scholarly journals Investigation of Dientamoeba fragilis Frequency in Faecal Samples of Patients with Enterobius vermicularis Infection by Polymerase Chain Reaction

2021 ◽  
Vol 45 (3) ◽  
pp. 195-200
Author(s):  
İbrahim Yıldız ◽  
Sema Ertuğ ◽  
Evren Tileklioğlu ◽  
Erdoğan Malatyalı ◽  
Özgür Güçlü ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Enterobius Vermicularis ◽  
Chain Reaction ◽  
Dientamoeba Fragilis ◽  
Faecal Samples ◽  
Polymerase Chain

Prevalence of Tritrichomonas foetus infection in cats with diarrhoea in the UK

2007 ◽  
Vol 9 (3) ◽  
pp. 214-218 ◽  
Author(s):  
Danièlle A. Gunn-Moore ◽  
Theresa M. McCann ◽  
Nicki Reed ◽  
Kerry E. Simpson ◽  
Bryn Tennant
Keyword(s):  
Polymerase Chain Reaction ◽  
Tritrichomonas Foetus ◽  
Chain Reaction ◽  
Faecal Samples ◽  
The Usa ◽  
Polymerase Chain ◽  
The Uk

Faecal samples from 111 cats with diarrhoea that were living in the UK were submitted for the assessment of Tritrichomonas foetus infection by polymerase chain reaction (PCR). Sixteen (14.4%) samples were found to be positive. In agreement with studies from the USA, infected cats were predominantly of a year of age or less and of a pedigree breed, with Siamese and Bengal cats specifically over-represented in this population.

Comparison of different diagnostic test to detect feline panleukopenia virus among cats in Kerala, India

2016 ◽  
Author(s):  
Koulath P. Raheena ◽  
P. M. Priya ◽  
Binu K Mani ◽  
M. Mini ◽  
Usha Narayana Pillai
Keyword(s):  
Polymerase Chain Reaction ◽  
Statistical Analysis ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Feline Panleukopenia Virus ◽  
Acute Viral Infection ◽  
Faecal Samples ◽  
Wild Felids ◽  
Polymerase Chain ◽  
Strip Test

Feline panleukopenia (FPL) is an acute viral infection of domestic and wild felids causing high mortality among non immune kittens. Commercially available immunochromatographic (IC) strips, haemagglutination (HA) test and polymerase chain reaction (PCR) were employed to detect FPLV. In the current study IC strip test and HA test were compared with PCR. A total of 27 faecal samples from cats clinically suspected for FPL infection were collected from five districts of Kerala, India. Out of 27 samples tested, 10 were positive by IC strips, while 8 and 21 samples were found positive by HA test and PCR, respectively. On statistical analysis, specificity of IC strip and HA test versus PCR was excellent (100 per cent), whereas sensitivity was poor. In comparison with PCR, sensitivity for IC strip test and HA test was 47.6 per cent and 38.1 per cent, respectively. The study showed PCR assay as a sensitive, specific and rapid technique for FPLV detection in cats using faecal specimens.

Detection of Helicobacter pylori cagA gene by polymerase chain reaction in faecal samples

1999 ◽  
Vol 11 (3) ◽  
pp. 251-256 ◽  
Author(s):  
Francesco Russo ◽  
Maria Notarnicola ◽  
Giovanni Di Matteo ◽  
Claudio Leoci ◽  
Maria Lucia Caruso ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Helicobacter Pylori ◽  
Caga Gene ◽  
Chain Reaction ◽  
Faecal Samples ◽  
Polymerase Chain

scholarly journals Prevalence and characteristics ofEscherichia coilserotype O157:H7 and other verotoxin-producingE. coliin healthy cattle

1996 ◽  
Vol 117 (2) ◽  
pp. 251-257 ◽  
Author(s):  
M. Blanco ◽  
J. E. Blanco ◽  
J. Blanco ◽  
E. A. Gonzalez ◽  
A. Mora ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Gene Sequence ◽  
Heterogeneous Population ◽  
Chain Reaction ◽  
E Coli ◽  
Healthy Cattle ◽  
Faecal Samples ◽  
Polymerase Chain

