Factors influencing large-scale micropropagation of Sphagneticola calendulacea (L.) Pruski and clonality assessment using RAPD and ISSR markers

Genome organization in the family Pinaceae is complex and largely unknown. The main purpose of the present study was to develop and physically map species-diagnostic and species-specific molecular markers in pine and spruce. Five RAPD (random amplified polymorphic DNA) and one ISSR (inter-simple sequence repeat) species-diagnostic or species-specific markers for Picea mariana , Picea rubens , Pinus strobus , or Pinus monticola were identified, cloned, and sequenced. In situ hybridization of these sequences to spruce and pine chromosomes showed the sequences to be present in high copy number and evenly distributed throughout the genome. The analysis of centromeric and telomeric regions revealed the absence of significant clustering of species-diagnostic and species-specific sequences in all the chromosomes of the four species studied. Both RAPD and ISSR markers showed similar patterns.

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Molecular analysis of genetic diversity in population of Silybum marianum (L.) Gaertn in Egypt

Journal of Genetic Engineering and Biotechnology ◽

10.1186/s43141-019-0011-6 ◽

2019 ◽

Vol 17 (1) ◽

Cited By ~ 1

Author(s):

Marwa Hamouda

Keyword(s):

Genetic Diversity ◽

Electrophoretic Pattern ◽

Issr Markers ◽

Silybum Marianum ◽

Inter Simple Sequence Repeats ◽

Sds Page ◽

Dna Pattern ◽

Pharmaceutical Properties ◽

High Level ◽

Rapd And Issr

Abstract Background Silybum marianum L. Gaertn is a medicinal plant of unique pharmaceutical properties in the treatment of liver disorders and diabetic nephropathy. Biochemical (SDS-PAGE) and molecular markers such as randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) technologies were used in this work to detect genetic diversity of 14 collections of Silybum marianum population in Egypt. Results The electrophoretic pattern of seed protein gave different molecular weight bands, ranging from 24 to 111 KDa with the presence of unique bands. RAPD results revealed a high level of polymorphism (73.2%) using 12 RAPD primers, but only eight of them gave reproducible polymorphic DNA pattern. Sixteen primers were used in the ISSR method; only ten of them yielded clearly identifiable bands. The percentage of polymorphism is about 80% of the studied samples. Conclusion The obtained data confirmed that SDS-protein, RAPD, and ISSR markers are important tools for genetic analysis for Silybum marianum and recommended to give accurate results.

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Efficiency of improved RAPD and ISSR markers in assessing genetic diversity and relationships in Angelica sinensis (Oliv.) Diels varieties of China

Electronic Journal of Biotechnology ◽

10.1016/j.ejbt.2014.12.006 ◽

2015 ◽

Vol 18 (2) ◽

pp. 96-102 ◽

Cited By ~ 11

Author(s):

Zhiqiang Mei ◽

Chun Zhang ◽

Md. Asaduzzaman Khan ◽

Ye Zhu ◽

Mousumi Tania ◽

...

Keyword(s):

Genetic Diversity ◽

Issr Markers ◽

Angelica Sinensis ◽

Rapd And Issr

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A Comparative Analysis of Molecular Diversity of Erect Milkvetch (Astragalus adsurgens) Germplasm from North China Using RAPD and ISSR Markers

Biochemical Genetics ◽

10.1007/s10528-008-9209-z ◽

2009 ◽

Vol 47 (1-2) ◽

pp. 92-99 ◽

Cited By ~ 2

Author(s):

Lin-kai Huang ◽

Zhi-hong Chen ◽

Xin-quan Zhang ◽

Zhi-gang Wang ◽

Chou-sheng Liu

Keyword(s):

Comparative Analysis ◽

Molecular Diversity ◽

North China ◽

Issr Markers ◽

Astragalus Adsurgens ◽

Rapd And Issr

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Genetic diversity analysis using RAPD and ISSR markers revealed discrete genetic makeup in relation to fibre and oil content in Linum usitatissimum L. genotypes

The Nucleus ◽

10.1007/s13237-017-0206-7 ◽

2017 ◽

Vol 61 (1) ◽

pp. 45-53 ◽

Cited By ~ 2

Author(s):

Arpna Kumari ◽

Satish Paul ◽

Vikas Sharma

Keyword(s):

Genetic Diversity ◽

Linum Usitatissimum ◽

Oil Content ◽

Issr Markers ◽

Diversity Analysis ◽

Genetic Diversity Analysis ◽

Linum Usitatissimum L ◽

Genetic Makeup ◽

Rapd And Issr

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Analysis of Factors Influencing Effective Utilization Coefficient of Irrigation Water in the Manas River Basin

Water ◽

10.3390/w13020189 ◽

2021 ◽

Vol 13 (2) ◽

pp. 189

Author(s):

Lili Yang ◽

Tong Heng ◽

Guang Yang ◽

Xinchen Gu ◽

Jiaxin Wang ◽

...

Keyword(s):

Irrigation Water ◽

Large Scale ◽

Grey Model ◽

Analytic Hierarchy ◽

Effective Utilization ◽

Factors Affecting ◽

Factors Influencing ◽

Utilization Coefficient ◽

Using Data ◽

Effective Use

The factors influencing the effective utilization coefficient of irrigation water are not understood well. It is usually considered that this coefficient is lower in areas with large-scale irrigation. With this background, we analyzed the effective utilization coefficient of irrigation water using the analytic hierarchy process using data from 2014 to 2019 in Shihezi City, Xinjiang. The weights of the influencing factors on the effective utilization coefficient of irrigation water in different irrigation areas were analyzed. Predictions of the coefficient’s values for different years were made by understanding the trends based on the grey model. The results show that the scale of the irrigation area is not the only factor determining the effective utilization coefficient of irrigation water. Irrigation technology, organizational integrity, crop types, water price management, local economic level, and channel seepage prevention are the most critical factors affecting the effective use of irrigation water. The grey model prediction results show that the effective utilization coefficient of farmland irrigation water will continuously increase and reach 0.7204 in 2029. This research can serve as a reference for government authorities to make scientific decisions on water-saving projects in irrigation districts in terms of management, operation, and investment.

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