RESULT OF ADVANCED TECHNIQUES FOR ARTIFICIAL INSEMINATION IN EWES WITH FROZEN-THAWED SEMEN

2015 ◽  
Vol 13 (2) ◽  
pp. 43-47
Author(s):  
P Myagmarsuren ◽  
U Myagmardulam ◽  
Ts Haliunaa
Keyword(s):  
Pregnancy Rate ◽  
Reproductive Performance ◽  
Artificial Insemination ◽  
Intrauterine Insemination ◽  
Animal Husbandry ◽  
Pregnancy Rates ◽  
Luminal Diameter ◽  
Physical Size ◽  
Domestic Species ◽  
Breeding Station

In all domestic species, acceptable pregnancy rate with frozen-thawed semen is 20-30% through simply cervical and vaginal insemination. Researchers have proved that achievable high results can be expected only from intrauterine insemination in ewes. Unlike the cow, and goat, the cervix of ewes is a formidable barrier to penetrate for transcervical intrauterine insemination. It is not only due to the physical size of the canal and a narrow luminal diameter (even at estrus), but also due to its caudally facing eccentric series of four to eight funnel-like rings. The major aim of this study was to evaluate a comparison of transcervical and laparoscopic intrauterine artificial insemination techniques with frozen –thawed semen on the reproductive performance of ewes.58ewes of 3-4 years of age, so called Altanbulag subspecies of breed were selected and equally divided into 2 groups for the AI methods from October 15-20, 2013 in the breeding station of Research Institute of Animal Husbandry in Hongor sum, Darhan uul province. The estrus was synchronized with EAZI-BREED TMCIDR in which the progesterone content was 0.3 g. After 12 days, the CIDR were removed, PMSG of 400 and 200 IU were administrated intramuscularly per ewe. The total success of synchronization rate was 93.3%. The results has shown the possibilities of laparoscopic and transcervical AI techniques in 55.17%, 41.37% (p>0.05) of pregnancy rates respectively, with frozen-thawed semen in ewes by ultrasonography diagnosis.Mongolian Journal of Agricultural Sciences Vol.13(2) 2014: 43-47

The influence of purified porcine relaxin on the reproductive performance of sows following artificial insemination with fresh or frozen semen

1991 ◽  
Vol 71 (1) ◽  
pp. 237-239 ◽  
Author(s):  
P. A. Thacker ◽  
A. D. Gooneratne ◽  
R. N. Kirkwood
Keyword(s):  
Key Words ◽  
Pregnancy Rate ◽  
Litter Size ◽  
Reproductive Performance ◽  
Artificial Insemination ◽  
Pregnancy Rates ◽  
Frozen Semen

Pregnancy rates and litter sizes following insemination of sows with fresh-extended and frozen-thawed semen averaged 71 vs. 53% and 10.6 vs. 4.4, respectively, and was not affected by the addition of 12.5 μg relaxin to semen. These results indicate no relaxin mediated effect on either pregnancy rate or litter size when sows are artificially inseminated with relaxin-supplemented semen. Key words: Artificial insemination, relaxin, sows

scholarly journals The Effect of A PRID®Delta-Based Estrus Synchronization Protocol On Pregnancy Rates In Cows With No Estrus Symptoms

2022 ◽  
Author(s):  
Murat Can Demir ◽  
Cihan Kaçar ◽  
Umut Çağın Arı ◽  
Semra Kaya ◽  
Oğuz Merhan ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Prostaglandin F2α ◽  
Pregnancy Rates ◽  
Dominant Follicle ◽  
Serum Progesterone ◽  
Blood Samples ◽  
Follicle Diameter ◽  
Follicle Size ◽  
Equine Chorionic Gonadotropin

