Effect of Methanol Extract of Polygonum minus on Neuropathic Pain and Cognitive Dysfunction in Rats

This study evaluated the biological activity (antioxidant assay) of Polygonum minus extracted using Supercritical Fluid Extraction (SFE) added with different types of co-solvents. The seven co-solvents employed were water, methanol, ethanol, 50% methanol, 50% ethanol, 70% methanol and 70% ethanol for selection of the best co-solvent prior to optimization of SFE. 70% methanol produced the highest total yield of extract (33.1%) compared to other co-solvents. The antioxidant capacity was then evaluated using four different assays: the total phenolic content (TP), the total flavonoid content (TF), the ferric reducing/antioxidant power (FRAP) and the free radical-scavenging capacity of 2,2-diphenyl-1-picrylhydrazyl (DPPH). The highest TP and TF were from 70% methanol extract (11.2 ± 0.15 mg GAE/g sample (mg GAE/g) and 11.9 ± 0.03 mg CAE/g sample (mg CEQ/g) respectively). 70% metanol extract also showed the highest FRAP value (346.7 ± 0.66 µmol Fe (II)/g sample) and the highest percentage of DPPH radical inhibition was also shown by 70% methanol extract (88.7 ± 0.40%). There was a positive correlation between the antioxidant capacity (FRAP and DPPH) with those of TP and TF contents. Therefore, the best co-solvent chosen for further optimization of SFE is 70% methanol.

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Anti-hypernociceptive effects of methanol extract of Boswellia dalzielii on STZ-induced diabetic neuropathic pain

Advances in Traditional Medicine ◽

10.1007/s13596-019-00411-y ◽

2020 ◽

Vol 20 (3) ◽

pp. 405-417

Author(s):

Marius Mbiantcha ◽

Rauf Khalid ◽

Donatien Albert Atsamo ◽

Isaac S. Njoku ◽

Arif Mehreen ◽

...

Keyword(s):

Neuropathic Pain ◽

Methanol Extract ◽

Diabetic Neuropathic Pain

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Antioxidant Activity Of Methanol Extract Of Kesum Leaves (Polygonum Minus Huds.) DPPH MethodAktivitas Antioksidan Ekstrak Metanol Daun Kesum (Polygonum Minus Huds.) Metode DPPH

Jurnal Laboratorium Khatulistiwa ◽

10.30602/jlk.v1i2.156 ◽

2018 ◽

Vol 1 (2) ◽

pp. 161 ◽

Cited By ~ 1

Author(s):

Indah Purwaningsih ◽

Reskiya Sapriani ◽

Ratih Indrawati

Keyword(s):

Antioxidant Activity ◽

Vitamin C ◽

Methanol Extract ◽

Beta Carotene ◽

Polar Compounds ◽

Antioxidant Compounds ◽

Dpph Method ◽

Polygonum Minus ◽

The Difference ◽

X 24

Abstract: Kesum Plants (Polygonum minus) is one of the endemic plants in West Borneo containing phenolic compounds, steroids, flavonoids, alkaloids, tannins, saponins, and beta-carotene. Phenolic, flavonoids and beta-carotene are antioxidant compounds. Most phenolic and flavonoid groups are polar compounds. Therefore, in this research used methanol solvent as representative of polar solvent. The purpose of this research was to know the difference of antioxidant activity of methanol extract of kesum leaves with vitamin C. The extraction is done by maseration for 3 x 24 hours, where the solvent is changed every 24 hours. The method used to measure antioxidant activity is the DPPH method (2,2-diphenyl-1-picrylhydrazyl). The samples used in this paper are methanol extract of kesum leaves in 80, 65, 50, 35, and 20 ppm with five replications. The comparator used is vitamin C. The result of the research showed that IC50 of methanol extract was 20,632 ppm and IC50 of vitamin C 6,175 ppm. Based on the results of this study it can be concluded that vitamin C and methanol extract of serum leaves have a very strong antioxidant activity.Abstrak: Tanaman Kesum (Polygonum minus) merupakan salah satu tanaman endemik di wilayah Kalimantan Barat yang mengandung senyawa golongan fenolik, steroid, flavonoid, alkaloid, tanin, saponin, dan betakaroten. Golongan fenolik, flavonoid dan betakaroten merupakan senyawa antioksidan. Sebagian besar golongan fenolik dan flavonoid merupakan senyawa polar. Maka dari itu, pada penelitian ini digunakan pelarut metanol sebagai perwakilan pelarut polar. Tujuan penelitian ini untuk mengetahui perbedaan aktivitas antioksidan ekstrak metanol daun kesum terhadap vitamin C. Proses ekstraksi dilakukan dengan cara maserasi selama 3 x 24 jam, dimana pelarutnya diganti setiap 24 jam sekali. Metode yang digunakan untuk mengukur aktivitas antioksidan adalah metode DPPH (2,2-difenil-1-pikrilhidrazil). Sampel yang digunakan pada penelitian ini yaitu ekstrak metanol daun kesum konsentrasi 80, 65, 50, 35, dan 20 ppm dengan pengulangan sebanyak lima kali. Pembanding yang digunakan adalah vitamin C. Hasil penelitian didapatkan IC50 ekstrak metanol daun kesum sebesar 20.632 ppm dan IC50 vitamin C sebesar 6.175 ppm. Berdasarkan hasil penelitian dapat disimpulkan bahwa vitamin C dan ekstrak metanol daun kesum memiliki aktivitas antioksidan yang sangat kuat.

