Antioxidant Activity Of Methanol Extract Of Kesum Leaves (Polygonum Minus Huds.) DPPH MethodAktivitas Antioksidan Ekstrak Metanol Daun Kesum (Polygonum Minus Huds.) Metode DPPH

2018 ◽  
Vol 1 (2) ◽  
pp. 161 ◽  
Author(s):  
Indah Purwaningsih ◽  
Reskiya Sapriani ◽  
Ratih Indrawati
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Methanol Extract ◽  
Beta Carotene ◽  
Polar Compounds ◽  
Antioxidant Compounds ◽  
Dpph Method ◽  
Polygonum Minus ◽  
The Difference ◽  
X 24

Abstract: Kesum Plants (Polygonum minus) is one of the endemic plants in West Borneo containing phenolic compounds, steroids, flavonoids, alkaloids, tannins, saponins, and beta-carotene. Phenolic, flavonoids and beta-carotene are antioxidant compounds. Most phenolic and flavonoid groups are polar compounds. Therefore, in this research used methanol solvent as representative of polar solvent. The purpose of this research  was to know the difference of antioxidant activity of methanol extract of kesum leaves with vitamin C. The extraction  is done by maseration for 3 x 24 hours, where the solvent is changed every 24 hours. The method used to measure antioxidant activity is the DPPH method (2,2-diphenyl-1-picrylhydrazyl). The samples used in this paper are methanol extract of  kesum leaves in 80, 65, 50, 35, and 20 ppm with five replications. The comparator used is vitamin C. The result of the research showed that IC50 of methanol extract was 20,632 ppm and IC50 of vitamin C 6,175 ppm. Based on the results of this study it can be concluded that vitamin C and methanol extract of serum leaves have a very strong antioxidant activity.Abstrak: Tanaman Kesum (Polygonum minus) merupakan salah satu tanaman endemik di wilayah Kalimantan Barat yang mengandung senyawa golongan fenolik, steroid, flavonoid, alkaloid, tanin, saponin, dan betakaroten. Golongan fenolik, flavonoid dan betakaroten merupakan senyawa antioksidan. Sebagian besar golongan fenolik dan flavonoid merupakan senyawa polar. Maka dari itu, pada penelitian ini digunakan pelarut metanol sebagai perwakilan pelarut polar. Tujuan penelitian ini untuk mengetahui perbedaan aktivitas antioksidan ekstrak metanol daun kesum terhadap vitamin C. Proses ekstraksi dilakukan dengan cara maserasi selama 3 x 24 jam, dimana pelarutnya diganti setiap 24 jam sekali. Metode yang digunakan untuk mengukur aktivitas antioksidan adalah metode DPPH (2,2-difenil-1-pikrilhidrazil). Sampel yang digunakan pada penelitian ini yaitu ekstrak metanol daun kesum konsentrasi 80, 65, 50, 35, dan 20 ppm dengan pengulangan sebanyak lima kali. Pembanding yang digunakan adalah vitamin C. Hasil penelitian didapatkan IC50 ekstrak metanol daun kesum sebesar 20.632 ppm dan IC50 vitamin C sebesar 6.175 ppm. Berdasarkan hasil penelitian dapat disimpulkan bahwa vitamin C dan ekstrak metanol daun kesum memiliki aktivitas antioksidan yang sangat kuat.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL BULBUS BAWANG DAYAK (Eleutherine americana Merr) DENGAN METODE DPPH (1,1-Diphenyl-2-Picrylhydrazyl)

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 451
Author(s):  
Rizkah V. Mokoginta ◽  
Herny E. I. Simbala ◽  
Karlah L.R Mansauda
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethanol Extract ◽  
Antioxidant Compounds ◽  
Onion Plant ◽  
Dpph Method ◽  
Ic50 Values ◽  
Experimental Laboratory ◽  
Ethanol Extracts ◽  
Eleutherine Americana

