scholarly journals INHIBITORY EFFECT OF A STANDARDIZED HYDROETHANOLIC EXTRACT OF TERMINALIA ARJUNA BARK ON ALPHA-AMYLASE ENZYMEINHIBITORY EFFECT OF A STANDARDIZED HYDROETHANOLIC EXTRACT OF TERMINALIA ARJUNA BARK ON ALPHA-AMYLASE ENZYME

2018 ◽  
Vol 11 (4) ◽  
pp. 366 ◽  
Author(s):  
Sushant A Shengule ◽  
Sanjay Mishra ◽  
Shweta Bodhale
Keyword(s):  
Inhibitory Activity ◽  
High Performance ◽  
Soxhlet Extraction ◽  
Inhibitory Effect ◽  
Alpha Amylase ◽  
Antidiabetic Activity ◽  
Bark Extract ◽  
Terminalia Arjuna ◽  
Hydroethanolic Extract

 Objective: The present study was initiated to screen the hydroethanolic bark extract for α-amylase inhibitory activity and standardization of the Terminalia arjuna for polyphenolic phytochemicals using high-performance liquid chromatography-photo diode array (HPLC-PDA) method.Methods: The T. arjuna bark sample was extracted with ethanol: water (70:30 v/v) using Soxhlet extraction. A Dionex P680 HPLC system was used to acquire chromatograms. The screening of extract of T. arjuna bark has performed for in vitro α-amylase inhibitory assay. Each experiment was repeated 3 times. All values were expressed mean ± standard deviation.Results: The content of arjunetin, arjungenin, gallic acid, ellagic acid, and quercetin was 0.47, 8.22, 2.443, 7.901, and 3.20 mg/g, respectively, in a hydroethanolic extract of T. arjuna. The hydroethanolic extract of T. arjuna bark and acarbose has shown an inhibitory activity with an IC50 value 145.90 and 62.35 μg/mL, respectively.Conclusion: The hydroethanolic extract T. arjuna bark demonstrates α-amylase inhibitory activity due to a synergistic effect of the phytochemical constituents present in it. This study suggests that one of the mechanisms of this plant for antidiabetic activity is through the inhibition of α-amylase enzyme.

scholarly journals Assessment of In Vitro Antidiabetic Potential of Purified Anthocyanin Extract from Floral Petals of Wild Balsam Species

2020 ◽  
Vol 10 (3) ◽  
pp. 31-35
Author(s):  
R ARATHY ◽  
K MURUGAN ◽  
KV DINESH BABU ◽  
GS MANOJ
Keyword(s):  
International Diabetes Federation ◽  
Inhibitory Effect ◽  
Alpha Amylase ◽  
Herbal Remedies ◽  
Antidiabetic Activity ◽  
Public Health Issue ◽  
Impatiens Balsamina ◽  
Alpha Glucosidase

Diabetes is a notorious and growing clinical and public health issue. The International Diabetes Federation assumes that 592 million had diabetes by 2035 and that by 2040 the number will increase to 642 million. Cardiovascular corollary accounts for four million deaths annually attributable to diabetes. Evidence reveals that certain glucose-lowering phytochemicals can improve vascular outcomes with type 2 diabetes, which, together with better understanding of using multiple therapies concurrently, offers opportunities for beneficial personalization of medication regimens. Anthocyanins are coloured pigments and are natural antioxidants. Keeping this in focus, this study was undertaken to evaluate the in vitro antidiabetic activity in the petals of wild Impatiens balsamina L. The anthocyanin was extracted from floral petals of wild balsam species and purified to homogeneity using chromatographic techniques. Evaluation of in vitro antidiabetic properties of anthocyanin extract revealed a dose-dependent increase in the inhibitory effect on the alpha-glucosidase (200 μg/ml) and alpha-amylase enzymes (500 μg/ml) and was comparable with the standard acarbose drug (189 μg/ml and 50 μg/ml). These results indicated that anthocyanin could be used as a source of functional food and nutraceuticals. This information from wild species will be useful in finding more potent antidiabetic principle from the natural resources for the clinical development of antidiabetic therapeutics. Future studies are planned to substantiate the antidiabetic power of anthocyanin using in vivo animal models. Keywords: Alpha amylase, alpha glucosidase, diabetes, herbal remedies, Impatiens balsamina L.

