scholarly journals Lycium barbarum polysaccharide attenuates cisplatininduced apoptosis in ovary granulosa cells via alleviation of endoplasmic reticulum stress

2016 ◽  
Vol 16 (4) ◽  
pp. 827 ◽  
Author(s):  
Li-Qiong Huang ◽  
Yuan-Zhen Zhang ◽  
Bo Zheng ◽  
Yi He
Keyword(s):  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Granulosa Cells ◽  
Lycium Barbarum

scholarly journals NRF2-mediated signaling is a master regulator of transcription factors in bovine granulosa cells under oxidative stress condition

2021 ◽  
Author(s):  
Mohamed Omar Taqi ◽  
Mohammed Saeed-Zidane ◽  
Samuel Gebremedhn ◽  
Dessie Salilew-Wondim ◽  
Ernst Tholen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Proliferation ◽  
Endoplasmic Reticulum ◽  
Transcription Factors ◽  
Endoplasmic Reticulum Stress ◽  
Granulosa Cells ◽  
Mitochondrial Activity ◽  
Small Interference Rna ◽  
Nrf2 Signaling Pathway

AbstractTranscription factors (TFs) are known to be involved in regulating the expression of several classes of genes during folliculogenesis. However, the regulatory role of TFs during oxidative stress (OS) is not fully understood. The current study was aimed to investigate the regulation of the TFs in bovine granulosa cells (bGCs) during exposure to OS induced by H2O2 in vitro. For this, bGCs derived from ovarian follicles were cultured in vitro till their confluency and then treated with H2O2 for 40 min. Twenty-four hours later, cells were subjected to various phenotypic and gene expression analyses for genes related to TFs, endoplasmic reticulum stress, apoptosis, cell proliferation, and differentiation markers. The bGCs exhibited higher reactive oxygen species accumulation, DNA fragmentation, and endoplasmic reticulum stress accompanied by reduction of mitochondrial activity after exposure to OS. In addition, higher lipid accumulation and lower cell proliferation were noticed in H2O2-challenged cells. The mRNA level of TFs including NRF2, E2F1, KLF6, KLF9, FOS, SREBF1, SREBF2, and NOTCH1 was increased in H2O2-treated cells compared with non-treated controls. However, the expression level of KLF4 and its downstream gene, CCNB1, were downregulated in the H2O2-challenged group. Moreover, targeted inhibition of NRF2 using small interference RNA resulted in reduced expression of KLF9, FOS, SREBF2, and NOTCH1 genes, while the expression of KLF4 was upregulated. Taken together, bovine granulosa cells exposed to OS exhibited differential expression of various transcription factors, which are mediated by the NRF2 signaling pathway.

Boron Attenuates Heat Stress–Induced Apoptosis by Inhibiting Endoplasmic Reticulum Stress in Mouse Granulosa Cells

2020 ◽  
Vol 199 (2) ◽  
pp. 611-621 ◽  
Author(s):  
Yongjie Xiong ◽  
Erhui Jin ◽  
Qirun Yin ◽  
Chuanyan Che ◽  
Shaojun He
Keyword(s):  
Endoplasmic Reticulum ◽  
Heat Stress ◽  
Endoplasmic Reticulum Stress ◽  
Granulosa Cells ◽  
Induced Apoptosis

scholarly journals Endoplasmic Reticulum Stress Activated by Androgen Enhances Apoptosis of Granulosa Cells via Induction of Death Receptor 5 in PCOS

Endocrinology ◽  
2018 ◽  
Vol 160 (1) ◽  
pp. 119-132 ◽  
Author(s):  
Jerilee M K Azhary ◽  
Miyuki Harada ◽  
Nozomi Takahashi ◽  
Emi Nose ◽  
Chisato Kunitomi ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Granulosa Cells ◽  
Death Receptor ◽  
Death Receptor 5
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