scholarly journals Antioxidant Potential of Crude Powder, Methanol and Aqueous Extracts of Fonio Millet (Digitaria exilis) Grains

2021 ◽  
Vol 38 (1) ◽  
pp. 91-97
Author(s):  
G.O. Omaji ◽  
M. Osibemhe ◽  
B.O. Orji ◽  
L.E. Ilouno ◽  
B.O. Abdulrahman ◽  
...  

This study evaluated the antioxidant potential along with total phenolic content, total flavonoid content of the crude powder, methanol and aqueous extracts of Digitaria exilis grains. The antioxidant activities of the crude powder and extracts were determined by measuring the reducing ability and hydrogen peroxide scavenging activity. The results showed that methanol extracts exhibited the highest total phenolic content (57.96 ± 6.84 mg gallic acid equivalence/g dried weight) and total flavonoid content (38.75 ± 9.76 mg quercetin equivalence/g dried weight) compared to the crude powder and aqueous extracts. A concentration- dependent increase in the reducing ability and hydrogen peroxide scavenging activity was observed in all the samples. The results were comparable to ascorbic acid, the standard antioxidant used. These results indicate that Digitaria exilis grains have antioxidant activity and may account for the use of the grains in traditional systems of medicine.

2021 ◽  
Vol 913 (1) ◽  
pp. 012042
Author(s):  
N Ardiana ◽  
A S Abidin ◽  
B T K Ilhami ◽  
A L Sunarwidhi ◽  
S Widyastuti ◽  
...  

Abstract Seaweed is one of the marine-biota that is widely known for its uses, one of them is in the food functional. Functional food is a program that promotes a healthy diet so that it can prevent a disease when consuming food. With functional food, daily eating patterns can be maintained and also the content of the food consumed daily has a good effect on the body. It is known that in seaweed, especially brown algae (S. crassifolium), it contains antioxidants and other compounds that are good for the health. In this study, phytochemical content and proximate composition was evaluated in S. crassifolium. Including total flavonoid content and total phenolic content, antioxidant capacity both of DPPH and ABTS assay used three type of solvents such as Ethanol, n-Hexana, and ethyl acetate for comparable the best solvents. Also, proximate analysis to determine the content of moisture content, crude fat content, crude protein content, and carbohydrates content. The results showed that n-Hexana and ethyl acetate solvents were significantly than ethanol. Which mean both of them had highest values of total flavonoid content (TFC), total phenolic content (TPC), and antioxidant activity of DPPH radical scavenging activity and ABTS radical scavenging activity. The results of chemical composition in proximate compounds showed that S. crassifolium was suitable to be candidate of functional food.


Foods ◽  
2018 ◽  
Vol 7 (9) ◽  
pp. 143 ◽  
Author(s):  
Pierre Sombié ◽  
Moussa Compaoré ◽  
Ahmed Coulibaly ◽  
Jeremy Ouédraogo ◽  
Jean-Baptiste Tignégré ◽  
...  

Antioxidant compounds of dietary plants have been widely studied because of their bioactive properties. The objective of this research study was to analyse the health enhancing attributes of 31 cowpeas varieties from Burkina Faso. Significant variations were observed in the phenolic content as well as the antioxidant and anti-lipid peroxidation activities amongst the cowpea varieties. Pearson correlation coefficient analysis showed that the ferric reducing ability (r = 0.954) and anti-lipid peroxidation (r = 0.616) were positively correlated with the total phenolic content. A significant relationship between cowpea ferric reducing ability and anti-lipid peroxidation (r = 0.64) was also revealed. However, nitric oxide scavenging potential was found not to be related to its total phenolic and total flavonoid content. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radicals scavenging potentials were not correlated with the total flavonoid content. The pigmented seeds of cowpea varieties possess higher total phenolic, total flavonoid content, ferric reduction ability, and anti-lipid peroxidation activities than the colourless ones. The results obtained from this study suggest that Burkina Faso cowpea cultivars are rich in phenolic compounds and have significant antioxidant and anti-lipid peroxidation activities. Consumption of the cowpea, particularly of coloured cowpea seed varieties, should be beneficial for chronic human diseases prevention.