Abstract The present study aimed to determine the effects of progesterone-based gonadotropin-releasing hormone (GnRH), prostaglandin F2α (PGF2α), and equine chorionic gonadotropin (eCG) injections on progesterone profiles and pregnancy rates in cows with no estrus symptoms within 60 days after parturition. A total of 80 cows were included in the study. All animals had the progesterone-releasing device PRID®Delta placed intravaginally for nine days with an injection of GnRH. On the eighth day, PGF2α was injected, and PRID®Delta was removed from the vagina on day nine. Artificial insemination was carried out 60 hours after PRID®Delta removal. In half of the animals (n = 40), 600 IU of eCG was injected when PRID®Delta was removed on the ninth day before artificial insemination 60 hours later. Blood samples were taken from the tail vein on days 0 and 8 to determine progesterone levels. The pregnancy rate in the group that received eCG was 37.5%, while it was 27.5% in those that did not (P = 0.4). While the dominant follicle diameter was 15.5 mm in cows injected with eCG during timed artificial insemination, the follicle diameter was 12.4 mm in cows with no eCG injection (P <0.001). There were no differences in serum progesterone values in blood samples taken until the time of artificial insemination. However, progesterone values in the blood taken during artificial insemination were 0.94 ng/ml in the eCG- group and 0.72 ng/ml in the eCG+ group (P <0.05). As a result, it was determined that eCG injections, in addition to progesterone-based GnRH and PGF2α applications, increased the pregnancy rates in cows without symptoms of estrus. The dominant follicle diameter was larger in cows treated with eCG during artificial insemination; however, follicle size did not increase the pregnancy rate.

scholarly journals Effect of progesterone, human chorionic gonadotropin and progesterone + human chorionic gonadotropin treatment on conception rates in repeat breeder cows

2020 ◽  
Vol 89 (4) ◽  
pp. 307-315
Author(s):  
Hasan Alkan ◽  
Huseyin Erdem
Keyword(s):  
Pregnancy Rate ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Artificial Insemination ◽  
Fixed Time ◽  
Pregnancy Rates ◽  
Group 4 ◽  
Prostaglandin F ◽  
Repeat Breeder ◽  
Group 1

The aim of this study was to investigate the effects of hormonal support on the pregnancy rate in repeat breeder cows. Prostaglandin F2α + Ovsynch oestrus synchronization protocol was applied to the cows. Following the fixed time insemination (day 0), the cows were divided into 4 groups. In Group 1 (n = 42), progesterone releasing intravaginal device (PRID) was placed vaginally at 84 h and removed on the 9th day after the artificial insemination. In Group 2 (n = 40), the cows were administered human chorionic gonadotropin (hCG) on the 7th day. Group 3 (n = 45) was applied a combination of progesterone and hCG. Group 4 (n = 42) was not given any treatment. Blood samples were collected from all cows 4 times on days 3.5, 7, 12, and 18 to evaluate progesterone concentrations. The pregnancy rates were 40.47%, 37.50%, 44.44%, and 30.95% in Group 1, 2, 3, and 4, respectively (P > 0.05). In addition, in cows with progesterone concentrations <2 ng/ml on day 3.5, the pregnancy rates were found to be lower than in the cows with progesterone concentrations >2 ng/ml in Group 4 (P < 0.05). Progesterone supplementation in cows with progesterone concentrations < 2 ng/ml appeared to increase pregnancy rates (P < 0.05) in Groups 1 and 3. As a result, post-insemination hormonal applications in the repeat breeder cows did not increase the pregnancy rate. However, it was concluded that determination of progesterone concentrations on day 3.5 following artificial insemination and then hormonal support in the cows with low concentrations would increased the pregnancy rate.

scholarly journals Comparison of the effects of Presynch-Ovsynch, Presynch- Ovsynch+CIDR, and G6G protocols on the reproductive performance of lactating Holstein cows in the postpartum period

2021 ◽  
Vol 53 (4) ◽  
Author(s):  
Hesam Kohsari ◽  
Khatereh Berenjian
Keyword(s):  
Serum Concentration ◽  
Pregnancy Rate ◽  
Reproductive Performance ◽  
Body Condition Score ◽  
Dairy Farm ◽  
Fixed Time ◽  
Pregnancy Rates ◽  
Holstein Cows ◽  
Treatment Protocols ◽  
Ovulatory Follicle

