Genetic polymorphisms in the carbonic anhydrase VI gene and dental caries susceptibility

This study investigated pH, activity and concentration of carbonic anhydrase VI (CA VI) in dental biofilm of caries and caries-free children of 7–9 years old. Seventy-four children were selected and divided into two groups. The caries diagnosis was performed according to the WHO criteria, including the early caries lesion. After biofilm collection and pH determination, CA VI concentration and activity were determined by ELISA and Zimography respectively. The data were submitted to a Mann-Whitney test and to Pearson and Spearman correlation analyses. Means and standard deviations of dental caries for the caries group were of 3.162 ± 1.385. The biofilm pH was significantly higher in the caries-free group. The CA VI activity was significantly higher in biofilm of children with caries. The CA VI concentration was significantly higher in biofilm of caries-free children. In caries-free children, there was a moderate negative correlation between CA VI activity and concentration in dental biofilm as well as between pH and CA VI activity. A negative correlation between biofilm pH and CA VI concentration was found in the caries group. In conclusion, CA VI was shown to be more active in the biofilm of school children with caries in order to contribute to neutralization of biofilm acid.

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Effects of the carbonic anhydrase VI gene polymorphisms on dental caries: A meta-analysis

Dental and Medical Problems ◽

10.17219/dmp/110453 ◽

2019 ◽

Vol 56 (4) ◽

pp. 395-400

Author(s):

Omer Hatipoglu ◽

Faruk Saydam

Keyword(s):

Dental Caries ◽

Carbonic Anhydrase ◽

Gene Polymorphisms ◽

Meta Analysis ◽

Carbonic Anhydrase Vi

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Effect of Genetic Polymorphisms in CA6 Gene on the Expression and Catalytic Activity of Human Salivary Carbonic Anhydrase VI

Caries Research ◽

10.1159/000350414 ◽

2013 ◽

Vol 47 (5) ◽

pp. 414-420 ◽

Cited By ~ 15

Author(s):

M. Aidar ◽

Rocha Marques ◽

J. Valjakka ◽

N. Mononen ◽

T. Lehtimäki ◽

...

Keyword(s):

Catalytic Activity ◽

Carbonic Anhydrase ◽

Genetic Polymorphisms ◽

Carbonic Anhydrase Vi

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CHOP-Dependent Stress-Inducible Expression of a Novel Form of Carbonic Anhydrase VI

Molecular and Cellular Biology ◽

10.1128/mcb.19.1.495 ◽

1999 ◽

Vol 19 (1) ◽

pp. 495-504 ◽

Cited By ~ 102

Author(s):

John Sok ◽

Xiao-Zhong Wang ◽

Nikoleta Batchvarova ◽

Masahiko Kuroda ◽

Heather Harding ◽

...

Keyword(s):

Carbonic Anhydrase ◽

Target Gene ◽

Target Genes ◽

Direct Evidence ◽

Nuclear Protein ◽

Intracellular Form ◽

Carbonic Anhydrase Vi ◽

Responsive Element

ABSTRACT CHOP (also called GADD153) is a stress-inducible nuclear protein that dimerizes with members of the C/EBP family of transcription factors and was initially identified as an inhibitor of C/EBP binding to classic C/EBP target genes. Subsequent experiments suggested a role for CHOP-C/EBP heterodimers in positively regulating gene expression; however, direct evidence that this is the case has so far not been uncovered. Here we describe the identification of a positively regulated direct CHOP-C/EBP target gene, that encoding murine carbonic anhydrase VI (CA-VI). The stress-inducible form of the gene is expressed from an internal promoter and encodes a novel intracellular form of what is normally a secreted protein. Stress-induced expression of CA-VI is both CHOP and C/EBPβ dependent in that it does not occur in cells deficient in either gene. A CHOP-responsive element was mapped to the inducibleCA-VI promoter, and in vitro footprinting revealed binding of CHOP-C/EBP heterodimers to that site. Rescue of CA-VIexpression in c/ebpβ−/− cells by exogenous C/EBPβ and a shorter, normally inhibitory isoform of the protein known as LIP suggests that the role of the C/EBP partner is limited to targeting the CHOP-containing heterodimer to the response element and points to a preeminent role for CHOP in CA-VI induction during stress.

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An association analysis for genetic factors for dental caries susceptibility in a cohort of Chinese children

Oral Diseases ◽

10.1111/odi.13758 ◽

2020 ◽

Author(s):

Lingli Wu ◽

Zhiqiang Li ◽

Jianye Zhou ◽

Bin Ma ◽

Fei Yu ◽

...

