scholarly journals Antiangiogenic activity of zinc and zinc-sorafenib combination using the chick chorioallantoic membrane assay: A descriptive study

2020 ◽  
Vol 16 (8) ◽  
pp. 84
Author(s):  
GirishGulab Meshram ◽  
Manu Kumar ◽  
Tripti Rastogi ◽  
Sonal Sharma ◽  
Rachna Gupta ◽  
...  
Keyword(s):  
Chorioallantoic Membrane ◽  
Descriptive Study ◽  
Antiangiogenic Activity ◽  
Chick Chorioallantoic Membrane ◽  
Chorioallantoic Membrane Assay

scholarly journals Antiangiogenic activity of zinc and zinc-sorafenib combination using the chick chorioallantoic membrane assay: a descriptive study

2017 ◽  
Vol 6 (5) ◽  
pp. 1060
Author(s):  
Manu Kumar ◽  
Girish Gulab Meshram ◽  
Tripti Rastogi ◽  
Sonal Sharma ◽  
Rachna Gupta ◽  
...  
Keyword(s):  
Kinase Inhibitor ◽  
Chorioallantoic Membrane ◽  
Angiogenesis Inhibitor ◽  
Control Group ◽  
Cam Assay ◽  
Dose Ratio ◽  
Antiangiogenic Activity ◽  
Chick Chorioallantoic Membrane ◽  
Branching Points ◽  
Chorioallantoic Membrane Assay

Background: Zinc, a trace element, is known for downregulating several proangiogenic growth factors and cytokines. However, its antiangiogenic activity is not adequately studied. The present study was aimed to evaluate the possible antiangiogenic activity of zinc via the chick chorioallantoic membrane (CAM) assay. Also, the antiangiogenic activity of the combination therapy of zinc with various doses of sorafenib, a tyrosine kinase inhibitor, was evaluated.Methods: A pilot study was initially conducted so as to select suitable doses of zinc and sorafenib. The antiangiogenic activity after combining zinc 2.5 μg/embryo with sorafenib 1, and 2 μg/embryo was also evaluated. The antiangiogenic activity was quantified in terms of total length of blood vessels, number of junctions, number of branching points, and mean length of the blood vessels.Results: Zinc 2.5 μg/embryo showed significant (p <0.05) antiangiogenic activity, as compared to the control group. However, its effect was not comparable to that of sorafenib 2 μg/embryo. The combination of zinc 2.5 μg/embryo with sorafenib 2 μg/embryo did not show an additive/synergistic effect. The combination of zinc 2.5 μg/embryo with sorafenib 1 μg/embryo produced an antiangiogenic activity which was comparable (p >0.05) to that of sorafenib 2 μg/embryo.Conclusions: Zinc caused significant antiangiogenic activity in the CAM assay. The lack of addition/synergism in the zinc-sorafenib combination could have been due to the variability in the dose/ratio selection. Addition of zinc to sorafenib therapy could improve treatment tolerability, reduce cost of therapy, and reduce the emergence of drug resistance. Future mechanistic studies could identify the exact pharmacodynamics of zinc as an angiogenesis inhibitor.

scholarly journals Chick Chorioallantoic Membrane Assay: A 3D Animal Model for Study of Human Nasopharyngeal Carcinoma

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0130935 ◽  
Author(s):  
Xue Xiao ◽  
Xiaoying Zhou ◽  
Huixin Ming ◽  
Jinyan Zhang ◽  
Guangwu Huang ◽  
...  
Keyword(s):  
Animal Model ◽  
Nasopharyngeal Carcinoma ◽  
Chorioallantoic Membrane ◽  
Chick Chorioallantoic Membrane ◽  
Human Nasopharyngeal Carcinoma ◽  
Chorioallantoic Membrane Assay

scholarly journals Effect of miR-146a-5p on tumor growth in NSCLC using chick chorioallantoic membrane assay and bioinformatics investigation

2017 ◽  
Vol 16 (6) ◽  
pp. 8781-8792 ◽  
Author(s):  
Wen-Ting Huang ◽  
Wei-Luan Cen ◽  
Rong-Quan He ◽  
You Xie ◽  
Yu Zhang ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Chorioallantoic Membrane ◽  
Chick Chorioallantoic Membrane ◽  
Chorioallantoic Membrane Assay

scholarly journals Evolving applications of the egg: chorioallantoic membrane assay and ex vivo organotypic culture of materials for bone tissue engineering

2020 ◽  
Vol 11 ◽  
pp. 204173142094273
Author(s):  
Karen M Marshall ◽  
Janos M Kanczler ◽  
Richard OC Oreffo
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Organotypic Culture ◽  
Ex Vivo ◽  
Chorioallantoic Membrane ◽  
Experimental Methodology ◽  
Chick Chorioallantoic Membrane ◽  
Recent Developments ◽  
Chorioallantoic Membrane Assay

The chick chorioallantoic membrane model has been around for over a century, applied in angiogenic, oncology, dental and xenograft research. Despite its often perceived archaic, redolent history, the chorioallantoic membrane assay offers new and exciting opportunities for material and growth factor evaluation in bone tissue engineering. Currently, superior/improved experimental methodology for the chorioallantoic membrane assay are difficult to identify, given an absence of scientific consensus in defining experimental approaches, including timing of inoculation with materials and the analysis of results. In addition, critically, regulatory and welfare issues impact upon experimental designs. Given such disparate points, this review details recent research using the ex vivo chorioallantoic membrane assay and the ex vivo organotypic culture to advance the field of bone tissue engineering, and highlights potential areas of improvement for their application based on recent developments within our group and the tissue engineering field.

Sign in / Sign up

Export Citation Format

Share Document