scholarly journals Utilizing flexible bronchoscopy for the diagnosis of endobronchial tuberculosis with negative sputum acid-fast bacillus

2021 ◽  
Vol 9 (1) ◽  
pp. 1
Author(s):  
Nai-Chien Huan ◽  
Nurul IzzatiAzmin Mohd Yusof ◽  
HemaYamini Ramarmuty ◽  
TengShin Khoo ◽  
YeanChen Lai ◽  
...  
Keyword(s):  
Acid Fast Bacillus ◽  
Flexible Bronchoscopy ◽  
Endobronchial Tuberculosis ◽  
Negative Sputum

scholarly journals Use of flexible bronchoscopy for rapid diagnosis of suspected tubercular cases in rural India

2009 ◽  
Vol 3 (11) ◽  
pp. 860-864 ◽  
Author(s):  
Sameer Singhal ◽  
Abhay M. Gaidhane ◽  
Nazli Khatib ◽  
Tripti Hrivastava ◽  
Sanjay Diwan ◽  
...  
Keyword(s):  
Diagnostic Yield ◽  
Correct Diagnosis ◽  
Positive Culture ◽  
Cross Sectional Study ◽  
Flexible Bronchoscopy ◽  
Special Importance ◽  
Cross Sectional ◽  
Tuberculosis Patients ◽  
Negative Sputum ◽  
Tuberculosis Therapy

Background: Reaching a correct diagnosis is a challenge for physicians treating any of the 30% to 50% of pulmonary tuberculosis patients who have negative sputum cultures or who present with no sputum.  Flexible bronchoscopy acquires special importance for these cases for whom empirical anti-tuberculosis therapy is the only option left. In our study we aimed to assess the diagnostic yield of flexible bronchoscopy in patients, suspected to have tuberculosis, whose sputum smears were negative. Methodology: In our hospital-based cross-sectional study, 42 patients were enrolled by consecutive sampling. Flexible bronchoscopy and selective bronchial washings were done in all patients.  Results: Bronchoscopy lavage smears were positive for M. tuberculosis in 10 (23.8%) patients. Fifteen (35.7%) patients had positive culture. Conclusion: Flexible bronchoscopy has an important role in the diagnosis of patients suspected to have tuberculosis, whose sputum smears are negative or who can not produce sputum.

scholarly journals A Novel Reporter Phage To Detect Tuberculosis and Rifampin Resistance in a High-HIV-Burden Population

2015 ◽  
Vol 53 (7) ◽  
pp. 2188-2194 ◽  
Author(s):  
Max R. O'Donnell ◽  
Alexander Pym ◽  
Paras Jain ◽  
Vanisha Munsamy ◽  
Allison Wolf ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Drug Susceptibility ◽  
Acid Fast Bacillus ◽  
Drug Susceptibility Testing ◽  
Response To Treatment ◽  
Fluorescent Reporter ◽  
Content Type ◽  
Rapid Enumeration ◽  
Rifampin Resistance ◽  
Negative Sputum

Improved diagnostics and drug susceptibility testing forMycobacterium tuberculosisare urgently needed. We developed a more powerful mycobacteriophage (Φ2GFP10) with a fluorescent reporter. Fluorescence-activated cell sorting (FACS) allows for rapid enumeration of metabolically active bacilli after phage infection. We compared the reporter phage assay to GeneXpert MTB/RIF for detection ofM. tuberculosisand rifampin (RIF) resistance in sputum. Patients suspected to have tuberculosis were prospectively enrolled in Durban, South Africa. Sputum was incubated with Φ2GFP10, in the presence and absence of RIF, and bacilli were enumerated using FACS. Sensitivity and specificity were compared to those of GeneXpert MTB/RIF with anM. tuberculosisculture as the reference standard. A total of 158 patients were prospectively enrolled. Overall sensitivity forM. tuberculosiswas 95.90% (95% confidence interval (CI), 90.69% to 98.64%), and specificity was 83.33% (95% CI, 67.18% to 93.59%). In acid-fast bacillus (AFB)-negative sputum, sensitivity was 88.89% (95% CI, 73.92% to 96.82%), and specificity was 83.33% (95% CI, 67.18% to 93.59%). Sensitivity for RIF-resistantM. tuberculosisin AFB-negative sputum was 90.00% (95% CI, 55.46% to 98.34%), and specificity was 91.94% (95% CI, 82.16% to 97.30%). Compared to GeneXpert, the reporter phage was more sensitive in AFB smear-negative sputum, but specificity was lower. The Φ2GFP10 reporter phage showed high sensitivity for detection ofM. tuberculosisand RIF resistance, including in AFB-negative sputum, and has the potential to improve phenotypic testing for complex drug resistance, paucibacillary sputum, response to treatment, and detection of mixed infection in clinical specimens.

