scholarly journals Phytochemical screening and antibacterial activity of Garcinia kola seed extracts against pathogenic methicillin resistant Staphylococcus aureus

2018 ◽  
Vol 10 (2) ◽  
pp. 198
Author(s):  
F.S. Nas ◽  
A.U. Zage ◽  
M Ali
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Phytochemical Screening ◽  
Methicillin Resistant ◽  
Garcinia Kola ◽  
Seed Extracts

scholarly journals In Vitro Antibacterial Activity of Crude Extracts of Artocarpus heterophyllus Seeds against Selected Diarrhoea-Causing Superbug Bacteria

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Asio Eve ◽  
Adamu Almustapha Aliero ◽  
Doreen Nalubiri ◽  
Rasheed Omotayo Adeyemo ◽  
Saheed Adekunle Akinola ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Salmonella Typhimurium ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Artocarpus Heterophyllus ◽  
Methicillin Resistant ◽  
Crude Extracts

The current upsurge in resistance to conventional antibiotics, as well as high cost of orthodox medical treatment, called for the use of medicinal plants as an alternative therapy. This research was aimed at determining the antibacterial activity of Artocarpus heterophyllus seed extracts (Jackfruit as it is locally called) in the treatment of diarrhoea. Ethanolic and hexanolic seed crude extracts of the plant were screened for antidiarrhoeal activity against bacteria isolated from clinical samples (methicillin-resistant and susceptible Staphylococcus aureus, multidrug-resistant Pseudomonas aeruginosa, ciprofloxacin-resistant Salmonella typhimurium, and third-generation cephalosporin-resistant Escherichia coli). Plant phytochemical screening was conducted using standard methods. The antibacterial activity was carried out using the agar well diffusion method and compared to the standard antibiotics ceftriaxone and vancomycin. The minimum inhibitory concentration was determined by the microbroth dilution method, whereas the minimum bactericidal concentration was determined by plating out from microtitre plates with no visible growth. The results of phytochemical screening revealed the presence of tannins, flavonoids, reducing sugars, cardiac glycosides, saponins, and steroids from the prepared crude extracts. The ethanolic and hexanolic extracts had activity on multidrug-resistant Pseudomonas aeruginosa, methicillin-resistant Staphylococcus aureus, and methicillin-susceptible Staphylococcus aureus with the mean and standard error zone of inhibition that ranged from 8.5 ± 0.5 to 16.5 ± 0.25 mm; however, the extracts were found not to have activity on resistant E. coli and Salmonella typhimurium. The ethanolic crude extract had the lowest MIC and MBC values of 31.25 and 125 mg/ml, respectively, compared to the hexane extract which had the MIC and MBC values of 62.50 and 250 mg/ml, respectively. This provides the evidence for its usage as an alternative herbal remedy for the treatment of diarrhoea caused by susceptible and methicillin-resistant Staphylococcus aureus and multidrug resistant Pseudomonas aeruginosa.

scholarly journals Phytochemical screening and antibacterial activity of Garcinia kola (hackel) and Cola nitida (vent) extracts

2020 ◽  
Vol 39 (2) ◽  
pp. 379-385
Author(s):  
B.T. Afolabi ◽  
G.C. Agu ◽  
I.B. Onajobi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Aqueous Extracts ◽  
Phytochemical Screening ◽  
Garcinia Kola ◽  
Test Plants ◽  
Cola Nitida

