Studies on the tolerance of soybean (<i>Glycine max,/i> (l.) Merrill) to Bean Pod Mottle Virus (BPMV) (genus <i>Comovirus</i>) in humid tropical zone of Nigeria

Nas áreas produtoras de feijão (Phaseolus vulgaris) do Estado do Paraná observa-se anualmente a ocorrência do vírus do mosaico em desenho do feijoeiro (Bean rugose mosaic virus, BRMV), principalmente em infecções mistas com o vírus do mosaico dourado do feijoeiro (Bean golden mosaic virus, BGMV), acarretando maior severidade de sintomas e causando perdas na produção. Recentemente constatou-se a presença do vírus do mosaico severo do caupi (Cowpea severe mosaic virus, CPSMV) associado a sintomas de queima do broto em plantações de soja (Glycine max) na região de Londrina, sendo este um fato novo no Estado. Neste trabalho, parte do RNA2 de dois comovirus isolados de soja no Paraná foram clonados e sequenciados, sendo 600 pares de bases (pb) do BRMV-PR e 594 pb do CPSMV-PR. Posteriormente, as seqüências correspondentes de aminoácidos foram comparadas com seis seqüências de vírus do gênero Comovirus depositadas no GenBank. Com base nestes dados observou-se que o segmento do RNA2 do isolado CPSMV-PR apresentou homologia de 85% com parte de uma seqüência já conhecida do RNA2 do CPSMV, enquanto que o segmento do RNA2 do isolado BRMV-PR apresentou homologia de 39% com o CPSMV, e de 44% com o Bean pod mottle virus (BPMV). Este trabalho apresenta pela primeira vez dados de sequenciamento parcial do BRMV, o que poderá contribuir para sua completa caracterização molecular e para o estabelecimento de estratégias para obtenção de plantas resistentes ao vírus.

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Virus-Induced Gene Silencing and Transient Gene Expression in Soybean (Glycine max) Using Bean Pod Mottle Virus Infectious Clones

Current Protocols in Plant Biology ◽

10.1002/cppb.20012 ◽

2016 ◽

Vol 1 (2) ◽

pp. 263-283 ◽

Cited By ~ 5

Author(s):

Steven A. Whitham ◽

Lori M. Lincoln ◽

R. V. Chowda-Reddy ◽

Jaime D. Dittman ◽

Jamie A. O'Rourke ◽

...

Keyword(s):

Gene Expression ◽

Glycine Max ◽

Gene Silencing ◽

Transient Gene Expression ◽

Mottle Virus ◽

Virus Induced Gene Silencing ◽

Bean Pod Mottle Virus ◽

Infectious Clones

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Bean pod mottle virus. [Distribution map].

Distribution Maps of Plant Diseases ◽

10.1079/dmpd/20063191813 ◽

2006 ◽

Author(s):

Keyword(s):

North Carolina ◽

South Carolina ◽

Glycine Max ◽

South Dakota ◽

Mottle Virus ◽

Distribution Map ◽

Cerotoma Trifurcata ◽

Bean Pod Mottle Virus ◽

Virus Distribution ◽

New Distribution

Abstract A new distribution map is provided for Bean pod mottle virus. Comoviridae: Comovirus. Hosts: common bean (Phaseolus vulgaris) and soyabean (Glycine max). Information is given on the geographical distribution in Asia (Iran), North America (Canada, Ontario, USA, Alabama, Arkansas, Illinois, Indiana, Iowa, Kansas, Kentucky, Louisiana, Mississippi, Nebraska, North Carolina, Ohio, South Carolina, South Dakota, Tennessee, Virginia, Wisconsin), South America (Brazil, Ecuador, Peru). It is vectored by bean leaf bettle, Cerotoma trifurcata (Coleoptera: Chrysomelidae).