Flexible Bronchoscopy for Diagnosis and Follow Up of Childhood Endobronchial Tuberculosis

2008 ◽  
Vol 27 (9) ◽  
pp. 783-787 ◽  
Author(s):  
Erkan Cakir ◽  
Zeynep Seda Uyan ◽  
Sedat Oktem ◽  
Fazilet Karakoc ◽  
Refika Ersu ◽  
...  
Keyword(s):  
Flexible Bronchoscopy ◽  
Endobronchial Tuberculosis

scholarly journals Endobronchial Tuberculosis: Two Case Reports and Review of the Literature

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Anshum Aneja ◽  
Uma Maheswari Krishnaswamy ◽  
Vijayashree Thyagaraj ◽  
Riyaz P. Moideen ◽  
Mantha Satya Padmaja
Keyword(s):  
Case Reports ◽  
False Negative ◽  
Delayed Diagnosis ◽  
Young Patients ◽  
Primary Diagnosis ◽  
Low Grade ◽  
Treatment And Prevention ◽  
Insidious Onset ◽  
Endobronchial Tuberculosis ◽  
Negative Sputum

Endobronchial tuberculosis commonly affects young patients and presents as acute or insidious onset cough, wheeze, low grade fever, and constitutional symptoms. Although endobronchial lesions usually result in sputum positivity for acid fast bacilli (AFB), a false negative sputum or absence of radiological lesions may result in delayed diagnosis. On the other hand, sputum positivity with presence of signs on chest radiology may lead to consideration of parenchymal TB as the primary diagnosis and the coexistence of endobronchial lesions may be missed until sequelae of the latter ensue. Besides, in elderly patients, consideration of other differentials like malignancy and pneumonia may lead to misdiagnosis. Hence, bronchoscopy is essential for confirmation of endobronchial TB. We hereby report two cases of endobronchial TB which stress the importance of bronchoscopic diagnosis for timely institution of treatment and prevention of permanent sequelae, respectively.

scholarly journals Value of bronchoscopy in the diagnosis of sputum smear negative pulmonary tuberculosis

2017 ◽  
Vol 5 (8) ◽  
pp. 3393
Author(s):  
Ilir Pajazit Peposhi ◽  
Perlat Qazim Kapisyzi ◽  
Hasan Sulejman Hafizi ◽  
Silvana Abdi Bala ◽  
Ornela Gani Nuredini
Keyword(s):  
Acid Fast Bacillus ◽  
Fibreoptic Bronchoscopy ◽  
Sputum Smear ◽  
Suspected Tuberculosis ◽  
Bronchial Washing ◽  
Endobronchial Tuberculosis ◽  
Smear Negative ◽  
Ulcerative Lesions ◽  
A Prospective Study ◽  
Acid Fast Bacillus Smear

Background: Bronchoscopy has been found to be applicable in diagnosing suspects of pulmonary TB which have no sputum or sputum smear negative for acid fast bacilli. Additionally, it is helpful in the diagnosis of endobronchial tuberculosis, allowing early detection of broncho stenosis.Methods: A prospective study, where bronchoscopy was conducted in 167 patients with acid-fast bacillus sputum smear negative. The average age of the patients was 44.1±19.4, from age 15 to 87 years old, higher frequency in age from 20-40 years old and 55-64 years old.Results: From 167 patients in the study, endoscopic presentation resulted: normal in 7 (4.2%) cases, bronchitis - 60 (35.9%) cases, oedematous-hyperaemic -62 (37.1%) cases, caseous inflammation - 7 (4.2%) cases, ulcerative lesions - 3 (1.8%) cases, tumoral lesions - 21 (12.6%) cases and fibrous stenotic changes in 7 (4.2%) patients. From 44 (26.3%) patients to whom was obtained biopsy, the histological examination has resulted: 24 (54.5%) cases with TB granuloma, 14 (31.8%) - epithelioid granulomas and in 6 (13.6%) cases resulted non-specific inflammation. The examination of bronchial washing for acid-fast bacillus smear concluded the diagnosis in 40.4% of cases, the examination for acid-fast bacillus smear collected after diagnostic FBS has been decisive in 53.9% of cases. The examination for acid-fast bacillus culture resulted positive in 70.5% of bronchial washing and in 61.2% of sputum collected after FBS.Conclusions: Fibreoptic bronchoscopy plays the key role to the patients with suspected tuberculosis which are acid-fast bacillus smear negative or that have no sputum.