SummaryFrom February to July of 1994, 328 faecal samples from 32 herds were collected and verotoxin-producingEscherichia coli(VTEC) found on 84% of the farms. The proportion of animals infected varied from 0–63%. VTEC were recovered from 52 (20%) of 257 cows and from 16 (23%) of 71 calves. Although the VTEC belonged to 25 different serogroups, 7 (O8. O20, O22, O77, O113, O126 and O162) accounted for 46% of strains. Nearly 45% of the 83 bovine VTEC strains belonged to serogroups associated with haemorrhagic colitis and haemolytic uraernic syndrome in humans. However, only 2 (2%) of 83 VTEC strains isolated from cattle belonged to enterohaemorrhagicE. coli(EHEC) serotypes (O26:H11 and O157:H7), and only 8 (10%) were positive for the attaching and effacingE. coli (eae)gene sequence. Polymerase chain reaction (PCR) showed that 17 (20%) of VTEC strains carried VT1 genes. 43 (52%) possessed VT2 genes, and 23 (28%) carried both VT1 and VT2 genes. Characterization of VTEC isolates revelated a heterogeneous population in terms of serogroup and toxin type in the positive herds. This study confirms that healthy cattle are a reservoir of VTEC, but, the absence ofeaegenes in most bovine VTEC strains suggests that they may be less virulent for humans thaneae-positive EHEC.

A survey ofCampylobacterspecies shed in faeces of beef cattle using polymerase chain reaction

2003 ◽  
Vol 49 (11) ◽  
pp. 655-661 ◽  
Author(s):  
G D Inglis ◽  
L D Kalischuk ◽  
H W Busz
Keyword(s):  
Polymerase Chain Reaction ◽  
Beef Cattle ◽  
Internal Control ◽  
Extraction Procedure ◽  
Individual Species ◽  
Chain Reaction ◽  
Faecal Samples ◽  
Two Samples ◽  
Polymerase Chain ◽  
Campylobacter Species

A polymerase chain reaction (PCR)-based survey of campylobacters associated with faeces collected from 382 beef cattle was undertaken. To ensure the removal of PCR inhibitors present in faeces and determine if adequate extraction was achieved, faeces were seeded with internal control DNA (i.e., DNA designed to amplify with the Campylobacter genus primer set, but provide a smaller amplicon) before the extraction procedure. In only two samples (0.5%) were the internal control or Campylobacter genus amplicons not detected. In the remaining 380 faecal samples, Campylobacter DNA was detected in 83% of the faecal samples (80% of the faecal samples were positive for Campylobacter genus DNA, and 3% of the samples were negative for Campylobacter genus DNA but positive for DNA of individual species). The most frequently detected species was Campylobacter lanienae (49%), a species only recently connected to livestock hosts. Campylobacter jejuni DNA was detected in 38% of the faecal samples, and Campylobacter hyointestinalis and Campylobacter coli DNA were detected in 8% and 0.5% of the samples, respectively. Campylobacter fetus DNA was not detected. Twenty-four percent of the faecal samples contained DNA of at least two species of Campylobacter. Of these samples, the majority (81%) contained DNA of C. jejuni and C. lanienae. The results of this study indicate that beef cattle commonly release a variety of Campylobacter species into the environment and may contribute to the high prevalence of campylobacteriosis in humans inhabiting areas of intensive cattle production, such as southern Alberta. Furthermore, this study demonstrates the utility of using PCR as a rapid and accurate method for simultaneously detecting the DNA of a diverse number of Campylobacter species associated with bovine faeces.Key words: campylobacters, detection, technique, Bos taurus.

Sorbitol non-fermenting shiga toxin-producingEscherichia coliin cattle on smallholdings

2014 ◽  
Vol 143 (1) ◽  
pp. 94-103 ◽  
Author(s):  
M. Z. ISLAM ◽  
J. P. CHRISTENSEN ◽  
P. K. BISWAS
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Shiga Toxin ◽  
Gel Electrophoresis ◽  
Virulence Genes ◽  
Pulsed Field Gel Electrophoresis ◽  
Chain Reaction ◽  
E Coli ◽  
Faecal Samples ◽  
Polymerase Chain

SUMMARYWe investigated faecal samples collected from the rectum of 518 cattle on 371 randomly selected smallholdings in Bangladesh for the presence of sorbitol non-fermenting (SN-F) shiga toxin-producingEscherichia coli(STEC). The SN-F isolates were tested for the presence ofrfbO157,stx1, stx2, eaeandhlyAgenes by polymerase chain reaction (PCR). Seven SN-F isolates lacking these genes were profiled by pulsed-field gel electrophoresis (PFGE) to verify their clonality. SN-FE. coliwas identified in 44 [8·5%, 95% confidence interval (CI) 6·4–11·2] samples; of these, 28 (5·4%, 95% CI 3·8–7·7) had shiga toxin-producing strains, although only two carried therfbO157 gene. Thirteen isolates carried thehlyAgene while 18 harboured theeaegene. Based on PFGE, six pulsotypes were observed among the seven isolates that had no virulence genes. To the best of our knowledge this is the first report on shiga toxin-producingE. colifrom direct rectal faecal samples of cattle on smallholdings.

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