Antioxidant and inhibitory effect of red ginger (Zingiber officinale var. Rubra) and white ginger (Zingiber officinale Roscoe) on Fe2+ induced lipid peroxidation in rat brain in vitro

Infertility and Neurodegerative diseases have been linked to oxidative stress arising from peroxidation of membrane biomolecules and high levels of iron have been reported to play an important role. The present study sought to determine the antioxidant activity and protective ability of n-butanol extract of Limonium bonduelli on lipid peroxidation induced by FeSO4 in rat brain and testes homogenates in vitro. n-butanol extract of the aerial parts (leaves and flowers) was prepared, and the ability of the extract to inhibit FeSO4 induced lipid peroxidation in isolated rat brain and testes was determined using spectrophotometric method. The study revealed that the extract inhibited malondialdehyde (MDA) production in FeSO4 induced lipid peroxidation in the brain and testes in a dose-dependent manner and the highest percentage of the inhibition was 89.80% similar to vitamin C in the same concentration (100 µg/mL) in brain and 82.33% in testes (200 µg/mL). Limonium bonduelli extract demonstrated important anti-lipid peroxidative effect, which may be useful in preventing the progress of various oxidative stress related diseases. The higher inhibitory effect of the extract could be attributed to its phytochemical content.

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Ameliorating reactive oxygen species-induced in vitro lipid peroxidation in brain, liver, mitochondria and DNA damage by Zingiber officinale Roscoe

Indian Journal of Clinical Biochemistry ◽

10.1007/s12291-010-0014-1 ◽

2010 ◽

Vol 25 (1) ◽

pp. 67-73 ◽

Cited By ~ 8

Author(s):

T. A. Ajith

Keyword(s):

Reactive Oxygen Species ◽

Lipid Peroxidation ◽

Dna Damage ◽

Reactive Oxygen ◽

Zingiber Officinale ◽

Liver Mitochondria ◽

Oxygen Species ◽

Zingiber Officinale Roscoe

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In vitro screen of inhibitors of rat brain serotonin synthesis

Canadian Journal of Biochemistry ◽

10.1139/o69-078 ◽

1969 ◽

Vol 47 (5) ◽

pp. 501-506 ◽

Cited By ~ 26

Author(s):

E. G. McGeer ◽

D. A. V. Peters

Keyword(s):

Rat Brain ◽

Tryptophan Hydroxylase ◽

Inhibitory Effect ◽

Complexing Agents ◽

Serotonin Synthesis ◽

Structural Requirements ◽

Class A ◽

Numerous Data ◽

Comparison Of The Results

Over 700 compounds were screened at 10−4 M concentration as inhibitors of the conversion of L-tryptophan-14C to serotonin-14C in crude rat brain homogenates. Most of the compounds had little or no inhibitory effect. Those with strong inhibitory properties were tested as inhibitors of 5-hydroxytryptophan decarboxylase and, if active on the decarboxylase, were assayed as tryptophan hydroxylase inhibitors. Except for a few oxidizing and complexing agents and for some substituted p-phenylenediamines, the compounds found to inhibit tryptophan hydroxylase by >50% belonged to the three types of inhibitors already known, i.e. catechols, phenylalanine and ring-substituted phenylalanines, and 6-substituted tryptophans. The numerous data in this screen make possible some comments as to the structural requirements for activity within each class. A comparison of the results on tryptophan hydroxylase with data on tyrosine hydroxylase inhibition in similar homogenates makes it clear that two separate, if somewhat similar, enzymes are involved.

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Alterations in Microrhizome Induction, Shoot Multiplication and Rooting of Ginger (Zingiber officinale Roscoe) var. Bentong with Regards to Sucrose and Plant Growth Regulators Application

Agronomy ◽

10.3390/agronomy11020320 ◽

2021 ◽

Vol 11 (2) ◽

pp. 320

Author(s):

Nisar Ahmad Zahid ◽

Hawa Z.E. Jaafar ◽

Mansor Hakiman

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Zingiber Officinale ◽

Naphthaleneacetic Acid ◽

Ms Medium ◽

Zingiber Officinale Roscoe ◽

Planting Materials ◽

Disease Free

Ginger (Zingiber officinale Roscoe) var. Bentong is a monocotyledon plant that belongs to the Zingiberaceae family. Bentong ginger is the most popular cultivar of ginger in Malaysia, which is conventionally propagated by its rhizome. As its rhizomes are the economic part of the plant, the allocation of a large amount of rhizomes as planting materials increases agricultural input cost. Simultaneously, the rhizomes’ availability as planting materials is restricted due to the high demand for fresh rhizomes in the market. Moreover, ginger propagation using its rhizome is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied to produce disease-free planting materials of ginger to overcome these problems. Hence, the in vitro-induced microrhizomes are considered as alternative disease-free planting materials for ginger cultivation. On the other hand, Bentong ginger has not been studied for its microrhizome induction. Therefore, this study was conducted to optimize sucrose and plant growth regulators (PGRs) for its microrhizome induction. Microrhizomes were successfully induced in Murashige and Skoog (MS) medium supplemented with a high sucrose concentration (>45 g L−1). In addition, zeatin at 5–10 µM was found more effective for microrhizome induction than 6-benzylaminopurine (BAP) at a similar concentration. The addition of 7.5 µM 1-naphthaleneacetic acid (NAA) further enhanced microrhizome formation and reduced sucrose’s required dose that needs to be supplied for efficient microrhizome formation. MS medium supplemented with 60 g L−1 sucrose, 10 µM zeatin and 7.5 µM NAA was the optimum combination for the microrhizome induction of Bentong ginger. The in vitro-induced microrhizomes sprouted indoors in moist sand and all the sprouted microrhizomes were successfully established in field conditions. In conclusion, in vitro microrhizomes can be used as disease-free planting materials for the commercial cultivation of Bentong ginger.

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