scholarly journals Activity of Carbonic Anhydrase VI is Higher in Dental Biofilm of Children with Caries

2019 ◽  
Vol 20 (11) ◽  
pp. 2673 ◽  
Author(s):  
Daniele C. R. Picco ◽  
Lenita Marangoni-Lopes ◽  
Thaís M. Parisotto ◽  
Renata Mattos-Graner ◽  
Marinês Nobre-dos-Santos
Keyword(s):  
Dental Caries ◽  
Carbonic Anhydrase ◽  
Negative Correlation ◽  
School Children ◽  
Spearman Correlation ◽  
Who Criteria ◽  
Dental Biofilm ◽  
Moderate Negative Correlation ◽  
Carbonic Anhydrase Vi ◽  
Early Caries

This study investigated pH, activity and concentration of carbonic anhydrase VI (CA VI) in dental biofilm of caries and caries-free children of 7–9 years old. Seventy-four children were selected and divided into two groups. The caries diagnosis was performed according to the WHO criteria, including the early caries lesion. After biofilm collection and pH determination, CA VI concentration and activity were determined by ELISA and Zimography respectively. The data were submitted to a Mann-Whitney test and to Pearson and Spearman correlation analyses. Means and standard deviations of dental caries for the caries group were of 3.162 ± 1.385. The biofilm pH was significantly higher in the caries-free group. The CA VI activity was significantly higher in biofilm of children with caries. The CA VI concentration was significantly higher in biofilm of caries-free children. In caries-free children, there was a moderate negative correlation between CA VI activity and concentration in dental biofilm as well as between pH and CA VI activity. A negative correlation between biofilm pH and CA VI concentration was found in the caries group. In conclusion, CA VI was shown to be more active in the biofilm of school children with caries in order to contribute to neutralization of biofilm acid.

Children with a Higher Activity of Carbonic Anhydrase VI in Saliva Are More Likely to Develop Dental Caries

2017 ◽  
Vol 51 (4) ◽  
pp. 394-401 ◽  
Author(s):  
Daniele de Cassia Rodrigues Picco ◽  
Lenita Marangoni Lopes ◽  
Marcelo Rocha Marques ◽  
Sergio Roberto Peres Line ◽  
Thaís Manzano Parisotto ◽  
...  
Keyword(s):  
Dental Caries ◽  
Carbonic Anhydrase ◽  
Free Group ◽  
Flow Rate ◽  
Negative Correlation ◽  
School Children ◽  
Salivary Flow ◽  
Buffering Capacity ◽  
Salivary Flow Rate ◽  
Salivary Ph

Objective: This study aimed to analyze the concentration and activity of carbonic anhydrase (CA) VI in the saliva of school children. We investigated the relationship among caries, CA VI concentration/activity, flow rate, pH, and buffering capacity. Materials and Methods: Seventy-four school children were divided into a caries-free group and a caries group. Clinical examinations were conducted by one examiner according to World Health Organization criteria + early caries lesions. Salivary flow rate, pH, and buffering capacity were analyzed. Salivary CA VI concentration and activity were evaluated by ELISA and zymography, respectively. The data were analyzed using Student's t test and the Mann-Whitney test, and Pearson and Spearman correlation analyses were also done. In multivariate modeling, associations between variables were expressed as odds ratios. Results: The results showed that salivary flow rate, salivary pH, and BC were significantly higher in the saliva of caries-free children. Also, the salivary CA VI concentration was significantly higher in the saliva of caries-free children. The salivary CA VI activity was higher in children with caries. We found a negative correlation between BC and dental caries. Also, in the caries group we found a positive correlation between the concentration and the activity of CA VI and a negative correlation between BC and CA VI activity. A negative correlation between salivary pH and CA VI concentration was observed in the caries-free group. A high activity of CA and a low salivary flow rate were associated with dental caries. Conclusion: These results support the conclusion that dental caries is highly affected by the activity of CA VI in saliva as well as by the salivary flow rate.

scholarly journals Dental caries experience among school children of Eastern Nepal - significant caries index

2015 ◽  
Vol 12 (2) ◽  
pp. 74-77 ◽  
Author(s):  
T K Bhagat ◽  
A Shrestha
Keyword(s):  
Public Health ◽  
Dental Caries ◽  
School Children ◽  
Health Problem ◽  
Public Health Problem ◽  
Caries Experience ◽  
Who Criteria ◽  
Dental Hard Tissues ◽  
Hard Tissues ◽  
The Mean

