Effects of Polyvinyl Pyrrolidone and Activated Charcoal to Control Effect of Phenolic Oxidation on In Vitro Culture Establishment Stage of Micropropagation of Sugarcane (Saccharum Officinarum L.)

2015 ◽  
Vol 03 (04) ◽  
Author(s):  
Dereje Shimelis Kassahun Bantte ◽  
Tilaye Feyissa
Keyword(s):  
In Vitro Culture ◽  
Activated Charcoal ◽  
Saccharum Officinarum ◽  
Polyvinyl Pyrrolidone ◽  
Control Effect ◽  
Phenolic Oxidation

scholarly journals Biochar successfully replaces activated charcoal for in vitro culture of two white poplar clones reducing ethylene concentration

2012 ◽  
Vol 69 (1) ◽  
pp. 43-50 ◽  
Author(s):  
Sara Di Lonardo ◽  
Francesco Primo Vaccari ◽  
Silvia Baronti ◽  
Maurizio Capuana ◽  
Laura Bacci ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Activated Charcoal ◽  
White Poplar ◽  
Ethylene Concentration ◽  
Poplar Clones

scholarly journals Solidifying agents and activated charcoal for in vitro culture of Solanum sessiliflorum

2017 ◽  
Vol 52 (11) ◽  
pp. 1123-1126 ◽  
Author(s):  
Filipe Almendagna Rodrigues ◽  
Renata Alves Lara Silva Rezende ◽  
Moacir Pasqual ◽  
Maria Teresa Gomes Lopes
Keyword(s):  
In Vitro Culture ◽  
Activated Charcoal ◽  
Root Length ◽  
Culture Medium ◽  
In Vitro Cultivation ◽  
Fresh Matter ◽  
Number Of Leaves

Abstract: The objective of this work was to evaluate the effects of the solidifying agents agar and phytagel and of activated charcoal on the in vitro cultivation of two maná cubiu (Solanum sessiliflorum) varieties: Thaís and Santa Luzia. The phytotechnical characteristics analyzed included number of leaves, number of roots, shoot and root length, and fresh matter of shoot and root. Regardless of the variety, phytagel was superior to agar as a culture medium. A greater number of leaves and longer shoots were observed in the Santa Luzia variety, in the absence of charcoal. The Thaís variety showed longer shoots and roots in the presence of charcoal.

scholarly journals Protocol for development of zygotic embryos in vitro and acclimatization of Dragon’s Blood plantlets

2020 ◽  
Vol 9 (9) ◽  
pp. e231997031
Author(s):  
João Bosco de Oliveira Júnior ◽  
Camilla Mendes Pedroza Pessoa ◽  
Hendril da Silva Lopes ◽  
Frederico Henrique da Silva Costa ◽  
Jonny Everson Scherwinski Pereira
Keyword(s):  
In Vitro Culture ◽  
Plant Height ◽  
Activated Charcoal ◽  
Native Species ◽  
Culture Media ◽  
Medium Composition ◽  
Zygotic Embryos ◽  
Dragon’S Blood ◽  
In Vitro Establishment

Dragon's blood, native species from South America, has therapeutic properties scientifically proved. This study had the objective of developing a protocol for in vitro establishment and plantlets acclimatization from zygotic embryos, since researches with species are incipient. Culture media (MS, WPM, QL and N6) were assessed, without and with activated charcoal (2 g L-1). It was observed the percentage of developed embryos from 83% to 100% and the effect of medium composition for plant height and leaf number in vitro. The plantlets were acclimatized with 100% of survival rate, and for treatments from in vitro culture only the culture medium influenced diameter and plant height. The WPM and QL formulations without activated charcoal are indicated for in vitro culture of embryos and acclimatization of Dragon blood. The suggested methodology represents an important strategy for reproduction, physiological study and preservation of species.

scholarly journals Improvement of sugarcane (Saccharum officinarum L. var. Isd. 39) using gamma irradiation and large scale plantlet production from M1 generation through in vitro culture

2016 ◽  
Vol 5 (1) ◽  
pp. 1-9
Author(s):  
Shamima Akter ◽  
N Alam ◽  
PK Roy
Keyword(s):  
Gamma Radiation ◽  
In Vitro Culture ◽  
Large Scale ◽  
Saccharum Officinarum ◽  
Plantlet Production ◽  
In Vitro Technique ◽  
Half Strength ◽  
Leaf Segments ◽  
Agronomical Traits

