scholarly journals Effects of Momordica Charantia Silver Nanoparticles on the expressions of Genes Associated With Lipid Metabolism and Nephrotoxicity in Streptozotocin-Induced Rats

2021 ◽  
Vol 37 (2) ◽  
pp. 126-133
Author(s):  
O.O Elekofehinti ◽  
M.O Akinjiyan
Keyword(s):  
Diabetes Mellitus ◽  
Silver Nanoparticles ◽  
Lipid Metabolism ◽  
Silver Nitrate ◽  
Nitrate Solution ◽  
Diabetic Control ◽  
Untreated Group ◽  
Rt Pcr ◽  
Hmg Coa Reductase ◽  
Coa Reductase

Hyperlipidemia and hyperglycemia have been implicated in diabetes mellitus (DM) leading to complications such as nephropathy. Medicinal plants like Mormodica charantia (MC) have been used in the treatment of DM over the years but little is known about their mechanisms of action. This study used biotechnology tools to investigate and compare the effects of M. charantia silver nanoparticles (MCSNPs) with M. charantia extract on expressions of genes linked with nephrotoxicity, lipid and glucose metabolisms using reverse-transcriptase polymerase chain reaction (RT-PCR) in streptozotocin-induced diabetic rats. The genes investigated include kidney injury molecule-1 (KIM-1), 3-hydroxyl, 3-methyl glutaryl_coA reductase (HMG-CoA reductase), peroxisome proliferator-activated receptor alpha and gamma (PPARα and PPARγ). Synthesis of MCSNPs was done using 1 mM concentration of aqueous silver nitrate solution at ratio 1:9 (v/v). Experimental rats were induced intraperitoneally with streptozotocin (65 mg/kg) and divided into six groups viz: diabetic control; normal control; silver nitrate (10 mg/kg); MCSNPs (50 mg/kg); Metformin (100 mg/kg) and M. charantia fraction (100 mg/kg). Sacrifice was done after 12 days of treatment and RT-PCR was then used to investigate gene expressions in liver and kidney tissues of the rats. The expression of HMG-CoA reductase gene was significantly upregulated (p<0.05) upon treatment with 50 mg/kg MCSNPs relative to the diabetic untreated group. M. charantia extracts and MCSNPs significantly upregulate (p<0.05) the expressions of PPAR-α and PPAR-γ compared to the diabetic control. Also, a significant (p<0.05) down-regulation of KIM-1 mRNA expression was observed in MCSNPs- treated group, relative to the diabetes untreated group. M. charantia silver nanoparticles could be a potent antidiabetic agent due to its potential to modulate genes associated with lipid metabolism and nephrotoxicity. Keywords: Medicinal plant; Diabetes Mellitus; Silver Nanoparticles; nephrotoxicity; gene expression

Controlling the Surface Plasmon Absorption of Silver Nanoparticles via Green Synthesis Using Pennisetum purpureum Leaf Extract

2018 ◽  
Vol 772 ◽  
pp. 73-77
Author(s):  
Ruelson S. Solidum ◽  
Arnold C. Alguno ◽  
Rey Capangpangan
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Silver Nitrate ◽  
Surface Plasmon ◽  
Leaf Extract ◽  
Nitrate Solution ◽  
Silver Nitrate Solution ◽  
Absorption Peak ◽  
Plasmon Absorption ◽  
Surface Plasmon Absorption

We report on the green synthesis of silver nanoparticles utilizing theP.purpureumleaf extract. Controlling the surface plasmon absorption of silver nanoparticles was achieved by regulating the amount of extract concentration and the molarity of silver nitrate solution. The surface plasmon absorption peak is found at around 430nm. The surface plasmon absorption peak have shifted to lower wavelength as the amount of extract is increased, while plasmon absorption peak shifts on a higher wavelength as the concentration of silver nitrate is increased before it stabilized at 430nm. This can be explained in terms of the available nucleation sites promoted by the plant extract as well as the available silver ions present in silver nitrate solution.