scholarly journals EVALUATION OF α-GLUCOSIDASE INHIBITORY POTENTIAL OF METHANOLIC LEAF EXTRACT OF OCIMUM CANUM

2018 ◽  
Vol 10 (1) ◽  
pp. 126
Author(s):  
Kumari Smita
Keyword(s):  
High Performance ◽  
Leaf Extract ◽  
Soxhlet Extraction ◽  
Inhibitory Effect ◽  
Polyphenolic Compounds ◽  
Glucosidase Activity ◽  
Glucosidase Inhibitors ◽  
Vitro Model ◽  
Inhibitory Potential

Objective: The present investigation was designed to study the inhibitory effects of methanolic leaf extract of Ocimum canum (O. canum) on α-glucosidase using in vitro model followed by an assessment of bioactive compounds.Methods: The methanolic leaf extract was prepared by Soxhlet extraction method and partially purified by thin layer chromatography (TLC). Each band was subjected to α-glucosidase inhibition study. The positive bands were further characterized by high-performance liquid chromatography (HPLC) and quadrupole time of flight (Q-TOF) micro mass spectrometer.Results: Out of the several combinations of solvent systems, toluene, ethyl acetate and formic acid combination in the ratio of 7:2:1 revealed 5 bands on the TLC sheet. Among all the TLC bands, 2 bands (band A and B) showed the significant inhibitory effect on α-glucosidase activity. HPLC analysis of band A and B revealed the presence of two important polyphenolic compounds, namely rosmarinic acid (RA) and ursolic acid (UA). Q-TOF micromass spectrometer analysis revealed the percentage availability of RA, caffeic acid, tartaric acid, quercetin and other polyphenolic components in the bioactive bands.Conclusion: The study revealed that methanolic leaf extract of O. canum exhibits potent inhibition of α-glucosidase activity. Inhibition of α-glucosidase activity might be attributed to the presence of the polyphenolic compounds like RA and UA. Therefore, this finding can lead to the development of natural α-glucosidase inhibitors by the O. canum leaf extract.

scholarly journals In-Vitro Antidiabetic Effect of Ziziphus mucronata Leave Extracts

2021 ◽  
Vol 11 (6-S) ◽  
pp. 9-13
Author(s):  
Olaitan Kabir Abolaji ◽  
A.N. Ukwuani-Kwaja ◽  
I Sani ◽  
M.N. Sylvester
Keyword(s):  
Glucose Production ◽  
Inhibitory Effect ◽  
Alpha Amylase ◽  
Antidiabetic Activity ◽  
Anthraquinone Glycosides ◽  
Enzymes Inhibition ◽  
Ziziphus Mucronata ◽  
Dose Dependent Increase ◽  
Dose Dependent

Background: Diabetes is a metabolic disorder characterized by hyperglycemia due to the body’s inability to produce insulin or inaction of the produced insulin or a combination of both. One antidiabetic therapeutic approach is to reduce gastrointestinal glucose production and absorption through the inhibition of carbohydrate digesting enzymes such as alpha-amylase as well as through the inhibition of hemoglobin glycosylation. Objective: This study sets out to evaluate the in vitro antidiabetic activity of Ziziphus mucronata extracts for their effect on alpha-amylase and glycosylation of hemoglobin. Methods: Successive gradient maceration of Z. mucronata leaves were carried out using Hexane, Acetone, Methanol and separately with water to obtain four (4) extracts labelled HE, AE, ME, and WE respectively. These were subjected to in vitro studies for their inhibitory effect on alpha-amylase and hemoglobin glycosylation, Standard laboratory methods were used to screen for phytochemicals of the most potent extract. Results: The result showed that AE, ME and WE extract exhibited a dose-dependent increase in percentage inhibition of both alpha-amylase and hemoglobin glycosylation. However, on a stricking note, the AE showed a more potent data result with percentage (%) potency of 71.02 at 1mg/ml, the lowest glucose concentration (of 25mg/ml) at 0.242nm as well as the highest hemoglobin glycosylation inhibitory mean concentration of 3.663nm after 72 hours. The AE of Z. mucronata (the most potent) revealed the presence of alkaloids, anthraquinone, glycosides, flavonoids, phenols, saponin, tanins and terpenoids. Conclusion: Thus, the Acetone extract is more likely to give a lead antidiabetic drug molecule of drug when further explored; which somewhat justify the folkloric claims of Z. mucronata leave as an antidiabetic. Keywords: Heamoglabin glycosylation, Alpha-amylase enzymes, Inhibition, Glucose.

