Melengestrol acetate and estradiol-17β for synchronizing estrus and improving reproductive performance in beef cattle

1997 ◽  
Vol 77 (4) ◽  
pp. 683-688 ◽  
Author(s):  
J. P. Kastelic ◽  
D. H. McCartney ◽  
W. O. Olson ◽  
A. D. Barth ◽  
R. J. Mapletoft

Four experiments were conducted to investigate the use of melengesterol acetate (MGA) and estradiol-17β (E-17β) for synchronizing estrus and improving reproductive performance in beef cattle. Synchronized pregnancy rates were higher (P < 0.05) in heifers fed MGA after AI than controls (4 of 9 versus 0 of 7, respectively), but were not different for cows (20 of 37, 54% versus 23 of 40, 58%). Estrus rate (percentage of cows detected in estrus) was higher (P < 0.05) for cows treated with E-17β + MGA compared with those treated with cloprostenol alone (85.0 versus 65.9%). Synchronized pregnancy rates were not significantly affected by feeding MGA or grain only after detected estrus and AI (20 of 31, 64% versus 17 of 30, 57%). For 20 cows not detected in estrus and bred 100 h after cloprostenol treatment, pregnancy rate was higher (P < 0.05) for those fed MGA after AI than controls (6 of 11, 54% versus 1 of 9, 11%). Feeding MGA after breeding may help to improve pregnancy rates. Key words: Melengesterol acetate, estradiol-17β, estrus, reproductive performance, beef cattle

1999 ◽  
Vol 79 (1) ◽  
pp. 39-43 ◽  
Author(s):  
J. Thundathil ◽  
J. P. Kastelic ◽  
W. O. Olson ◽  
R. B. Cook ◽  
R. J. Mapletoft

Three experiments were conducted with suckled crossbred beef cows to determine the efficacy of various short-term regimens for synchronizing estrus or ovulation and to determine whether feeding melengestrol acetate (MGA) after timed AI will increase pregnancy rate. In exp. 1, all cows (n = 141) were given 0.5 mg MGA head−1 d−1 for 7 d (days 1 to 7), 1 or 5 mg E17β (and 100 mg progesterone) or 100 µg GnRH on day 1, and 500 µg cloprostenol on day 7. There were no significant differences among groups for estrous or synchronized conception rates (average, 84.4 and 52.1%, respectively). With an identical protocol in exp. 2 (78 cows), by day 11, cows given 5 mg E17β had the highest estrous and synchronized pregnancy rates (65.4 and 42.3%), compared to 1 mg E17β (46.2 and 15.4%) and GnRH (34.6 and 11.5%). Cows not detected in estrus were timed-inseminated and given 100 µg GnRH, 100 h after cloprostenol. There were no significant differences among groups for synchronized conception rate to timed AI (65.0%) or synchronized pregnancy rate to all inseminations (56.4%). Overall, 5 mg E17β gave the most consistent results. In exp. 3, 84 cows were given 100 µg GnRH on days 1 and 9, 500 µg cloprostenol on day 7, and were timed-inseminated on day 10. Half were fed MGA (0.5 mg head−1 d−1) on days 16 to 22, but the pregnancy rate was not different from that in the remaining cows (55.0 versus 47.8%, P > 0.5). Key words: Ovary, follicles, estrus synchronization, beef cows


1991 ◽  
Vol 71 (2) ◽  
pp. 589-591 ◽  
Author(s):  
R. N. Kirkwood ◽  
P. A. Thacker

One hundred gilts and one hundred mixed-parity sows of Yorkshire and Landrace breeding were inseminated with semen with or without an additional 10 μg estradiol-17β. Pregnancy rates of gilts receiving estradiol-supplemented and control semen were 90 and 79%, respectively (P < 0.09). The estradiol supplementation of semen did not improve the pregnancy rates or litter size in sows. Key words: Swine, artificial insemination, estradiol