scholarly journals The Synthesis and Stability Study of Silver Nanoparticles Prepared by Using p-Aminobenzoic Acid as Reducing and Stabilizing Agent

2018 ◽  
Vol 18 (3) ◽  
pp. 421 ◽  
Author(s):  
Dian Susanthy ◽  
Sri Juari Santosa ◽  
Eko Sri Kunarti
Keyword(s):  
Silver Nanoparticles ◽  
Reaction Time ◽  
Silver Nitrate ◽  
Tap Water ◽  
Nitrate Solution ◽  
Silver Nitrate Solution ◽  
Stability Study ◽  
Synthesis Process ◽  
Aminobenzoic Acid ◽  
Stabilizing Agent

A study to examine the performance of p-aminobenzoic acid as both reducing agent for silver nitrate to silver nanoparticles (AgNPs) and stabilizing agent for the formed AgNPs has been done. The synthesis of AgNPs was performed by mixing silver nitrate solution as precursor with p-aminobenzoic acid solution and heating it in a boiling water bath. After the solution turned to yellow, the reaction stopped by cooling it in tap water. The formed AgNPs were analyzed by using UV-Vis spectrophotometry to evaluate their SPR absorption in wavelength range of 400–500 nm. The synthesis process was highly depend on the pH, reaction time, and mole ratios of the reactants. The synthesis only occur in pH 11 and at reaction time 30 min, the particle size of the formed AgNPs was 12 ± 7 nm. Longer reaction time increased the reducing performance of p-aminobenzoic acid in AgNPs synthesis but decreased its stabilizing performance. The increase of silver nitrate amount relative to p-aminobenzoic acid in the synthesis increased the reducing and stabilizing performance of p-aminobenzoic acid and the optimum mole ratio between AgNO3 and p-aminobenzoic acid was 5:100 (AgNO3 to p-aminobenzoic acid).

2.P.62 Effect of eicosapentaenoic acid in combination with HMG-CoA reductase inhibitor on lipid metabolism

1997 ◽  
Vol 134 (1-2) ◽  
pp. 129
Author(s):  
H. Mataki ◽  
K. Watuji ◽  
S. Miwa ◽  
K. Matsushita ◽  
T. Miki ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Eicosapentaenoic Acid ◽  
Reductase Inhibitor ◽  
Hmg Coa Reductase ◽  
Hmg Coa Reductase Inhibitor ◽  
Coa Reductase

Pretreated Lignocellulosic Waste Mediated Biosynthesis of Silver Nanoparticles Under Room Temperature

2016 ◽  
Vol 15 (05n06) ◽  
pp. 1660001 ◽  
Author(s):  
V. P. Manjamadha ◽  
Karuppan Muthukumar
Keyword(s):  
Silver Nanoparticles ◽  
Silver Nitrate ◽  
Room Temperature ◽  
Nitrate Solution ◽  
Silver Ions ◽  
Silver Nitrate Solution ◽  
Morphological Characterization ◽  
Alternative Source ◽  
X Ray ◽  
Antibacterial Performance

The current work elucidates the utilization of biowaste as a valuable reducing agent for the synthesis of silver nanoparticles. In this study, the wastewater generated during the alkaline pretreatment of lignocellulosic wastes (APLW) was used as a bioreductant to reduce silver nitrate under room temperature. Synthesis of stable silver nanoparticles (AgNPs) was achieved rapidly on addition of APLW into the silver nitrate solution (1[Formula: see text]mM). The morphological characterization of AgNPs was performed using field emission scanning electron microscopy (FESEM). The micrograph clearly depicted the presence of spherical AgNPs. The presence of elemental silver along with biomoilties was determined using energy dispersive X-ray spectroscopy (EDAX) analysis. The X-ray diffraction (XRD) study proved the crystalline form of stable AgNPs. The AgNPs exhibited excellent antibacterial performance against Gram negative organism. The immediate bioreduction of silver ions using APLW was well illustrated in the present study. Thus, APLW serve as an alternative source for reducing agents instead of utilizing valuable medicinal plants for nanoparticles synthesis.

