scholarly journals Easy method for measuring insecticide susceptibility of five lepidopteran species using artificial diet

2021 ◽  
Vol 63 (0) ◽  
pp. 33-38
Author(s):  
Takeo Imura
Keyword(s):  
Artificial Diet ◽  
Insecticide Susceptibility ◽  
Easy Method ◽  
Lepidopteran Species

scholarly journals Susceptibility of Grapholita molesta to insecticides in Brazil

2017 ◽  
Vol 48 (1) ◽  
Author(s):  
Oscar Arnaldo Batista Neto e Silva ◽  
Marcos Botton ◽  
Daniel Bernardi ◽  
Cristiano João Arioli ◽  
Celso Omoto
Keyword(s):  
Insect Resistance ◽  
High Mortality ◽  
Artificial Diet ◽  
Resistance Management ◽  
Main Tool ◽  
Laboratory Population ◽  
High Susceptibility ◽  
Grapholita Molesta ◽  
Insecticide Susceptibility ◽  
Resistance Monitoring

ABSTRACT: The use of insecticides has been the main tool for Grapholita molesta (Busck) control in Brazil, which is considered one of the most important pests in apple and peach orchards. In order to implement an Insect Resistance Management (IRM) program, studies were conducted to characterize the baseline susceptibility of G. molesta to major insecticides for its control. Then, we conducted an insecticide susceptibility monitoring in thirteen field-collected populations of the pest. Neonates (0-24h old) were exposed to insecticides applied on surface of artificial diet. A high susceptibility was verified when neonates of the Laboratory population of G. molesta were exposed to insecticides with LC50 values (µg a.i./cm2) of 0.1 (spinetoram), 1.0 (metaflumizone), 1.2 (chlorantraniliprole), 4.8 (novaluron), 5.1 (tebufenozide), 11.3 (phosmet) and 222.5 (pyriproxyfen). Based on the LC99 (µg a.i./cm2), the diagnostic concentrations of 0.6 (spinetoram), 5.5 (metaflumizone), 5.6 (chlorantraniliprole), 19.6 (tebufenozide), 37.4 (phosmet), 37.8 (novaluron) and 2011 pyriproxyfen) caused high mortality (>95%) of neonates from field populations. These diagnostic concentrations will be used in resistance monitoring programs of G. molesta in Brazil.

A New Technique for the Light and Electron Microscopic Study of Individual Cerebro-Spinal Fluid Cells in a Mona Plane Layer

1976 ◽  
Vol 34 ◽  
pp. 362-363
Author(s):  
L.A. Dell
Keyword(s):  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Plane Layer ◽  
Ultrastructural Level ◽  
Spinal Fluid ◽  
Electron Microscopic Level ◽  
Electron Microscopic ◽  
Easy Method ◽  
Cell Pellet ◽  
A New Technique

A new method has been developed which readily offers the microscopist a possibility for both light and electron microscopic study of selected cells from the cerebrospinal fluid. Previous attempts to examine these cells in the spinal fluid at the ultrastructural level were based on modifications of cell pellet techniques developed for peripheral blood. These earlier methods were limited in application by the number of cells in spinal fluid required to obtain a sufficient size pellet and by the lack of an easy method of cellular identification between the light and electron microscopic level. The newly developed method routinely employs microscope slides coated with Siliclad and tungsten oxide for duplicate cytocentrifuge preparations of diagnostic spinal fluid specimens. Work done by Kushida and Suzuki provided a basis for our use of the metal oxide.

A method for rearing Arhopalus ferus (Mulsant) (Coleoptera Cerambycidae) larvae on a modified artificial diet

2015 ◽  
Vol 68 ◽  
pp. 353-359 ◽  
Author(s):  
A.M. Barrington ◽  
D.P. Logan ◽  
P.G. Connolly
Keyword(s):  
Introduced Species ◽  
Artificial Diet ◽  
Larval Density ◽  
Pine Bark ◽  
Pine Wood ◽  
Rearing Method ◽  
Sawn Timber

Burnt pine longhorn (BPL) Arhopalus ferus (Mulsant) (Coleoptera Cerambycidae) is an introduced species sometimes found in association with export logs and sawn timber A rearing method was developed to produce larvae of a known age number and quality for control trials Growth of larvae from newly hatched to 5 weeks was measured on a standard cerambycid artificial diet and on modified diets Replacing pine wood with pine bark sawdust increased survival at 5 weeks from 23 to 76 and mean weight from 9 to 21 mg There were significant interactions between the influences of three factors (diet period of rearing initial larval density) on the weight of surviving larvae Individual rearing was preferred for convenience and a standardised method was used to rear 8740 larvae for disinfestation trials Establishment and survival to 6 weeks for these larvae was 97

Insecticide Susceptibility Status of Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in Awka South Local Government Area, Anambra State, Nigeria

2019 ◽  
Vol 8 (3) ◽  
Author(s):  
Nwankwo EN ◽  
Ononye IP ◽  
Ogbonna CU ◽  
Ezihe EK ◽  
Onwude CO ◽  
...  
Keyword(s):  
Local Government ◽  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Local Government Area ◽  
Insecticide Susceptibility ◽  
Anambra State ◽  
Susceptibility Status

Indirect Determination of Amikacin by Gold Nanoparticles as Redox Probe

2020 ◽  
Vol 17 ◽  
Author(s):  
Mansureh Alizadeh ◽  
Mandana Amiri ◽  
Abolfazl Bezaatpour
Keyword(s):  
Gold Nanoparticles ◽  
Bacterial Infections ◽  
Limit Of Detection ◽  
Pharmaceutical Preparation ◽  
Cathodic Peak ◽  
Easy Method ◽  
Peak Current ◽  
Indirect Determination ◽  
Tract Infections