This study was designed to evaluate the phytochemical and antibacterial properties of Garcina kola (Orogbo) and Cola nitida (Obi) extracts. Fresh seeds of Cola nitida and Garcinia kola were collected from Ago-Iwoye market, Ogun State. The ethanolic and aqueous extracts of the test plants were used against selected test organisms, Streptococcus pneumonia, Staphylococcus aureus, Salmonella typhi, Pseudomonas euriginosa and Escherichia coli. Disc diffusion method was adopted to test for susceptibility of the selected test bacteria to the extracts. Minimum inhibition concentration (MIC) and Minimum bactericidal concentration (MBC) were determined. Bioassay data were statistically analyzed using two-way ANOVA. The result of the phytochemical screening confirmed the presence of flavonoid, phenol, alkaloid, saponins, tannins and phlobatanins. The result of the antibacterial activity showed that the ethanolic extract of the test plants recorded highest antimicrobial activity against test isolates compared to aqueous extracts. The tested plant seeds of both plants posses reasonable antibacterial activity but to varied zones of inhibition, with Staphylococcus aureus isolate having the highest inhibitory zones (21.33) mm while Pseudomonas aeuruginosa had the least inhibitory zone (6.00) mm. The antibacterial activity were however found to be concentration dependent (Fvalue= 3.996, Pvalue= < 0.05). All tested organisms were found to have definite MIC and MBC activities which ranges between 125 and 1000μg/ml for MIC except for Kola nitida that has no definite MBC below 1000μg/ml. The result confirms the potential of antibacterial activity of Garcinia kola and Cola nitida extracts. Keywords: Antimicrobial, Cola nitida, Garcinia kola, Phytochemical, Plant extracts

scholarly journals In-vitro antibacterial activity of the seed extract of three-member Artocarpus towards Methicillin-Resistant Staphylococcus aureus (MRSA)

2020 ◽  
Vol 9 (2) ◽  
pp. 128-135
Author(s):  
Muhammad Evy Prastiyanto ◽  
Inas Hasna Azizah ◽  
Hafizha Dara Haqi ◽  
Bagus Dwi Yulianto ◽  
Aulia Bella Agmala ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Seed Extract ◽  
Methicillin Resistant ◽  
Biological Source ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay ◽  
Seed Extracts

Methicillin-Resistant Staphylococcus aureus (MRSA) infections have created a critical need for the development of natural antibacterials from a biological source. This research aimed to investigate the antibacterial activity of the seed extract of three-member Artocarpus (Artocarpus heterophyllus, A. champeden, and A. camansi) against MRSA which are the most prevalent causes of infections in patients. Crude seed extracts of three-member Artocarpus were evaluated for their antibacterial activity against MRSA. The antibacterial activity against MRSA of the three extracts was assayed in vitro by the agar well diffusion assay and agar microdilution method and minimum bactericidal concentration. The antibacterial activity, calculated as a zone of inhibition and MIC, MBC values. The Crude seed extracts of three-member Artocarpus showed antibacterial activity against the MRSA in the agar well diffusion assay (1.5-9 mm inhibition diameter). The MIC value of extract showed at 15.62 mg/mL and the MBC value of seed extract of A. heterophyllus at 62.5 mg/mL, A. champeden at 31.25 mg/mL, A. camansi at 250 mg/mL. All seed extracts have the potential to be developed as antibacterial agents, particularly against MRSA strain. Studies on the antibacterial activity against MRSA can provide new information about the benefits seed of members of Artocarpus as a source of natural antibacterial.

Antibacterial Activity of Ecklonia cava Against Methicillin-Resistant Staphylococcus aureus and Salmonella spp.

2010 ◽  
Vol 7 (4) ◽  
pp. 435-441 ◽  
Author(s):  
Jang-Gi Choi ◽  
Ok-Hwa Kang ◽  
Obiang-Obounou Brice ◽  
Young-Seob Lee ◽  
Hee-Sung Chae ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Ecklonia Cava ◽  
Salmonella Spp ◽  
Methicillin Resistant

Antibacterial activity of aminoderivatized chitosans against methicillin-resistant Staphylococcus aureus (MRSA)

2009 ◽  
Vol 17 (20) ◽  
pp. 7108-7112 ◽  
Author(s):  
Dae-Sung Lee ◽  
Seong-Yun Jeong ◽  
Young-Mog Kim ◽  
Myung-Suk Lee ◽  
Chang-Bum Ahn ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistant
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