ABSTRACTDayak onion plant (Eleutherine americana Merr.) or commonly called forest onion is a typical plant of Central Kalimantan that has been used by local peoples for cure a various types of diseases. This study aims to determine the activity of antioxidant compounds from Dayak bulb ethanol extracts from Kotamobagu City. This research method is an experimental laboratory. Initially the Dayak bulb sample was extracted maceration with 96% ethanol solvent then evaporated until a thick extract was obtained. As a parameter, the DPPH method (1,1-diphenyl-2-picrylhydrazyl) was used to test the antioxidant activity of the samples at concentrations of 50, 60, 70, 80 and 90 mg / L and as a comparison standard used Vitamin C with concentrations of 2, 4, 6, 8 and 10 mg / L. The results obtained showed antioxidant activity in Dayak bulb ethanol extracts with IC50 values of 41.46 mg / L and on vitamin C IC50 values of 1.04 mg / L. Based on these results it can be concluded that the onion bulb ethanol extract has very strong antioxidant activity. Keywords: Dayak Onion bulbs, Activity, Antioxidant, DPPH  ABSTRAK Tanaman bawang Dayak (Eleutherine americana Merr.) atau biasa disebut bawang hutan merupakan tanaman khas Kalimantan Tengah yang sudah dipergunakan masyarakat lokal sebagai obat berbagai jenis penyakit. Penelitian ini bertujuan untuk mengetahui aktivitas senyawa antioksidan dari ekstrak etanol bulbus bawang Dayak yang berasal dari Kota Kotamobagu. Metode penelitian ini adalah eksperimental laboratorium. Awalnya sampel bulbus bawang Dayak diekstraksi maserasi dengan pelarut etanol 96% kemudian dievaporasi hingga didapatkan ekstrak kental. Sebagai parameter, digunakan metode DPPH (1,1-diphenyl-2-picrylhydrazyl) untuk pengujian aktivitas antioksidan sampel pada konsentrasi 50, 60, 70, 80 dan 90 mg/L dan sebagai standar pembanding digunakan Vitamin C dengan konsentrasi 2, 4, 6, 8 dan 10 mg/L. Hasil penelitian yang didapat menunjukkan aktivitas antioksidan pada ekstrak etanol bulbus bawang Dayak dengan nilai IC50 sebesar 41,46 mg/L dan pada vitamin C nilai IC50 sebesar 1,04 mg/L. Berdasarkan hasil tersebut dapat disimpulkan bahwa ekstrak etanol bulbus bawang Dayak memiliki aktivitas antioksidan yang sangat kuat. Kata kunci: Bulbus Bawang Dayak, Aktivitas, Antioksidan, DPPH

scholarly journals ISOLATION AND CHARACTERIZATION OF ANTIOXIDANT COMPOUNDS FROM ETHYL ACETATE AND METHANOL EXTRACTS OF GARCINIA DAEDALANTHERA PIERRE LEAVES

2018 ◽  
Vol 10 (1) ◽  
pp. 276
Author(s):  
Anisa Puspitaningrum ◽  
Berna Elya ◽  
Katrin .
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Column Chromatography ◽  
Spectrum Analysis ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Uv Light ◽  
Antioxidant Compounds ◽  
Isolation And Characterization ◽  
Dpph Method

Objective: This study aimed to isolate and characterize the compounds responsible for the high antioxidant activities of the ethyl acetate and methanolextracts of Garcinia daedalanthera Pierre leaves.Methods: In this study, the ethyl acetate extract was obtained by column chromatography, and the methanol extract was obtained by vacuum columnchromatography. The mobile phase comprised n-hexane, ethyl acetate, and methanol with increased polarity. Antioxidant activity was examined usingthe 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The fraction with the highest antioxidant activity was purified through column chromatography,recrystallization, and preparative thin-layer chromatography. This fraction, termed the isolate of B, was identified using DPPH and AlCl3, and itsantioxidant activity was quantitatively tested.Results: From this research, 21.7 mg of the isolate of B were obtained with an IC50 of 5.82 μg/mL. Identification using an AlCl3 sprayer producedyellow phosphorescent spots under UV light. UV-Vis spectrum analysis revealed the presence of an aromatic compound and conjugated double bonds.Infrared spectrum analysis revealed the presence of −OH, C–H alkane, C=C aromatic, C=O, and C-O-C groups.Conclusion: Based on this research, 21.7 mg of the isolate of B was derived through fractionation of the methanol extract, and this isolate exhibitedantioxidant activity with an IC50 of 5.82 μg/mL. The isolate of B was considered to be a flavonoid, as it was fluorescent under UV light (366 nm) afterbeing sprayed with AlCl3 reagent.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL KULIT BATANG MAHONI (Swietenia mahagoni Jacq.) MENGGUNAKAN METODE DPPH (1,1 diphenyl-2-picrylhydrazyl)