: Amikacin is an aminoglycoside antibiotic used for many gram-negative bacterial infections like infections in the urinary tract, infections in brain, lungs and abdomen. Electrochemical determination of amikacin is a challenge in electroanalysis because it shows no voltammetric peak at the surface of bare electrodes. In this approach, a very simple and easy method for indirect voltammetric determination of amikacin presented in real samples. Gold nanoparticles were electrodeposited at the surface of glassy carbon electrode in constant potential. The effect of several parameters such as time and potential of deposition, pH and scan rates on signal were studied. The cathodic peak current of Au3+ decreased with increasing amikacin concentration. Quantitative analysis of amikacin was performed using differential pulse voltammetry by following cathodic peak current of gold ions. Two dynamic linear ranges of 1.0 × 10−8–1.0 × 10-7 M and 5.0 × 10−7–1.0 × 10-3 M were obtained and limit of detection was estimated 3.0× 10−9 M. The method was successfully determined amikacin in pharmaceutical preparation and human serum. The effect of several interference in determination of amikacin was also studied.

Synthesis of a New Dioxaspiro[bicyclo[3.3.1]nonane-oxabicyclo[6.2.0]deca- 1(10),8-dien-4-one Derivative Using Some Chemical Strategies

2020 ◽  
Vol 17 (5) ◽  
pp. 393-402
Author(s):  
Figueroa-Valverde Lauro ◽  
Rosas-Nexticapa Marcela ◽  
Lopez-Ramos Maria ◽  
Diaz Cedillo Francisco ◽  
Mateu-Armand Virginia ◽  
...  
Keyword(s):  
Elemental Analysis ◽  
Chemical Structure ◽  
Spectroscopic Techniques ◽  
Easy Method ◽  
Chemical Strategies

There are several protocols for the preparation of bicyclic derivatives; however, some methods use dangerous and require special conditions. The aim of this study was to synthesize a new Dioxaspiro[ bicyclo[3.3.1]nonane-oxabicyclo[6.2.0]-deca-1(10), 8-dien-4-one (compound 8). Compound 8 was prepared using some reactions such as; i) etherification, ii) reduction, iii) amidation, iv) imination and v) 2+2 addition. The chemical structure of 8 and its intermediaries were completely characterized by spectroscopic techniques and elemental analysis. The synthesis showed a yield of 85% for compound 8. In this study, an easy method for the preparation of compound 8 is reported.

An Easy Efficient Method of Veterinary Drug Residue Analysis in Raw Milk by RP-HPLC-UV with Application to Raw Milk

2020 ◽  
Vol 16 (7) ◽  
pp. 942-949
Author(s):  
Lekweiri Haiba Legrae ◽  
Mohamed Fadel Deida ◽  
Bah Mohamed Lemine Abdellahi ◽  
Mohamed Brahim Elkory ◽  
Ibrahima Ndiaye ◽  
...  
Keyword(s):  
Residue Analysis ◽  
Raw Milk ◽  
Cow Milk ◽  
Veterinary Drug ◽  
Easy Method ◽  
Rp Hplc ◽  
Peak Resolution ◽  
European Standards ◽  
Moderate Cost ◽  
Short Time

Background: This study reports an easy method of a veterinary drug investigation in raw milk, based on QuECHERS extraction followed by RP-HPLC-UV analysis. Use of this benchtop system was motivated by its availability and moderate cost relatively to other sophisticated methods such as LC-MS which are more efficient. Methods: This developed method has been optimized and then after validation according to EU legislation, it demonstrated good linearity with R²>0.997, acceptable peak resolution within a short time (<9.5 min) and good recovery of the analyzed drugs (OXY, ALZ and IVR, respectively 87.08, 99.02 and 92.01 %). Additionally, we applied the method to the analysis of cow milk, collected in Nouakchott, capital of Mauritania. Results: The obtained results indicated a mixed level of drug use according to targeted molecules. In 42% of sampled farms, the anti-parasitics IVR and ALZ were detected whereas the antibiotic OXY was detected in 50%. Conclusion: This investigation shows that 17% of the sampled farms exceeded European standards for IVR drug.

Vital Tooth Whitening Techniques. A literature Review

2020 ◽  
Vol 02 ◽  
Author(s):  
Pia Chatterjee Kirk
Keyword(s):  
Soft Tissues ◽  
Treatment Time ◽  
Tooth Bleaching ◽  
Tooth Whitening ◽  
Easy Method ◽  
Tooth Structure ◽  
Dental Esthetics ◽  
Total Treatment Time ◽  
Restorative Materials ◽  
Total Treatment

Background: Vital tooth whitening has become an integral part of esthetic dentistry and remains one of the safest and most economic options today to improve dental esthetics without removing tooth structure. The tooth whitening materials have evolved into three categories: dentist-prescribed/dispensed (in office and patient home-use), and over-thecounter purchased and applied by patients. Objective: This review outlines the latest advances in dentist prescribed vital teeth whitening techniques, effects on tooth structure, soft tissues, and dental restoratives. Areas requiring additional research are also discussed. Methods: Electronic and manual literature search was conducted for key words such as tooth bleaching, and dental bleaching techniques using PubMed/MEDLINE, followed by manual selection of the studies that included whitening procedures in vital teeth. Results: The two main whitening agents are carbamide peroxide (CP) and hydrogen peroxide (HP or H2O2) whose concentration, duration of contact, and total treatment time can alter results. In addition, factors including the type of stain, and age of patient can affect results. Although whitening agents can affect tooth structure, restorative materials, and gingival tissues, the changes are temporary or can be treated using minimally invasive techniques. Conclusion: Areas requiring further research include the actual mechanism of whitening, its effect on tooth structure and restorative materials, and the development of an easy method to quantitate the degree of whitening in the dental office.

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