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Microsatellite diversity of soybean genotypes differing in bean pod mottle virus leaf symptom

Canadian Journal of Plant Science ◽

10.4141/cjps08046 ◽

2009 ◽

Vol 89 (2) ◽

pp. 359-367 ◽

Cited By ~ 1

Author(s):

M. A. Rouf Mian ◽

Sung-Taeg Kang ◽

Margaret G. Redinbaugh

Keyword(s):

Genetic Diversity ◽

Glycine Max ◽

Seed Quality ◽

Mottle Virus ◽

Bean Pod Mottle Virus ◽

Plant Introductions ◽

Soybean Genotypes ◽

The Usa ◽

Genetic Diversity Study ◽

Leaf Symptoms

Bean pod mottle virus (BPMV) is a threat to soybean in most soybean [Glycine max (L.) Merr.] growing states of the USA. In the absence of complete resistance of soybean to BPMV, partial resistance can play an important role in reducing seed-yield and seed-quality losses from this virus. The objectives of this study were: (i) to evaluate plant introductions (PIs) for expression of leaf symptoms under greenhouse conditions following inoculation with BPMV, and (ii) to determine the genetic diversity among soybean genotypes with differences in leaf symptoms of BPMV. Large significant (P ≤ 0.001) differences in expression of BPMV leaf symptoms among genotypes in the greenhouse were detected. A genetic diversity study was conducted on 48 soybean genotypes differing in leaf symptoms of BPMV using 271 alleles amplified by 84 microsatellite primer pairs. The average polymorphism information content for the microsatellites was 0.53 and the average number of alleles per microsatellite was 3.23. The soybean genotypes clustered into distinct groups based on their country of origin and/or their BPMV leaf symptoms. Based on the microsatellite data, the genotypes were clustered into seven groups. Groups 1, 2 and 4 included genotypes from China only, group 3 included genotypes from the USA only and group 7 included genotypes from Japan only. Group 1 consisted of genotypes with low leaf symptoms of BPMV while group 6 was formed of genotypes with high leaf symptoms of BPMV. Key words: Bean pod mottle virus, genetic diversity, Glycine max, simple sequence repeat

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R-BPMV-Mediated Resistance to Bean pod mottle virus in Phaseolus vulgaris L. Is Heat-Stable but Elevated Temperatures Boost Viral Infection in Susceptible Genotypes

Viruses ◽

10.3390/v13071239 ◽

2021 ◽

Vol 13 (7) ◽

pp. 1239

Author(s):

Chouaïb Meziadi ◽

Julie Lintz ◽

Masoud Naderpour ◽

Charlotte Gautier ◽

Sophie Blanchet ◽

...

Keyword(s):

Elevated Temperature ◽

Phaseolus Vulgaris ◽

Elevated Temperatures ◽

Heat Waves ◽

Dominant Gene ◽

Atmospheric Temperature ◽

Mottle Virus ◽

Bean Pod Mottle Virus ◽

Heat Stable ◽

The Impact

In the context of climate change, elevated temperature is a major concern due to the impact on plant–pathogen interactions. Although atmospheric temperature is predicted to increase in the next century, heat waves during summer seasons have already become a current problem. Elevated temperatures strongly influence plant–virus interactions, the most drastic effect being a breakdown of plant viral resistance conferred by some major resistance genes. In this work, we focused on the R-BPMV gene, a major resistance gene against Bean pod mottle virus in Phaseolus vulgaris. We inoculated different BPMV constructs in order to study the behavior of the R-BPMV-mediated resistance at normal (20 °C) and elevated temperatures (constant 25, 30, and 35 °C). Our results show that R-BPMV mediates a temperature-dependent phenotype of resistance from hypersensitive reaction at 20 °C to chlorotic lesions at 35 °C in the resistant genotype BAT93. BPMV is detected in inoculated leaves but not in systemic ones, suggesting that the resistance remains heat-stable up to 35 °C. R-BPMV segregates as an incompletely dominant gene in an F2 population. We also investigated the impact of elevated temperature on BPMV infection in susceptible genotypes, and our results reveal that elevated temperatures boost BPMV infection both locally and systemically in susceptible genotypes.

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