Discontinuation of Respiratory Isolation for Possible Tuberculosis: Do Two Negative Sputum Smear Results Suffice?

2006 ◽  
Vol 27 (5) ◽  
pp. 515-516 ◽  
Author(s):  
Charles S. Bryan ◽  
Deborah J. Rapp ◽  
Cynthia A. Brown
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Analysis ◽  
Acid Fast Bacillus ◽  
Active Tuberculosis ◽  
Sputum Smear ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Negative Sputum ◽  
Acid Fast Bacillus Smear

Analysis of acid-fast bacillus smear results at a hospital with a mod-erate incidence of tuberculosis confirms recent recommendations that 2 negative smear results suffice for discontinuation of respiratory isolation. Use of polymerase chain reaction analysis further increases the confidence with which the diagnosis of active tuberculosis likely to be transmitted to others can be excluded.

Advances in Pediatric Flexible Bronchoscopy

1989 ◽  
Vol 22 (3) ◽  
pp. 545-551 ◽  
Author(s):  
James E. Arnold
Keyword(s):  
Flexible Bronchoscopy

Detection of Mycobacterium tuberculosis in smear negative sputum by PCR

2012 ◽  
Vol 6 (2) ◽  
pp. 2-6 ◽  
Author(s):  
Mohammad Jobayer ◽  
SM Shamsuzzaman ◽  
Kazi Zulfiquer Mamun
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Accurate Method ◽  
Major Health Problem ◽  
Major Health ◽  
Tuberculosis Patients ◽  
Diagnostic Potential ◽  
Total Death ◽  
Smear Negative ◽  
Negative Sputum

Pulmonary tuberculosis is a major health problem in Bangladesh that is responsible for about 7% of total death in a year. This study was conducted to isolate and identify Mycobacterium tuberculosis from sputum and to evaluate the efficacy of PCR as a modern diagnostic tool, for diagnosis of pulmonary tuberculosis, especially in the smear negative cases. One hundred and fifty suspected pulmonary TB patients (male/ female: 97/53) were included in this study. Single morning sputum was collected from each patient and diagnostic potential of PCR was compared with staining and culture. Twenty five (16.7%) sputum were positive by ZN stained smear. Among 125 smear negative samples, 13 (10.4%) yielded growth in culture in LJ media and 21 (16.8%) samples were positive by PCR. The sensitivity and specificity of PCR in smear negative cases was 100% and 92.9% respectively. Mean detection time in PCR was 24 hours. PCR detected M. tuberculosis in 21 smear negative and 9 culture negative samples. For diagnosis of tuberculosis in smear negative cases, PCR directly from sputum was a very sensitive and accurate method. In conclusion, PCR may be done, especially in clinically suspected pulmonary tuberculosis patients who remain negative by conventional methods.DOI: http://dx.doi.org/10.3329/bjmm.v6i2.19368 Bangladesh J Med Microbiol 2012; 06(02): 2-6

Aspirated teeth - rigid or flexible bronchoscopy

2019 ◽  
pp. 1-2
Keyword(s):  
Clinical Practice ◽  
Foreign Bodies ◽  
Motor Vehicle ◽  
Motor Vehicle Accident ◽  
Flexible Bronchoscopy ◽  
Vehicle Accident

Tracheobronchial foreign bodies are a common problem in clinical practice. We present the case of a patient with three aspirated teeth following a motor vehicle accident.

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