Surgical pearl: Looped razor blade for excision surgery on concha and fossa of the ear

The advantages of freeze substitution fixation over conventional chemical fixation for preservation of ultrastructural details in fungi have been discussed by various authors. As most ascomycetes, basidiomycetes and deuteromycetes do not fix well using conventional chemical fixation protocols, freeze substitution has attracted the attention of many individuals interested in fungal ultrastructure. Thus far most workers using this technique on fungi have concentrated on thin walled somatic hyphae. However, in our laboratory we have experimented with the use of freeze substitution on a variety of fungal reproductive structures and spores with promising results.Here we present data on freeze substituted samples of sporangia of the zygomycete Umbellopsis vinacea, basidia of Exobasidium camelliae var. gracilis, developing teliospores of the smut Sporisorium sorghi, germinating teliospores of the rust Gymnosporangium clavipes, germinating conidia of the deuteromycete Cercosporidium personatum, and developing ascospores of Ascodesmis nigricans.Spores of G. clavipes and C. personatum were deposited on moist pieces of sterile dialysis membrane where they hydrated and germinated. Asci of A. nigricans developed on pieces of dialysis membrane lying on nutrient agar plates. U. vinacea was cultured on small pieces of agar-coated wire. In the plant pathogens E. camelliae var. gracilis and S. sorghi, a razor blade was used to remove smal1 pieces of infected host issue. All samples were plunged directly into liquid propane and processed for study according to Hoch.l Samples on dialysis membrane were flat embedded. Serial thin sections were cut using a diamond knife, collected on slot grids, and allowed to dry down onto Formvar coated aluminum racks. Sections were post stained with uranyl acetate and lead citrate.

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Effects of Mg2+ on Chromatic Structure in Isolated Chicken Liver Nuclei

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100158194 ◽

1990 ◽

Vol 48 (3) ◽

pp. 130-131

Author(s):

Soichiro Arai ◽

Yuh H. Nakanishi

Keyword(s):

Chromatin Structure ◽

Room Temperature ◽

Osmium Tetroxide ◽

Divalent Cations ◽

Chromatin Condensation ◽

Chicken Liver ◽

Electron Microscopic ◽

Razor Blade ◽

Microscopic Studies ◽

Liver Nuclei

Although many electron microscopic studies on extracted chromatin have provided considerable information on chromatin condensation induced by divalent cations, there is only a little literature available on the effects of divalent cations on chromatin structure in intact nuclei. In the present study, the effects of Mg2+ on chromatin structure in isolated chicken liver nuclei were examined over a wide concentration range of Mg2+ by scanning electron microscopy.Nuclei were prepared from chicken liver by the method of Chauveau et al. with some modifications. The nuclei were suspended in 25 mM triethanolamine chloride buffer (pH7.4) with 1 mM EDTA or in the buffer with concentrations of MgCl2 varying from 1 to 50 mM. After incubation for 1 min at 0°C, glutaraldehyde was added to 1.8% and the nuclei were fixed for 1 h at 4°C. The fixed nuclei were mixed with 15% gelatin solution warmed at about 40°C, and kept at room temperature until the mixture set. The gelatin containing the nuclei was fixed with 2% glutaraldehyde for 2-4 h, and cut into small blocks. The gelatin blocks were conductive-stained with 2% tannic acid and 2% osmium tetroxide, dehydrated in a graded series of ethanol, and freeze-cracked with a razor blade in liquid nitrogen.

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The Razor Blade in the Apple: The Social Construction of Urban Legends

Social Problems ◽

10.1525/sp.1985.32.5.03a00070 ◽

1985 ◽

Vol 32 (5) ◽

pp. 488-499 ◽

Cited By ~ 16

Author(s):

Joel Best ◽

Gerald T. Horiuchi

Keyword(s):

Social Construction ◽

Razor Blade ◽

Urban Legends ◽

The Social

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THE UBIQUITOUS RAZOR BLADE: A POSTSCRIPT

ARLIS/NA Newsletter ◽

10.1086/arlisnanews.2.6.27945336 ◽

1974 ◽

Vol 2 (6) ◽

pp. 96-96

Keyword(s):

Razor Blade

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The use of paquette blade handle in harvesting free gingival graft from the palate – a report of three cases with its method of use, advantages and disadvantages.

RGUHS Journal of Dental Sciences ◽

10.26715/rjds.10_2_6 ◽

2018 ◽

Vol 10 (2) ◽

pp. 32-38

Author(s):

Abhilash Neelakanti ◽

Abdul Hakeem ◽

Sunil Sathyanarayana

Keyword(s):

Surgical Procedure ◽

Hard Palate ◽

Root Coverage ◽

Razor Blade ◽

Ridge Augmentation ◽

Advantages And Disadvantages ◽

Free Gingival Graft ◽

Gingival Graft

Free gingival grafting is a well-known periodontal plastic surgical procedure used for gingival augmentation, for root coverage procedures and in some cases of pre-prosthetic ridge augmentation. Several instruments have been used to harvest the graft from hard palate, ranging from scalpel to mucotome, each of which has its own advantages and disadvantages. One such instrument is Paquette blade handle, which incorporates a regular razor blade for graft harvesting. This article intends to describe in detail the handling of this instrument, its usefulness as well as limitations, as explained in the following three cases.

