scholarly journals Phytochemical screening and antibacterial activity of Garcinia kola (hackel) and Cola nitida (vent) extracts

2020 ◽  
Vol 39 (2) ◽  
pp. 379-385
Author(s):  
B.T. Afolabi ◽  
G.C. Agu ◽  
I.B. Onajobi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Aqueous Extracts ◽  
Phytochemical Screening ◽  
Garcinia Kola ◽  
Test Plants ◽  
Cola Nitida

This study was designed to evaluate the phytochemical and antibacterial properties of Garcina kola (Orogbo) and Cola nitida (Obi) extracts. Fresh seeds of Cola nitida and Garcinia kola were collected from Ago-Iwoye market, Ogun State. The ethanolic and aqueous extracts of the test plants were used against selected test organisms, Streptococcus pneumonia, Staphylococcus aureus, Salmonella typhi, Pseudomonas euriginosa and Escherichia coli. Disc diffusion method was adopted to test for susceptibility of the selected test bacteria to the extracts. Minimum inhibition concentration (MIC) and Minimum bactericidal concentration (MBC) were determined. Bioassay data were statistically analyzed using two-way ANOVA. The result of the phytochemical screening confirmed the presence of flavonoid, phenol, alkaloid, saponins, tannins and phlobatanins. The result of the antibacterial activity showed that the ethanolic extract of the test plants recorded highest antimicrobial activity against test isolates compared to aqueous extracts. The tested plant seeds of both plants posses reasonable antibacterial activity but to varied zones of inhibition, with Staphylococcus aureus isolate having the highest inhibitory zones (21.33) mm while Pseudomonas aeuruginosa had the least inhibitory zone (6.00) mm. The antibacterial activity were however found to be concentration dependent (Fvalue= 3.996, Pvalue= < 0.05). All tested organisms were found to have definite MIC and MBC activities which ranges between 125 and 1000μg/ml for MIC except for Kola nitida that has no definite MBC below 1000μg/ml. The result confirms the potential of antibacterial activity of Garcinia kola and Cola nitida extracts. Keywords: Antimicrobial, Cola nitida, Garcinia kola, Phytochemical, Plant extracts

scholarly journals THE EFFECT OF PSEUDOCEDRELA KOTSCHYI (SCHWEINF.) HARMS EXTRACTS AGAINST STAPHYLOCOCCUS AUREUS GROWTH

2021 ◽  
Vol 4 (1) ◽  
pp. 11-15
Author(s):  
Kokou Atchou ◽  
Koku Amegbo Dakey ◽  
Farida Etroubeka-Tsono ◽  
Amégninou Agban ◽  
Simplice Damintoti Karou ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Aqueous Extract ◽  
Ethanolic Extract ◽  
Clinical Strain ◽  
Aqueous Extracts ◽  
Phytochemical Screening ◽  
Bactericidal Effects ◽  
Kinetics Of ◽  
Kinetics Of Inhibition

Staphylococcus aureus is a ubiquitous bacteria that causes a serious health problem because of its multi-resistance to antibiotics. The aimed of this study was to evaluate the antibacterial activity of P. kotschyi root against S. aureus ATCC 29213 and S. aureus, a clinical strain. The phytochemical compound was sought in the extracts by standard staining tests and extractions were carried out by fractionation using solvents depletion method with increasing polarity. The method of dilution in liquid medium was used for the antibacterial tests. The results showed that the aqueous extract of the bark was effective against both S. aureus with MICs of 0.39 mg.mL-1. The MICs of ethanolic extract were 0.39 and 0.78 mg.mL-1, respectively on S. aureus ATCC 29213 and S. aureus. The steles of P. kotschyi roots were less effective. The ethanolic and aqueous extracts at concentrations = 2MICs, have been respectively bacteriostatic and bactericidal effects on tested germs. The kinetics of inhibition showed that the aqueous extract of the bark at 0.78 mg.mL-1, completely destroyed the two germs respectively in 4 h and 5 h. The action of ethanolic extract at 0.78 and 1.56 mg.mL-1 occurs late against both S. aureus at 6 h and 7 h. Phytochemical screening revealed the presence of alkaloids, tannins, flavonoids, anthracenes, glycosides, saponosides and sterols, some of that may be responsible for the observed antimicrobial activity. This study proved the antibacterial activity of P. kotschyi roots that can be exploited as an antibiotic in the treatment of S. aureus infections.

scholarly journals Screening phytochimique des extraits méthanoliques des feuilles de Combretum collinum et des racines de Anogeisus leiocarpus et effet antibactérien in vitro sur des souches de Staphylococcus aureus multirésistantes