1991 ◽  
Vol 71 (1) ◽  
pp. 237-239 ◽  
Author(s):  
P. A. Thacker ◽  
A. D. Gooneratne ◽  
R. N. Kirkwood

Pregnancy rates and litter sizes following insemination of sows with fresh-extended and frozen-thawed semen averaged 71 vs. 53% and 10.6 vs. 4.4, respectively, and was not affected by the addition of 12.5 μg relaxin to semen. These results indicate no relaxin mediated effect on either pregnancy rate or litter size when sows are artificially inseminated with relaxin-supplemented semen. Key words: Artificial insemination, relaxin, sows


1989 ◽  
Vol 69 (1) ◽  
pp. 39-46
Author(s):  
A. FARID ◽  
M. MAKARECHIAN ◽  
R. T. BERG ◽  
M. A. PRICE

Yearling bull fertility, measured as pregnancy rate, percentage of calves born during the first 4 and 6 wk of calving and mean and median of calving date distribution, were studied in 109 single-sire breeding herds of two breed groups over a 10-yr period. The breed groups were Herefords bulls mated to purebred and crossbred Hereford cows, and Beef Synthetic bulls mated to Beef Synthetic cows. The bulls averaged 14 mo old at the start of breeding. The average bull-to-cow ratio was approximately 1:22, and the breeding season started on 1 July each year and extended for 60 d. Two out of 109 bulls (1.8%) were subfertile (less than 50% pregnancy of the herd). The Beef Synthetic, which had crossbred foundation, had significantly higher fertility, calved earlier, had smaller phenotypic variance, and lower year-to-year fluctuation in the reproductive traits than the Hereford. Variations in reproductive performances of bulls of the same breed group used in the same year were much larger than those among different years, the former comprised over 75% of phenotypic variance of the traits in the two breed groups. Relationships between fertility of bulls and their weight and growth parameters were negligible, but bull fertility tended (P < 0.10) to improve with age. Bulls that were heavy at weaning, end of feedlot test and breeding settled their mates earlier during the breeding season. Key words: Beef cattle, yearling bull, fertility


2021 ◽  
Vol 53 (4) ◽  
Author(s):  
Hesam Kohsari ◽  
Khatereh Berenjian

This study was performed on an industrial dairy farm near Tehran province, Iran, on 666 lactating Holstein cows from September 2019 to June 2020 in order to eliminate the harmful effects of heat stress on the reproductive performance of the cows. The hypothesis of the recent study was that by increasing the serum concentration of progesterone (P4) in the luteal phase before insemination in the form of Presynch-Ovsynch+CIDR (POC) and G6G protocols, the pregnancy rate of cows would be increased compared to the Presynch-Ovsynch (PO) protocol. The aim of the present study was to evaluate the effects of Presynch-Ovsynch (PGF2α-14d- PGF2α-12d-Ovsynch (OVS: GnRH(GnRH1)- 7d-PGF2α(PG)-2d-GnRH)-18h-Fixed-time artificial insemination (FTAI), n=212), Presynch-Ovsynch+CIDR (similar to the PO protocol, plus the use of a CIDR for 7 days, from the start of the OVS+FTAI protocol to the time of PG injection, n=230), and G6G (PGF2α- 2d-GnRH-6d-OVS+FTAI, n=224) protocols on the reproductive performance and pregnancy rate of lactating Holstein cows. The average body condition score (BCS) for the cows was about 2.5 at the time of the onset of the OVS+FTAI program. The average daily milk production for cows at the time of the start of the OVS+FTAI program was 38.3 kg/day. On average, the studied cows were inseminated 86 days postpartum. Implementation of the G6G protocol in comparison to the PO and POC protocols increased the serum concentration of P4 at GnRH1 (P=0.04). The cows that received the G6G protocol had a greater number of corpuses luteum (CL) on their ovaries at PG in comparison to the cows in the PO and POC protocols (P=0.03 and P=0.05, respectively). For all treatment protocols, the pregnancy rate of cows with &gt;2 CLs on their ovaries at PG was significantly higher than for cows with ≤2 CLs on their ovaries at this time. The overall pregnancy rate for all cows studied was 42%. Separately, pregnancy rates for cows in PO, POC, and G6G protocols were calculated at 36.7%, 41.7%, and 47.3% respectively. The highest and the lowest pregnancy rates were obtained in the G6G and PO protocols, respectively (P=0.03). It can be concluded that the G6G protocol increased the pregnancy rate of cows in comparison to the PO but not to the POC protocol. This result can be attributed to the increase in serum concentration of P4 at GnRH1 and the greater number of CLs at PG following implementation of the G6G compared to the PO protocol. Although CIDR administration in the POC protocol led to an increase in the pregnancy rate of cows compared to the PO protocol, possibly by increasing the synchrony rate and the quality of the ovulatory follicle, this increase was not statistically significant (P=0.09).