The present study was aimed to develop mutant sugarcane (Saccharum officinarum L. var. Isd. 39) using gamma radiation followed by regeneration of those mutants through in vitro technique. Five different doses of gamma radiation (10, 20, 30, 40 and 50 Gy) were applied to the sugarcane setts and among these doses 10 Gy irradiated mutant sugarcane showed the best agronomical traits. In addition, 10 Gy irradiated mutant sugarcane also contained higher sugar concentrations (14.25%) compared to control (14.23%). On the other hand, shoot tip and young folded leaf segments from the 10 Gy irradiated plants (M1 generation) were used as explants in in vitro culture. It was evident from the experiment that direct shoot regeneration was better than indirect regeneration. Young leaf segments produced the highest percentage (84%) of shoots when cultured on MS+1.0 mg/l Kin + 0.2 mg/l NAA. The mean number of shoots was recorded as 35 ± 1.12 in the same medium. Moreover, addition of 4% sucrose and 10% coconut water (CW) in the same medium increased the number of shoot up to 45 per culture. Best rooting was obtained from shoots cultured on half-strength of MS fortified with 1.0mg/l NAA and 0.5mg/l IBA. Well-rooted plantlets were transferred to poly bag containing soil and compost (2:1) for hardening. During hardening 70% plantlets survived, which were subsequently transferred to the experimental field.Jahangirnagar University J. Biol. Sci. 5(1): 1-9, 2016 (June)

scholarly journals Regenerasi dan Pertumbuhan Beberapa Varietas Tebu (Saccharum officinarum L.) secara In Vitro

2011 ◽  
Vol 7 (2) ◽  
pp. 106 ◽  
Author(s):  
Deden Sukmadjaja ◽  
Ade Mulyana
Keyword(s):  
Tissue Culture ◽  
In Vitro Culture ◽  
Callus Induction ◽  
Callus Formation ◽  
Saccharum Officinarum ◽  
Sugarcane Varieties ◽  
Media Formulation ◽  
Research Activities

<p>(Saccharum officinarum L.) through In Vitro Culture.<br />Deden Sukmadjaja and Ade Mulyana. The research was<br />conducted at the Laboratory of Tissue Culture The Biology of<br />Cell and Tissue Researcher Group ICABIOGRAD, Bogor from<br />June to November 2009 to studied growth and regenerations<br />response some varieties of sugarcane through in vitro<br />culture. The research activities have been carried out in<br />three steps, i.e., callus formation, regeneration of shoots and<br />roots regeneration. The type of explants used in the study<br />was in vitro planlet explants of both sugarcane varieties.<br />Seven media formulations were used for the callus induction<br />and regeneration of shoots, while five media formulations<br />were used for the roots regeneration. The results<br />showed that the highest respond for calluses induction was<br />Bulu Lawang varieties at media formulation MS + 2.4-D 2<br />mg.l-1 + BAP 0.4 mg.l-1 + CH 2000 mg.l-1 and PS 951 varieties<br />at media formulation MS + 2.4-D 1 mg.l-1 + BAP 0.4 mg.l-1.<br />While the highest respond for regeneration of shoots was<br />Bulu Lawang varieties at media formulation MS0 (control<br />MS) dan PS 951 varieties at media formulation MS + BAP 1<br />mg.l-1 + kinetin 1 mg.l-1 + NAA 0.5 mg.l-1 + GA3 0.5 mg.l-1.<br />The highest respond of roots regeneration was Bulu Lawang<br />and PS 951 varieties at media formulation MS + IBA 1 mg.l-1.<br />Acclimatization of plantlets produced were grew successfully<br />about 90-100% in greenhouse.</p>

scholarly journals Clonal Propagation of Phalaenopsis amabilis (L.) BL. cv. 'Golden Horizon' Through In vitro Culture of Leaf Segments

1970 ◽  
Vol 46 (2) ◽  
pp. 163-168 ◽  
Author(s):  
P Sinha ◽  
MAA Jahan
Keyword(s):  
In Vitro Culture ◽  
Natural Environment ◽  
Activated Charcoal ◽  
Clonal Propagation ◽  
Ms Medium ◽  
Huge Amount ◽  
Leafy Shoots ◽  
Half Strength ◽  
Leaf Segments

A protocol was established for mass clonal propagation of Phalaenopsis amabilis cv. 'Golden horizon' through in vitro culture of young leaf segments from mature plant. Explants were cultured on half strength Murashige and Skoog (1/2 MS) medium supplemented with N6-benzyladenine (2.0 mg l-1), a-naphthaleneaceetic acid (0.5 mg l-1), 2% (w/v) sucrose, 10% (v/v) coconut water, 2 g l-1 peptone and 1 g l-1 activated charcoal. Each section of explant produced 15 protocorm-like bodies (PLBs) after 12 weeks of culture. When phytohormone was omitted from the medium and 150 mg l-1 L-glutamone was added PLBs were found to be enlarged with leafy shoots and new PLBs were induced from the base of the old ones. Leafy shoots rooted on half strength MS medium supplemented with 2 g l-1 peptone, 2% (w/v) sucrose, 10% (v/v) CW and 1 g l-1 activated charcoal, where 100% explants were developed into plantlets with roots within 8 weeks. The addition of 2.5 g l-1 banana pulp powder enhanced the number and length of roots. Within the first 32 weeks after initiation of culture about 1500 plantlets as well as a huge amount of PLBs were achieved from a single explant section. The plantlets were acclimatized in natural environment. Key words: Phalaenopsis orchid; Leaf segments; Protocorm-like bodies; Micropropagation DOI: http://dx.doi.org/10.3329/bjsir.v46i2.8182 Bangladesh J. Sci. Ind. Res. 46(2), 163-168, 2011

scholarly journals Pengaruh Myoinositol dan Arang Aktif terhadap Pertumbuhan Planlet Anggrek Dendrobium dalam Kultur In Vitro