2020 ◽  
Vol 14 (6) ◽  
pp. 2362-2372
Author(s):  
Moussa Bamba ◽  
Christel Neut ◽  
Simon Bordage ◽  
Soro Dramane ◽  
Jules Kouadio N’guessan ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Dilution Method ◽  
Plantes Médicinales ◽  
Phytochemical Screening ◽  
Methanolic Extracts ◽  
Anogeissus Leiocarpus

Anogeissus leiocarpus (DC.) Guill. & Perr. (Combretaceae) et Combretum collinum Fresen. (Combretaceae) sont deux plantes de la flore ivoirienne couramment utilisées pour traiter plusieurs pathologies telles que les affections cutanées, le paludisme et la fatigue générale. Ce travail s’inscrit dans le cadre de la valorisation de cette flore. Il vise donc à évaluer l’activité antibactérienne des extraits méthanoliques bruts des feuilles de Combretum collinum et des écorces de racines de Anogeissus leiocarpus sur la croissance "in vitro" des souches de Staphylococcus aureus résistantes à la méticilline. La méthode de diffusion en milieu gélosé (solide) a été utilisée pour évaluer la sensibilité des souches bactériennes aux extraits et à déterminer les concentrations minimales inhibitrices (CMI), Quant au screening phytochimique, il a été réalisé par une chromatographie sur couche mince (CCM). La concentration minimale inhibitrice est comprise entre 0,62 mg/ml et 1,25 mg/ml pour Anogeissus leiocarpus et est de 0,325 mg/ml pour Combretum collinum. Le screening phytochimique a révélé la présence de flavonoïdes et de tanins dans l’extrait méthanolique de Anogeissus leiocarpus alors que dans l’extrait méthanolique de Combretum collinum, ce sont des acides phénoliques qui ont été mis en évidence. Il ressort donc que ces deux plantes sont dotées d’un important pouvoir antibactérien et contiennent plusieurs composés chimiques. Elles pourraient donc constituer des voies de prospection pour la recherche de nouvelles molécules antibactériennes en réalisant une étude bio-guidée des extraits bruts et en évaluant leur cytotoxicité sur des cellules hépatiques saines.Mots clés: Antibactérienne, plantes médicinales, flore ivoirienne, extraits methanoliques English Title: Phytochemical screening of methanolic extracts from leaves of Combretum collinum and roots of Anogeisus leiocarpus and in vitro antibacterial effect on multiresistant strains of Staphylococcus aureus Both Anogeissus leiocarpus (DC.) Guill. & Perr. (Combretaceae) and Combretum collinum Fresen. (Combretaceae) are Ivorian national flora plants commonly used in treating skin disorders, malaria, and general fatigue. The current study consists of the valorization of this flora, and aims therefore at revealing antibacterial activity of the methanol crude extracts obtained from stem bark and leaves of the respective plants, against Methicillin-resistant Staphylococcus aureus strains. Agar gel diffusion method was used to assess bacteria susceptibility, and broth dilution method allowed determination of minimum inhibition concentrations (MICs). These parameters varied from 0.62 mg/ml to 1.25 mg/ml for Anogeissus leiocarpus, while they reached 0.325 mg/ml for Combretum collinum. In addition, phytochemical screening brought about flavonoids and tanins for the plant, and phenolic acids for the other one. In conclusion, these named plants contain several chemical compounds with antibacterial properties, and could be of great interest in the search of new molecular compounds provided with antibacterial activity through bio-guided experiments. Furthermore, they could best hold value by testing those methanol extracts over hepatic sane cells for cytotoxicity assessment. Keywords: antibacterial, medicinal plants, Ivorian national flora, methanolic extracts.    

In-Vitro Studies on the Sensitivity of Staphylococcus aureus to Some Ethno-Medicinal Preparations Sold around Kano, Nigeria

2011 ◽  
Vol 4 (1) ◽  
pp. 22-25 ◽  
Author(s):  
M Bashir ◽  
I Yusuf ◽  
AS Kutama
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Test Organism ◽  
Ethanolic Extract ◽  
Screening Tests ◽  
Phytochemical Screening ◽  
Herbal Preparations