This study was performed on an industrial dairy farm near Tehran province, Iran, on 666 lactating Holstein cows from September 2019 to June 2020 in order to eliminate the harmful effects of heat stress on the reproductive performance of the cows. The hypothesis of the recent study was that by increasing the serum concentration of progesterone (P4) in the luteal phase before insemination in the form of Presynch-Ovsynch+CIDR (POC) and G6G protocols, the pregnancy rate of cows would be increased compared to the Presynch-Ovsynch (PO) protocol. The aim of the present study was to evaluate the effects of Presynch-Ovsynch (PGF2α-14d- PGF2α-12d-Ovsynch (OVS: GnRH(GnRH1)- 7d-PGF2α(PG)-2d-GnRH)-18h-Fixed-time artificial insemination (FTAI), n=212), Presynch-Ovsynch+CIDR (similar to the PO protocol, plus the use of a CIDR for 7 days, from the start of the OVS+FTAI protocol to the time of PG injection, n=230), and G6G (PGF2α- 2d-GnRH-6d-OVS+FTAI, n=224) protocols on the reproductive performance and pregnancy rate of lactating Holstein cows. The average body condition score (BCS) for the cows was about 2.5 at the time of the onset of the OVS+FTAI program. The average daily milk production for cows at the time of the start of the OVS+FTAI program was 38.3 kg/day. On average, the studied cows were inseminated 86 days postpartum. Implementation of the G6G protocol in comparison to the PO and POC protocols increased the serum concentration of P4 at GnRH1 (P=0.04). The cows that received the G6G protocol had a greater number of corpuses luteum (CL) on their ovaries at PG in comparison to the cows in the PO and POC protocols (P=0.03 and P=0.05, respectively). For all treatment protocols, the pregnancy rate of cows with &gt;2 CLs on their ovaries at PG was significantly higher than for cows with ≤2 CLs on their ovaries at this time. The overall pregnancy rate for all cows studied was 42%. Separately, pregnancy rates for cows in PO, POC, and G6G protocols were calculated at 36.7%, 41.7%, and 47.3% respectively. The highest and the lowest pregnancy rates were obtained in the G6G and PO protocols, respectively (P=0.03). It can be concluded that the G6G protocol increased the pregnancy rate of cows in comparison to the PO but not to the POC protocol. This result can be attributed to the increase in serum concentration of P4 at GnRH1 and the greater number of CLs at PG following implementation of the G6G compared to the PO protocol. Although CIDR administration in the POC protocol led to an increase in the pregnancy rate of cows compared to the PO protocol, possibly by increasing the synchrony rate and the quality of the ovulatory follicle, this increase was not statistically significant (P=0.09).

scholarly journals AVALIAÇÃO DO DESEMPENHO REPRODUTIVO DE VACAS NA REGIÃO DO PANTANAL SUL-MATO-GROSSENSE SUBMETIDAS A IATF Com Aplicação De GnRH

Nativa ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 281-285
Author(s):  
Gabriel Rodrigo Hass Perucchi ◽  
Gabriela de Souza Sartori ◽  
Richarlla Aparecida Buscariol Silva ◽  
Murilo da Silva Garcia ◽  
Rodrigo José Delgado Jardim ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Pregnancy Rate ◽  
Reproductive Performance ◽  
Artificial Insemination ◽  
Body Condition Score ◽  
The Body ◽  
Mato Grosso ◽  
Condition Score ◽  
Timed Artificial Insemination ◽  
Mato Grosso Do Sul

A Inseminação artificial em tempo fixo (IATF) está se difundindo no Brasil, pois contribui para o aumento da produtividade. O objetivo neste trabalho foi avaliar o uso do GnRH em protocolos de IATF de vacas criadas no pantanal Sul-mato-grossense. Participaram da pesquisa 531 vacas paridas da raça Nelore, que foram avaliadas de acordo com o escore de condição corporal (ECC), submetidas a três tipos de protocolos de IATF (lote 1, 2 e 3). Após 40 dias da inseminação artificial foi realizado diagnóstico de gestação. As matrizes que não receberam GnRH no lote 1 (estro aparente), lote 2 (estro parcial) e lote 3 (sem apresentação de estro) apresentaram, respectivamente 55,4%, 19,2% e 25,9% de prenhez, enquanto as que receberam o fármaco apresentaram 48,6%, 37,2% e 38,8%. Relacionado ao ECC, pode-se observar que a maior taxa de prenhez ocorreu em animais com ECC 3. Primíparas apresentaram melhor taxa de prenhez quando receberam GnRH independente do ECC. Concluiu-se que o uso de GnRH mostrou-se eficiente para melhoria da taxa de prenhez principalmente em primíparas, assim como nos animais que não apresentaram estro ou apresentaram estro parcial, e que matrizes com ECC 3 apresentaram melhores taxas de prenhez que vacas com escore inferior. Palavras-chave: bovino de corte; inseminação artificial em tempo fixo; produtividade; GnRH; Nelore.   Evaluation of cow reproductive performance in the region of Mato Grosso do Sul swamp submitted to TAI with GnRH application   ABSTRACT: The technique of timed artificial insemination (TAI) is spreading in Brazil, as it contributes to the increase of productivity. The objective of this work was to evaluate the use of GnRH in TAI protocols of cows raised in the swamp of Mato Grosso do Sul. 531 Nelore breed calved cows participate in the survey, which were evaluated according to the body condition score (BCS), and then performed three types of TAI protocols (batch 1, 2 and 3). After 40 days of the insemination, a pregnancy diagnosis was made. The matrices that did not receive GnRH in batch 1 (apparent oestrus), batch 2 (partial oestrus) and batch 3 (no oestrus presentation) presented respectively 55.4%, 19.2% and 25.9% of pregnancy, while those receiving the drug showed 48.6%, 37.2% and 38.8%. Related to BCS, it can be observed that the highest pregnancy rate occurred in animals with BCS 3. Primiparous had a better pregnancy rate when receiving GnRH regardless of BCS. It was concluded that the use of GnRH was efficient to improve the pregnancy rate mainly in primiparous as well as animals that showed no oestrus or partial oestrus, and matrices with BCS 3 had better pregnancy rates than cows with lower scores. Keywords: beef cattle; timed artificial insemination; productivity; GnRH; Nelore.