Author(s):  
Jigisha Anand ◽  
Shanu Chaudhary ◽  
Nishant Rai

 Objective: The present study aims at screening phytochemicals present in Lantana camara leaves and flower extracts.Methods: Methanolic extracts of leaves and flower of L. camara were prepared by the solvent extraction method. The preliminary qualitative photochemical analysis of leaves and flower extract was done following standard procedures. For quantitative phytochemical screening, total phenolic content (TPC), total flavonoid content (TFC), and free radical scavenging activity of L. camara was estimated.Results: The preliminary qualitative analysis showed the presence of flavonoids, phenols, terpenoids, anthocyanins, carbohydrates, and proteins in the methanolic extracts of Lantana leaves and flower, while saponins, glycosides, and alkaloids were not detected in both the extracts. Quantitative assays were done for determination of TPC, TFC, and free radical scavenging activity of L. camara. The methanolic extract of Lantana flowers depicted highest TFC (15.76±0.005 mg of hydrated catechin equivalent/ml of sample) and TPC (17±0.005 mg of Gallic acid equivalents equivalent/ml of sample), while both leaves and flower extracts revealed extortionately high 2, 2-diphenyl-1-picrylhydrazyl free radical scavenging activity.Conclusion: The present work depicted that L. camara leaves and flowers are rich in antioxidant activity and have a promising application as nutraceuticals for herbal drug formulations.


Author(s):  
Aruna Kumari ◽  
Sharma Ra

Objective: Estimation of total phenol, flavonoid and antioxidant activity of various plant parts (leaves, stem, root, flower and fruit) of Ailanthus excelsa Roxb. methanolic extracts.Methods: Different plant parts were extracted with methanol, then the total phenol content was calculated by using Folin–Ciocalteu reagent, total flavonoid content was estimated by using Aluminum Chloride Colorimetric Method and the antioxidant activity was measured by DPPH (1,1-diphenyl-2-picrylhydrazyl radical) assay.Results: The highest total phenolic content (48.38 mg GAE/gdw) was observed in flower and lowest phenolic content in root (28.56 mg GAE/gdw). The highest total flavonoidal content (21.5 mg QE/gdw) was found in leaf and lowest in root (1.11 mg QE/gdw). The highest radical scavenging activity was found in flower extracts with the IC50 value of 36.85 µg/ml and the lowest scavenging activity was observed in root extract that was found to be 1493.46µg/ml.Conclusion: According to the results of present investigation the plant showed significant antioxidant activity that can be used for medical purpose for the treatment of various diseases.Keywords: Antioxidant activity, Ailanthus excelsa Roxb., Total phenolic content, Total flavonoid content, DPPH (2, 2- diphenyl- 1- picryl hydrazyl), Scavenging activity.                                                               