In-vitro Antioxidant and Antihyperglycemic effect of Muntingia calabura L. fruit extracts

2021 ◽  
pp. 6496-6502
Author(s):  
S. Sowmya ◽  
A. Jayaprakash
Keyword(s):  
Inhibitory Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Soxhlet Extraction ◽  
Hypoglycemic Agents ◽  
Antidiabetic Activity ◽  
Fruit Extract ◽  
Muntingia Calabura

This study investigated the in-vitro antioxidant activity and antidiabetic effect of Muntingia calabura fruit extract by in-vitro α-amylase and α-glucosidase inhibitory activity. Muntingia calabura fruit was extracted with aqueous methanol by soxhlet extraction. The total phenols and total flavonoids contents were estimated and evaluated for antioxidant activities (DPPH, ABTS) and in-vitro antidiabetic activity by measuring their inhibitory activity on α-amylase and α-glucosidase levels. The findings showed that the fruit extract had high content of total phenol and exhibited moderate free radical scavenging activity. The fruit extract showed inhibitory effect on α-amylase [IC50Value =61.43 μg/mL] and α-glucosidase [IC50 Value=140.33 μg/mL] compared to standard acarbose. The fruit extract can be used as a potential source for the development of new hypoglycemic agents may be due to the presence of high phenol content.

scholarly journals Antidiabetic Activity of Terminalia sericea Constituents

2011 ◽  
Vol 6 (11) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Nolitha Nkobole ◽  
Peter James Houghton ◽  
Ahmed Hussein ◽  
Namrita Lall
Keyword(s):  
Inhibitory Activity ◽  
Antioxidant Activities ◽  
Vero Cells ◽  
Stem Bark ◽  
Psidium Guajava ◽  
Antidiabetic Activity ◽  
Bark Extract ◽  
Traditional Use ◽  
Sclerocarya Birrea

Diabetes mellitus is an endocrine disorder that affects more than 100 million people worldwide. South African plants namely Terminalia sericea, Euclea natalensis, Warbugia salutaris, Aloe ferox, Artemisia afra, Sclerocarya birrea, Spirostachys africana and Psidium guajava were investigated for their in vitro α-glucosidase and α-amylase properties, and antioxidant activities. Terminalia sericea stem bark extract showed the best results against α-glucosidase and α-amylase enzymes. Bioassay-guided fractionation of an acetone extract of T. sericea stem bark led to the isolation of four known compounds namely β-sitosterol (1), β-sitosterol-3-acetate (2), lupeol (3), and stigma-4-ene-3-one (4), in addition to two inseparable sets of mixtures of isomers [epicatechin-catechin (M1), and gallocatechin-epigallocatechin (M2). 1 and 3 showed the best inhibitory activity on α-glucosidase (IC50:54.5 and 66.5 μM). Bio-evaluation of the inhibitory activity of the purified compounds on α-amylase showed that 3 and 1 exhibited IC50 values of 140.7 and 216.02 μM, respectively against α-amylase. Compounds 2, M1, 3 and M2 were found to be nontoxic to Vero cells. This study is the first to report α-glucosidase and α-amylase activity of M1, M2, 2 and 4 isolated from T. sericea, which validated the traditional use of the bark of T. sericea for diabetes in South Africa.

scholarly journals In Vitro Antidiabetic Effect of Neohesperidin

2019 ◽  
Vol 9 (6) ◽  
pp. 102-109 ◽  
Author(s):  
Durgeshnandani Sinha ◽  
Trilochan Satapathy ◽  
Parag Jain ◽  
Jhakeshwar Prasad Chandel ◽  
Divya Sahu ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Amylase Activity ◽  
Inhibitory Effect ◽  
Alpha Amylase ◽  
Rat Intestine ◽  
Antidiabetic Effect ◽  
Metabolizing Enzyme ◽  
Different Sources ◽  
Alpha Glucosidase