Background: Dental caries, an infectious microbiologic disease of dental hard tissues, is a common public health problem worldwide. The distribution of dental caries studied in any population, shows that a few in the population experience a lot of decayed teeth and most do not experience any at all or experience very little.Objective: To assess the extent and severity of dental caries among 5-12 years old children of eastern Nepal using DMFT(Decayed, Missing and Filled teeth) and SiC (Significant Caries) index.Methods: Six hundred and sixteen 5-12 years old school children were examined for decayed, missing and filled teeth using WHO criteria. DMFT, dft (decayed filled teeth) and SiC Indices were calculated. Results: The mean dft and SiC* were 1.84 and 4.60 respectively, whereas mean DMFT and SiC were 0.33 and 0.92 respectively.Conclusion: SiC gives a better picture of the at risk population, hence it should be widely used along with dft/DMFT.Health Renaissance 2014;12(2): pp 74-77

scholarly journals Genetic polymorphisms in the carbonic anhydrase VI gene and dental caries susceptibility

2015 ◽  
Vol 14 (2) ◽  
pp. 5986-5993 ◽  
Author(s):  
Z.-Q. Li ◽  
X.-P. Hu ◽  
J.-Y. Zhou ◽  
X.-D. Xie ◽  
J.-M. Zhang
Keyword(s):  
Dental Caries ◽  
Carbonic Anhydrase ◽  
Genetic Polymorphisms ◽  
Dental Caries Susceptibility ◽  
Carbonic Anhydrase Vi

Fluoride Addition to Milk and its Effect on Dental Caries in School Children

1963 ◽  
Vol 17 (2) ◽  
pp. 50-58
Author(s):  
L. L. Rusoff ◽  
B. S. Konikoff ◽  
J. B. Frye ◽  
J. E. Johnston ◽  
W. W. Frye
Keyword(s):  
Dental Caries ◽  
School Children ◽  
Fluoride Addition

scholarly journals CHOP-Dependent Stress-Inducible Expression of a Novel Form of Carbonic Anhydrase VI

1999 ◽  
Vol 19 (1) ◽  
pp. 495-504 ◽  
Author(s):  
John Sok ◽  
Xiao-Zhong Wang ◽  
Nikoleta Batchvarova ◽  
Masahiko Kuroda ◽  
Heather Harding ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Target Gene ◽  
Target Genes ◽  
Direct Evidence ◽  
Nuclear Protein ◽  
Intracellular Form ◽  
Carbonic Anhydrase Vi ◽  
Responsive Element

ABSTRACT CHOP (also called GADD153) is a stress-inducible nuclear protein that dimerizes with members of the C/EBP family of transcription factors and was initially identified as an inhibitor of C/EBP binding to classic C/EBP target genes. Subsequent experiments suggested a role for CHOP-C/EBP heterodimers in positively regulating gene expression; however, direct evidence that this is the case has so far not been uncovered. Here we describe the identification of a positively regulated direct CHOP-C/EBP target gene, that encoding murine carbonic anhydrase VI (CA-VI). The stress-inducible form of the gene is expressed from an internal promoter and encodes a novel intracellular form of what is normally a secreted protein. Stress-induced expression of CA-VI is both CHOP and C/EBPβ dependent in that it does not occur in cells deficient in either gene. A CHOP-responsive element was mapped to the inducibleCA-VI promoter, and in vitro footprinting revealed binding of CHOP-C/EBP heterodimers to that site. Rescue of CA-VIexpression in c/ebpβ−/− cells by exogenous C/EBPβ and a shorter, normally inhibitory isoform of the protein known as LIP suggests that the role of the C/EBP partner is limited to targeting the CHOP-containing heterodimer to the response element and points to a preeminent role for CHOP in CA-VI induction during stress.

scholarly journals Association of Dental Caries and Anthropometric Measures among Primary School Children

Children ◽  
2021 ◽  
Vol 8 (3) ◽  
pp. 223
Author(s):  
Wajiha Anzar ◽  
Ambrina Qureshi ◽  
Ashar Afaq ◽  
Hiba F. Kattan ◽  
Basil Almutairi ◽  
...  
Keyword(s):  
Dental Caries ◽  
Primary School ◽  
Clinical Data ◽  
School Children ◽  
Low Income ◽  
Primary School Children ◽  
Anthropometric Measures ◽  
Cross Sectional ◽  
Height For Age ◽  
Weight For Age

Aim: This study aimed to investigate an association between dental caries status and anthropometric measures in primary school children. Methods and Materials: An analytical cross-sectional study (n = 376) was conducted among primary school children (age range = 6–9 years) registered in private schools. Non-clinical data was gathered from parents of participating children through a self-administered structured questionnaire as well as from the children through an interviewer-administered questionnaire. Clinical data included the examination of dental caries using dmft/DMFT index and anthropometric measures including calculated z-scores of height-for-age (HAZ), weight-for-age (WAZ), BMI-for-age (BAZ), and physical examination. Inferential statistics included Kruskal Wallis and linear regression for univariate and multivariate analysis respectively. Results: The proportion of dental caries in primary and secondary dentition was 67.6% and 8.2% respectively. A significant association was observed between dental caries status and HAZ, WAZ, and BAZ (p < 0.001). An inverse relation was found between low, medium, and high dental caries categories and anthropometric measures. Conclusions: In the primary dentition, dental caries were significantly and inversely related to weight-for-age, height-for-age, and BMI-for-age. Hence, it can be concluded that among the low-income population dental caries is associated with lower anthropometric outcomes in children and therefore caries management should be considered an approach impacting overall health and quality of life.