Author(s):  
Mentham Ramesh ◽  
Chandu Babu Rao

The current investigation is intended to evaluate the content of phytochemical constituents and antioxidant potential of hydroalcoholic extracts of stem and root of Grewia serrulata DC (HAESGS & HAERGS) and leaf and bark of Grewia Nervosa (Lour.) panigrahi (HAELGN & HAEBGN). Initially, all the extracts at different concentrations were estimated for their total phenolic content and total flavonoid content. The study was further extended for their antioxidant potential evaluation using various in vitro methods such as 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical scavenging assays. The total phenolic content (mg gallic acid equivalent per gram of extract) was high in HAELGN (170.82±0.19) and HAERGS (123.00±0.48) than HAESGS (111.2±0.26) and HAEBGN (119.60±0.23). The total flavonoid content (mg quercetin equivalent per gram) is greater in HAERGS (71.24±0.50) and HAESGS (65.68±0.27) than HAELGN (55.82±0.35) and HAEBGN (62.38±0.45). The IC50 values (µg/ml) of different plant extracts inferred that DPPH radical scavenging activity is greater in HAELGN (42.91±0.88) and HAEBGN (53.87±0.35) than HAESGS (126.73±1.20) and HAERGS (88.87±1.25). However, hydroxyl and superoxide radical scavenging activity is more in HAERGS (135.41±1.19 & 88.00±1.42) and HAELGN (172.28±1.91 & 108.163±1.09) than HAESGS (237.3±1.65 & 110.074±0.87) and HAEBGN (204.7±1.04 & 125.54±1.07). The results of present comprehensive analysis demonstrated that both the plants Grewia serrulata DC and Grewia Nervosa (Lour.) panigrahi possess high phenolic, flavonoid contents and potential antioxidant activity, and could be used as a valid source of natural antioxidants and might be utilized for pharmacological screening of various therapeutic activities. Keywords: Grewia serrulata; Grewia Nervosa; Total Phenolic content; Total flavonoid content; Antioxidant potential


2018 ◽  
Vol 17 (4) ◽  
pp. 337-348
Author(s):  
Bai Zhouya ◽  
Huang Xiaojun ◽  
Meng Jinxia ◽  
Kan Lijiao ◽  
Nie Shaoping

Distribution of phenolic compounds and antioxidant activities was surveyed in 24 varieties of Chinese cowpea from 4 provinces. Identity of phytochemicals were determined by UPLC-ESIQTOF-MS/MS and quantified by HPLC-ESI-QqQ-MS/MS. Seven phenolic acids, 16 flavonoids and 9 other compounds were identified and several of these were quantified. Quercetin-3-glucoside and kaempferol-glucoside were the major phenolic compounds. In addition, our study indicated that OJYDH cultivar from Jiangxi province had the highest total phenolic content, total flavonoid content and stronger antioxidant activities than other cowpea cultivars. This study made a comprehensive investigation on antioxidants from cowpea and provided the useful data to support its function.


2021 ◽  
Vol 11 (11) ◽  
pp. 4745
Author(s):  
Toncho Dinev ◽  
Milena Tzanova ◽  
Katya Velichkova ◽  
Diyana Dermendzhieva ◽  
Georgi Beev

Plant extracts are an important alternative to antibiotics, which are ever more restricted because of their developing microbial resistance and some adverse effects that have been observed following frequent application. The aim of the present study was to determine the antifungal and antioxidant activity of the methanolic extracts of Acorus calamus, Chlorella vulgaris, Lemna minuta and Scenedesmus dimorphus. The antifungal activity of the extracts against strains of Aspergillus flavus, Aspergillus parasiticus, Aspergillus ochraceus, Aspergillus niger, Aspergillus carbonarius, Fusarium graminearum, Fusarium oxysporum, Penicillium chrysogenum and Alternaria alternata was evaluated via the agar well diffusion method. The antioxidant activity of the extracts was measured through the determination of three parameters—total phenolic content, total flavonoid content and radical scavenging potential (determined through UV/Vis analysis). A. calamus extracts had the highest antimicrobial activity against eight fungal strains, followed by the C. vulgaris, L. minuta and S. dimorphus extracts, which were inhibitory against two to three strains. Among the extracts from the species studied, the extract from S. dimorphus showed the highest antioxidant potential, as determined via the DPPH (1,1’-diphenyl-2-picrylhydrazil-radical) method. This correlated to its high total phenolic and flavonoid content. From A. calamus and L. minuta, methanolic extracts were obtained that exhibited similar values of the aforementioned parameters, followed by C. vulgaris extracts, which showed the lowest antioxidant activity. Based on the Pearson correlation coefficients, the impacts of the total phenolic content and the total flavonoid content on radical scavenging capacity are similar, and flavonoids were a significant part of the total phenolic compounds extracted from the plant materials studied.


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