Objective: The present study was performed to determine in vitro antidiabetic effect of neohesperidin. To evaluate inhibitory effect of neohesperidin on α-amylase and α-glucosidase diabetes causing enzyme. Methods and Materials: Invitro carbohydrate metabolizing enzyme based inhibitory methods were used to determine antidiabetic effect of neohesperidin. Alpha (α)-amylase inhibitory assay was performed using different sources i.e. wheat alpha (α)-amylase enzyme, salivary alpha (α)-amylase and fungal alpha (α)-amylase assay. Alpha (α)-glucosidase inhibitory assay was performed using alpha (α)-glucosidase (B. stearothermophil), alpha (α)-glucosidase rat intestine and alpha (α)-glucosidase from baker’s yeast. Sucrase inhibitory assay from rat small intestine. Result: Neohesperidin possess a potent anti-diabetic by significantly inhibiting alpha amylase activity. Conclusion: It was concluded that enzyme inhibitory activity of neohesperidin shown a significantly higher inhibitory activity on alpha-amylase in comparision to alpha-glucosidase & Sucrase enzymes. Keywords: Neohespiridin, acarbose, alpha-amylase, alpha-glucosidase

scholarly journals α-Amylase and α-Glucosidase inhibitory activity assessment of Cucurbita maxima seeds – a LIBS based study

2016 ◽  
Vol 8 (3) ◽  
pp. 312 ◽  
Author(s):  
Devesh Kumar Kushawaha ◽  
Manjulika Yadav ◽  
Sanjukta Chatterji ◽  
Amrita Kumari Srivastava ◽  
Geeta Watal
Keyword(s):  
Inhibitory Activity ◽  
Inhibitory Effect ◽  
Postprandial Hyperglycemia ◽  
Antidiabetic Activity ◽  
Cucurbita Maxima ◽  
Breakdown Spectroscopy ◽  
Activity Assessment ◽  
Laser Induced Breakdown ◽  
Hydrolyzing Enzymes

The aim of the present study was to investigate, α-amylase and α-glucosidase inhibitory activities of the aqueous extract of <em>Cucurbita maxima</em> seeds <em>in vitro</em> and to correlate their activity with their phytoelemental profile quantitatively assessed by Laser-Induced Breakdown Spectroscopy (LIBS). Diabetes can be managed by controlling postprandial hyperglycemia which can be achieved by inhibiting carbohydrate hydrolyzing enzymes like α-amylase and α-glucosidase. Results reveal that the <em>C. maxima</em> seeds have appreciable α-amylase inhibitory activity of 46.03±1.37% with IC<sub>50</sub> value at 7.00±0.29 mg ml<sup>-1</sup> in addition to substantial α-glucosidase inhibitory effect of 35.11±1.04% with IC<sub>50</sub> at 8.11±0.36 mg ml<sup>-1</sup>. Acarbose was used as a reference. LIBS analysis showed the presence of certain phytoelements viz. Mg, Ca, K and Na which are well known glycemic elements and hence could be responsible for inhibitory activity of carbohydrate hydrolyzing enzymes.Thus, α-amylase and α-glucosidase inhibitory action of <em>C. maxima</em> seeds would be responsible for their antidiabetic activity. The glycemic elemental profile further validates their role in controlling diabetes and hence <em>C. maxima</em> seeds could be explored as a potential herbal candidate for managing postprandial hyperglycemia causing type 2 diabetes mellitus.

scholarly journals Complex Tannins Isolated from Jelly Fig Achenes Affect Pectin Gelation through Non-Specific Inhibitory Effect on Pectin Methylesterase

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1601 ◽  
Author(s):  
Shang-Ta Wang ◽  
You-Jiang Feng ◽  
Ying-Jang Lai ◽  
Nan-Wei Su
Keyword(s):  
Inhibitory Activity ◽  
Digestive Enzymes ◽  
High Performance ◽  
Pectin Methylesterase ◽  
Inhibitory Effect ◽  
Specific Protein ◽  
Liquid Chromatography Mass Spectrometry ◽  
Binding Agent ◽  
Crude Aqueous Extract

Jelly fig (Ficus awkeotsang Makino) is used to prepare drinks and desserts in Asia, owing to the gelling capability of its pectin via endogenous pectin methylesterase (PE) catalyzation. Meanwhile, substances with PE inhibitory activity (SPEI) in jelly fig achenes (JFA) residue were noticed to be able to impede the gelation. In this study, we characterized and isolated SPEI from JFA by a series of PE inhibition-guided isolations. Crude aqueous extract of JFA residue was mixed with acetone, and 90% acetone-soluble matter was further fractionated by Diaion HP-20 chromatography. The retained fraction with dominant PE inhibitory activity was collected from 100% methanol eluate. Results from high-performance liquid chromatography mass spectrometry (HPLC/MS) and hydrolysis-induced chromogenic transition revealed the SPEI as complex tannins. Total tannins content was determined in each isolated fraction, and was closely related to PE inhibitory activity. In addition, SPEI in this study could inhibit activities of digestive enzymes in vitro and may, therefore, be assumed to act as non-specific protein binding agent.