scholarly journals The influence of adding estradiol to semen on reproductive performance of sows

1991 ◽  
Vol 71 (2) ◽  
pp. 589-591 ◽  
Author(s):  
R. N. Kirkwood ◽  
P. A. Thacker
Keyword(s):  
Key Words ◽  
Litter Size ◽  
Reproductive Performance ◽  
Artificial Insemination ◽  
Pregnancy Rates ◽  
And Control ◽  
Estradiol 17Β

One hundred gilts and one hundred mixed-parity sows of Yorkshire and Landrace breeding were inseminated with semen with or without an additional 10 μg estradiol-17β. Pregnancy rates of gilts receiving estradiol-supplemented and control semen were 90 and 79%, respectively (P < 0.09). The estradiol supplementation of semen did not improve the pregnancy rates or litter size in sows. Key words: Swine, artificial insemination, estradiol

scholarly journals Fertility parameters in German dairy herds: Associations with milk yield and herd size

2019 ◽  
Vol 64 (No. 11) ◽  
pp. 459-464
Author(s):  
Jörn Rethmeier ◽  
Michael Wenzlau ◽  
Martin Wagner ◽  
Steffi Wiedemann ◽  
Lisa Bachmann
Keyword(s):  
Pregnancy Rate ◽  
Milk Yield ◽  
Reproductive Performance ◽  
Dairy Farms ◽  
Herd Size ◽  
Pregnancy Rates ◽  
Conception Rate ◽  
Service Rate ◽  
Dairy Herds ◽  
Key Factor

Fertility in dairy cows has decreased over the last fifty years while milk production per cow has increased. Furthermore, dairy herds become larger resulting in fewer employees per cow, which might also influence reproductive performance. To elucidate the situation in Germany, selected parameters (conception rate, service rate, pregnancy rate, days to first insemination and days open) were studied using data on 148 herds. For statistical analysis the herds were categorized concerning milk yield: (1) &lt; 30 kg, (2) 30‒35 kg, and (3) &gt; 35 kg/cow/day as well as concerning herd size: (1) &lt; 200, (2) 200‒400, (3) 400‒1000 and (4) &gt; 1000 milking cows. There was no difference in conception rate among herds. That means on dairy farms of larger size or with high milk yield the chance of an inseminated cow to become pregnant is the same as in small herds or in herds with low milk yield. Small herds (&lt; 200 cows) had lower pregnancy rates than larger herds (200–400 cows). The pregnancy rate in herds with different milk yield was not statistically different. Though there was a trend that dairy farms with higher milk yield (&gt; 30 kg) had higher pregnancy rates. The statistical differences and trends of pregnancy rates are due to higher service rate in larger herds as well as in herds with high milk yield. Therefore, service rate is the key factor for high reproductive performance on dairy farms. Poor fertility is not associated with high milk yield on herd basis or large herd size but may represent inappropriate farm management.

Artificial insemination of farmed red deer (Cervus elaphus)

1990 ◽  
Vol 51 (3) ◽  
pp. 613-621 ◽  
Author(s):  
P. F. Fennessy ◽  
C. G. Mackintosh ◽  
G. H. Shackell
Keyword(s):  
Pregnancy Rate ◽  
Cervus Elaphus ◽  
Artificial Insemination ◽  
Red Deer ◽  
Pregnancy Rates ◽  
Progesterone Treatment ◽  
Serum Gonadotropin ◽  
The Difference ◽  
Fixed Times ◽  
In Pregnancy