Keyword(s):

Dental Caries ◽

Association Analysis ◽

Genetic Factors ◽

Chinese Children ◽

Dental Caries Susceptibility

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Association of β-defensin 1 gene Polymorphism and dental caries susceptibility in Tamil Ethnicity

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00823 ◽

2021 ◽

pp. 4731-4735

Author(s):

Harini Venkata Subbiah ◽

Usha Subbiah ◽

Athira Ajith

Keyword(s):

Dental Caries ◽

Odds Ratio ◽

Regulatory Elements ◽

Microbial Colonization ◽

Nucleotide Polymorphisms ◽

First Line ◽

Single Nucleotide ◽

Variant Genotype ◽

Dental Caries Susceptibility ◽

Genetic And Environmental Factors

Dental caries is a multifactorial disease that affects a large proportion of the population with both genetic and environmental factors contributing to the disease. Even in healthy oral environmental conditions, some individuals are susceptible to dental caries due to potential genetic contribution. Antimicrobial peptides are expressed in oral cavity and play an important role against microbial colonization and form an important first line defense against cariogenic bacteria. In the present study, we attempt to identify genetic variants that would cause significant functional impact towards susceptibility to dental caries. We investigated single nucleotide polymorphisms (SNPs) of beta-defensin 1 (DEFB1) as predictors of dental caries in tamil ethnic population. A total of 120 subjects were recruited for this study, which included 60 dental caries patients (DMFT>5) and 60 healthy controls (DMFT=0). Three SNPs of 5’UTR regulatory elements of DEFB1 were genotyped by PCR followed by Sanger sequencing. The genotypes associated with susceptibility to caries were found to be significant between rs11362 (p=.025, odds ratio = 3.72, 95% confidence interval (CI) = 1.289-10.742), rs1799946 (p=.023, odds ratio=4.32, 95% CI = 1.33-14.028) gene polymorphisms and risk of dental caries (DMFT>5) in tamil ethnicity. The variant genotype GG of rs1800972 polymorphism was found to be high in cases than controls but was not significant (p=0.136). Our data suggested that β-defensin 1 polymorphisms play a role in the susceptibility to dental caries.

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Association of ENAM, TUFT1, MMP13, IL1B, IL10 and IL1RN gene polymorphism and dental caries susceptibility in Chinese children

Journal of International Medical Research ◽

10.1177/0300060519828450 ◽

2019 ◽

Vol 47 (4) ◽

pp. 1696-1704 ◽

Cited By ~ 6

Author(s):

Xiao-Pan Hu ◽

Tian-Zhu Song ◽

Yan-Yan Zhu ◽

Ling-Li Wu ◽

Xuan Zhang ◽

...

Keyword(s):

Dental Caries ◽

Interleukin 1 ◽

Saliva Sample ◽

Northwest China ◽

Interleukin 10 ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Significant Difference ◽

Dental Caries Susceptibility ◽

Matrix Metallopeptidase

Objective To investigate the association between single nucleotide polymorphisms (SNPs) in six candidate genes (enamelin [ ENAM]; tuftelin 1 [ TUFT1]; matrix metallopeptidase 13 [ MMP13]; interleukin 1 beta [ IL1B]; interleukin 10 [ IL10]; interleukin 1 receptor antagonist [ IL1RN]) and dental caries in children from northwest China. Methods This case–control study enrolled children (12–15 years) who underwent routine dental examinations. The children were divided into two groups based on the presence of dental caries. A saliva sample was collected and seven SNPs (rs3806804A/G in ENAM, rs3811411T/G in TUFT1, rs2252070A/G and rs597315A/T in MMP13, rs1143627C/T in IL1B, rs1800872A/C in IL10 and rs956730G/A in IL1RN) were genotyped. Results A total of 357 children were enrolled in the study: 161 with dental caries and 196 without dental caries. No significant difference was found in the alleles and genotypes of five genes ( ENAM, TUFT1, MMP13, IL10 and IL1RN) between those with and without dental caries. A significant relationship was found between the IL1B rs1143627C/T polymorphism and dental caries susceptibility with those carrying the rs1143627CT genotype having a lower risk of dental caries compared with those carrying the CC genotype (odds ratio 0.557; 95% confidence interval 0.326, 0.952). Conclusion The IL1B rs1143627C/T polymorphism may be associated with dental caries susceptibility in children from northwest China.

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