PHARMACON ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 548
Author(s):  
Amanda T. M. Turangan ◽  
Defny S. Wewengkang ◽  
Adithya Yudistira
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Vitamin C ◽  
Medicinal Plant ◽  
Positive Control ◽  
Antioxidant Compounds ◽  
Phytochemical Screening ◽  
Oxidation Reactions ◽  
Dpph Method ◽  
Swietenia Mahagoni

Antioxidant are compounds that are able to inactivate the development of the oxidation reactions of the molecules or neutralize free radicals. Mahogany bark (Swietenia mahagoni Jacq.) has properties as a medicinal plant. Through the phytochemical screening, the mahogany bark (Swietenia mahagoni Jacq.) contains antioxidant compounds such as alkaloids, saponins and phenolics. The aim of this study was to determine the presence of antioxsidant activity in the extract of mahogany bark (Swietenia mahagoni Jacq.). using the DPPH method with a concentration of 250 mg / L, 200 mg / L, 150 mg / L and 100 mg / L and Vitamin C p.a as a positive control. Each sample made three repetitions of the test. The test uses a UV-Vis Spectrophotometer. The result of the study showed that mahogany bark (Swietenia mahagoni Jacq.) showed antioxidant activity with the percentage of 21,50% at the concentration at 250 mg / LKeywords : Swietenia mahagoni Jacq., Antioxidant, Ethanol, DPPHABSTRAKAntioksidan ialah senyawa yang mampu menginaktivasi berkembangnya reaksi oksidasi molekul lain atau menetralisir radikal bebas. Kulit Batang Mahoni (Swietenia mahagoni Jacq.) memiliki khasiat sebagai tanaman obat. Melalui skrining fitokimia, Swietenia mahagoni Jacq. Mengandung senyawa antioksidan seperti alkaloid, saponin dan fenolik. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antioksidan didalam ekstrak tanaman Kulit Batang Switenia mahagoni Jacq. menggunakan metode DPPH dengan konsentrasi 250 mg/L, 200 mg/L, 150 mg/L dan 100 mg/L dan Vitamin C p.a sebagai kontrol positif. Masing-masing sampel dibuat tiga kali pengulangan uji. Pengujian menggunakan alat Spektrofotometer UV-Vis. Hasil penelitian menunjukkan bahwa ekstrak Kulit Batang Swietenia mahagoni Jacq. memiliki aktivitas antioksidan dengan presentase sebesar 21,50% pada konsentrasi 250 mg/L Kata Kunci : Swietenia mahagoni Jacq., Antioksidan, Etanol, DPPH

Identification and Quantification of Antioxidant Compounds in Clarified Cashew Apple Juice ‘Cajuína’

2020 ◽  
Vol 16 (4) ◽  
pp. 585-591
Author(s):  
Rayssa G.L. Porto-Luz ◽  
Amanda J.B. de Moura ◽  
Bibiana da Silva ◽  
Roseane Fett ◽  
Marcos A. da Mota Araújo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Apple Juice ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Flavonoid Content ◽  
Cashew Apple Juice ◽  
Dpph Method ◽  
Significant Difference ◽  
Cashew Apple