ABSTRACTSix experiments involving the artificial insemination (AI) of a total of 300 female red deer (hinds) with frozen-thawed red deer semen (collected by electro-ejaculation) were conducted over 3 years. Insemination took place at fixed times following various oestrous synchronization procedures using progesterone withdrawal and treatment with pregnant mare's serum gonadotropin (PMSG). In the 1st year, the experiments evaluated basic AI techniques in which pregnancy rates were 45% in 20 hinds receiving two inseminations per vaginam (PV) and 56% in 27 hinds inseminated by the laparoscopic intrauterine method (IU). In the 2nd year, the experiments involved comparisons of the progesterone regime, one or two PV inseminations, and the timing of a single PV insemination. There was no effect of replacement of the progesterone device after 9 days and withdrawal 3 days later compared with the use of one device for the whole period in two experiments. The pregnancy rate for a double PV insemination was significantly higher than for a single PV insemination (58 and 34%; P < 0·05) and there was also a small effect of timing of insemination relative to the synchronization treatment. In the 3rd year all hinds were inseminated by the IU method. The experiments involved a comparison of various times of AI following progesterone withdrawal and a comparison of the progesterone regime. The overall pregnancy rate for 63 hinds inseminated was 56% with no difference between three times of insemination (48, 52 and 55 h). In the second experiment, the difference in pregnancy rate between treatment with progesterone for 15 days and 12 days (44 and 72% for 18 hinds per group) was not significant, but the interaction between the length of progesterone treatment and insemination time was significant (P < 0·05), with the 12-day progesterone/55 h insemination giving a much higher pregnancy rate than the 15-day/55 h insemination (89 and 20% respectively). Although no experiments involved direct comparisons of the routes of insemination, overall pregnancy rates were 56% for IU, 53% for double PV and 35% for single PV inseminations.

scholarly journals Economics of Improved Reproductive Performance in Dairy Cattle

EDIS ◽  
2019 ◽  
Vol 2005 (9) ◽  
Author(s):  
Albert De Vries ◽  
Jessika Van Leeuwen ◽  
William W. Thatcher
Keyword(s):  
Pregnancy Rate ◽  
Reproductive Performance ◽  
Economic Value ◽  
Economic Benefits ◽  
Cooperative Extension Service ◽  
Pregnancy Rates ◽  
Dairy Herds ◽  
University Of Florida ◽  
Fact Sheet ◽  
The Cost

This fact sheet provides some estimates of the economic benefits of improved reproductive performance through increasing pregnancy rates in dairy herds. After a short literature review, the fact sheet focuses on estimates under conditions in Florida. At the herd level, the economic value of an improvement in pregnancy rate and the cost of an additional day open are estimated. This is done for both an overall increase of pregnancy rate and an increase of first service pregnancy rate. In addition, the fact sheet presents some estimates of the maximum that can be spent on a reproductive program to obtain increases in first service pregnancy rates. At the cow level, the values of a new or established pregnancy are discussed. Results show the maximum that can be spent to get specific cows pregnant. This document is AN156, one of a series of the Department of Animal Sciences, Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida. Published August 2005.

scholarly journals Sperm concentration on the intrauterine artificial insemination in swine

2009 ◽  
Vol 33 (6) ◽  
pp. 1671-1674
Author(s):  
Joana Miller ◽  
Ana Luísa Neves Alvarenga ◽  
Luis David Solis Murgas ◽  
Adriana Cristina da Silva ◽  
Ricardo Sales Araújo ◽  
...  
Keyword(s):  
Litter Size ◽  
Reproductive Performance ◽  
Artificial Insemination ◽  
Intrauterine Insemination ◽  
Sperm Concentration ◽  
Conception Rate ◽  
Cervical Canal ◽  
Significant Difference ◽  
Estrus Detection ◽  
Farrowing Rate

The aim of this work was to evaluate the efficiency of the intrauterine insemination (IUI) in swine, considering the conception rate, farrowing rate, litter size (alive born pigs). For the IUI, the females had been insemination at 24 and 48 hours after the estrus detection, and the inseminating doses of 500 million, 1 billion, 1.5 billion and 2 billion spermatozoa in 20 mL extender had been used. The procedure of catheter insertion through the cervical canal was successfully performed in 97.9% of the females. The conception rate was 6.3% in the IUI. The farrowing rate in IUI was 87.2% but the farrowing rate was 100% for the sperm concentration of 500 million. Regarding the number of born pigs and alive born pigs observed in females inseminated with IUI, no significant difference was observed (p > 0.05). The concentration of 500 x 10(6) spermatozoa in 20 mL extender in the intrauterine insemination resulted in an optimal reproductive performance.

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