicillin, ceftiofur, enrofloxacin, florfenicol, marbofloxacin and macrolides. 29.2% of isolates were resistant to more than two antimicrobials. The tetracycline resistance genes (tetA and tetB) were detected in 22.9% of the isolates, but none was positive to both simultaneously; blaROB1 and blaTEM genes were found in one third of isolates but both genes were detected simultaneously in only one isolate. ermC gene was observed in 41.7% of isolates, a percentage that decreased until 22.9% for msrE; finally, ermA was harboured by 16.7% and mphE was not found in any of them. Six clusters were established based on hierarchical clustering analysis on antimicrobial susceptibility for the twelve antimicrobials. Generally, it was unable to foresee the antimicrobial susceptibility pattern for each family and the association of each particular isolate inside the clusters established from the presence or absence of the resistance genes analyzed.

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