Background: Cajuína is obtained through clarification of cashew apple juice (Anacardium occidentale L.), a non-diluted and non-fermented beverage, which is prepared from the edible part of the pseudo-fruit. Objective: This study sought to identify and quantify phenolic compounds, determine vitamin C content, total flavonoid content, and the antioxidant activity of two commercial brands of clarified cashew apple juice (cajuína) produced in Piauí, Brazil. Methods: Spectrophotometric and high performance liquid chromatography were used. Results: A high vitamin C content was found mainly in brand A. A high total phenolic content was observed in brand A, and there was no significant difference in flavonoid content between the two brands. Cajuína had high antioxidant activity, which was confirmed by two methods. The highest value was found for brand A by the DPPH method. Conclusion: We concluded that this clarified cashew apple juice has a high antioxidant compound content, and it is a functional food.

scholarly journals ANTIOXIDANT ACTIVITY OF LEAF EXTRACT FROM SIDA SUBCORDATA SPAN

2017 ◽  
Vol 1 (2) ◽  
Author(s):  
Rahma Yulia ◽  
Dachriyanus Dachriyanus ◽  
Amri Bachtiar
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Compounds ◽  
Spectrophotometric Methods ◽  
Dpph Method ◽  
Extract Fraction

This Study has tested the antioxidant activity of the extracts from Sida subcordata Span. Testing is done with antioxidant activity using DPPH method provides. Testing of antioxidant activity in vitro with spectrophotometric methods based on absorbance measurements radical DPPH (1,1-diphenylpicrylhydrazyl). All the extracts Showed radical scavenging activity. The ethyl acetate and methanol extracts were the most Appropriate as sources of antioxidant compounds as shown by Reviews their inhibition percentage (IP) values. Percentage inhibitor to extract ethyl acetate and methanol extract respectively 76.16% and 79.28% at a concentration of 1 mg / ml to 0.05 mM DPPH. while the results of the testing activities of antioxidant compounds y pure ethyl acetate extract fraction showed a smaller percentage inhibitor that is equal to 30% at the same concentration. Keyword - Sida subcordata Span, Malvaceae, Antioxidant, Alkaloid

scholarly journals Antioxidant Activity of Methanol Extract of the Purun Tikus (Eleocharis dulcis) Leaves

2018 ◽  
Vol 3 (3) ◽  
pp. 135 ◽  
Author(s):  
Kholifatu Rosyidah ◽  
Taufiqur Rohman ◽  
Ridhana Fitriani
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Vitamin C ◽  
Methanol Extract ◽  
Open Water ◽  
Extreme Conditions ◽  
Phytochemical Screening ◽  
Strong Activity ◽  
Dpph Method ◽  
Acidic Sulfate

<p>Purun tikus (<em>Eleocharis dulcis</em>) is a kind of swamp plant, can be found in open water areas, both in swamps and rice fields. <em>Purun tikus</em> is an indicator vegetation of acidic sulfate soil. Chemically, plants that able to adapt to special or extreme conditions, usually produce a compound that are unique and have unique bioactivity as well. This paper reports a phytochemical screening of <em>Purun tikus</em> methanol extract and its antioxidant activity. The results showed that the methanol extract of <em>Purun tikus</em> leaves contained phenolic compounds, flavonoids, tannins and terpenoids. By using DPPH method as an antioxidant test, the methanol extract of purun tikus leaves showed a strong activity, with IC<sub>50</sub> value was 58.58 ppm while vitamin C had an IC<sub>50</sub> value of 4.50 ppm.</p>

scholarly journals Aktivitas Antioksidan Ekstrak Metanol Biji Langsat (Lansium domesticum var. pubescens) dan Duku (Lansium domesticum var. domesticum) dengan Metode DPPH

2020 ◽  
Vol 20 (2) ◽  
pp. 113
Author(s):  
Josepin P Konda ◽  
Jainer P Siampa ◽  
Trina E Tallei ◽  
Billy J Kepel ◽  
Fatimawali Fatimawali
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Methanol Extract ◽  
Regression Line ◽  
Seed Extract ◽  
Test Method ◽  
Color Test ◽  
Dpph Method ◽  
Lansium Domesticum ◽  
Positive Results

