scholarly journals Evaluation for High Iron and Zinc Content among Selected Climbing Bean Genotypes in Rwanda

2018 ◽  
Vol 06 (02) ◽  
Author(s):  
Mukamuhirwa F ◽  
Rurangwa E
Keyword(s):  
Author(s):  
Dhiraj Gangtire ◽  
Nakul D. Magar ◽  
Vaibhav Khelurkar ◽  
Mangesh P. Moharil ◽  
P. V. Jadhav ◽  
...  

Foxtail millet (Setaria italica L.) is nutri-cereal crop having it is rich in β-carotene, vitamin B-complex and micronutrients like minerals. In the present research, we have studied biochemical properties and molecular profiling to identify the core set of foxtail millet (Setaria italic L.) accessions for high Iron (Fe) and Zinc (Zn) content. Total seventy-nine accessions and selected mutants variety PS4 of foxtail millet were used. The biochemical investigation revealed that accessions M2-106, IC120407, M3-61/HB-13, and IC120255 consist of high iron and zinc content. The genetic variability among the genotypes was revealed by 28 Promoter Anchored Amplified Polymorphic (PAAP-RAPD) primers of which OPE9+GC1, OPE9+CA1, UBC001+CA1, UBC001+TA1, UBC693+G1 showed 100% polymorphism, whereas UBC693+GC1 and OPE7+G1 showed 88% and 80% polymorphism, respectively, with an average of 45.95 % polymorphism. Total alleles per locus were 3.31, whereas, the average number of monomorphic and polymorphic alleles were 1.72 and 1.56, respectively. The extent of polymorphic information content (PIC) of PAAP-RAPD loci ranged from 0.5 to 0.87 with an average value of 0.41. For PAAP-RAPD, the maximum PIC value was observed in marker UBC693+GC1 (0.87 %) and the minimum were OPE5+CA1 and OPE9+CA1 (0.5 %). Molecular characterizations result showing highest similarity (0.932) between accessions Shrilakshmi and Prasad, whereas, the lowest similarity coefficient was observed between IC120255 and M3-75/AM-1 (0.697) with PS4.UPGMA dendrogram grouped the foxtail millet accessions in five clusters which marked high diversity in M3-61/HB-13 and M3-75/AM-1. It implies that PAAP-RAPD markers are significantly screened in the foxtail millet accessions and have enumerated high genetic diversity.


2017 ◽  
Vol 40 (04) ◽  
Author(s):  
Vijayata Singh ◽  
R. K. Yadav ◽  
N. R. Yadav ◽  
Rajesh Yadav ◽  
R. S. Malik ◽  
...  

Among the legumes, mungbean has highest digestive protein but low micronutrient content like iron and zinc. Biofortification of mungbean has been undertaken to reduce micronutrient malnutrition. The objectives of this study were to identify QTLs for seed Fe and Zn content in F6 recombinant inbred line (RIL) population (ML776 x Sattya). A large genetic variation and transgressive segregation in RILs were observed for Fe and Zn content. Linkage map was developed which spanned 2919.7cM distance. 17 QTLs (2 for iron and 15 for zinc content) were mapped on four linkage groups; LG 4, LG 6, LG 7 and LG 11 in mungbean. The genomic regions qZn-4-3 and qFe-4-1 on chromosome 4 between PVBR82-BM210 markers; qZn-11-2 and qFe-11-1 on chromosome11 between BM141-BM184 markers, were co-located on the same chromosomal regions for Zn or Fe concentration, which probably were closely linked to each other, or were the same pleiotropic QTLs. The SSR markers associated with QTLs for both high iron and zinc content would also be useful in marker assisted breeding for biofortification in mungbean.


2018 ◽  
Vol 50 (4) ◽  
pp. 472-477
Author(s):  
Eung-Gi Jeong ◽  
Eok-Keun Ahn ◽  
Yong-Jae Won ◽  
Jeong-Heui Lee ◽  
Sang-Bok Lee ◽  
...  

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Shilpi Dixit ◽  
Uma Maheshwar Singh ◽  
Ragavendran Abbai ◽  
T. Ram ◽  
Vikas Kumar Singh ◽  
...  

Bragantia ◽  
2012 ◽  
Vol 71 (3) ◽  
pp. 336-341 ◽  
Author(s):  
Camila Andrade Silva ◽  
Ângela de Fátima Barbosa Abreu ◽  
Magno Antonio Patto Ramalho ◽  
Angelita Duarte Corrêa

The mineral contents in common bean seeds are influenced, in addition to genetic variation, by environmental crop conditions, especially by the soil type and chemical composition and by the genotype x environment interaction. This study was carried out to verify if the zinc and iron contents are affected by the crop growing period. Ten lines with high iron and zinc contents and ten with low contents were assessed in three seasons: "wet season" of 2009/2010 (sowing in November); "dry season" of 2010 (sowing in February) and "winter season" of 2010 (sowing in July), in Lavras, Minas Gerais State, Brazil. The experimental design used was randomized blocks with three replications and plots consisting of two rows of two meters, with a spacing of 0.50 m. The seeds harvested were assessed in regard to iron and zinc mineral contents. The greatest contents were observed in the winter season and the smallest ones in the dry season, with sowing in February. It was observed that in the mean of the three harvests, the lines classified as having high iron and zinc content exhibited an iron quantity 11.0% and a zinc quantity 6.8% above those of low content. The lines by seasons interaction occurs. However, its interference in identification of the groups with high and low content of the two nutrients is not great.


2020 ◽  
Vol 100 (11) ◽  
pp. 4150-4164 ◽  
Author(s):  
Shilpa M Naik ◽  
Anitha K Raman ◽  
Minnuru Nagamallika ◽  
Challa Venkateshwarlu ◽  
Suresh Prasad Singh ◽  
...  
Keyword(s):  

2017 ◽  
Vol 9 (12) ◽  
pp. 241 ◽  
Author(s):  
Casinga Mubasi Clérisse ◽  
Neema Ciza Angélique ◽  
Kajibwami Cikuru Marie-Angélique ◽  
Nabahungu Nshwarasi Leon ◽  
Mambani Banda Pierre

This study investigated the influence of three soil moisture irrigation regimes on concentration of seed iron and zinc content of four biofortified bean varieties promoted for eradication of malnutrition in Sud-Kivu highlands. A field experiment was conducted in the Hogola marsh highlands during two cultural seasons B2013 and B2014. The experiment design was a RCBD with a split plot arrangement where the main plots were 110 m2 and split plots 20 m2. A strategic application of homogenisation of the experimental site’s soil fertility by chemical fertilizers of the type: CaCO3, KCl and DAP was conducted out. Four biofortified varieties (CODMLB001, RWR2245, HM21-7 and RWK10) constituted main factor, while water regimes respectively [bottom of the slope: R1 = 48% soil moisture, at the middle of the slope R2 = 37% soil moisture and at the top of the slope: R3 = 29% soil moisture according to the gradient of humidity] represented secondary factor and seasonality, tertiary factor. The study showed that the concentrations of iron and zinc were highly correlated with soil moisture regimes. The variety HM21-7 demonstrated better adaptability because it showed a low rate of reduction of iron and zinc concentration under the three soil moisture regimes and was therefore best suited to fight malnutrition in the Sud-Kivu province.


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