Nativa ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 281-285
Author(s):  
Gabriel Rodrigo Hass Perucchi ◽  
Gabriela de Souza Sartori ◽  
Richarlla Aparecida Buscariol Silva ◽  
Murilo da Silva Garcia ◽  
Rodrigo José Delgado Jardim ◽  
...  

A Inseminação artificial em tempo fixo (IATF) está se difundindo no Brasil, pois contribui para o aumento da produtividade. O objetivo neste trabalho foi avaliar o uso do GnRH em protocolos de IATF de vacas criadas no pantanal Sul-mato-grossense. Participaram da pesquisa 531 vacas paridas da raça Nelore, que foram avaliadas de acordo com o escore de condição corporal (ECC), submetidas a três tipos de protocolos de IATF (lote 1, 2 e 3). Após 40 dias da inseminação artificial foi realizado diagnóstico de gestação. As matrizes que não receberam GnRH no lote 1 (estro aparente), lote 2 (estro parcial) e lote 3 (sem apresentação de estro) apresentaram, respectivamente 55,4%, 19,2% e 25,9% de prenhez, enquanto as que receberam o fármaco apresentaram 48,6%, 37,2% e 38,8%. Relacionado ao ECC, pode-se observar que a maior taxa de prenhez ocorreu em animais com ECC 3. Primíparas apresentaram melhor taxa de prenhez quando receberam GnRH independente do ECC. Concluiu-se que o uso de GnRH mostrou-se eficiente para melhoria da taxa de prenhez principalmente em primíparas, assim como nos animais que não apresentaram estro ou apresentaram estro parcial, e que matrizes com ECC 3 apresentaram melhores taxas de prenhez que vacas com escore inferior. Palavras-chave: bovino de corte; inseminação artificial em tempo fixo; produtividade; GnRH; Nelore.   Evaluation of cow reproductive performance in the region of Mato Grosso do Sul swamp submitted to TAI with GnRH application   ABSTRACT: The technique of timed artificial insemination (TAI) is spreading in Brazil, as it contributes to the increase of productivity. The objective of this work was to evaluate the use of GnRH in TAI protocols of cows raised in the swamp of Mato Grosso do Sul. 531 Nelore breed calved cows participate in the survey, which were evaluated according to the body condition score (BCS), and then performed three types of TAI protocols (batch 1, 2 and 3). After 40 days of the insemination, a pregnancy diagnosis was made. The matrices that did not receive GnRH in batch 1 (apparent oestrus), batch 2 (partial oestrus) and batch 3 (no oestrus presentation) presented respectively 55.4%, 19.2% and 25.9% of pregnancy, while those receiving the drug showed 48.6%, 37.2% and 38.8%. Related to BCS, it can be observed that the highest pregnancy rate occurred in animals with BCS 3. Primiparous had a better pregnancy rate when receiving GnRH regardless of BCS. It was concluded that the use of GnRH was efficient to improve the pregnancy rate mainly in primiparous as well as animals that showed no oestrus or partial oestrus, and matrices with BCS 3 had better pregnancy rates than cows with lower scores. Keywords: beef cattle; timed artificial insemination; productivity; GnRH; Nelore.


