Field studies were conducted in summerfallow in 1990 and 1991 at Bezanson, Alberta (lat. 55°14′N, long. 118°22′W), and Melfort, Saskatchewan (lat. 52°52′N, long. 104°36′W), to compare the development of Canada thistle [Cirsium arvense (L.) Scop.] shoots following the discontinuance of normal tillage at three times (19 July, 30–31 July and 9–12 Aug.), at three depths (0, 10 and 20 cm), and its response to glyphosate applied at 0.9 kg ha−1 40 d after the last tillage (DALT). The tillage treatments had no consistent effect on shoot density when measured at 40 DALT at either location. At Bezanson, small increases (< 5%) in shoot density occurred between 20 and 40 DALT where tillage was conducted at the shallowest depth, but increases of approximately 20–90% occurred during the same period following tillage at the 10 or 20-cm depth. The proportion of emerged shoots remaining as rosettes at 40 DALT increased as the time of discontinuing tillage was delayed. At Bezanson, the proportion of emerged shoots remaining as rosettes at 40 DALT increased from 0.17 ± 0.05 to 0.59 ± 0.02 when the time of discontinuing tillage was delayed from mid-July to early August, while at Melfort an increase from 0.47 ± 0.04 to 0.93 ± 0.02 occurred for a similar delay in time of discontinuing tillage. Increasing the depth of last tillage increased the proportion of emerged shoots remaining as rosettes at 40 DALT at Bezanson, but at Melfort the depth of last tillage had no effect on this proportion. The proportion of shoots remaining as rosettes declined between 20 and 40 DALT when measured at Bezanson. Factors causing an increase in the proportion of shoots remaining as rosettes appeared to be associated with exposure of emerged shoots to reduced daylengths. At both Bezanson and Melfort, shoot density measured 1 yr after tillage–glyphosate treatments was similar in all plots. Thus, the proportion of shoots remaining as rosettes at the time of glyphosate application did not appear to influence the response of Canada thistle to the herbicide. Key words: Integrated weed control, daylength, rosette, perennial-weed control