scholarly journals In vitro evaluation of inhibitory effect of Phoenix dactylifera bark extract on rat lipid peroxidation and blood hemolysis

2016 ◽  
Vol 15 (8) ◽  
pp. 1707
Author(s):  
Amir Siahpoosh ◽  
Inas Soleimani
Keyword(s):  
Lipid Peroxidation ◽  
Phoenix Dactylifera ◽  
Inhibitory Effect ◽  
Bark Extract

scholarly journals Inhibitory Effect of Quassia amara Linn. Crude Bark Extract on Entamoeba histolytica in vitro

2014 ◽  
Vol 48 (4) ◽  
Author(s):  
Jayson C. Panganiban ◽  
Annarose L. Patupat ◽  
Jose Antonio T. Paulino ◽  
Grace G. Penserga ◽  
Mar Aristeo G. Poncio ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Inhibitory Effect ◽  
Bark Extract ◽  
Quassia Amara

...

scholarly journals Inhibitory Effect of the Stem Bark Extracts of Albizia chevalieri Harms on the Activity of Alpha-glucosidase Obtained from Aspergillus niger

2019 ◽  
pp. 1-10
Author(s):  
Abdulhafiz Damilola Oso ◽  
Idris Bello ◽  
Yusuf Sa’idu ◽  
Onu Andrew
Keyword(s):  
Ethyl Acetate ◽  
Competitive Inhibition ◽  
Stem Bark ◽  
Inhibitory Effect ◽  
Acetate Buffer ◽  
Bark Extract ◽  
Methanol Extracts ◽  
Glucosidase Activity ◽  
Alpha Glucosidase

The aim of the current study is to evaluate the inhibition of α-glucosidase activity by stem bark extract of Albizia chevalieri. The activity of alpha glucosidase was assayed in vitro using 50 mM acetate buffer pH 6.0 (prepared from acetic acid and sodium acetate) and various concentration of maltose (0.5 mM to 10 mM). Five test tubes, labeled TA – TE, each containing 1.5 ml of acetate buffer, 0.5 ml of alpha glucosidase and 0.5 ml of a known concentration of plant extract and control tubes (CA – CE) were assessed for Alpha glucosidase activity. The results showed that hexane, ethyl acetate and methanol extracts inhibited α-glucosidase activity. The results further indicated that the extracts act by competitive inhibition with inhibition constant of 232 mg/ml, 157 mg/ml and 67 mg/ml for hexane, ethyl acetate and methanol extracts, respectively. The value for the inhibition constants shows that there is a strong binding of the enzyme to the inhibitor as the polarity of solvent increases. The inhibitory activity of Albizia chevalieri may be due one or more of the phytochemicals present in the extracts.

scholarly journals INHIBITORY EFFECT OF A STANDARDIZED HYDROETHANOLIC EXTRACT OF TERMINALIA ARJUNA BARK ON ALPHA-AMYLASE ENZYMEINHIBITORY EFFECT OF A STANDARDIZED HYDROETHANOLIC EXTRACT OF TERMINALIA ARJUNA BARK ON ALPHA-AMYLASE ENZYME

2018 ◽  
Vol 11 (4) ◽  
pp. 366 ◽  
Author(s):  
Sushant A Shengule ◽  
Sanjay Mishra ◽  
Shweta Bodhale
Keyword(s):  
Inhibitory Activity ◽  
High Performance ◽  
Soxhlet Extraction ◽  
Inhibitory Effect ◽  
Alpha Amylase ◽  
Antidiabetic Activity ◽  
Bark Extract ◽  
Terminalia Arjuna ◽  
Hydroethanolic Extract

 Objective: The present study was initiated to screen the hydroethanolic bark extract for α-amylase inhibitory activity and standardization of the Terminalia arjuna for polyphenolic phytochemicals using high-performance liquid chromatography-photo diode array (HPLC-PDA) method.Methods: The T. arjuna bark sample was extracted with ethanol: water (70:30 v/v) using Soxhlet extraction. A Dionex P680 HPLC system was used to acquire chromatograms. The screening of extract of T. arjuna bark has performed for in vitro α-amylase inhibitory assay. Each experiment was repeated 3 times. All values were expressed mean ± standard deviation.Results: The content of arjunetin, arjungenin, gallic acid, ellagic acid, and quercetin was 0.47, 8.22, 2.443, 7.901, and 3.20 mg/g, respectively, in a hydroethanolic extract of T. arjuna. The hydroethanolic extract of T. arjuna bark and acarbose has shown an inhibitory activity with an IC50 value 145.90 and 62.35 μg/mL, respectively.Conclusion: The hydroethanolic extract T. arjuna bark demonstrates α-amylase inhibitory activity due to a synergistic effect of the phytochemical constituents present in it. This study suggests that one of the mechanisms of this plant for antidiabetic activity is through the inhibition of α-amylase enzyme.

