scholarly journals Distribution of genes encoding adhesins and biofilm formation capacity among Uropathogenic Escherichia coli isolates in relation to the antimicrobial resistance

2020 ◽  
Vol 20 (1) ◽  
pp. 238-247
Author(s):  
Ashraf A Kadry ◽  
Nour M Al-Kashef ◽  
Amira M El-Ganiny
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Antimicrobial Resistance ◽  
Biofilm Formation ◽  
Critical Role ◽  
Uropathogenic Escherichia Coli ◽  
Diffusion Method ◽  
Esbl Production ◽  
Microtitre Plate Assay ◽  
Genes Encoding

Background: Escherichia coli is the most predominant pathogen involved in UTIs. Mainly, fimbrial surface appendages are impli- cated in adherence to urothelium besides non-fimbrial proteins. Objectives: To determine prevalence of genes encoding fimbrial and non-fimbrial proteins among Uropathogenic Escherichia coli (UPEC). Furthermore, distribution of these genes and biofilm formation capacity were investigated in relation to antimicrobial resistance. Methods: Antimicrobial susceptibility of 112 UPEC isolates was performed using disc diffusion method. ESBL production was confirmed by double disc synergy test. Genes encoding fimbrial and non-fimbrial proteins were detected using PCR and biofilm formation was investigated using microtitre plate assay. Results: UPEC isolates exhibited high resistance against doxycyclines (88.39 %), β-lactams (7.14-86.6%), sulphamethoxaz- ole–trimethoprim (53.75%) and fluoro-quinolones (50%). Fifty percent of tested isolates were ESBL producers. PapGII gene was statistically more prevalent among pyelonephritis isolates. SfaS, focG and picU genes were statistically associated with flu- oro-quinolone (FQs) sensitive isolates and Dr/afaBC gene was statistically associated with ESBL production. Moreover, non- MDR isolates produced sturdier biofilm. Conclusion: PapGII adhesin variant seems to have a critical role in colonization of upper urinary tract. There is a possible link between antimicrobial resistance and virulence being capable of affecting the distribution of some genes besides its negative impact on biofilm formation. Keywords: Urinary tract infection; Escherichia coli; UPEC; adhesin genes; ESBL; biofilm.  

scholarly journals Implication of biofilm formation in the persistence of urinary tract infection caused by uropathogenic Escherichia coli

2006 ◽  
Vol 12 (10) ◽  
pp. 1034-1036 ◽  
Author(s):  
S.M. Soto ◽  
A. Smithson ◽  
J.P. Horcajada ◽  
J.A. Martinez ◽  
J.P. Mensa ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Biofilm Formation ◽  
Uropathogenic Escherichia Coli

Urinary tract infection in Uruguayan children: Aetiology, antimicrobial resistance and uropathogenic Escherichia coli virulotyping

2014 ◽  
Vol 2 (4) ◽  
pp. 293-298 ◽  
Author(s):  
Luciana Robino ◽  
Virginia García-Fulgueiras ◽  
Lucía Araujo ◽  
Gabriela Algorta ◽  
Maria Catalina Pírez ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Antimicrobial Resistance ◽  
Tract Infection ◽  
Uropathogenic Escherichia Coli

scholarly journals Antibiotic susceptibility profile and extended spectrum β-lactamases production by uropathogenic Escherichia coli from tertiary care hospital of rural settings

2018 ◽  
Vol 6 (12) ◽  
pp. 4022
Author(s):  
Mustafa Sofiur Rahman ◽  
Ritu Garg ◽  
Varsha A. Singh ◽  
Dipankar Biswas
Keyword(s):  
Escherichia Coli ◽  
Drug Resistance ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Antibiotic Susceptibility ◽  
Uropathogenic Escherichia Coli ◽  
Diffusion Method ◽  
Care Hospital ◽  
Antibiotic Susceptibility Test ◽  
Tract Infections

Background: Escherichia coli are the most common cause of urinary tract infections in community as well as hospital settings. Emergence of drug resistance in Escherichia coli due to various mechanisms makes the treatment options very limited. This study was undertaken to detect ESBLs in uropathogenic Escherichia coli isolates and to determine their antimicrobial susceptibility pattern in rural setting.Methods: A prospective study was done on 502 E. coli isolates from clinically suspected cases of urinary tract infections (UTI) patients of all age groups. All samples were inoculated on Cysteine Lactose Electrolyte Deficient Agar (CLED). Organisms grown in pure culture were identified by standard biochemical tests. Antibiotic susceptibility test was done by the Kirby Bauer Disc diffusion method on Muller Hinton agar. ESBL detection was done as per CLSI guidelines.Results: Of the 502 isolates of Escherichia coli, nitrofurantoin (82%) was found be most sensitive antimicrobial followed by amikacin (73%), gentamycin (71%) and imipenem (64%). Common empirically used antibiotics like fluroquinolones and Cotrimoxazole drugs showed alarming rate of resistance. 60% isolates were found to be multidrug resistant. ESBL production was detected in 31% isolates. ESBL producing strains were found to be more drug resistant than non ESBL producing strains.Conclusions: So, drug resistance due to production of ESBLs in Escherichia coli is a serious threat for clinicians. Strict infection control measures and early detection of beta lactamase producing isolates are the need of the hour to contain the emergence of this type of resistance.