scholarly journals Experience and Prevalence of Dental Caries in 6 to 12-Year-Old School Children in an Agricultural Community: A Cross-Sectional Study

Children ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 99
Author(s):  
Juan José Villalobos-Rodelo ◽  
Martha Mendoza-Rodríguez ◽  
Rosalina Islas-Zarazúa ◽  
Sonia Márquez-Rodríguez ◽  
Mariana Mora-Acosta ◽  
...  
Keyword(s):  
Dental Caries ◽  
School Children ◽  
Cross Sectional Study ◽  
Caries Experience ◽  
Sectional Study ◽  
Caries Prevalence ◽  
Migrant Group ◽  
Cross Sectional ◽  
Agricultural Worker ◽  
Dmft Index

Objective: To describe the experience and prevalence of dental caries in schoolchildren aged 6–12 years belonging to agricultural manual worker households. Material and Methods: A comparative cross-sectional study was conducted in two groups of schoolchildren: One considered “children of agricultural worker migrant parents” (n = 157) and the other “children of agricultural worker non-migrant parents” (n = 164). Epidemiological indices for dental caries were calculated for primary (dmft) and permanent (DMFT) dentitions, and compared in terms of age, sex, and the Simplified Oral Hygiene Index (SOHI). Two binary logistic regression models for caries prevalence in primary and permanent dentitions were generated in Stata. Results: For primary dentition, we observed the following dmft index: Non-migrants = 1.73 ± 2.18 vs. migrants = 1.68 ± 2.14. Additionally, we recorded the following caries prevalence: Non-migrants = 59.1% vs. migrants = 51.3%. For permanent dentition, we observed the following DMFT index: Non-migrants = 0.32 ± 0.81 vs. migrants = 0.29 ± 0.95. Further, we recorded the following caries prevalence: Non-migrants = 17.6% vs. migrants = 12.8%. No differences were observed for either dentition (p > 0.05) in caries indices and their components or in caries prevalence. When both caries indices (dmft and DMFT) were combined, the non-migrant group had a higher level of caries experience than the migrant group (p < 0.05). No relationship (p > 0.05) with migrant status was observed in either multivariate models of caries prevalence. However, age did exhibit an association (p < 0.05) with caries. Only the plaque component of SOHI was associated (p < 0.05) with caries in permanent dentition. Conclusions: Although over half of school children from agricultural manual worker households had caries in either or both dentitions and a considerable proportion were untreated lesions, the prevalence levels were somewhat lower than other reports from Mexico in similar age groups. No statistically significant differences were found in caries experience or prevalence in either dentition between non-migrant and migrant groups.

Correlation between craniovertebral angle in the sagittal plane and angles and indices measured in the frontal plane at the moment of inducing forward head posture

Work ◽  
2021 ◽  
pp. 1-7
Author(s):  
Samira Molaeifar ◽  
Farzaneh Yazdani ◽  
Amin Kordi Yoosefinejad ◽  
Mohammad Taghi Karimi
Keyword(s):  
Head And Neck ◽  
Negative Correlation ◽  
Sagittal Plane ◽  
Frontal Plane ◽  
Forward Head Posture ◽  
Head Posture ◽  
Digital Devices ◽  
Moderate Negative Correlation ◽  
The Moment ◽  
Craniovertebral Angle

BACKGROUND: Forward head posture (FHP) is the most common malposition in the head and neck area. With the growing use of digital devices, the prevalence of FHP may be expected to increase dramatically. Thus far, FHP has been evaluated only in the sagittal plane. OBJECTIVE: The objective of this study was to measure angles and indices from anatomical landmarks in the frontal plane and determine the possible correlations between these variables and craniovertebral angle (CVA) as an index of FHP in the sagittal plane. METHODS: Fifty eight healthy individuals (29 men, 29 women) between 18 and 40 years old participated in this cross-sectional study. Participants were evaluated with an 8-camera motion analysis system. After markers were placed on predetermined landmarks, the participants were asked to maintain their head and neck in the neutral position for 5 seconds. Then participants induced FHP by flexing and lowering their head. The correlation between CVA and a set of angles and indices was calculated at the moment of FHP induction. RESULTS: A moderate correlation was observed between 3-D CVA and the angle formed between the sternum and both tragi for the whole sample and separately in both sexes. A moderate negative correlation was observed between 3-D CVA and height, weight, and BMI in women. A moderate negative correlation was observed between 3-D CVA and height, weight, BMI, and hours on digital devices in men. CONCLUSIONS: Changes in CVA in the sagittal plane can be predicted from changes in the angle formed between the midpoint of the sternum and the left and right tragi in the frontal plane.

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