Five traditional herbal preparations were sampled between May-June, 2009 in Kano. The samples were investigated for invitro antibacterial activities against clinical isolates of Staphylococcus aureus. Likewise, phytochemical screening tests were conducted to determine some of the phytochemicals present in the ethanolic and water extracts of the samples. Various concentrations of the extracts were prepared using serial doubling dilutions (5000=l/ml, 2500=g/ml, 1250=g/ml, 625=g/ml and 312.5=g/ml). All the test extracts showed slight antibacterial activity against the test organism, with ethanolic extract of sample E having the highest zone diameter of inhibition, while sample H had the lowest diameter of inhibition. The standard antibiotic disc (Gentamicin) had demonstrated the highest activity on the test organisms. The results of the Phytochemical screening revealed the presence of steroid in all the samples, tannin in samples A, C, D and E, reducing sugars in sample A, D and E respectively. The result of the minimum inhibitory concentration (MIC) was found to be above 312.5=g/ml for samples C, D and E. Keywords: Staphylococcus aureus, Herbal preparations, antibacterial activity, Phytochemical screening and minimum inhibitory concentration.

Antioxidant and Antimicrobial Activities of the Fresh aerial parts of Ammi visnaga L. from Algeria

2016 ◽  
Vol 6 (2) ◽  
pp. 70-76
Author(s):  
Soumia Keddari ◽  
Narimen Benaoum ◽  
Yasmina Mokhtaria Boufadi ◽  
Mansouria Belhocine ◽  
Ali Riazi
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Phenolic Compounds ◽  
Biological Activities ◽  
Antibacterial Properties ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
E Coli ◽  
Ammi Visnaga ◽  
Wide Range

Medicinal plants have been used for countries as cures for human diseases because they contain components of therapeutic value. Among these medi-cinal plants, Ammi visnage which have an immense reservoir of potential compounds attributed to the secondary metabolites which have the advan-tage of being of great diversity of chemical structure and have a very wide range of biological activities. The objectives of the present work were to stu-dy the antioxidant and antimicrobial activity of phenolic compounds ex-tracted from A. visnaga L. Its extraction is performed by two methods, etha-nol extraction and water extraction. The results showed that A. visnaga L.. ethanolic extract contains a mixture of phytochemical classes as polyphenol, flavonoids and revealed that this plant has high antioxidant activity (IC50 0.069 mg/ml). Regarding the antimicrobial activity results expressed by the diameter of the inhibition zones by diffusion method AWDT, the most signifi-cant inhibition was observed against to Staphylococcus aureus (12 mm) to the ethanol extract at concentration of 100mg / ml. Thus the aqueous ex-tract had a significant inhibitory activity against on the strains Staphylococ-cus aureus (8 mm), E. coli ATCC 10536 (8 mm) to a concentration of 100 mg / ml. The results for the antibacterial properties have shown that Gram-positive bacteria (Staphylococcus aureus, Listeria monocytogenes and M. luteus.) were more sensitive than gram-negative (Pseudomonas aeruginosa, E. coli ATCC 10536) against from the action of phenolic compounds of the Ammi visnaga ethanolic extract.

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL 96% DAUN MIMBA (Azadirachta indica A. Juss. ) DENGAN METODE EKSTRAKSI PERKOLASI TERHADAP PERTUMBUHAN BAKTERI

2020 ◽  
Vol 2 (1) ◽  
pp. 102
Author(s):  
Yanu Andhiarto ◽  
Rina Andayani ◽  
Nur Hidayatul Ilmiyah
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Skin Inflammation ◽  
Screening Tests ◽  
Diffusion Method ◽  
Moderate Activity ◽  
Phytochemical Screening ◽  
Strong Activity ◽  
Activity Test

Skin infections most often found in developing countries, especially in the tropics, are purulent skin inflammation (pyoderma), the main cause of Staphylococcus aureus. The increasing number of cases of bacteria that are resistant to antibacterial can encourage the extraction of sources of antibacterial drugs from natural ingredients. One of the plants that is thought to have the antibacterial activity of Staphylococcus aureus is the Neem plant. In this study an antibacterial activity test was carried out with the well diffusion method from 96% ethanol extract of Mimba leaves extracted by percolation method. Previously 96% ethanol extract from Mimba leaves had been identified by secondary metabolites by phytochemical screening and standardized extract testing. Standarization using non-specific parameters (moisture content, ash content and drying losses) found that 96% ethanol extract of Mimba leaves entered a range of standard parameters and in phytochemical screening tests obtained 96% ethanol extract of Mimba leaves containing alcaloid, saponins, tannins, steroids / terpenoids and flavonoids. The results of the study on the antibacterial activity test showed a concentration of 75% ethanol extract of 96% Mimba leaves classified as having strong activity, at a concentration of 50% and 25% classified as having moderate activity. Based on this study it can be concluded that the three concentrations of 96% ethanol extract of Mimba leaves were able to inhibit the growth of Staphylococcus aureus bacteria.