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Cytotoxicity and antiviral activity of methanol extract from Polygonum minus

10.1063/1.4931257 ◽

2015 ◽

Author(s):

Noor Zarina Abd Wahab ◽

Hamidun Bunawan ◽

Nazlina Ibrahim

Keyword(s):

Antiviral Activity ◽

Methanol Extract ◽

Polygonum Minus

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Treatment with anti-interleukin-6 receptor antibody improves mechanical hypersensitivity and cognitive dysfunction in mice with neuropathic pain

Proceedings for Annual Meeting of The Japanese Pharmacological Society ◽

10.1254/jpssuppl.94.0_2-y-f2-2 ◽

2021 ◽

Vol 94 (0) ◽

pp. 2-Y-F2-2

Author(s):

Kodai Moriwaki ◽

Kazue Nakashima ◽

Toshiki Yoshii ◽

Yoki Nakamura ◽

Norimitsu Morioka

Keyword(s):

Neuropathic Pain ◽

Cognitive Dysfunction ◽

Interleukin 6 ◽

Mechanical Hypersensitivity ◽

Receptor Antibody ◽

Interleukin 6 Receptor

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Gut Microbiome and Plasma Metabolome Signatures in Middle-Aged Mice With Cognitive Dysfunction Induced by Chronic Neuropathic Pain

Frontiers in Molecular Neuroscience ◽

10.3389/fnmol.2021.806700 ◽

2022 ◽

Vol 14 ◽

Author(s):

Dongyu Hua ◽

Shan Li ◽

Shiyong Li ◽

Xuan Wang ◽

Yue Wang ◽

...

Keyword(s):

Neuropathic Pain ◽

Gut Microbiota ◽

Cognitive Dysfunction ◽

Gut Bacteria ◽

Morris Water Maze Test ◽

Middle Aged ◽

Chronic Neuropathic Pain ◽

Fecal Microbiome ◽

Speed Of Information Processing ◽

Plasma Metabolome

Patients with chronic neuropathic pain (CNP) often complain about their terrible memory, especially the speed of information processing. Accumulating evidence suggests a possible link between gut microbiota and pain processing as well as cognitive function via the microbiota-gut-brain axis. This study aimed at exploring the fecal microbiome and plasma metabolite profiles in middle-aged spared nerve injury (SNI) mice model with cognitive dysfunction (CD) induced by CNP. The hierarchical cluster analysis of performance in the Morris water maze test was used to classify SNI mice with CD or without CD [i.e., non-CD (NCD)] phenotype. 16S rRNA sequencing revealed a lower diversity of gut bacteria in SNI mice, and the increase of Actinobacteria, Proteus, and Bifidobacterium might contribute to the cognitive impairment in the CNP condition. The plasma metabolome analysis showed that the endocannabinoid (eCB) system, disturbances of lipids, and amino acid metabolism might be the dominant signatures of CD mice. The fecal microbiota transplantation of the Sham (not CD) group improved allodynia and cognitive performance in pseudo-germ-free mice via normalizing the mRNA expression of eCB receptors, such as cn1r, cn2r, and htr1a, reflecting the effects of gut bacteria on metabolic activity. Collectively, the findings of this study suggest that the modulation of gut microbiota and eCB signaling may serve as therapeutic targets for cognitive deficits in patients with CNP.

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