Scavenging of Reactive Oxygen Species by a Urinary Preparation

2000 ◽  
Vol 28 (02) ◽  
pp. 251-258 ◽  
Author(s):  
Wen-Chuan Lin ◽  
Tung-Yuan Lai ◽  
Yuen-Wern Wu
Keyword(s):  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Anion Radical ◽  
Ex Vivo ◽  
Antioxidant Properties ◽  
Order Rate Constant ◽  
Inhibitory Effect ◽  
Oxygen Species ◽  
Oxide Anion Radical

In the present paper, the antioxidant properties of a preparation of human urine (PHU) were evaluated by studying the ability of this drug to react with relevant biological oxidants such as super-oxide anion radical ( O 2·-) and hydroxyl radical ( OH ·). In addition, its effect on lipid peroxidation was investigated in vitro and ex vivo. PHU is not a good scavenger of O2·-. However, it react rapidly with OH· radicals with a second-order rate constant of 2.8 × 109/M/sec. The studies on rat brain homogenates showed that PHU had an inhibitory effect, which was dependent on its concentration and the magnitude of lipid peroxidation. Ex vivo studies also showed that oral administration of PHU increased the antioxidant capacity of plasma from rats. The ability of PHU to scavenge free radicals suggests that this drug may be potentially useful in counteracting free radical-mediated diseases.

In vitro Antioxidant and Antiproliferative Activity of the Stem Extracts from Graptopetalum paraguayense

2008 ◽  
Vol 36 (02) ◽  
pp. 369-383 ◽  
Author(s):  
Shu-Ju Chen ◽  
Jing-Gung Chung ◽  
Yun-Chin Chung ◽  
Su-Tze Chou
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Inhibitory Effect ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
Inhibition Concentration ◽  
G1 Phase Arrest ◽  
Antioxidant Reactions

This study was aimed to evaluate the antioxidant abilities of water (SGWE), 50% ethanolic (SGE50) and 95% ethanolic (SGE95) extracts from the stem of Graptopetalum paraguayense, and the extract with the highest antioxidant activity was assayed for its inhibitory effect on proliferation of human hepatoma (Hep G2) cell line. Antioxidant abilities of extracts were assessed their radical-scavenging abilities and effects on Fe /ascorbate-induced lipid peroxidation in a liposome model system. The results of this study showed that antioxidant activities were increased with the increase of the extracts concentrations, and the activities correlated with both the total phenol and anthocyanin contents. A comparison of the 50% inhibition concentration (IC50) values of different antioxidant reactions revealed that SGWE was the more effective at scavenging superoxide anion radical and preventing lipid peroxidation than SGE50 and SGE95 ( p < 0.05). The flow cytometry results indicated that SGWE lowered cell viability, and induced G1 phase arrest and apoptosis in Hep G2 cells. These results demonstrated the antioxidatant and anti-hepatoma potential of stem of Graptopetalum paraguayense.

Evaluation of the Proposed Inhibitory Effect of the Aqueous Stem-Bark Extract of Ficus exasperata on Uterine Preparations in vitro

2008 ◽  
Vol 5 (1) ◽  
pp. 94-97 ◽  
Author(s):  
E.E. Bafor ◽  
M. Nwiko ◽  
E.K.I. Omogbai ◽  
R.I. Ozolua ◽  
Z.A.M. Nworgu
Keyword(s):  
Stem Bark ◽  
Inhibitory Effect ◽  
Bark Extract ◽  
Stem Bark Extract

scholarly journals Phytic Acid Inhibits Lipid PeroxidationIn Vitro

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Alicja Zajdel ◽  
Adam Wilczok ◽  
Ludmiła Węglarz ◽  
Zofia Dzierżewicz
Keyword(s):  
Lipid Peroxidation ◽  
Linoleic Acid ◽  
Phytic Acid ◽  
Inhibitory Effect ◽  
Acid Oxidation ◽  
Colonic Epithelial Cells ◽  
Linoleic Acid Oxidation ◽  
Small Intestinal

Phytic acid (PA) has been recognized as a potent antioxidant and inhibitor of iron-catalyzed hydroxyl radical formation underin vitroandin vivoconditions. Therefore, the aim of the present study was to investigate, with the use of HPLC/MS/MS, whether PA is capable of inhibiting linoleic acid autoxidation and Fe(II)/ascorbate-induced peroxidation, as well as Fe(II)/ascorbate-induced lipid peroxidation in human colonic epithelial cells. PA at 100 μM and 500 μM effectively inhibited the decay of linoleic acid, both in the absence and presence of Fe(II)/ascorbate. The observed inhibitory effect of PA on Fe(II)/ascorbate-induced lipid peroxidation was lower (10–20%) compared to that of autoxidation. PA did not change linoleic acid hydroperoxides concentration levels after 24 hours of Fe(II)/ascorbate-induced peroxidation. In the absence of Fe(II)/ascorbate, PA at 100 μM and 500 μM significantly suppressed decomposition of linoleic acid hydroperoxides. Moreover, PA at the tested nontoxic concentrations (100 μM and 500 μM) significantly decreased 4-hydroxyalkenal levels in Caco-2 cells which structurally and functionally resemble the small intestinal epithelium. It is concluded that PA inhibits linoleic acid oxidation and reduces the formation of 4-hydroxyalkenals. Acting as an antioxidant it may help to prevent intestinal diseases induced by oxygen radicals and lipid peroxidation products.

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