2019 ◽  
Vol 64 (No. 11) ◽  
pp. 459-464
Author(s):  
Jörn Rethmeier ◽  
Michael Wenzlau ◽  
Martin Wagner ◽  
Steffi Wiedemann ◽  
Lisa Bachmann

Fertility in dairy cows has decreased over the last fifty years while milk production per cow has increased. Furthermore, dairy herds become larger resulting in fewer employees per cow, which might also influence reproductive performance. To elucidate the situation in Germany, selected parameters (conception rate, service rate, pregnancy rate, days to first insemination and days open) were studied using data on 148 herds. For statistical analysis the herds were categorized concerning milk yield: (1) &lt; 30 kg, (2) 30‒35 kg, and (3) &gt; 35 kg/cow/day as well as concerning herd size: (1) &lt; 200, (2) 200‒400, (3) 400‒1000 and (4) &gt; 1000 milking cows. There was no difference in conception rate among herds. That means on dairy farms of larger size or with high milk yield the chance of an inseminated cow to become pregnant is the same as in small herds or in herds with low milk yield. Small herds (&lt; 200 cows) had lower pregnancy rates than larger herds (200–400 cows). The pregnancy rate in herds with different milk yield was not statistically different. Though there was a trend that dairy farms with higher milk yield (&gt; 30 kg) had higher pregnancy rates. The statistical differences and trends of pregnancy rates are due to higher service rate in larger herds as well as in herds with high milk yield. Therefore, service rate is the key factor for high reproductive performance on dairy farms. Poor fertility is not associated with high milk yield on herd basis or large herd size but may represent inappropriate farm management.


2011 ◽  
Vol 23 (1) ◽  
pp. 163 ◽  
Author(s):  
R. Núñez ◽  
T. de Castro ◽  
L. Cutaia ◽  
G. Bó ◽  
A. Menchaca

An important proportion of embryonic deaths in cattle occur during the critical period of embryo development and maternal recognition of pregnancy. High serum progesterone concentrations during this period are associated to higher embryo survival, and the use of eCG could enhance luteal activity. Two experiments were carried out to evaluate the effect of administering eCG at the time of removal of a progesterone-releasing device and 14 days after fixed-time artificial insemination (FTAI) in beef cattle. Multiparous suckled cows between 60 and 90 days postpartum (n = 297; Exp. 1), and two-year old prepuberal heifers (n = 689; Exp. 2) with a body condition score (BCS) between 3.5 and 5 (1 to 8 scale) were used. At the beginning of the experiments, none of females used had a corpus luteum detected by ultrasonography. Females received an intravaginal device containing 0.5 g progesterone (DIB 0.5, Syntex, Buenos Aires, Argentina) and 2 mg oestradiol benzoate i.m. (Benzoato de Oestradiol, Syntex). Eight days later, 500 μg of cloprostenol (Ciclase DL, Syntex) and 0.5 mg oestradiol cypionate (Cipiosyn, Syntex) were given i.m. and devices were removed; FTAI was performed 52 to 56 h after DIB removal. At the time of device removal, the animals were randomly allocated to 4 groups, to receive 400 IU of eCG i.m. (Novormon, Syntex) or placebo at device removal and/or 14 days after FTAI, in a 2 × 2 factorial design. Control group did not receive eCG. In Exp. 1, nose plates were placed on calves on the day of DIB insertion and left for 10 days in order to prevent suckling. Pregnancy diagnosis was performed by ultrasonography at 30 and 60 days after FTAI. Data were analysed by logistic regression. Pregnancy rate at 30 days after FTAI was affected by treatment (P < 0.05; Table 1). Late embryonic/fetal loss rates (non-pregnant females at 60 days after FTAI over those pregnant at 30 days) were 1.6% (3/182) for cows in Exp. 1 and 4.8% (14/292) for heifers in Exp. 2; no differences were detected among groups. In conclusion, administration of 400 IU of eCG at device removal increases pregnancy rate, both in anestrus cows and heifers. However, the administration of a second dose of eCG 14 days after FTAI suggests a possible effect on embryo survival within the first 30 days of pregnancy in cows but not in heifers. Further studies are required to confirm these results. Table 1.Pregnancy rates after administration of eCG at time of progesterone device removal and/or 14 days after insemination in beef cattle Supported by Syntex.