Improved endothelial function and reduced platelet activation by chronic HMG-CoA-reductase inhibition with rosuvastatin in rats with streptozotocin-induced diabetes mellitus

2007 ◽  
Vol 73 (9) ◽  
pp. 1367-1375 ◽  
Author(s):  
Andreas Schäfer ◽  
Daniela Fraccarollo ◽  
Christian Vogt ◽  
Ulrike Flierl ◽  
Melinda Hemberger ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Endothelial Function ◽  
Platelet Activation ◽  
Hmg Coa Reductase ◽  
Streptozotocin Induced Diabetes ◽  
Coa Reductase

scholarly journals Mechanisms underlying recoupling of eNOS by HMG-CoA reductase inhibition in a rat model of streptozotocin-induced diabetes mellitus

2008 ◽  
Vol 198 (1) ◽  
pp. 65-76 ◽  
Author(s):  
Philip Wenzel ◽  
Andreas Daiber ◽  
Matthias Oelze ◽  
Moritz Brandt ◽  
Ellen Closs ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Rat Model ◽  
Hmg Coa Reductase ◽  
Streptozotocin Induced Diabetes ◽  
Coa Reductase

scholarly journals Green Silver Nanoparticles Embedded in Cellulosic Network for Fresh Food Packaging

2021 ◽  
Author(s):  
S. Chaitanya Kumari ◽  
P. Naga Padma ◽  
K. Anuradha
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Silver Nitrate ◽  
Shelf Life ◽  
Food Packaging ◽  
Nitrate Solution ◽  
Packaging Material ◽  
Packaging Materials ◽  
Coated Paper ◽  
Fresh Food

The demand for increasing the shelf life of fresh food as well as the need for protecting the food against foodborne infections warrant the demand for increasing the shelf life of fresh food. The incorporation of nanoparticles into the packaging material can enhance the preservation of perishable foods. Silver nanoparticles (AgNPs), in particular, have antibacterial, anti-mold, anti-yeast, and anti-viral activities can be embedded into the biodegradable packaging materials for this purpose. This study focuses on antimicrobial packaging materials for food by mixing the extracts of different plants with silver nitrate and depositing this mixture as a layer on the blotting papers, which are thick sheets of paper made of cellulose. Because the blotting papers are highly absorbent and porous, silver nitrate solution along with the plant extracts can be easily applied and allowed for in situ synthesis of AgNPs. Subsequently, these papers were analyzed and characterized using scanning electron microscopy, transmission electron microscopy, atomic absorption spectroscopy, and energy dispersive X-ray analysis. The coated paper exhibited good antibacterial activity against Escherichia coli and Staphylococcus aureus. Furthermore, the coated paper when used as a packaging material for tomatoes and coriander leaf, the shelf life was extended to about 30 days and 15 days respectively. The prepared cost-effective silver packing material can be used in food packaging for various perishable foods.

Fast colourimetric detection of H2O2 by biogenic silver nanoparticles synthesised using Benincasa hispida fruit extract

2016 ◽  
Vol 5 (2) ◽  
Author(s):  
Kaushik Roy ◽  
Chandan K. Sarkar ◽  
Chandan K. Ghosh
Keyword(s):  
Silver Nanoparticles ◽  
Silver Nitrate ◽  
Nitrate Solution ◽  
Silver Nitrate Solution ◽  
Single Step ◽  
Fruit Extract ◽  
Biogenic Silver Nanoparticles ◽  
Benincasa Hispida

AbstractThis study reports a single-step, fast and eco-friendly procedure for preparing silver nanoparticles from silver nitrate solution using fruit extract of

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