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Stem Canker of Cabbage Seed Stalks Caused by Botrytis cinerea in Western Washington

Plant Disease ◽

10.1094/pdis.2001.85.5.559d ◽

2001 ◽

Vol 85 (5) ◽

pp. 559-559

Author(s):

D. Inglis ◽

M. Derie ◽

T. Hsiang

Keyword(s):

Brassica Oleracea ◽

Botrytis Cinerea ◽

Mechanical Damage ◽

Tween 80 ◽

Stem Canker ◽

The United States ◽

Gray Mold ◽

Freezing Injury ◽

Razor Blade ◽

Western Washington

Stem cankers were observed during 1998 on bolting stalks of cabbage (Brassica oleracea var. capitata L.) in seed production fields in western Washington. In 1999, approximately 4 ha of cabbage hybrid 'Wk 121, was severely affected. Lesions occurred at the base of seed stalks after they emerged from heads of plants overwintered in the field, or on flower branches and seed-bearing stalks that developed during the growing season. Lesions girdled a branch or stalk, and killed or weakened it so that it broke during pod fill. Isolates of Botrytis cinerea Pers.:Fr. were obtained by plating spores from lesions onto potato dextrose agar. To confirm pathogenicity, stems of 12-day-old seedlings of ‘Wk 121’ were scraped with a razor blade or left intact, atomized with sterile 0.01 % Tween 80 or a suspension of Botrytis cinerea at 1.0 × 106 conidia/ml, and kept at 20°C in a dew chamber in plastic bags. The fungus was reisolated from small lesions on wounded stems inoculated with B. cinerea after 3 days. No lesions developed on non-wounded or wounded control plants. B. cinerea is reported to cause storage rot of cabbage (2) and gray mold on Brassica oleracea L. (cabbage, kale, kohlrabi, wild cabbage) in Washington (1) but not stem canker. This new seed crop disease may be the result of predisposition to infection by freezing injury or mechanical damage on a highly susceptible cultivar grown under cool, wet weather. References: (1) D. F. Farr et al. 1989. Fungi on Plants and Plant Products in the United States. American Phytopathological Society. St. Paul, MN. (2) O. C. Yoder and M. L. Whalen. Can. J. Bot. 53:691, 1975.

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Suicidal attempt by woman in labor

International Journal of Reproduction Contraception Obstetrics and Gynecology ◽

10.18203/2320-1770.ijrcog20175302 ◽

2017 ◽

Vol 6 (12) ◽

pp. 5650

Author(s):

Vidyadhar B. Bangal ◽

Krishna Mandade ◽

Priyanka Verma ◽

Prachi Amrale ◽

Kunal Aher ◽

...

Keyword(s):

Suicidal Ideation ◽

Associated Factors ◽

Attempted Suicide ◽

Razor Blade ◽

Fetal Demise ◽

Contributory Factors ◽

Active Labour ◽

Suicidal Attempts ◽

Unwanted Pregnancies ◽

Labour Room

Suicidal ideation during delivery is extremely rare. Very few cases have been reported in the literature. Unwanted pregnancies, pre-existent mental disorders, substance abuse or addictions, marital dis-harmony, fetal demise or stillbirths have been documented as associated factors in reported cases of suicidal attempts by women in labour. Women tried taking overdose of sedative pills or have attempted hanging, drowning or jumping from hospital terrace. Lack of prenatal education, suboptimum use of labour analgesia, ill-treatment by health professional and relatives have been the contributory factors for suicidal ideation during pregnancy and labour. We report a rare case of attempted suicide by woman in active labour. She tried to cut the forearm and wrists with the help of razor blade used for preparation of part in preparation room attached to the labour room. The situation was recognized early by vigilant nurse on duty. The woman was resuscitated, treated, and counselled by psychiatrist before discharge from the hospital.

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Embedding Cultured Cells Grown in Well Plates

Microscopy Today ◽

10.1017/s1551929500057680 ◽

2006 ◽

Vol 14 (3) ◽

pp. 50-52

Author(s):

Leona Cohen-Gould

Keyword(s):

Electron Microscopy ◽

Cross Sections ◽

Cultured Cells ◽

List Type ◽

Type Number ◽

Razor Blade ◽

Cell Monolayers ◽

Block Face ◽

Resin Mixture

For years, I have used a hybrid Epon-analog resin to embed in culture dishes. I use a standard Epon formula but utilize the following components: LX-112 and DMP-30 from Ladd Research Industries DDSA and NMA from Electron Microscopy Sciences I know it seems weird, but years ago I tried all sorts things, from the “straight” formulations from each vendor to a bunch of mixtures. This one has never reacted with the plastic of the dishes. Here is how I do the actual embedding of the cell monolayers in the dishes:1)After the last 100% ethanol, remove the alcohol and cover the bottom of the well with a layer of resin mixture that is about 2 mm deep.2)Insert embedding tubes that are made by cutting the pyramidal bottoms off of BEEM capsules (just slice them with a fresh razor blade and be sure to insert them so that the manufactured end rather than the cut one is sitting against the dish).3)After inserting labels into the tubes, put them into the oven at 60° overnight.4)In the morning, fill just the embedding tubes and return everything to the oven again to finish polymerizing.5)When the resin is cured, grab the tubes with a pair of needlenosed pliers and snap them out. Sometimes a bit of the bottom of the dish comes away with the block, but often a very smooth block face results. If some of the dish comes up, it is easy to see under a dissecting microscope, and the dish portion comes away easily when trimming the block face.I often cut away part of the block face with a jeweler's saw, either to keep it in reserve or to re-embed it in order to get cross sections, and then trim the rest into a narrow rectangle.

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