scholarly journals Ingol and Ingenol-Type Diterpenes from Euphorbia trigona Miller with Keratinocyte Inhibitory Activity

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1206
Author(s):  
Reham Hammadi ◽  
Norbert Kúsz ◽  
Csilla Zsuzsanna Dávid ◽  
Zoltán Behány ◽  
László Papp ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Cytotoxic Activity ◽  
Inhibitory Activity ◽  
High Performance ◽  
Skin Condition ◽  
Positive Control ◽  
Active Species ◽  
The European Union ◽  
Ingenol Mebutate

Ingenol mebutate, isolated from Euphorbia peplus, is an ingenane-type diterpenoid, primarily used for the topical treatment of actinic keratosis, a premalignant skin condition. The aim of our work was to investigate other Euphorbia species to find structurally similar diterpenes that can be used as alternatives to ingenol mebutate. Pharmacological investigation of Euphorbia candelabrum, Euphorbia cotinifolia, Euphorbia ramipressa, and Euphorbia trigona revealed the potent keratinocyte (HPV-Ker cell line) inhibitory activity of these spurge species. From the methanolic extract of the aerial parts of Euphorbia trigona Miller, the most active species, five ingol (1–5) and four ingenane-type diterpenoids (6–9) were isolated by various chromatographic separation techniques, including open column chromatography, vacuum liquid chromatography, thin-layer chromatography, and high-performance liquid chromatography. The structures of the compounds were determined by NMR spectroscopic analysis and by comparison of the assignations with the literature data. The cytotoxic activity of the compounds against keratinocytes was tested in vitro by using ingenol mebutate as a positive control. Among the isolated compounds, two ingenane derivatives (6 and 7) exhibited remarkably stronger cytotoxic activity (IC50 values 0.39 μM and 0.32 μM, respectively) on keratinocytes than ingenol mebutate (IC50 value 0.84 μM). These compounds could serve as starting materials for further investigations to find alternatives to Picato® (with active substance ingenol mebutate), which was withdrawn from marketing authorization in the European Union.

scholarly journals Development of mouthwash with Rosmarinus officinalis extract

2014 ◽  
Vol 50 (4) ◽  
pp. 851-858 ◽  
Author(s):  
Isabela Moreira Baumgratz de Paula ◽  
Flávia Costa Moraes ◽  
Orlando Vieira de Souza ◽  
Célia Hitomi Yamamoto
Keyword(s):  
Sodium Fluoride ◽  
Inhibitory Activity ◽  
Raw Materials ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Rosmarinus Officinalis ◽  
Hexane Fraction ◽  
Diffusion Method ◽  
Oral Infections

Rosmarinus officinalis, which belongs to the Lamiaceaefamily, is a species of medicinal flora with therapeutic properties. In order to exploit the benefits of these properties, a mouthwash formulation was developed, with careful selection of raw materials to meet pharmacotechnical requirements. Extracts of the plant were incorporated into a mouthwash, which was shown to have inhibitory action in vitro against the micro-organisms commonly found in periodontics. Controls for assessing the quality of the drugs were carried out, quantifying phenols and flavonoids as chemical markers. Mouthwash solutions were formulated containing 0.1, 5 and 10% ethanol extract of R. officinalis; and 0.05, 5 and 10% of the hexane fraction of R. officinalis. In order to evaluate synergism, ethanol extract and hexane fraction were also added to formulations containing 0.05% sodium fluoride and 0.12% chlorhexidine digluconate. These formulations were assessed for inhibitory effect against the specific microorganisms involved in the process of bacterial plaque formation, S. mutans(ATCC25175) and C. albicans(ATCC 10231), frequently found in cases of oral infections. The agar diffusion method was used to evaluate the inhibitory activity of extracts and formulations. All mouthwash solutions displayed inhibitory activity having higher sensitivity to S. mutansfor the 5% ethanol extract+0.05% sodium fluoride, and greater sensitivity to C. albicansfor the 10% hexane fraction. Results were characterized by the appearance of a growth inhibition halo, justifying the utilization and association of extracts of R. officinalis.

Sign in / Sign up

Export Citation Format

Share Document