2013 ◽  
Vol 22 (3) ◽  
pp. 205 ◽  
Author(s):  
Dyah Widiastoeti ◽  
Anggraeni Santi ◽  
Nina Solvia
Keyword(s):  
Root Growth ◽  
In Vitro Culture ◽  
Activated Charcoal ◽  
Critical Factor ◽  
Media Optimization ◽  
Randomized Design ◽  
Plantlet Growth ◽  
Ornamental Crops ◽  
Completely Randomized Design

ABSTRAK.  Anggrek Dendrobium merupakan tanaman hias komersial yang sangat penting di Indonesia. Optimasi media dalam kultur in vitro sangat diperlukan untuk meningkatkan dan mempercepat pertumbuhan planlet. Salah satu cara untuk mengoptimalisasi media in vitro yaitu dengan pemberian myoinositol dan arang aktif. Tujuan penelitian ialah mengetahui pengaruh myoinositol dan arang aktif terhadap pertumbuhan planlet Dendrobium. Penelitian dilakukan di Laboratorium Kultur Jaringan, Balai Penelitian Tanaman Hias Pasarminggu, Jakarta mulai Bulan Juni sampai dengan Desember 2010.  Metode penelitian menggunakan rancangan acak  kelompok dengan delapan perlakuan dan lima ulangan. Perlakuan terdiri atas konsentrasi myoinositol  0, 50, 100, dan 150 mg/l dengan dan tanpa penambahan arang aktif. Hasil penelitian menunjukkan bahwa pemberian myoinositol 50 mg/l tanpa arang aktif dapat meningkatkan tinggi planlet, panjang dan lebar daun, sedangkan myoinositol 100 mg/l dengan penambahan arang aktif 2 g/l meningkatkan pertumbuhan jumlah dan panjang akar terbaik.<br /><br />ABSTRACT. Widiastoety, D, Santi, A, and Solvia, N 2012. Effect of Myoinositol and Activated Charcoal on the Growth of Dendrobium Orchid Plantlets in In Vitro Culture. This study was aims to determine the effect of myoinositol and activated charcoal on the growth of Dendrobium plantlets. Dendrobium is one of the most important commercial orchids in Indonesia. Media optimization is critical factor to improve and to promote plantlet growth.  One of the methods to enrich the medium was by the use of myoinositol and activated charcoal. The study was conducted at the In Vitro Culture Laboratory of Indonesian Ornamental Crops Plants Research Institute Pasarminggu, Jakarta from June through December 2010. A completely randomized design with eight treatments and five replications was used in this experiment. The treatments given were myoinositol 0, 50, 100, and 150 mg/l with and without addition of activated charcoal. The results showed that application of myoinositol 50 mg/l without activated charcoal enhance the plantlet height, length, and leave width, while myoinositol 100 mg/l with addition of activated charcoal 2 g/l increased the highest number of the root and the longest root growth.<br /><br />

scholarly journals Influence of activated charcoal on seed germination and seedling development by the asymbiotic method in Zygostates grandiflora (Lindl.) Mansf. (Orchidaceae)

2010 ◽  
Vol 22 (2) ◽  
pp. 45-50 ◽  
Author(s):  
Agata Pacek-Bieniek ◽  
Magdalena Dyduch-Siemińska ◽  
Michał Rudaś
Keyword(s):  
Seed Germination ◽  
In Vitro Culture ◽  
Activated Charcoal ◽  
Indoleacetic Acid ◽  
Positive Influence ◽  
Seedling Development ◽  
Aerial Roots ◽  
Murashige And Skoog Medium ◽  
Intensive Development

Abstract The aim of this study is to examine the influence of activated charcoal on the seed germinating ability and seedling development of Zygostates grandiflora (Lindl.) Mansf. in in vitro culture. The seeds were obtained from a sterilised orchid capsule. They were sown on a Murashige and Skoog medium + indoleacetic acid (1.5 mg dm-3) and benzylaminopurine (2.0 mg dm-3) without the addition of activated charcoal and on a medium that contained 1.0 and 3.0 g dm-3 activated charcoal, respectively, for 18 months. Initial differences in seed germination were observed after nine months since the beginning of the in vitro culture. The addition of activated charcoal had a positive influence on protocorm size and development. The results of this research were confirmed after 12 and 18 months of in vitro culture, where an intensive development of leaf and aerial roots occurred on a medium that contained the highest concentration of activated charcoal, i.e. 3.0 g dm-3 as a supplement. The statistical analysis showed that the asymbiotic method of orchid propagation in in vitro culture could be used for Z. grandiflora, and that the addition of activated charcoal into the medium improved this method.

Isohexenylnaphthazarins from Lithospermum canescens (Michx.) Lehm. and Arnebia euchroma (Royle) Jonst. in vitro culture

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
K Graikou ◽  
H Damianakos ◽  
K Syklowska-Baranek ◽  
A Pietrosiuk ◽  
M Jeziorek ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Arnebia Euchroma ◽  
Lithospermum Canescens
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