Penelitian ini bertujuan untuk mengetahui kandungan fitokimia dan aktivitas antioksidan ekstrak biji langsat dan duku dengan metode DPPH. Biji buah langsat dan buah duku diekstraksi menggunakan metode maserasi dengan pelarut metanol. Skrining fitokimia yang dilakukan meliputi uji alkaloid, flavonoid, tannin, triterpenoid, steroid dan saponin secara kualitatif dengan metode uji warna. Uji aktivitas antioksidan menggunakan metode DPPH dan vitamin C sebagai pembanding, serapan diukur menggunakan spektrofotometer UV-Vis pada panjang gelombang 517 nm. Skrining fitokimia pada ekstrak metanol biji langsat menunjukkan hasil positif terhadap alkaloid, triterpenoid dan saponin, sementara pada ekstrak metanol biji duku menunjukkan hasil positif terhadap alkaloid, flavonoid, tannin, triterpenoid, steroid dan saponin. Aktivitas antioksidan ekstrak biji langsat dianalisis menggunakan persamaan garis regresi y = 0,2922x + 33,143, diperoleh IC₅₀ 57,69 μg/mL. Sedangkan aktivitas antioksidan ekstrak biji duku dianalisis menggunakan persamaan garis regresi y = 0.6554x + 29.421, diperoleh IC₅₀ 31,40 μg/mL dan pembanding vitamin C diperoleh IC₅₀ 8,03 μg/mL. Hasil penelitian ini dapat disimpulkan bahwa ekstrak metanol biji buah langsat memiliki kandungan alkaloid, triterpenoid dan saponin serta memiliki aktivitas antioksidan yang kuat sedangkan ekstrak metanol biji buah duku memiliki kandungan alkaloid, flavonoid, tannin, triterpenoid dan saponin serta memiliki aktivitas antioksidan yang sangat kuat.Kata Kunci: Antioksidan, biji duku, biji langsat Antioxidant Activity of Methanol Extract of Langsat Seeds (Lansium domesticum var. pubescens) and Duku Seeds (Lansium domesticum var. domesticum)  by Using DPPH Method ABSTRACTThis study aimed to determine the phytochemical content and antioxidant activity of langsat seed extract and duku seed extract by using the DPPH method. Langsat seeds and duku seeds were extracted by using maceration method with methanol as the solvent. Phytochemical screening was carried out including a qualitative test of alkaloids, flavonoids, steroids/triterpenoids, saponins, and tannins by using the color test method. Antioxidant activity was tested by using the DPPH method and vitamin C used as comparison, the absorption was measured by using UV-Vis spectrophotometer at 517 nm wavelength. The result of phytocemical screening on methanol extract of langsat seeds showed positive results on alkaloids, triterpenoids, and saponins, meanwhile methanol extract of duku seeds showed positive results on alkaloids, flavonoids, triterpenoids, tannin and saponins. The antioxidant activity of methanol extract of langsat seeds was analyzed by using the equation of the regression line y = 0.2922x + 33.143, which obtained IC₅₀ value of 57.69 μg/mL. Meanwhile, the antioxidant activity of methanol extract of duku seeds was analyzed by using the equation of the regression line y = 0.6554x + 29.421, obtained IC₅₀ 31,40 μg/mL. Vitamin C obtained IC₅₀ 8.03 μg/mL. On the basis of all results, it can be concluded that the methanol extract of langsat seeds contained alkaloids, saponins and triterpenoid and has a strong antioxidant activity. Meanwhile, the methanol extract of duku seeds contained alkaloids, flavonoids, triterpenoids, tannin and saponins and has a very strong antioxidant activity.Keywords: Antioxidants, duku seeds, langsat seeds

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

2019 ◽  
Vol 5 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Asriullah Jabbar ◽  
Wahyuni Wahyuni ◽  
Muh Hajrul Malaka ◽  
Apriliani Apriliani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Typhoid Fever ◽  
Skin Diseases ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN DARI EKSTRAK ETANOL SPONS Ianthella basta DARI DESA TUMBAK KECAMATAN PUSOMAEN KABUPATEN MINAHASA TENGGARA