scholarly journals Antibiotic resistance pattern of uropathogenic Escherichia coli isolated from children with symptomatic urinary tract infection in Moscow, Russia

2021 ◽  
pp. 212-219
Author(s):  
Souadkia Sarra ◽  
Mbarga Manga Joseph Arsene ◽  
Volina Elena Grigorievna ◽  
Podoprigora Irina Victorovna ◽  
Yashina Natalia Vyacheslavovna ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Statistical Significance ◽  
Rank Correlation ◽  
Uropathogenic Escherichia Coli ◽  
Diffusion Method ◽  
Correlation Test ◽  
Spearman’S Rank Correlation ◽  
Tract Infections

Background and Aim: Uropathogenic Escherichia coli (UPEC) is commonly involved in urinary tract infections (UTIs), which are generally treated with antibiotics. However, the emergence of multidrug-resistant (MDR) strains of UPEC has made the treatment difficult. There is thus a need to continuously assess their sensitivity to antibiotics. This study aimed to determine the antibiotic resistance patterns and MDR phenotypes of UPEC strains isolated from children diagnosed with UTIs at the Russian Children's Clinical Hospital in Moscow, Russia. Materials and Methods: Kirby–Bauer's disc diffusion method was used to study the sensitivity to antibiotics of 106 UPEC isolates from urine specimens from children (aged from 9 months to 18 years old) diagnosed with UTIs. The results were interpreted in accordance with the Clinical and Laboratory Standards Institute guidelines and the correlations of variables with the degree to which each antibiotic inhibited the UPEC strains in terms of diameter on the disc were determined using Spearman's rank correlation test. A t-test and principal component analysis were performed to visualize the correlations of the susceptibility of UPEC to antibiotics with the age and sex of the patients. Statistical significance was set at p≤0.05. Results: Among the 106 UPEC strains tested, none (0%) showed resistance to fosfomycin (FO), while 84 (79.2%) were resistant (R) to at least one antibiotic. The highest rates of resistance were observed to amoxicillin (69.8%), ampicillin (62.3%), cefazolin (39.6%), trimethoprim (TR) (37.7%), ceftriaxone (34.9%), and tetracycline (33.0%). Interestingly, 22 (20.8%) strains were R to imipenem. UPEC isolates from males aged 1-6 years were more R to antibiotics than those from the other groups, with the exception of TR, to which UPEC isolates from females aged 13-18 years old were less sensitive (S). The multidrug-resistance (MDR) index ranged between 0.00 and 0.75 and we found that more than a quarter of UPEC (31/106) had an MDR index ≥0.5 and only 22 (20.7%) strains were S to all antibiotics tested (MDR index=0). Finally, Spearman's rank correlation test showed that, with the exception of FO, there were correlations between the inhibition diameters of all other antibiotics. Conclusion: FO is the only antibiotic to which all UPECs were S and may be suggested as the first line of treatment for UPEC. Further research is needed to continue monitoring antibiotic resistance and to investigate the genetic features associated with such resistance observed in this study.

scholarly journals A newly identified group of P-like (PL) fimbriae from extra-intestinal pathogenic Escherichia coli (ExPEC) encode distinct adhesin subunits and mediate adherence to host cells

2021 ◽  
Author(s):  
Charles M. Dozois ◽  
Hajer Habouria ◽  
Hicham Bessaiah ◽  
Julie Buron ◽  
Sébastien Houle
Keyword(s):  
Escherichia Coli ◽  
Biofilm Formation ◽  
Critical Role ◽  
Sequence Divergence ◽  
Host Cells ◽  
Genomic Databases ◽  
Genes Encoding ◽  
Adhesin Protein ◽  
Fimbrial Adhesins

Fimbrial adhesins play a critical role for bacterial adherence and biofilm formation. Sequencing of avian pathogenic Escherichia coli (APEC) strain QT598 identified a fimbrial gene cluster belonging to the π group that wenamed PL (P-like) fimbriae, since genetic organization and sequence are similar to Pap and related fimbriae. Screening of genomic databases indicated that genes encoding PL fimbriae located on IncF plasmids are present in a diversity of E. coli isolates from poultry, human systemic infections, and other sources. As with P fimbriae, PL fimbriae exhibit sequence divergence in adhesin encoding genes, and strains could be divided into 5 classes based on differences in sequences of the PlfG adhesin protein. The plf genes from two predominant PlfG adhesin classes, PlfG-I and PlfG-II were cloned. PL fimbriae were visualized by electron microscopy, promoted biofilm formation, demonstrated distinct hemagglutination profiles and promoted adherence to human bladder and kidney epithelial cell lines. Hybrid fimbriae comprised of genes from plfQT598 wherein plfG was replaced by papG encoding adhesin genes were also shown to be functional and mediate adherence to epithelial cells, further indicating similarity and functional compatibility between these two types of fimbriae. Although deletion of plf genes did not significantly reduce colonization of the mouse urinary tract, plf gene expression was increased over 40-fold in the bladder compared to during in vitro culture. Overall, PL fimbriae represent a new group of fimbriae demonstrating both functional differences and similarities to P fimbriae and may contribute to adherence to cells and colonization of host tissues.

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