scholarly journals RAPD and ISSR markers in the evaluation of genetic divergence among accessions of elephant grass

2011 ◽  
Vol 10 (3) ◽  
pp. 1214-1223 ◽  
Author(s):  
R.S.N de Lima ◽  
R.F. Daher ◽  
L.S.A. Gonçalves ◽  
D.A. Rossi ◽  
A.T. do Amaral Júnior ◽  
...  
Keyword(s):  
Genetic Divergence ◽  
Issr Markers ◽  
Elephant Grass ◽  
Rapd And Issr

Collection and characterization of banana gene pools (Musa spp.) in Manipur (N.E. India) using PCR–RFLP and RAPD and ISSR markers

2021 ◽  
Author(s):  
Warepam Amuchou Singh ◽  
Nandeibam Samarjit Singh ◽  
Elangbam Julia Devi ◽  
Pratap Jyoti Handique ◽  
Huidrom Sunitibala Devi
Keyword(s):  
Issr Markers ◽  
Gene Pools ◽  
Musa Spp ◽  
Pcr Rflp ◽  
Rapd And Issr

Species-diagnostic and species-specific DNA sequences evenly distributed throughout pine and spruce chromosomes

Genome ◽  
2010 ◽  
Vol 53 (10) ◽  
pp. 769-777 ◽  
Author(s):  
Melanie Mehes-Smith ◽  
Paul Michael ◽  
Kabwe Nkongolo
Keyword(s):  
Dna Sequences ◽  
Random Amplified Polymorphic Dna ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Pinus Monticola ◽  
The Family ◽  
Species Specific ◽  
Rapd And Issr ◽  
Specific Sequences

Genome organization in the family Pinaceae is complex and largely unknown. The main purpose of the present study was to develop and physically map species-diagnostic and species-specific molecular markers in pine and spruce. Five RAPD (random amplified polymorphic DNA) and one ISSR (inter-simple sequence repeat) species-diagnostic or species-specific markers for Picea mariana , Picea rubens , Pinus strobus , or Pinus monticola were identified, cloned, and sequenced. In situ hybridization of these sequences to spruce and pine chromosomes showed the sequences to be present in high copy number and evenly distributed throughout the genome. The analysis of centromeric and telomeric regions revealed the absence of significant clustering of species-diagnostic and species-specific sequences in all the chromosomes of the four species studied. Both RAPD and ISSR markers showed similar patterns.

scholarly journals Molecular analysis of genetic diversity in population of Silybum marianum (L.) Gaertn in Egypt

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Marwa Hamouda
Keyword(s):  
Genetic Diversity ◽  
Electrophoretic Pattern ◽  
Issr Markers ◽  
Silybum Marianum ◽  
Inter Simple Sequence Repeats ◽  
Sds Page ◽  
Dna Pattern ◽  
Pharmaceutical Properties ◽  
High Level ◽  
Rapd And Issr

Abstract Background Silybum marianum L. Gaertn is a medicinal plant of unique pharmaceutical properties in the treatment of liver disorders and diabetic nephropathy. Biochemical (SDS-PAGE) and molecular markers such as randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) technologies were used in this work to detect genetic diversity of 14 collections of Silybum marianum population in Egypt. Results The electrophoretic pattern of seed protein gave different molecular weight bands, ranging from 24 to 111 KDa with the presence of unique bands. RAPD results revealed a high level of polymorphism (73.2%) using 12 RAPD primers, but only eight of them gave reproducible polymorphic DNA pattern. Sixteen primers were used in the ISSR method; only ten of them yielded clearly identifiable bands. The percentage of polymorphism is about 80% of the studied samples. Conclusion The obtained data confirmed that SDS-protein, RAPD, and ISSR markers are important tools for genetic analysis for Silybum marianum and recommended to give accurate results.

scholarly journals Efficiency of improved RAPD and ISSR markers in assessing genetic diversity and relationships in Angelica sinensis (Oliv.) Diels varieties of China

2015 ◽  
Vol 18 (2) ◽  
pp. 96-102 ◽  
Author(s):  
Zhiqiang Mei ◽  
Chun Zhang ◽  
Md. Asaduzzaman Khan ◽  
Ye Zhu ◽  
Mousumi Tania ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Issr Markers ◽  
Angelica Sinensis ◽  
Rapd And Issr

A Comparative Analysis of Molecular Diversity of Erect Milkvetch (Astragalus adsurgens) Germplasm from North China Using RAPD and ISSR Markers

2009 ◽  
Vol 47 (1-2) ◽  
pp. 92-99 ◽  
Author(s):  
Lin-kai Huang ◽  
Zhi-hong Chen ◽  
Xin-quan Zhang ◽  
Zhi-gang Wang ◽  
Chou-sheng Liu
Keyword(s):  
Comparative Analysis ◽  
Molecular Diversity ◽  
North China ◽  
Issr Markers ◽  
Astragalus Adsurgens ◽  
Rapd And Issr

scholarly journals Characterization of genetic structure of alfalfa (Medicago sp.) from trans-Himalaya using RAPD and ISSR markers

2011 ◽  
Vol 10 (42) ◽  
pp. 8176-8187 ◽  
Author(s):  
Raj Xavier Janifer ◽  
Kumar Jitendra ◽  
Bihari Srivastava Ravi
Keyword(s):  
Genetic Structure ◽  
Issr Markers ◽  
Rapd And Issr

scholarly journals Genetic Relationships Among Representatives of Dasypyrum, Secale and Triticum Species Revealed with RAPD and ISSR Markers

2014 ◽  
Vol 42 (2) ◽  
pp. 420-430
Author(s):  
Agnieszka GRADZIELEWSKA ◽  
Mirosław TYRKA ◽  
Justyna LESNIOWSKA-NOWAK ◽  
Justyna NAZARUK
Keyword(s):  
Gene Diversity ◽  
Genetic Relationships ◽  
Issr Markers ◽  
Genetic Distances ◽  
Fixation Index ◽  
Triticum Species ◽  
The Difference ◽  
Bridge Species ◽  
Differentiation Gene ◽  
Rapd And Issr

In this study the genetic similarity among Dasypyrum, Secale andTriticum species with RAPDs and ISSRs was analyzed. To show a level of similarity between the species, 12 populations of Dasypyrum (11 D. villosum and 1 D. breviaristatum), together with 12 accessions belonging to 3 Secale species and 12 accessions from 4 Triticum species were used. Genetic distances (GD) and bootstrap values were calculated and PCA analysis was conducted to present the relationships among the species. To estimate the genetic structure among and inside genera, as well as population differentiation, gene diversity (He), total genetic variation (Ht) and Wright’s fixation index (Fst) were computed. The highest values were found in Triticum, within which Ht was equal to 0.332±0.023 and Fst was 0.42. It confirmed that the material studied was highly differentiated. Both systems found Dasypyrum more related with Triticum, as compared to Secale. With RAPDs, genetic distance (GD) between Triticum and Dasypyrum was 0.435, respectively 0.460 for Secale vs Dasypyrum. In the case of ISSRs these values were 0.374 and 0.407, respectively. Despite the fact that the difference between the two GD indices was insignificant, one should not exclude the possibility of successful hybridization of Dasypyrum and Secale, especially when using bridge species.

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