Evaluation of Antibacterial Activity of Combined Plant Extract of Pyracantha crenulata and Zanthoxylum armatum

2020 ◽  
Vol 2 (29) ◽  
pp. 32-36
Author(s):  
Semwal Amit Negi Sweta
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Moderate Activity ◽  
Aqueous Extracts ◽  
Disk Diffusion Method ◽  
Zanthoxylum Armatum ◽  
Plant Extraction

Abstract-Medicinal plants represent an essential source of drugs and have played an important role in healthcaresystem.PyracanthacrenulataandZanthoxylum armatumhave been used as traditional medicine. The main aim of the study was to find the antibacterial activity of the selected plants against bacterialspecies:E.coliandPseudomonasaeruginosa. The solvents used for plant extraction were hexane, chloroform, ethanol and aqueous. The in vitro antimicrobial activity was performed by Agar disk diffusion method. The hexane and aqueous extracts showed moderate activity whereas theethanolicextractsshowedasignificantantibacterial activity. In the study Tetracycline was used as standard. The combined ethanolic extract of both the selected plant showed the synergistic effect on the bacterial strain tested. This leads to the conclusion that the combined effect can have possible application in the development of products as antimicrobial.

scholarly journals Phytochemical and Antibacterial Properties of Ethanolic Seed Extracts of Chrysophyllum albidum (African Star Apple)

2016 ◽  
Vol 1 (1-2) ◽  
pp. 05-09 ◽  
Author(s):  
Samuel L. Oputah ◽  
Kolawole O. Ajanaku ◽  
Raphael C. Mordi ◽  
Joseph A. O. Olugbuyiro ◽  
Shade J. Olorunshola ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Antibacterial Properties ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Zone Of Inhibition ◽  
Test Organisms ◽  
Seed Extracts ◽  
African Star Apple

Phytochemical and antibacterial properties of ethanolic extract of the seeds of African Star Apple (Chrysophyllum albidum) were investigated. The phytochemical result revealed the presence of saponins, carbohydrates, flavonoids, quinones, cardiac glycosides, fatty acids and terpenoids. The antibacterial activity was studied using agar well diffusion method at different concentrations against six pathogenic bacterial strains, three Gram-positive (Staphylococcus aureus, Micrococcus varians and Bacillus cereus) and three Gram-negative (Escherichia coli, Pseudomonas aeruginosa and Proteus vulgaris). Significant inhibitory activities were exhibited by the ethanolic seed extracts for all test organisms except Bacillus cereus. Zone of inhibition of the crude ethanolic extract was correlated with that of a standard antibiotic Gentamicin, for antibacterial activity. The results indicated a notable inhibition of the bacterial growth.

scholarly journals SKRINING AKTIVITAS ANTIBAKTERI FRAKSI EKSTRAK ETANOL KERNEL BIJI BUAH LIMUS (Mangifera foetida Lour.) TERHADAP Staphylococcus aureusDAN Escherichia coli

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Vera Nurviana
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Water Fraction ◽  
Tetracycline Hcl

<p class="Default"><em>Limus (Mangifera foetida Lour.) Is one of the local Indonesian fruits of the anacardiaceae family. Limus contain secondary metabolites that have pharmacological effects, including the kernel waste of the seeds of the fruit. This study aims to determine the antibacterial activity against Staphylococcus aureus and Escherichia coli on the fraction of kernel seed of limus fruits ethanolic extract in invitro using solid diffusion method with positive control Tetracycline HCl. The extraction process was carried out by maceration method, then fractionation was obtained to obtain the fraction of n-hexane, ethyl acetate fraction and water fraction. Based on the result of the research, the fraction of n-hexane, ethyl acetate fraction, and water fraction of kernel ethanol extract limus fruit seed have antibacterial activity against Staphylococcus aureus and Escherichia coli. The highest activity was shown by fraction of ethyl acetate with minimum killing concentration (KBM) to Staphylococcus aureus was 5% with diameter equal to 4,33 ± 1.17 mm equal to 30,27 μg / mL Tetracycline HCl, and KBM value to Escherichia coli.4% (7.60 ± 0.14) mm equivalent to 26, 04 μg / mL Tetracycline HCl.<br /> <br /> <strong></strong></em></p><p class="Default"><strong><em>Keywords: </em></strong><em>Antibacterial, Staphylococcus aureus, Escherichia coli, Kernel, M. foetida.</em></p>

Sign in / Sign up

Export Citation Format

Share Document