PHARMACON ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 713
Author(s):  
Nurhati Anton ◽  
Adithya Yudistira ◽  
Jainer Pasca Siampa
Keyword(s):  
Antioxidant Activity ◽  
Coral Reef ◽  
Vitamin C ◽  
Bioactive Compounds ◽  
Ethanol Extract ◽  
Positive Control ◽  
Terrestrial Plants ◽  
Dpph Method ◽  
Ethanol Extracts

ABSTRACT Sponges are a component of coral reef biota that has bioactive compounds with a greater percentage of activity compared to compounds produced by terrestrial plants. This study aims to determine the presence of antioxidant activity in the ethanol extracts of sponge Ianthella basta from the waters of Tumbak Village. Ianthella basta sponge was extracted using maceration method with 96% ethanol solvent. Antioxidant activity testing was carried out using the DPPH method with a concentration of 100 µg / mL, 75 μg / mL, 50 μg / mL, and 25 μg / mL and Vitamin C p.a as positive control. Each sample was made three repetitions of the test. Testing using a UV-Vis spectrophotometer. The results showed that the ethanol extract of the Ianthella basta sponge had antioxidant activity with a percentage of 48.73% at a concentration of 100 µg / mL. Keywords: Ianthella basta sponge, Antioxidants, Extraction, DPPH  ABSTRAK Spons merupakan salah satu komponen biota penyusun terumbu karang yang mempunyai senyawa bioaktif dengan persentase keaktifan lebih besar dibandingkan dengan senyawa-senyawa yang dihasilkan oleh tumbuhan darat. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antioksidan di dalam ekstrak etanol Spons Ianthella basta dari perairan Desa Tumbak. Spons Ianthella basta diekstraksi menggunakan metode maserasi dengan pelarut etanol 96%. Pengujian aktivitas antioksidan dilakukan dengan menggunakan metode DPPH dengan konsentrasi 100 µg/mL, 75 µg/mL, 50 µg/mL, dan 25 µg/mL dan Vitamin C p.a sebagai kontrol positif. Masing-masing sampel dibuat tiga kali pengulangan uji. Pengujian menggunakan alat spektrofotometer UV-Vis. Hasil penelitian menunjukkan bahwa ektrak etanol Spons Ianthella basta memiliki aktivitas antioksidan dengan persentase sebesar 48,73% pada konsentrasi 100 µg/mL. Kata Kunci: Spons Ianthella basta, Antioksidan, Ekstraksi, DPPH

scholarly journals Limpasu Pericarpium : an Alternative Source of Antioxidant From Borneo with Sequential Maceration Method

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Irfan Zamzani ◽  
Nita Triadisti
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Oxidation Process ◽  
Antioxidant Activities ◽  
Alternative Source ◽  
Dpph Method ◽  
Indigenous Plant ◽  
Ic50 Values

Antioxidants are substances that can slow down the oxidation process of free radicals. Limpasu plant (Baccaurea lanceolata (Miq) Muell. Arg), an indigenous plant of Borneo, is a natural antioxidant source. The purpose of this study was to determine the antioxidant activity of the limpasu pericarpium extract. The extraction of the limpasu pericarpium was done by maceration method using solvents with increasing polarity ranging from n-hexane, ethyl acetate, and methanol. Antioxidant activities of the three extracts were measured by the DPPH and FRAP methods. The IC50 values of n-hexane, ethyl acetate, and methanol extracts, as well as quercetin using the DPPH method were 517,45 µg/mL, 530,64 µg/mL, 10,63 µg/mL and 6,83 µg/mL, respectively. Meanwhile, the IC50 values obtained from FRAP method were 198,96 µg/mL, 190,07 µg/mL, 661,36 µg/mL, and 7,09 µg/mL, respectively. The results revealed that the methanol extract is more potent than other extracts tested for antioxidant activity.

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