scholarly journals Antimicrobial Resistance Patterns and Molecular Characterization of <i>Klebsiella pneumoniae</i> in Clinical Isolates at Mbarara Regional Referral Hospital

2019 ◽  
Vol 09 (03) ◽  
pp. 197-225
Author(s):  
Joseph Turugurwa ◽  
James Mwesigye ◽  
Kennedy Kassaza ◽  
Fredrick Byarugaba ◽  
Taseera Kabanda ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Klebsiella Pneumoniae ◽  
Molecular Characterization ◽  
Referral Hospital ◽  
Clinical Isolates ◽  
Regional Referral Hospital ◽  
Resistance Patterns

scholarly journals Susceptibility ofKlebsiella pneumoniaeIsolates from Intra-Abdominal Infections and Molecular Characterization of Ertapenem-Resistant Isolates

2011 ◽  
Vol 55 (8) ◽  
pp. 3917-3921 ◽  
Author(s):  
Stephen P. Hawser ◽  
Samuel K. Bouchillon ◽  
Christine Lascols ◽  
Meredith Hackel ◽  
Daryl J. Hoban ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Klebsiella Pneumoniae ◽  
Molecular Characterization ◽  
Clinical Isolates ◽  
Extended Spectrum ◽  
Clinical Breakpoints ◽  
Abdominal Infections

ABSTRACTA total of 2,841 clinical isolates ofKlebsiella pneumoniaefrom intra-abdominal infections worldwide were collected in the Study for Monitoring Antimicrobial Resistance Trends (SMART) during 2008 and 2009. Overall, 22.4% of isolates had extended-spectrum β-lactamases (ESBLs). The most active antibiotics among the 11 tested were imipenem, amikacin, and ertapenem, though even these, like all other comparators, were less consistently active against ESBL-positive isolates than against ESBL-negative isolates. Globally, 6.5% of isolates were ertapenem resistant based on the June 2010 clinical breakpoints published by the Clinical and Laboratory Standards Institute, with MICs of ≥1 μg/ml. Molecular characterization of 43 isolates with ertapenem MICs of ≥4 μg/ml showed that they variously produced CTX-M or SHV ESBLs combined with altered impermeability and/or had KPC (n= 28), OXA-48 (n= 3), or VIM (n= 1) carbapenemases. Further monitoring of ertapenem susceptibility and molecular characterization of ertapenem-resistant isolates are needed.

scholarly journals Molecular characterization of multidrug resistant Klebsiella pneumoniae clinical isolates recovered from King Abdulaziz Specialist Hospital at Taif City, Saudi Arabia

2021 ◽  
Vol 14 (1) ◽  
pp. 143-151
Author(s):  
Rihab Lagha ◽  
Fethi Ben Abdallah ◽  
Asmaa A.H. ALKhammash ◽  
Nabil Amor ◽  
Mohamed M. Hassan ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Klebsiella Pneumoniae ◽  
Molecular Characterization ◽  
Multidrug Resistant ◽  
Clinical Isolates

Molecular characterization of antimicrobial resistance in Klebsiella pneumoniae isolated from Brazilian dairy herds

2021 ◽  
Author(s):  
Diego Borin Nobrega ◽  
Aline Parolin Calarga ◽  
Leandro Costa Nascimento ◽  
Carla Gasparotto Chande Vasconcelos ◽  
Eliane Martins de Lima ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Klebsiella Pneumoniae ◽  
Molecular Characterization ◽  
Dairy Herds

scholarly journals Prevalence and molecular characterization of antibiotic resistance and associated genes in Klebsiella pneumoniae isolates: A clinical observational study in different hospitals in Chattogram, Bangladesh

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0257419
Author(s):  
Afroza Akter Tanni ◽  
Md. Mahbub Hasan ◽  
Nahid Sultana ◽  
Wazir Ahmed ◽  
Adnan Mannan
Keyword(s):  
Multidrug Resistance ◽  
Klebsiella Pneumoniae ◽  
Molecular Characterization ◽  
Clinical Isolates ◽  
Diffusion Method ◽  
Multi Drug Resistance ◽  
Pcr Analysis ◽  
Resistant Bacteria ◽  
Disk Diffusion Method

Objective This study was performed to investigate the prevalence of multidrug resistance and molecular characterization of Klebsiella pneumoniae (KPN) from clinical isolates in the southern region of Bangladesh. Additional analysis of the prevalence of blaNDM-1, blaSHV-11, uge genes of KPN was also carried out among these clinical isolates. Method The study was carried out using 1000 clinical isolates collected from two different hospitals of Chattogram. A drug susceptibility test was performed by the disk diffusion method to detect KPN’s response to 16 antibiotics. The presence of antibiotic-resistant and (or) virulent genes blaNDM-1, blaSHV-11, uge were investigated using the PCR technique. Isolates having blaNDM-1, blaSHV-11, uge gene were further validated by sequencing followed by phylogenetic analysis. Phylogenetic relationships among these isolates were determined by Clustal omega and MEGA7. Result A total of 79%, 77%, 74.9%, 71%, 66% and 65% isolates exhibited resistance against cefuroxime, cefixime, cefotaxime, ceftazidime, cefepime and ceftriaxone respectively. The frequency of resistance to other antibiotics varied from 26.5% to 61.8%. PCR analysis showed that 64% of strains harbored blaNDM-1 gene, and 38% strains harbored blaSHV-11 gene. Moreover, 47% of samples were carrying uge gene, and 19% of samples carried blaNDM-1, blaSHV-11, uge genes together. Conclusion In this study, we’ve analysed the pattern of expression as well as prevalence of blaNDM-1, blaSHV-11, and uge genes in Klebsiella isolates. Upon molecular and statistical analysis, we found a high prevalence of multi-drug resistance KPN strains in the isolates. The Klebsiella isolates were confirmed to harbor multiple ESBL genes and 64% of the isolates were found to be producing NDM-1. As multidrug resistance is an alarming issue, continuous surveillance and routine clinical detection of resistant bacteria and plasmids are necessary to prevent catastrophic public health incidents.

Occurrence and molecular characterization of Klebsiella pneumoniae ST37 clinical isolates producing plasmid-mediated AmpC recovered over a 3-year period

2012 ◽  
Vol 74 (1) ◽  
pp. 95-97 ◽  
Author(s):  
Marina Illiaquer ◽  
Nathalie Caroff ◽  
Pascale Bémer ◽  
Guillaume G. Aubin ◽  
Marie-Emmanuelle Juvin ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Molecular Characterization ◽  
Clinical Isolates
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