2001 ◽  
Vol 81 (3) ◽  
pp. 335-343 ◽  
Author(s):  
J. A. Small ◽  
J. D. Ambrose ◽  
W. P. McCaughey ◽  
D. R. Ward ◽  
W. D. Sutherland ◽  
...  

Trials were conducted in the spring (May; n = 324) and fall (October; n = 132) with crossbred continental-type beef cows assigned on the basis of parity and postpartum interval to one of three timed-AI treatments and one of two post-AI treatments. The timed-AI treatments were: (DPG) double (14 d apart) PGF2α (Lutalyse®) and AI (day = 0) 72 h after the second PGF2α (day –3); (OVS) Ovsynch® with the second GnRH (Factrel®) at 48 h and AI at 66 h; and (BRC) the same as OVS except that the second GnRH was given at the time of AI. Half of the cows within each treatment were given GnRH on day 14. Plasma progesterone concentrations were determined for the day of the first injection and on days –3, 0, 14, and 21. Timed-AI pregnancy was diagnosed by ultrasonography at day 42 and confirmed at calving. For DPG, OVS and BRC, PGF2α responder rates were 75.9, 51.4 and 71.3%, respectively, in spring (P < 0.05) and 70.4, 70.4 and 59.1% in fall (P > 0.05), and AI pregnancy rates were 28.7, 44.9 and 44.4% in spring (P< 0.05) and 25.0, 40.9 and 43.2% in fall (P > 0.05). Post-AI GnRH had no significant effect on pregnancy or conception rates or day 21 progesterone. The use of GnRH in the PGF2α based timed-AI program improved pregnancy rates and the BRC treatment was as effective as OVS. Neither postpartum interval nor initial progesterone concentration influenced (P >0.05) the effect of GnRH on AI pregnancy rate, and GnRH had no effect (P > 0.05) on twinning rate or gender ratio. Key words: Beef cows, estrous synchronization, pregnancy, timed-AI, progesterone


2015 ◽  
Vol 13 (2) ◽  
pp. 43-47
Author(s):  
P Myagmarsuren ◽  
U Myagmardulam ◽  
Ts Haliunaa

In all domestic species, acceptable pregnancy rate with frozen-thawed semen is 20-30% through simply cervical and vaginal insemination. Researchers have proved that achievable high results can be expected only from intrauterine insemination in ewes. Unlike the cow, and goat, the cervix of ewes is a formidable barrier to penetrate for transcervical intrauterine insemination. It is not only due to the physical size of the canal and a narrow luminal diameter (even at estrus), but also due to its caudally facing eccentric series of four to eight funnel-like rings. The major aim of this study was to evaluate a comparison of transcervical and laparoscopic intrauterine artificial insemination techniques with frozen –thawed semen on the reproductive performance of ewes.58ewes of 3-4 years of age, so called Altanbulag subspecies of breed were selected and equally divided into 2 groups for the AI methods from October 15-20, 2013 in the breeding station of Research Institute of Animal Husbandry in Hongor sum, Darhan uul province. The estrus was synchronized with EAZI-BREED TMCIDR in which the progesterone content was 0.3 g. After 12 days, the CIDR were removed, PMSG of 400 and 200 IU were administrated intramuscularly per ewe. The total success of synchronization rate was 93.3%. The results has shown the possibilities of laparoscopic and transcervical AI techniques in 55.17%, 41.37% (p>0.05) of pregnancy rates respectively, with frozen-thawed semen in ewes by ultrasonography diagnosis.Mongolian Journal of Agricultural Sciences Vol.13(2) 2014: 43-47


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