scholarly journals Green Synthesis of Silver Nanoparticles Using Cannabis sativa Extracts and Their Anti-Bacterial Activity

2021 ◽  
Vol 11 (01) ◽  
pp. 28-38
Author(s):  
Sujata Mandal ◽  
Sreekar B. Marpu ◽  
Roxana Hughes ◽  
Mohammad A. Omary ◽  
Sheldon Q. Shi
Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4041
Author(s):  
Adriana Cecilia Csakvari ◽  
Cristian Moisa ◽  
Dana G. Radu ◽  
Leonard M. Olariu ◽  
Andreea I. Lupitu ◽  
...  

Cannabis sativa L. (hemp) is a plant used in the textile industry and green building material industry, as well as for the phytoremediation of soil, medical treatments, and supplementary food products. The synergistic effect of terpenes, flavonoids, and cannabinoids in hemp extracts may mediate the biogenic synthesis of metal nanoparticles. In this study, the chemical composition of aqueous leaf extracts of three varieties of Romanian hemp (two monoecious, and one dioecious) have been determined by Fourier-Transformed Infrared spectroscopy (FT-IR), high-performance liquid chromatography, and mass spectrometry (UHPLC-DAD-MS). Then, their capability to mediate the green synthesis of silver nanoparticles (AgNPs) and their pottential antibacterial applications were evaluated. The average antioxidant capacity of the extracts had 18.4 ± 3.9% inhibition determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 78.2 ± 4.1% determined by 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS™) assays. The total polyphenolic content of the extracts was 1642 ± 32 mg gallic acid equivalent (GAE) L−1. After this, these extracts were reacted with an aqueous solution of AgNO3 resulting in AgNPs, which were characterized by UV−VIS spectroscopy, FT-IR, scanning electron microscopy (SEM-EDX), and dynamic light scattering (DLS). The results demonstrated obtaining spherical, stable AgNPs with a diameter of less than 69 nm and an absorbance peak at 435 nm. The mixture of extracts and AgNPs showed a superior antioxidant capacity of 2.3 ± 0.4% inhibition determined by the DPPH• assay, 88.5 ± 0.9% inhibition as determined by the ABTS•+ assay, and a good antibacterial activity against several human pathogens: Escherichia coli, Klebsiella pneumoniae, Pseudomonas fluorescens, and Staphylococcus aureus.


2014 ◽  
Vol 9 (1) ◽  
Author(s):  
Pornanong Aramwit ◽  
Nipaporn Bang ◽  
Juthamas Ratanavaraporn ◽  
Sanong Ekgasit

2020 ◽  
Vol 24 ◽  
pp. 100848
Author(s):  
Giriraj Tailor ◽  
B.L. Yadav ◽  
Jyoti Chaudhary ◽  
Manoj Joshi ◽  
Chetana Suvalka

2014 ◽  
Vol 9 (1) ◽  
Author(s):  
Pornanong Aramwit ◽  
Nipaporn Bang ◽  
Juthamas Ratanavaraporn ◽  
Thanasorn Thanavibul ◽  
Sanong Ekgasit

2017 ◽  
Vol 3 (4) ◽  
pp. 1-6 ◽  
Author(s):  
Subramani Srinivasan ◽  
Kathiroli Sujithra ◽  
Udaiyar Muruganathan ◽  
Raju Murali ◽  
Chandran Navetha ◽  
...  

2018 ◽  
Vol Volume 13 ◽  
pp. 3571-3591 ◽  
Author(s):  
Priyanka Singh ◽  
Santosh Pandit ◽  
Jørgen Garnæs ◽  
Sanja Tunjic ◽  
Venkata Mokkapati ◽  
...  

2021 ◽  
pp. 116422
Author(s):  
Kakali Baruah ◽  
Mahabul Haque ◽  
Leader Langbang ◽  
Sourav Das ◽  
Kripamoy Aguan ◽  
...  

2020 ◽  
Vol 21 (3) ◽  
pp. 206-218 ◽  
Author(s):  
Sadia Nazer ◽  
Saiqa Andleeb ◽  
Shaukat Ali ◽  
Nazia Gulzar ◽  
Tariq Iqbal ◽  
...  

Background: Multi-drug resistance in bacterial pathogens is a major concern of today. Green synthesis technology is being used to cure infectious diseases. Objectives: The aim of the current research was to analyze the antibacterial, antioxidant, and phytochemical screening of green synthesized silver nanoparticles using Ajuga bracteosa. Methods: Extract of A. bracteosa was prepared by maceration technique. Silver nanoparticles were synthesized using A. bracteosa extract and were confirmed by UV-Vis spectrophotometer, Scanning Electron Microscope (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). The antibacterial, anti-biofilm, cell proliferation inhibition, TLC-Bioautography, TLC-Spot screening, antioxidant, and phytochemical screening were also investigated. Results: UV-Vis spectrum and Scanning electron microscopy confirmed the synthesis of green nanoparticles at 400 nm with tube-like structures. FTIR spectrum showed that functional groups of nanoparticles have a role in capping and stability of AgNP. Agar well diffusion assay represented the maximum antibacterial effect of ABAgNPs against Escherichia coli, Klebsiella pneumoniae, Streptococcus pyogenes, Staphylococcus aureus, and Pseudomonas aeruginosa at 0.10 g/mL concentration compared to ABaqu. Two types of interactions among nanoparticles, aqueous extract, and antibiotics (Synergistic and additive) were recorded against tested pathogens. Crystal violet, MTT, TLC-bio-autography, and spot screening supported the findings of the antibacterial assay. Highest antioxidant potential effect in ABaqu was 14.62% (DPPH) and 13.64% (ABTS) while 4.85% (DPPH) and 4.86% (ABTS) was recorded in ABAgNPs. Presence of phytochemical constituents showed pharmacological importance. Conclusion: It was concluded that green synthesis is an innovative technology in which natural products are conjugated with metallic particles and are used against infectious pathogens. The current research showed the significant use of green nanoparticles against etiological agents.


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